Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 229918544

GI number: 229918544

Start: 2793386

End: 2794318

Strand: Direct

Name: mraW

Synonym: EAT1b_2831

Alternate gene names: 229918544

Gene position: 2793386-2794318 (Clockwise)

Preceding gene: 229918543

Following gene: 229918545

Centisome position: 93.12

GC content: 48.55

Gene sequence:

>933_bases
ATGTTTGAGCATGAAACGGTTTTAAAAATGGAGTCCATTGAGGGATTGAACATCAAACCAGATGGGGTATATGTCGATTG
TACGTTAGGTGGAGCGGGTCATAGTGAAGAAATCGCAAAGCGTCTAACGACGGGTCAACTATATGCGTTCGACCAAGATG
ACGTCGCGCTCGCACATGCGAAAGAGCGATTGGCTCCTTATGAAGGCCGTGTGACGTTTATAAAGAGTAATTTCGTTCAC
TTGAAGGAAGAACTCGCCCAACGTGGTGTGACAAAAGTGGACGGTGTACTATTCGACCTAGGCGTCTCTTCGCCACAGCT
AGATGAAGCGGAGCGCGGGTTCAGTTACAACTATGACGCACCGCTCGATATGAGGATGGACCGGTCTCGGGACTTTTCAG
CCTATCATGTTGTGAATGAATGGTCGTTTGGAGAACTTGCTCGTATTTTCTTTACATACGGGGAGGAAAAGTTTTCAAAG
CAAATCGCACGAAAGATTGAGCAAGCCCGTGCCGATAAACCGATTGAGACGACATTCGAGCTTGTCGACTTGATTAAAGA
AGCGATTCCTGCACCAGCTAGACGAAAAGGTGGACACCCGGCGAAGCGGACGTTCCAAGCGATTCGAATCGCCGTCAATG
ACGAGTTGAACGTATTCGACCGCGCTGTTCAAGATGCGATTGATTTACTCGCAGTGAACGGACGCATCTGTGTCATCACG
TTCCATTCGTTAGAAGACCGCATTTGTAAACAAGTGTTCAAAGAGCGTAGCCAGCATCCACCGTTACCACCGGGGTTGCC
GGTCATTCCGAAAGAATTTGAACCGGAACTCAAACTCATCACCCGTAAGCCGATCGTGGCGAGTGAGGGAGAACTTGAAG
ATAACCGTCGTTCACGCTCCGCTAAGCTGAGAGTGGCCGAAAAACAAAAATAA

Upstream 100 bases:

>100_bases
TTTCCTCTTTTCACTAAAAACCTGTACGGACCTTGACATTTTTATGATAAAGTAAGTGTAATAAAACAGTTGAAATATGT
AGGTTGAGAGGGTTTTTTGA

Downstream 100 bases:

>100_bases
CCGAGAGTGTAGGAGGGATACCTGATGCCAGAAGCTGCCCGTAAGGCGTTGCAGCCTCAAGTAACACCGAATCGAAATCC
GATTCCGCAAGAACGACCAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MFEHETVLKMESIEGLNIKPDGVYVDCTLGGAGHSEEIAKRLTTGQLYAFDQDDVALAHAKERLAPYEGRVTFIKSNFVH
LKEELAQRGVTKVDGVLFDLGVSSPQLDEAERGFSYNYDAPLDMRMDRSRDFSAYHVVNEWSFGELARIFFTYGEEKFSK
QIARKIEQARADKPIETTFELVDLIKEAIPAPARRKGGHPAKRTFQAIRIAVNDELNVFDRAVQDAIDLLAVNGRICVIT
FHSLEDRICKQVFKERSQHPPLPPGLPVIPKEFEPELKLITRKPIVASEGELEDNRRSRSAKLRVAEKQK

Sequences:

>Translated_310_residues
MFEHETVLKMESIEGLNIKPDGVYVDCTLGGAGHSEEIAKRLTTGQLYAFDQDDVALAHAKERLAPYEGRVTFIKSNFVH
LKEELAQRGVTKVDGVLFDLGVSSPQLDEAERGFSYNYDAPLDMRMDRSRDFSAYHVVNEWSFGELARIFFTYGEEKFSK
QIARKIEQARADKPIETTFELVDLIKEAIPAPARRKGGHPAKRTFQAIRIAVNDELNVFDRAVQDAIDLLAVNGRICVIT
FHSLEDRICKQVFKERSQHPPLPPGLPVIPKEFEPELKLITRKPIVASEGELEDNRRSRSAKLRVAEKQK
>Mature_310_residues
MFEHETVLKMESIEGLNIKPDGVYVDCTLGGAGHSEEIAKRLTTGQLYAFDQDDVALAHAKERLAPYEGRVTFIKSNFVH
LKEELAQRGVTKVDGVLFDLGVSSPQLDEAERGFSYNYDAPLDMRMDRSRDFSAYHVVNEWSFGELARIFFTYGEEKFSK
QIARKIEQARADKPIETTFELVDLIKEAIPAPARRKGGHPAKRTFQAIRIAVNDELNVFDRAVQDAIDLLAVNGRICVIT
FHSLEDRICKQVFKERSQHPPLPPGLPVIPKEFEPELKLITRKPIVASEGELEDNRRSRSAKLRVAEKQK

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=335, Percent_Identity=36.1194029850746, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=39.6825396825397, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=43.6305732484076, Blast_Score=243, Evalue=1e-65,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=39.2749244712991, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=39.2749244712991, Blast_Score=179, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_EXISA (C4L5T9)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002887190.1
- ProteinModelPortal:   C4L5T9
- GeneID:   7870718
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2831
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35138; Mature: 35138

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 51-51 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEHETVLKMESIEGLNIKPDGVYVDCTLGGAGHSEEIAKRLTTGQLYAFDQDDVALAHA
CCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHH
KERLAPYEGRVTFIKSNFVHLKEELAQRGVTKVDGVLFDLGVSSPQLDEAERGFSYNYDA
HHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCHHHCCCCCCCCC
PLDMRMDRSRDFSAYHVVNEWSFGELARIFFTYGEEKFSKQIARKIEQARADKPIETTFE
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVDLIKEAIPAPARRKGGHPAKRTFQAIRIAVNDELNVFDRAVQDAIDLLAVNGRICVIT
HHHHHHHHCCCCHHHCCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHEEECCEEEEEE
FHSLEDRICKQVFKERSQHPPLPPGLPVIPKEFEPELKLITRKPIVASEGELEDNRRSRS
ECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHH
AKLRVAEKQK
HEEEECCCCC
>Mature Secondary Structure
MFEHETVLKMESIEGLNIKPDGVYVDCTLGGAGHSEEIAKRLTTGQLYAFDQDDVALAHA
CCCCHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCEEEEECCCCHHHHHH
KERLAPYEGRVTFIKSNFVHLKEELAQRGVTKVDGVLFDLGVSSPQLDEAERGFSYNYDA
HHHCCCCCCEEEEEECCHHHHHHHHHHCCCHHHCCEEEECCCCCCCCCHHHCCCCCCCCC
PLDMRMDRSRDFSAYHVVNEWSFGELARIFFTYGEEKFSKQIARKIEQARADKPIETTFE
CCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHH
LVDLIKEAIPAPARRKGGHPAKRTFQAIRIAVNDELNVFDRAVQDAIDLLAVNGRICVIT
HHHHHHHHCCCCHHHCCCCHHHHHHHHHEEEECCCHHHHHHHHHHHHHHEEECCEEEEEE
FHSLEDRICKQVFKERSQHPPLPPGLPVIPKEFEPELKLITRKPIVASEGELEDNRRSRS
ECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHH
AKLRVAEKQK
HEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA