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The map label for this gene is mrpD [H]

Identifier: 229918334

GI number: 229918334

Start: 2586023

End: 2587507

Strand: Direct

Name: mrpD [H]

Synonym: EAT1b_2619

Alternate gene names: 229918334

Gene position: 2586023-2587507 (Clockwise)

Preceding gene: 229918333

Following gene: 229918335

Centisome position: 86.2

GC content: 52.79

Gene sequence:

>1485_bases
ATGATTAACTTACCGGTCTTTCCAATCGTCATTCCTGCGCTTGCGGCCATCCTTATGATGTTTATGCCAAAACGGGTCCA
GCTGCAGCGCGCGATGGCTCTTGGAGCTTTAGTTATGACGGCGATCGTGTCACTCTTTCTCGTGCATACGGTCTCCACGG
AGGGGATTCAAATCCTCAACCTCGGGAACTGGCAAGCGCCGTTCGGCATCACGCTCGTATCGGATATGCTGTCAGCCCTA
CTCGTGACGACGTCGACTCTTCTCACGCTCTTCATCGTGTGGTTCGCCGTCCATTATTTCTCAGATTCATATGAAGGCAA
CTACTTGTACATCTCGATGCTGTTCTTGCTCGTCGGGGTGAACGGTGCCTTCACGACAGGTGATATTTTCAACTTGTTCG
TCTTCTTCGAGGTCTTCTTGATGTCCTCATACGTGTTGATTGTACTCGGTGGACGTGGCGTCCAGTTACGTGAATCGATC
AAGTACTTGCTCGTGAACATCATCGCCTCGGCGCTCTTCGTCATGTCGGTCGCCTTGCTTTACGGAGTCACAGGCACGCT
CAACTTGGCGGACTTGGCGATGAAGATCCCGCTCGTCGATAACCCGGACGTCATCACCGTCATCGGTGTGCTGTTCGTCA
TCGTCTTCGGGATGAAAGGGGCACTCGTCCCACTTCACTACTGGCTCCCTGGAGCGTACGTGGTCGCACCGACCCCGATT
CTCGCCATGTTTGGGGCACTCTTGACGAAAGTCGGGGTGTACTCGATCTTGCGGACGTACACGCTACTGTTTGACGGGAA
CGGTGAATTTTTACAGACGTTCCTCATGGTGCTCGCTGTCCTCACCATCTTCATCGGGATGACAGGGGCCATCGCCTATA
ACGATGTGAAACTGATCATCATCTACAACATCATGATCGCGGTCGGGGTCATCTTGTACGGAATCTCGGTCAATACGCAG
ACGTCCCTTGAAGGGTCGCTTTACTACTTGATTCACGACATGATCATCAAAGCCGTTCTCTTCATGCTCGTCGGGATGAT
GATCGGGATCACCAAATCCGGTCAACTTCGCGATATGGGTGGACTCATCACCCGATTCCCGCTGTTCGGATGGACGTTCT
TCGTCGCAGCGCTCTCGCTCGCTGGGATTCCGCCGCTTAGTGGATTCTTCGGGAAATTGTTGATCGTCTCAGGCGGGATG
GACGAAGGGGAACTGTTCGGGCCACTTCTCGTCCTCTTGTCAAGTCTCTTCGTCCTCTATTCGGTCATGAAGATTTTCTT
GAACGGGTTCTGGGGCGAGGCGAAGCGCGAGTATGATGGACCGCTCGTACCGTATACGAAGCGTTTGATTGTACCGGTCT
TCTTGTTGCTCATCATTGCGGTCGCATACGGATTTGGGGCAGAAGCCATCCATCCGTATATCACGCAAGCGATTGAGCCG
CTCGTCAATCCAGAAATCTATATCGATGCCGTGATAAAGGAGTGA

Upstream 100 bases:

>100_bases
ATCAGTTTCGGGGTGACGGCGTTCTTCCTCGTACTTGCTTACCGCACGTACCAGGAGCTCAGCACGGACAATATGGAAGA
AATGAAAGGAACAGAATCGT

Downstream 100 bases:

>100_bases
ATGACAGATGGCTTATCAACTATTGATTAACTTTTTCTTGGCGTTCATCTGGATGTTCCTCACGGGATCGTTCACAACGA
GCGGATTCTTCGTCGGGTAT

Product: NADH/Ubiquinone/plastoquinone (complex I)

Products: NA

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MINLPVFPIVIPALAAILMMFMPKRVQLQRAMALGALVMTAIVSLFLVHTVSTEGIQILNLGNWQAPFGITLVSDMLSAL
LVTTSTLLTLFIVWFAVHYFSDSYEGNYLYISMLFLLVGVNGAFTTGDIFNLFVFFEVFLMSSYVLIVLGGRGVQLRESI
KYLLVNIIASALFVMSVALLYGVTGTLNLADLAMKIPLVDNPDVITVIGVLFVIVFGMKGALVPLHYWLPGAYVVAPTPI
LAMFGALLTKVGVYSILRTYTLLFDGNGEFLQTFLMVLAVLTIFIGMTGAIAYNDVKLIIIYNIMIAVGVILYGISVNTQ
TSLEGSLYYLIHDMIIKAVLFMLVGMMIGITKSGQLRDMGGLITRFPLFGWTFFVAALSLAGIPPLSGFFGKLLIVSGGM
DEGELFGPLLVLLSSLFVLYSVMKIFLNGFWGEAKREYDGPLVPYTKRLIVPVFLLLIIAVAYGFGAEAIHPYITQAIEP
LVNPEIYIDAVIKE

Sequences:

>Translated_494_residues
MINLPVFPIVIPALAAILMMFMPKRVQLQRAMALGALVMTAIVSLFLVHTVSTEGIQILNLGNWQAPFGITLVSDMLSAL
LVTTSTLLTLFIVWFAVHYFSDSYEGNYLYISMLFLLVGVNGAFTTGDIFNLFVFFEVFLMSSYVLIVLGGRGVQLRESI
KYLLVNIIASALFVMSVALLYGVTGTLNLADLAMKIPLVDNPDVITVIGVLFVIVFGMKGALVPLHYWLPGAYVVAPTPI
LAMFGALLTKVGVYSILRTYTLLFDGNGEFLQTFLMVLAVLTIFIGMTGAIAYNDVKLIIIYNIMIAVGVILYGISVNTQ
TSLEGSLYYLIHDMIIKAVLFMLVGMMIGITKSGQLRDMGGLITRFPLFGWTFFVAALSLAGIPPLSGFFGKLLIVSGGM
DEGELFGPLLVLLSSLFVLYSVMKIFLNGFWGEAKREYDGPLVPYTKRLIVPVFLLLIIAVAYGFGAEAIHPYITQAIEP
LVNPEIYIDAVIKE
>Mature_494_residues
MINLPVFPIVIPALAAILMMFMPKRVQLQRAMALGALVMTAIVSLFLVHTVSTEGIQILNLGNWQAPFGITLVSDMLSAL
LVTTSTLLTLFIVWFAVHYFSDSYEGNYLYISMLFLLVGVNGAFTTGDIFNLFVFFEVFLMSSYVLIVLGGRGVQLRESI
KYLLVNIIASALFVMSVALLYGVTGTLNLADLAMKIPLVDNPDVITVIGVLFVIVFGMKGALVPLHYWLPGAYVVAPTPI
LAMFGALLTKVGVYSILRTYTLLFDGNGEFLQTFLMVLAVLTIFIGMTGAIAYNDVKLIIIYNIMIAVGVILYGISVNTQ
TSLEGSLYYLIHDMIIKAVLFMLVGMMIGITKSGQLRDMGGLITRFPLFGWTFFVAALSLAGIPPLSGFFGKLLIVSGGM
DEGELFGPLLVLLSSLFVLYSVMKIFLNGFWGEAKREYDGPLVPYTKRLIVPVFLLLIIAVAYGFGAEAIHPYITQAIEP
LVNPEIYIDAVIKE

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=315, Percent_Identity=26.3492063492064, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI251831116, Length=356, Percent_Identity=22.752808988764, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788829, Length=414, Percent_Identity=27.7777777777778, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788613, Length=470, Percent_Identity=27.4468085106383, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788827, Length=346, Percent_Identity=26.878612716763, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1788831, Length=288, Percent_Identity=21.5277777777778, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI2367154, Length=435, Percent_Identity=24.367816091954, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693160, Length=255, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788614, Length=429, Percent_Identity=23.7762237762238, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 54089; Mature: 54089

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLPVFPIVIPALAAILMMFMPKRVQLQRAMALGALVMTAIVSLFLVHTVSTEGIQILN
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LGNWQAPFGITLVSDMLSALLVTTSTLLTLFIVWFAVHYFSDSYEGNYLYISMLFLLVGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
NGAFTTGDIFNLFVFFEVFLMSSYVLIVLGGRGVQLRESIKYLLVNIIASALFVMSVALL
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVTGTLNLADLAMKIPLVDNPDVITVIGVLFVIVFGMKGALVPLHYWLPGAYVVAPTPI
HCCCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEECCCHH
LAMFGALLTKVGVYSILRTYTLLFDGNGEFLQTFLMVLAVLTIFIGMTGAIAYNDVKLII
HHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEHHH
IYNIMIAVGVILYGISVNTQTSLEGSLYYLIHDMIIKAVLFMLVGMMIGITKSGQLRDMG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLITRFPLFGWTFFVAALSLAGIPPLSGFFGKLLIVSGGMDEGELFGPLLVLLSSLFVLY
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHH
SVMKIFLNGFWGEAKREYDGPLVPYTKRLIVPVFLLLIIAVAYGFGAEAIHPYITQAIEP
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LVNPEIYIDAVIKE
HCCCCEEEEEEECC
>Mature Secondary Structure
MINLPVFPIVIPALAAILMMFMPKRVQLQRAMALGALVMTAIVSLFLVHTVSTEGIQILN
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LGNWQAPFGITLVSDMLSALLVTTSTLLTLFIVWFAVHYFSDSYEGNYLYISMLFLLVGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCC
NGAFTTGDIFNLFVFFEVFLMSSYVLIVLGGRGVQLRESIKYLLVNIIASALFVMSVALL
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVTGTLNLADLAMKIPLVDNPDVITVIGVLFVIVFGMKGALVPLHYWLPGAYVVAPTPI
HCCCCCCCHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEECCCHH
LAMFGALLTKVGVYSILRTYTLLFDGNGEFLQTFLMVLAVLTIFIGMTGAIAYNDVKLII
HHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCEEECCEEHHH
IYNIMIAVGVILYGISVNTQTSLEGSLYYLIHDMIIKAVLFMLVGMMIGITKSGQLRDMG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLITRFPLFGWTFFVAALSLAGIPPLSGFFGKLLIVSGGMDEGELFGPLLVLLSSLFVLY
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHH
SVMKIFLNGFWGEAKREYDGPLVPYTKRLIVPVFLLLIIAVAYGFGAEAIHPYITQAIEP
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LVNPEIYIDAVIKE
HCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]