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The map label for this gene is pbpB [H]

Identifier: 229918546

GI number: 229918546

Start: 2794705

End: 2796852

Strand: Direct

Name: pbpB [H]

Synonym: EAT1b_2833

Alternate gene names: 229918546

Gene position: 2794705-2796852 (Clockwise)

Preceding gene: 229918545

Following gene: 229918547

Centisome position: 93.16

GC content: 49.07

Gene sequence:

>2148_bases
ATGAATTCTTTAAAACAGAGTAAAAAAAGAGTGGCGTGGGTAGCGATTGTCTGCTTCACCCCGCTCTTTTTATTTTTTGT
CGGATGGTTCCTTTATTTATCCGTCACCCATACGTACAAAGGGGAAAACATGCTCGCATTCGCGGAAGAGCAGCGCTGGA
AGGCGGTCAGTACGCTAGAGGCGGAACGGGGCGAGGTGTATGATCGCTTCGGTCAACCGATTGCCATCAACATTCCGTCA
TACCGGCTCGTCGCCGTCTTGAAACTCGGCGGTGAACGGGTCGATGGGAAGACACTCATGCCCGATGAATTCGCCGAAGC
GGCGAAGCAATTGTCGACCGTGCTTCCTATGACAGAAGCTGATTTATTGAAACGGCTTGAACAAAATGCCGACCTTGGGC
AGATCGAGTTCGGTGTTCCCGGGCGTGATTTGACCGTAGAACAAAAAGAAGAGATTGAAGCATTCAATCTTAACGGCATC
ACGTTCGTCGAGGAACCGAAGCGCTATTATCCGAACAAAGTATTTGCATCCTCGGTGATCGGGTATGCGCAGAAGATTGA
CCAGAACGGTCGCATCGAACTCGTCGGCGAACTCGGGATTGAAAAGTCTCTGAACGATATTTTAAGTGAATCGTACGGTC
TGTATCGATTTGAGAAAGATTTGGCGGGTCGTGAACTCGTATCGAGTGACGAGCAGTTGAAGATTGAACCGGAAGACGGG
GCGGATGTCCAATTGACCATCGACCACCGAATCCAGCAGTTGCTCGAAACGAAGATGAATGAGTTGTATGAAGAGTATGA
GCCGAAACATGCGACGGCCATCATCATGGACCCGAAAACAGGAGAGATCATTGCACTGTCGGATCGACCATCGTTTGACC
CGAACACACGCGAGATTTCTTCATACAGTAACTTTTCTGTCTCGTCTCGATTTGAACCTGGGTCGACGATGAAGATCTTT
ACGCTTGCAGCAGCCATCAACGAGGGCGTGTATAACCCGAATGAGTTGTATGAATCCGGTTCGTACAAATATGGTAAGAC
GGTCTTCAATGATTACAACATTACGGGATGGGGTACCATTCCTATGGTAGAAGGCGTCTGGCATTCGTCGAACGTCGCAT
TCTCGATTTTAGCGAACGAACGACTTGGTCTCAGTCGATATGAAGATTACTTCAAAGCGTTCAAGTTTGATCAGCGGACA
GGGATCGATTTGCCTGGAGAGGTCAATAGTCAATTCGATTTCGAAAGTCCGATGAACGTCTTGATCACGGCATGGGGACA
ATCGACAGCGGTCACACCGATGCAAATCTTGCAAGCGACATCGGCCATCGCGACAGATGGAACGATGAAGCAACCGCACG
TCATCAAAAAAATCGTCAATAGCGAGACCGGCGAAACGATTCGGACGACCAATCCGAAAGAAGTCGGTCAACCGATCACG
GCGGAAACGGCGAAAAAGACGCGTGAAGCGCTCGACGGTGTCGTCAATAGCGACGTGGGAACCGGGCAACTGTATGCGTT
AGACAACTATCGCGTCGTCGGAAAGACCGGTACCGCCCAAATCGCCGAGAACGGAAAATATTTGAGCGGACAGTATATTC
ATTCGTTCGTCGGGATGGCTCCAGAAGACGATCCGGAACTTGTCATGTATATCGCAGTGGACAGACCGAACAGTGACTCG
TCTACGACCGGTCCACGAATCATGGCGCCACTTTTCAAAGACGTGATGGATGTCGCGTTACGTTACCGGAGTGTCGAGCC
TGAAAAACAAGAAAAAGCGGTCGAAGCCTCGACGAAGACAACGTCTTCTTATATCGGTCAAAACCGTGAAGAGGCAGTGA
ACGCATCGACGGAGAATTCCTTCAATCCGATCGTGTTAGGTGATGGAAACGAAGTGATCGCGCAGAGTCCTGTGAAAGGA
CAATCGTATGTCGTCGGTGAACGATTGTTGCTAAAAACGAGCGAGTCGTTTACGATGCCTACATTAAAAGGTTGGTCGTT
ACGTGACGTCAAACGTTTAGCGACCCTTTATCAATTAAATTTAGAAATCGACGGTACCGGATTTGTCACATCGCAATCGA
TTAGCAAAGGGAAACCGGTCAAAACAGGTGCCTCCTTGAAAGTGAAGTTGGAGACACCGTCGAATTAA

Upstream 100 bases:

>100_bases
AGTATGAAATCGCCCAATTAAGCTCGCCCGAAAGAATTTATGCGATTGCCGAGAAGCTCGGAATGAAATTCAATGAAAAG
AATGTCAAGGTGATTGATTG

Downstream 100 bases:

>100_bases
CGCACGAGATGAAGAGGGCTGCGGCTCTCTTTTCCTATGTAAGAGGAGGAAGTTTGTATGGAACAACTGACAGTTCAAAA
ATTAGCATCATGGTTTGGGA

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 715; Mature: 715

Protein sequence:

>715_residues
MNSLKQSKKRVAWVAIVCFTPLFLFFVGWFLYLSVTHTYKGENMLAFAEEQRWKAVSTLEAERGEVYDRFGQPIAINIPS
YRLVAVLKLGGERVDGKTLMPDEFAEAAKQLSTVLPMTEADLLKRLEQNADLGQIEFGVPGRDLTVEQKEEIEAFNLNGI
TFVEEPKRYYPNKVFASSVIGYAQKIDQNGRIELVGELGIEKSLNDILSESYGLYRFEKDLAGRELVSSDEQLKIEPEDG
ADVQLTIDHRIQQLLETKMNELYEEYEPKHATAIIMDPKTGEIIALSDRPSFDPNTREISSYSNFSVSSRFEPGSTMKIF
TLAAAINEGVYNPNELYESGSYKYGKTVFNDYNITGWGTIPMVEGVWHSSNVAFSILANERLGLSRYEDYFKAFKFDQRT
GIDLPGEVNSQFDFESPMNVLITAWGQSTAVTPMQILQATSAIATDGTMKQPHVIKKIVNSETGETIRTTNPKEVGQPIT
AETAKKTREALDGVVNSDVGTGQLYALDNYRVVGKTGTAQIAENGKYLSGQYIHSFVGMAPEDDPELVMYIAVDRPNSDS
STTGPRIMAPLFKDVMDVALRYRSVEPEKQEKAVEASTKTTSSYIGQNREEAVNASTENSFNPIVLGDGNEVIAQSPVKG
QSYVVGERLLLKTSESFTMPTLKGWSLRDVKRLATLYQLNLEIDGTGFVTSQSISKGKPVKTGASLKVKLETPSN

Sequences:

>Translated_715_residues
MNSLKQSKKRVAWVAIVCFTPLFLFFVGWFLYLSVTHTYKGENMLAFAEEQRWKAVSTLEAERGEVYDRFGQPIAINIPS
YRLVAVLKLGGERVDGKTLMPDEFAEAAKQLSTVLPMTEADLLKRLEQNADLGQIEFGVPGRDLTVEQKEEIEAFNLNGI
TFVEEPKRYYPNKVFASSVIGYAQKIDQNGRIELVGELGIEKSLNDILSESYGLYRFEKDLAGRELVSSDEQLKIEPEDG
ADVQLTIDHRIQQLLETKMNELYEEYEPKHATAIIMDPKTGEIIALSDRPSFDPNTREISSYSNFSVSSRFEPGSTMKIF
TLAAAINEGVYNPNELYESGSYKYGKTVFNDYNITGWGTIPMVEGVWHSSNVAFSILANERLGLSRYEDYFKAFKFDQRT
GIDLPGEVNSQFDFESPMNVLITAWGQSTAVTPMQILQATSAIATDGTMKQPHVIKKIVNSETGETIRTTNPKEVGQPIT
AETAKKTREALDGVVNSDVGTGQLYALDNYRVVGKTGTAQIAENGKYLSGQYIHSFVGMAPEDDPELVMYIAVDRPNSDS
STTGPRIMAPLFKDVMDVALRYRSVEPEKQEKAVEASTKTTSSYIGQNREEAVNASTENSFNPIVLGDGNEVIAQSPVKG
QSYVVGERLLLKTSESFTMPTLKGWSLRDVKRLATLYQLNLEIDGTGFVTSQSISKGKPVKTGASLKVKLETPSN
>Mature_715_residues
MNSLKQSKKRVAWVAIVCFTPLFLFFVGWFLYLSVTHTYKGENMLAFAEEQRWKAVSTLEAERGEVYDRFGQPIAINIPS
YRLVAVLKLGGERVDGKTLMPDEFAEAAKQLSTVLPMTEADLLKRLEQNADLGQIEFGVPGRDLTVEQKEEIEAFNLNGI
TFVEEPKRYYPNKVFASSVIGYAQKIDQNGRIELVGELGIEKSLNDILSESYGLYRFEKDLAGRELVSSDEQLKIEPEDG
ADVQLTIDHRIQQLLETKMNELYEEYEPKHATAIIMDPKTGEIIALSDRPSFDPNTREISSYSNFSVSSRFEPGSTMKIF
TLAAAINEGVYNPNELYESGSYKYGKTVFNDYNITGWGTIPMVEGVWHSSNVAFSILANERLGLSRYEDYFKAFKFDQRT
GIDLPGEVNSQFDFESPMNVLITAWGQSTAVTPMQILQATSAIATDGTMKQPHVIKKIVNSETGETIRTTNPKEVGQPIT
AETAKKTREALDGVVNSDVGTGQLYALDNYRVVGKTGTAQIAENGKYLSGQYIHSFVGMAPEDDPELVMYIAVDRPNSDS
STTGPRIMAPLFKDVMDVALRYRSVEPEKQEKAVEASTKTTSSYIGQNREEAVNASTENSFNPIVLGDGNEVIAQSPVKG
QSYVVGERLLLKTSESFTMPTLKGWSLRDVKRLATLYQLNLEIDGTGFVTSQSISKGKPVKTGASLKVKLETPSN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=595, Percent_Identity=25.3781512605042, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1786854, Length=524, Percent_Identity=26.3358778625954, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 79463; Mature: 79463

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLKQSKKRVAWVAIVCFTPLFLFFVGWFLYLSVTHTYKGENMLAFAEEQRWKAVSTLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHH
AERGEVYDRFGQPIAINIPSYRLVAVLKLGGERVDGKTLMPDEFAEAAKQLSTVLPMTEA
HHCCHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
DLLKRLEQNADLGQIEFGVPGRDLTVEQKEEIEAFNLNGITFVEEPKRYYPNKVFASSVI
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCEECCCCEEEEECCHHHCCCHHHHHHHH
GYAQKIDQNGRIELVGELGIEKSLNDILSESYGLYRFEKDLAGRELVSSDEQLKIEPEDG
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHHCCHHHHCCCCEEEECCCCC
ADVQLTIDHRIQQLLETKMNELYEEYEPKHATAIIMDPKTGEIIALSDRPSFDPNTREIS
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHH
SYSNFSVSSRFEPGSTMKIFTLAAAINEGVYNPNELYESGSYKYGKTVFNDYNITGWGTI
CCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCC
PMVEGVWHSSNVAFSILANERLGLSRYEDYFKAFKFDQRTGIDLPGEVNSQFDFESPMNV
CCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LITAWGQSTAVTPMQILQATSAIATDGTMKQPHVIKKIVNSETGETIRTTNPKEVGQPIT
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHCCCCC
AETAKKTREALDGVVNSDVGTGQLYALDNYRVVGKTGTAQIAENGKYLSGQYIHSFVGMA
HHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCHHHCCCCEECHHHHHHHHCCC
PEDDPELVMYIAVDRPNSDSSTTGPRIMAPLFKDVMDVALRYRSVEPEKQEKAVEASTKT
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TSSYIGQNREEAVNASTENSFNPIVLGDGNEVIAQSPVKGQSYVVGERLLLKTSESFTMP
HHHHHCCCHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCEEEECCEEEEECCCCCCCC
TLKGWSLRDVKRLATLYQLNLEIDGTGFVTSQSISKGKPVKTGASLKVKLETPSN
CCCCCCHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MNSLKQSKKRVAWVAIVCFTPLFLFFVGWFLYLSVTHTYKGENMLAFAEEQRWKAVSTLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHHHHHHHHHHH
AERGEVYDRFGQPIAINIPSYRLVAVLKLGGERVDGKTLMPDEFAEAAKQLSTVLPMTEA
HHCCHHHHHCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHH
DLLKRLEQNADLGQIEFGVPGRDLTVEQKEEIEAFNLNGITFVEEPKRYYPNKVFASSVI
HHHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCEECCCCEEEEECCHHHCCCHHHHHHHH
GYAQKIDQNGRIELVGELGIEKSLNDILSESYGLYRFEKDLAGRELVSSDEQLKIEPEDG
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCEEEHHHHCCHHHHCCCCEEEECCCCC
ADVQLTIDHRIQQLLETKMNELYEEYEPKHATAIIMDPKTGEIIALSDRPSFDPNTREIS
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCCCCHHHH
SYSNFSVSSRFEPGSTMKIFTLAAAINEGVYNPNELYESGSYKYGKTVFNDYNITGWGTI
CCCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCHHHHHCCCCCCCCCEEECCCCCCCCCCC
PMVEGVWHSSNVAFSILANERLGLSRYEDYFKAFKFDQRTGIDLPGEVNSQFDFESPMNV
CCCCCEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEE
LITAWGQSTAVTPMQILQATSAIATDGTMKQPHVIKKIVNSETGETIRTTNPKEVGQPIT
EEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHCCCCC
AETAKKTREALDGVVNSDVGTGQLYALDNYRVVGKTGTAQIAENGKYLSGQYIHSFVGMA
HHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEEECCCCCHHHCCCCEECHHHHHHHHCCC
PEDDPELVMYIAVDRPNSDSSTTGPRIMAPLFKDVMDVALRYRSVEPEKQEKAVEASTKT
CCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TSSYIGQNREEAVNASTENSFNPIVLGDGNEVIAQSPVKGQSYVVGERLLLKTSESFTMP
HHHHHCCCHHHHCCCCCCCCCCCEEECCCCCEEECCCCCCCEEEECCEEEEECCCCCCCC
TLKGWSLRDVKRLATLYQLNLEIDGTGFVTSQSISKGKPVKTGASLKVKLETPSN
CCCCCCHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]