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The map label for this gene is mrpE [H]

Identifier: 229918335

GI number: 229918335

Start: 2587515

End: 2588000

Strand: Direct

Name: mrpE [H]

Synonym: EAT1b_2620

Alternate gene names: 229918335

Gene position: 2587515-2588000 (Clockwise)

Preceding gene: 229918334

Following gene: 229918336

Centisome position: 86.25

GC content: 46.09

Gene sequence:

>486_bases
ATGGCTTATCAACTATTGATTAACTTTTTCTTGGCGTTCATCTGGATGTTCCTCACGGGATCGTTCACAACGAGCGGATT
CTTCGTCGGGTATCTACTCGGCTTACTCATCTTGTTCATGATGCGCCGATTCTTTAAATCTAGTGGGGGCGGTTTTTACT
TCACACGCGTCTGGCGTCTGTTTAAACTGCTTCTCATCTTCATCCGTGAATTGTTCCTCGCGAACTTTGAGGTACTGCGT
CTCGTCCTTAGTCCGAAGTTGAAAATCCAACCAGGGATTTTCCGCTATGAGACGTCGCTCGACAAAGGATGGAAAGTCAG
CTTGCTCTCGATGCTGATCACGCTCACACCAGGGACGCTCGTCGTTCAGGTGTCAAAAGACAATTCCGTGCTCTATATCC
ACGCACTTAACATGCCGGATAAAGAAGCACTACAAAAAGACATTTATGATAACTTTGAATCAAGCATTAAGGAGGCGACA
GAGTGA

Upstream 100 bases:

>100_bases
TTTGGGGCAGAAGCCATCCATCCGTATATCACGCAAGCGATTGAGCCGCTCGTCAATCCAGAAATCTATATCGATGCCGT
GATAAAGGAGTGAATGACAG

Downstream 100 bases:

>100_bases
TGGATTTACTAGACATTCTCATCTACATCTCGCTCGCCATTCTCTTGCTTGCGATGTTGATGATGTTGTATCGCGTCGTC
AAAGGACCGACGACGGCAGA

Product: cation antiporter

Products: NA

Alternate protein names: Mrp complex subunit E; Multiple resistance and pH homeostasis protein E [H]

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MAYQLLINFFLAFIWMFLTGSFTTSGFFVGYLLGLLILFMMRRFFKSSGGGFYFTRVWRLFKLLLIFIRELFLANFEVLR
LVLSPKLKIQPGIFRYETSLDKGWKVSLLSMLITLTPGTLVVQVSKDNSVLYIHALNMPDKEALQKDIYDNFESSIKEAT
E

Sequences:

>Translated_161_residues
MAYQLLINFFLAFIWMFLTGSFTTSGFFVGYLLGLLILFMMRRFFKSSGGGFYFTRVWRLFKLLLIFIRELFLANFEVLR
LVLSPKLKIQPGIFRYETSLDKGWKVSLLSMLITLTPGTLVVQVSKDNSVLYIHALNMPDKEALQKDIYDNFESSIKEAT
E
>Mature_160_residues
AYQLLINFFLAFIWMFLTGSFTTSGFFVGYLLGLLILFMMRRFFKSSGGGFYFTRVWRLFKLLLIFIRELFLANFEVLRL
VLSPKLKIQPGIFRYETSLDKGWKVSLLSMLITLTPGTLVVQVSKDNSVLYIHALNMPDKEALQKDIYDNFESSIKEATE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 18713; Mature: 18581

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYQLLINFFLAFIWMFLTGSFTTSGFFVGYLLGLLILFMMRRFFKSSGGGFYFTRVWRL
CHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FKLLLIFIRELFLANFEVLRLVLSPKLKIQPGIFRYETSLDKGWKVSLLSMLITLTPGTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEE
VVQVSKDNSVLYIHALNMPDKEALQKDIYDNFESSIKEATE
EEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYQLLINFFLAFIWMFLTGSFTTSGFFVGYLLGLLILFMMRRFFKSSGGGFYFTRVWRL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
FKLLLIFIRELFLANFEVLRLVLSPKLKIQPGIFRYETSLDKGWKVSLLSMLITLTPGTL
HHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCCHHHHHHHHHHHCCCCEE
VVQVSKDNSVLYIHALNMPDKEALQKDIYDNFESSIKEATE
EEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 10198001; 11356194 [H]