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The map label for this gene is aroE

Identifier: 229918236

GI number: 229918236

Start: 2481670

End: 2482455

Strand: Direct

Name: aroE

Synonym: EAT1b_2517

Alternate gene names: 229918236

Gene position: 2481670-2482455 (Clockwise)

Preceding gene: 229918235

Following gene: 229918237

Centisome position: 82.73

GC content: 52.67

Gene sequence:

>786_bases
ATGAATCTGGCCGTCATCGGTCACCCGATTGCTCATTCGCTCTCTCCCCAACTGCACGAGCAGTGGTTGAGGGCGTCTGG
GCTTTTCGGGCGTTATGAGGCCATCGATGCCACACCAGATCAACTCCCAGCCTTATTTGCGGCAATGCGAGAAGGGGACT
GGGATGGATTCAATGTCACCATTCCCTATAAAGAGGTGGTCGTTCGGTATCTCGATGACCTTGACGAAGCGGCGAAACAT
GCTGGAGCCGTCAACACAGTGTATAAACGAGACGGGCGACTTATCGGGACAAATACGGACGGGGCAGGTCTTGTCCAAGC
GCTGATGCCATACACAGACTTTAGAGGGCATGTCCTGATTGTCGGTGCCGGAGGAGCGGCTCGGGGGATTGTCCAAGCTC
TCCCGACGCGTGACGTGACGATTGTCAATCGAACTGTGGAACGGGCAAAAGCGCTCGCCGACACGTTCGGAGTCGTCTAC
ACGACGTTTGATGAGATGGACGTCTCTCGATATGATGTCATCATTCAGACCACTTCTGTCGGGATGGACGAGAGATCGAC
ACCACTTTCATTAGAAGGATTACGACAAAACACGGTCGTTTGTGATATCATATATCGTCCACTTGTCACCCCGATGCTTC
AAGAAGCGAAAAGTCGCGGAGCAAATATTGTGACAGGTGTAGCCATGTTCGTCGGGCAGGGCGCGCTCTCGTTCGAGAAA
TGGACGGGCGTGAAACCGGACGAAACCGTTGGAAAAAAGTTAATCGAAGGATTATTGGAGGAATAA

Upstream 100 bases:

>100_bases
TGTTCAGCGGTCTCGGTTGGGTCGCGATTCACGGAAAAGGTGGCCGCATCGCAGCATGGGCTCCAAAAGGCGTCGCAGTC
ACACTTCGCGAGGCGCTCGT

Downstream 100 bases:

>100_bases
TATACTATGTTATCAGGTAAACAAAAACGCTTTTTACGCGCAGAAGCGCACCATCTCTCACCGATTTATCAAGTCGGGAA
GAATGGGGTCAGTGAAGCGA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MNLAVIGHPIAHSLSPQLHEQWLRASGLFGRYEAIDATPDQLPALFAAMREGDWDGFNVTIPYKEVVVRYLDDLDEAAKH
AGAVNTVYKRDGRLIGTNTDGAGLVQALMPYTDFRGHVLIVGAGGAARGIVQALPTRDVTIVNRTVERAKALADTFGVVY
TTFDEMDVSRYDVIIQTTSVGMDERSTPLSLEGLRQNTVVCDIIYRPLVTPMLQEAKSRGANIVTGVAMFVGQGALSFEK
WTGVKPDETVGKKLIEGLLEE

Sequences:

>Translated_261_residues
MNLAVIGHPIAHSLSPQLHEQWLRASGLFGRYEAIDATPDQLPALFAAMREGDWDGFNVTIPYKEVVVRYLDDLDEAAKH
AGAVNTVYKRDGRLIGTNTDGAGLVQALMPYTDFRGHVLIVGAGGAARGIVQALPTRDVTIVNRTVERAKALADTFGVVY
TTFDEMDVSRYDVIIQTTSVGMDERSTPLSLEGLRQNTVVCDIIYRPLVTPMLQEAKSRGANIVTGVAMFVGQGALSFEK
WTGVKPDETVGKKLIEGLLEE
>Mature_261_residues
MNLAVIGHPIAHSLSPQLHEQWLRASGLFGRYEAIDATPDQLPALFAAMREGDWDGFNVTIPYKEVVVRYLDDLDEAAKH
AGAVNTVYKRDGRLIGTNTDGAGLVQALMPYTDFRGHVLIVGAGGAARGIVQALPTRDVTIVNRTVERAKALADTFGVVY
TTFDEMDVSRYDVIIQTTSVGMDERSTPLSLEGLRQNTVVCDIIYRPLVTPMLQEAKSRGANIVTGVAMFVGQGALSFEK
WTGVKPDETVGKKLIEGLLEE

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=272, Percent_Identity=40.0735294117647, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI1787983, Length=265, Percent_Identity=30.9433962264151, Blast_Score=120, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6320332, Length=267, Percent_Identity=30.3370786516854, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_EXISA (C4L3U2)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886882.1
- ProteinModelPortal:   C4L3U2
- GeneID:   7868426
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2517
- OMA:   DMARTIG
- ProtClustDB:   CLSK2489116
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 28434; Mature: 28434

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: ACT_SITE 64-64

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAVIGHPIAHSLSPQLHEQWLRASGLFGRYEAIDATPDQLPALFAAMREGDWDGFNVT
CCEEEECCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
IPYKEVVVRYLDDLDEAAKHAGAVNTVYKRDGRLIGTNTDGAGLVQALMPYTDFRGHVLI
ECHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEEE
VGAGGAARGIVQALPTRDVTIVNRTVERAKALADTFGVVYTTFDEMDVSRYDVIIQTTSV
EECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCEEEEEEEECC
GMDERSTPLSLEGLRQNTVVCDIIYRPLVTPMLQEAKSRGANIVTGVAMFVGQGALSFEK
CCCCCCCCCCHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
WTGVKPDETVGKKLIEGLLEE
HCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLAVIGHPIAHSLSPQLHEQWLRASGLFGRYEAIDATPDQLPALFAAMREGDWDGFNVT
CCEEEECCHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEE
IPYKEVVVRYLDDLDEAAKHAGAVNTVYKRDGRLIGTNTDGAGLVQALMPYTDFRGHVLI
ECHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEECCCCCHHHHHHHHCCCCCCCCEEE
VGAGGAARGIVQALPTRDVTIVNRTVERAKALADTFGVVYTTFDEMDVSRYDVIIQTTSV
EECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHHHCCCCCEEEEEEEECC
GMDERSTPLSLEGLRQNTVVCDIIYRPLVTPMLQEAKSRGANIVTGVAMFVGQGALSFEK
CCCCCCCCCCHHCCCCCCEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHH
WTGVKPDETVGKKLIEGLLEE
HCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA