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The map label for this gene is cssS [H]

Identifier: 229918195

GI number: 229918195

Start: 2429249

End: 2430619

Strand: Direct

Name: cssS [H]

Synonym: EAT1b_2475

Alternate gene names: 229918195

Gene position: 2429249-2430619 (Clockwise)

Preceding gene: 229918194

Following gene: 229918196

Centisome position: 80.98

GC content: 48.07

Gene sequence:

>1371_bases
ATGATGGACGCGCTTAAACCGAAACGTCTCATGTGGAAGATTTGGGTGCTCATCTTACTCATCATCGTGACGCTTGCGCT
CATGATCTTGATTACGGTCCGTTCATCCATCACCCAGTTCATCGATGAACAAGTGTATGAGACGTTACGAGATGTGGACT
ATTATTTAGGGAACACGAATGAGTTAGAGAACTATCCCCAAAACCCGATTGAGTATGACCGACGCCAACAGGAGTCCCGT
TCGGTCAACCAATTGATCTTTCTCCCGAACGGACGGATCATCTACGGATCGGCCCCGCTTGAATTGACGAACCGCATGTA
TCAGGAAGCGTTAAATCAAAGTGGTCAGGTCGAGCGGTATCAGACGACGATTGATGGGGAAGACGTCTATTACATCATTC
GTCAAAATGAGTACGATGGAACGATTGTGTATCAGGCCTCCTACGCTTGGGATGCCTATCGTCGTGAGTTGGTGTCGACA
TTATATTTGCGAATATCGATTCTGATAGGTATCATCAGTCTCTTATCGCTTATCCCCGCCTTGTTGATGTCGCGGCAATT
GACGAAACCGATCGTGGAGATGGAACGGGCCGTTGAGAAGATTTCGAACCGACAATGGGAGACGGCTTTACCACTAGATC
GTCAAGATGAACTCGGCCAATTGGCTCACTCGATTGATGCCATGCGAAAAGAGTTGAAAGAGCAAGACCTGGCACAACAG
GCGTTGCTTCAAAACATATCGCATGACTTAAAGACACCGATCATGGTCATTCGAGGATATGCCCAATCGATTGGCGACGG
TATTTATCCGACAGGAGACTTGAAAGGTTCTGCCGAGGTCATTGATGAGGAAGCGTCTCGACTCGAAAAGAAAGTCGTCG
ATTTGTTGTACGTCACAAAACTTGAATATTTCAAGGGACAGGAAATGAAACTTGAACCAATTAATCTCGACAAGTTGATG
GAACTGCTCGTCGCGCGCTTCAAACTTAGAGGCCAGCTCGATTGGGAAATCAATGGCGAAGGCGGCGAGATGCTTGGAGA
CGGGGAACAAATCCGGGTCGCATTTGAGAACGTGCTCGACAATGCACTCCGTTACGCGGAATCTTGCATTCGCATCGACC
TCCACCAAGATGACGCTGGCGTTGAAATCACCATCTTTAACGACGGACCGCCAGTTGATGAGAGTTTAGACTTGTTCCAC
CAATTCTCTCGAGGGAAGCAAGGGAAGTTCGGACTCGGTCTGTTCATCGTGCAGCGAATTGTGACACTTCACACAGGGGA
AGTGTCTCTACGAAATACGGGAGACGGAACAGCAGTGCGATTTTACTTTCCCCGACTCAAGTCGAACCAACCTTTAAATT
CGAATGCTTGA

Upstream 100 bases:

>100_bases
CGGACCGCGTCGTCGATGATTTGGTGCGTCGTGTCCGAAAGAAATTGCATCGCCTTGAGCTCGAAACGCTTTACGGTATC
GGCTACCGTCTCGTCAGACC

Downstream 100 bases:

>100_bases
TTTTCAAAACCAGCCAGGAAACGGCTGGTTTTTTCATGGGAAATAGACAAAAGCCGATTTGCGGGGCGCATCGAATCCTG
GTATGAATGACATGTAAGTC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MMDALKPKRLMWKIWVLILLIIVTLALMILITVRSSITQFIDEQVYETLRDVDYYLGNTNELENYPQNPIEYDRRQQESR
SVNQLIFLPNGRIIYGSAPLELTNRMYQEALNQSGQVERYQTTIDGEDVYYIIRQNEYDGTIVYQASYAWDAYRRELVST
LYLRISILIGIISLLSLIPALLMSRQLTKPIVEMERAVEKISNRQWETALPLDRQDELGQLAHSIDAMRKELKEQDLAQQ
ALLQNISHDLKTPIMVIRGYAQSIGDGIYPTGDLKGSAEVIDEEASRLEKKVVDLLYVTKLEYFKGQEMKLEPINLDKLM
ELLVARFKLRGQLDWEINGEGGEMLGDGEQIRVAFENVLDNALRYAESCIRIDLHQDDAGVEITIFNDGPPVDESLDLFH
QFSRGKQGKFGLGLFIVQRIVTLHTGEVSLRNTGDGTAVRFYFPRLKSNQPLNSNA

Sequences:

>Translated_456_residues
MMDALKPKRLMWKIWVLILLIIVTLALMILITVRSSITQFIDEQVYETLRDVDYYLGNTNELENYPQNPIEYDRRQQESR
SVNQLIFLPNGRIIYGSAPLELTNRMYQEALNQSGQVERYQTTIDGEDVYYIIRQNEYDGTIVYQASYAWDAYRRELVST
LYLRISILIGIISLLSLIPALLMSRQLTKPIVEMERAVEKISNRQWETALPLDRQDELGQLAHSIDAMRKELKEQDLAQQ
ALLQNISHDLKTPIMVIRGYAQSIGDGIYPTGDLKGSAEVIDEEASRLEKKVVDLLYVTKLEYFKGQEMKLEPINLDKLM
ELLVARFKLRGQLDWEINGEGGEMLGDGEQIRVAFENVLDNALRYAESCIRIDLHQDDAGVEITIFNDGPPVDESLDLFH
QFSRGKQGKFGLGLFIVQRIVTLHTGEVSLRNTGDGTAVRFYFPRLKSNQPLNSNA
>Mature_456_residues
MMDALKPKRLMWKIWVLILLIIVTLALMILITVRSSITQFIDEQVYETLRDVDYYLGNTNELENYPQNPIEYDRRQQESR
SVNQLIFLPNGRIIYGSAPLELTNRMYQEALNQSGQVERYQTTIDGEDVYYIIRQNEYDGTIVYQASYAWDAYRRELVST
LYLRISILIGIISLLSLIPALLMSRQLTKPIVEMERAVEKISNRQWETALPLDRQDELGQLAHSIDAMRKELKEQDLAQQ
ALLQNISHDLKTPIMVIRGYAQSIGDGIYPTGDLKGSAEVIDEEASRLEKKVVDLLYVTKLEYFKGQEMKLEPINLDKLM
ELLVARFKLRGQLDWEINGEGGEMLGDGEQIRVAFENVLDNALRYAESCIRIDLHQDDAGVEITIFNDGPPVDESLDLFH
QFSRGKQGKFGLGLFIVQRIVTLHTGEVSLRNTGDGTAVRFYFPRLKSNQPLNSNA

Specific function: Member of the two-component regulatory system CssS/CssR required to control the cellular response to secretion stress. Required for the transcription of htrA. Could detect misfolded proteins at the membrane-cell wall interface and then activate CssR by ph

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=261, Percent_Identity=27.2030651340996, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=318, Percent_Identity=25.4716981132075, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082128, Length=302, Percent_Identity=24.1721854304636, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1789808, Length=281, Percent_Identity=25.2669039145907, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790861, Length=282, Percent_Identity=23.4042553191489, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790346, Length=265, Percent_Identity=26.4150943396226, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786912, Length=233, Percent_Identity=27.8969957081545, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=22.2707423580786, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52320; Mature: 52320

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDALKPKRLMWKIWVLILLIIVTLALMILITVRSSITQFIDEQVYETLRDVDYYLGNTN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELENYPQNPIEYDRRQQESRSVNQLIFLPNGRIIYGSAPLELTNRMYQEALNQSGQVERY
HHCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHH
QTTIDGEDVYYIIRQNEYDGTIVYQASYAWDAYRRELVSTLYLRISILIGIISLLSLIPA
EEEECCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMSRQLTKPIVEMERAVEKISNRQWETALPLDRQDELGQLAHSIDAMRKELKEQDLAQQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLQNISHDLKTPIMVIRGYAQSIGDGIYPTGDLKGSAEVIDEEASRLEKKVVDLLYVTK
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEYFKGQEMKLEPINLDKLMELLVARFKLRGQLDWEINGEGGEMLGDGEQIRVAFENVLD
HHHHCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
NALRYAESCIRIDLHQDDAGVEITIFNDGPPVDESLDLFHQFSRGKQGKFGLGLFIVQRI
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
VTLHTGEVSLRNTGDGTAVRFYFPRLKSNQPLNSNA
HHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MMDALKPKRLMWKIWVLILLIIVTLALMILITVRSSITQFIDEQVYETLRDVDYYLGNTN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
ELENYPQNPIEYDRRQQESRSVNQLIFLPNGRIIYGSAPLELTNRMYQEALNQSGQVERY
HHCCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHHHHHHCCCCCCCHH
QTTIDGEDVYYIIRQNEYDGTIVYQASYAWDAYRRELVSTLYLRISILIGIISLLSLIPA
EEEECCCCEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMSRQLTKPIVEMERAVEKISNRQWETALPLDRQDELGQLAHSIDAMRKELKEQDLAQQ
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALLQNISHDLKTPIMVIRGYAQSIGDGIYPTGDLKGSAEVIDEEASRLEKKVVDLLYVTK
HHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEYFKGQEMKLEPINLDKLMELLVARFKLRGQLDWEINGEGGEMLGDGEQIRVAFENVLD
HHHHCCCCCEECCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
NALRYAESCIRIDLHQDDAGVEITIFNDGPPVDESLDLFHQFSRGKQGKFGLGLFIVQRI
HHHHHHHHHHEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHH
VTLHTGEVSLRNTGDGTAVRFYFPRLKSNQPLNSNA
HHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353931; 9639930; 9384377; 11555295 [H]