Click here to switch to the map view.

The map label for this gene is tgt [H]

Identifier: 229918409

GI number: 229918409

Start: 2660484

End: 2661629

Strand: Direct

Name: tgt [H]

Synonym: EAT1b_2694

Alternate gene names: 229918409

Gene position: 2660484-2661629 (Clockwise)

Preceding gene: 229918408

Following gene: 229918410

Centisome position: 88.69

GC content: 52.27

Gene sequence:

>1146_bases
ATGACAAAACATGCAGTCACATACGAACACATCCACACATGCAAACAGACCGGAGCGCGGCACGGAATCGTCCATACGCC
GCACGGGTCGTTCGAGACACCGGTCTTCATGCCGGTCGGGACGCAAGCGAGCGTCAAGACGATGGCACCTGAACAGGTCA
AGGCGATGGGTGCGAACATTATCTTGTCGAACACGTATCATCTCTGGGTTCGTCCAGGACATGAGGTCATCAAACAAGCG
GGTGGACTTCATAAGTTCATGAACTGGGACGGGGCAATCCTCACGGACTCGGGTGGATTCCAAGTGTTCTCACTGGCCGA
CCTCCGAAACATTCAAGAGGAAGGCGTACATTTCCGTAACCATTTGAACGGCGACAAGTTGTTCTTGTCACCAGAAAAGG
CGATGGAGATTCAAAACGCGCTCGGCTCGGACATCATGATGGCATTCGACGAGTGCCCGCCTTATCCGGCGACGCATGAA
TACATGAAGAAGTCAGTCGAGCGGACGAGCCGTTGGGCGGAACGTTGCCTCGAGGCACACGAACGTCCAGAAGACCAGGC
GCTCTTCGGCATCATCCAAGGTGGAGAATATGAGGACCTTCGTCGTCAAAGCGCCCAAGACCTCGTCTCACTTGATTTCC
CAGGATATGCGGTCGGTGGTCTCTCGGTCGGTGAACCGAAAGACGTCATGTATCGTGCCCTCGAATTCACGACGCCGCTC
ATGCCAGAGAACAAACCGCGTTATTTGATGGGCGTCGGCTCACCTGATGCGTTAATTGAAGGATCGATTCGCGGAATCGA
CATGTTCGACTGTGTCCTTCCGACACGTATTGCACGAAACGGAACGCTCATGACGTCGAAAGGTCGCGTCACAATCAAAC
AAGCGAAGTACAAGACGGACTTCGGTCCAATCGATGAGAAGTGTGACTGTAACACGTGTAAGAATTACTCGCGTGCGTAC
GTTCACCACTTGATTCGAGCGGATGAGATGTTTGGTCTTCACTTGTGTACGACACACAATCTTCACTTCCTCGTGAAGTT
GATGGAGCAGGTTCGTCAAGCGATTCGTGAAGACCGCTTGCTCGATTTCAAAGAAGAGTTCTTTGAAGAATACGGCTACA
ACAAGCCGAACGCGAAGAATTTCTAA

Upstream 100 bases:

>100_bases
GCGACATCATCTTACACGCTTATCGGACTGCGGTCGCAGAACGATACCGCTTCTTCAGCTTTGGAGACGCGATGCTCATC
ACGAGGAAGGAAGTAACAAA

Downstream 100 bases:

>100_bases
GCAATCATGTCGGACATTGTGTGACAATGCTTCAGTTTCACGGTATAATGAACCTGATATTGAAGGAAAGGGTGAATGTT
ACTCATGGAAACAATCGTTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MTKHAVTYEHIHTCKQTGARHGIVHTPHGSFETPVFMPVGTQASVKTMAPEQVKAMGANIILSNTYHLWVRPGHEVIKQA
GGLHKFMNWDGAILTDSGGFQVFSLADLRNIQEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPATHE
YMKKSVERTSRWAERCLEAHERPEDQALFGIIQGGEYEDLRRQSAQDLVSLDFPGYAVGGLSVGEPKDVMYRALEFTTPL
MPENKPRYLMGVGSPDALIEGSIRGIDMFDCVLPTRIARNGTLMTSKGRVTIKQAKYKTDFGPIDEKCDCNTCKNYSRAY
VHHLIRADEMFGLHLCTTHNLHFLVKLMEQVRQAIREDRLLDFKEEFFEEYGYNKPNAKNF

Sequences:

>Translated_381_residues
MTKHAVTYEHIHTCKQTGARHGIVHTPHGSFETPVFMPVGTQASVKTMAPEQVKAMGANIILSNTYHLWVRPGHEVIKQA
GGLHKFMNWDGAILTDSGGFQVFSLADLRNIQEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPATHE
YMKKSVERTSRWAERCLEAHERPEDQALFGIIQGGEYEDLRRQSAQDLVSLDFPGYAVGGLSVGEPKDVMYRALEFTTPL
MPENKPRYLMGVGSPDALIEGSIRGIDMFDCVLPTRIARNGTLMTSKGRVTIKQAKYKTDFGPIDEKCDCNTCKNYSRAY
VHHLIRADEMFGLHLCTTHNLHFLVKLMEQVRQAIREDRLLDFKEEFFEEYGYNKPNAKNF
>Mature_380_residues
TKHAVTYEHIHTCKQTGARHGIVHTPHGSFETPVFMPVGTQASVKTMAPEQVKAMGANIILSNTYHLWVRPGHEVIKQAG
GLHKFMNWDGAILTDSGGFQVFSLADLRNIQEEGVHFRNHLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPATHEY
MKKSVERTSRWAERCLEAHERPEDQALFGIIQGGEYEDLRRQSAQDLVSLDFPGYAVGGLSVGEPKDVMYRALEFTTPLM
PENKPRYLMGVGSPDALIEGSIRGIDMFDCVLPTRIARNGTLMTSKGRVTIKQAKYKTDFGPIDEKCDCNTCKNYSRAYV
HHLIRADEMFGLHLCTTHNLHFLVKLMEQVRQAIREDRLLDFKEEFFEEYGYNKPNAKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=358, Percent_Identity=46.6480446927374, Blast_Score=358, Evalue=4e-99,
Organism=Homo sapiens, GI13375872, Length=286, Percent_Identity=27.2727272727273, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1786607, Length=359, Percent_Identity=58.2172701949861, Blast_Score=432, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=48.5875706214689, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI25146502, Length=245, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=45.2830188679245, Blast_Score=348, Evalue=2e-96,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=26.171875, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 43188; Mature: 43057

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHAVTYEHIHTCKQTGARHGIVHTPHGSFETPVFMPVGTQASVKTMAPEQVKAMGANI
CCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEE
ILSNTYHLWVRPGHEVIKQAGGLHKFMNWDGAILTDSGGFQVFSLADLRNIQEEGVHFRN
EEECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPATHEYMKKSVERTSRWAERCLEAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERPEDQALFGIIQGGEYEDLRRQSAQDLVSLDFPGYAVGGLSVGEPKDVMYRALEFTTPL
CCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHCCCC
MPENKPRYLMGVGSPDALIEGSIRGIDMFDCVLPTRIARNGTLMTSKGRVTIKQAKYKTD
CCCCCCCEEEECCCCCHHEECCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCC
FGPIDEKCDCNTCKNYSRAYVHHLIRADEMFGLHLCTTHNLHFLVKLMEQVRQAIREDRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHC
LDFKEEFFEEYGYNKPNAKNF
CHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TKHAVTYEHIHTCKQTGARHGIVHTPHGSFETPVFMPVGTQASVKTMAPEQVKAMGANI
CCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEECCCCCCHHHCCHHHHHHCCCEE
ILSNTYHLWVRPGHEVIKQAGGLHKFMNWDGAILTDSGGFQVFSLADLRNIQEEGVHFRN
EEECCEEEEECCCHHHHHHCCCHHHHHCCCCEEEECCCCEEEEEHHHHHHHHHHCCHHHH
HLNGDKLFLSPEKAMEIQNALGSDIMMAFDECPPYPATHEYMKKSVERTSRWAERCLEAH
CCCCCEEEECHHHHHHHHHHHCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ERPEDQALFGIIQGGEYEDLRRQSAQDLVSLDFPGYAVGGLSVGEPKDVMYRALEFTTPL
CCCCCCEEEEEECCCCHHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHCCCC
MPENKPRYLMGVGSPDALIEGSIRGIDMFDCVLPTRIARNGTLMTSKGRVTIKQAKYKTD
CCCCCCCEEEECCCCCHHEECCCCCCCHHHHHHHHHHHCCCCEEECCCCEEEEECCCCCC
FGPIDEKCDCNTCKNYSRAYVHHLIRADEMFGLHLCTTHNLHFLVKLMEQVRQAIREDRL
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHC
LDFKEEFFEEYGYNKPNAKNF
CHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA