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The map label for this gene is tcyA [H]

Identifier: 229918159

GI number: 229918159

Start: 2397733

End: 2398491

Strand: Direct

Name: tcyA [H]

Synonym: EAT1b_2439

Alternate gene names: 229918159

Gene position: 2397733-2398491 (Clockwise)

Preceding gene: 229918158

Following gene: 229918160

Centisome position: 79.93

GC content: 48.62

Gene sequence:

>759_bases
ATGAAAAAGACATTAACGAGCGTACTCGCAATCGGAGCTTCAGTCGGGCTTCTTGCAGCTTGCGGTAACGAAACAGACAG
TGGAGCGGACGAAGCGGTCATCACGGTCGGAACAGAGGCGACATACCCACCATTCACGTACAAAGAAAAAGGTGAATTGA
TGGGATATGATATCGACGTCTTGAACGAAGCGGCAGAACGTGCCGGTTACAAAGTGGAATACGAAGCAATGGACTTCAAA
GGACTTGTCCCAGCACTCGATGCTGAGCGTATCGATATGATTGCTAACCAAATGAGCATCACGCCTGAGCGTGAAGAGAA
ATATGCGTTCTCTGACCCTTATGCTGTGTCAGGAGCTCAAGTCATCGTCGGTTCAGACAACAATGACATCCAAGGCATTG
ACGACCTCGATGGAAAAGTAGTCGGATCGACACAAGGTTCGGTCTATGCGCAAATGGCTGAAGAAGCTGGCGCTGAAGTC
AAGTTTTATAAAGGGGCGAACCAAGTCCTTCAAGACCTCGAAGTGGGTCGTTTGGACGCGGCCCTCAACGACCGTCTCTT
CATCTTGACGGAACTTGAAAAAACAGGCTATGACGTCAAAGCAGTCGGTGACATCTTCAATACAAGTGAAGCTGGATTCA
TGACACGTCAAGATAGCGACGTGCTAGACAAGTTGAACGATGCGCTCGCAGAGATGAAAGAAGACGGCACGATGAAAGAA
ATCGGTGAGAAGTACTTCGGTGAGGATATCAGTCAATGA

Upstream 100 bases:

>100_bases
TGATGTTTTTTGTTTGACATGAAGACATCGCGCCAGTATGATGAGGATATTGTTTAAAACCGTGTAATCATATAAGGATT
ATAGGAAAGGCGGAATCATT

Downstream 100 bases:

>100_bases
ATGAACTGATCACGACCGTCATAGATAACCGCTCGGTCTATCTTGAAGCCGTCCAGCTCACGTTGATCGCCAGTGTCCTG
TCGATTCTTCTCGCCTTGGC

Product: extracellular solute-binding protein family 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MKKTLTSVLAIGASVGLLAACGNETDSGADEAVITVGTEATYPPFTYKEKGELMGYDIDVLNEAAERAGYKVEYEAMDFK
GLVPALDAERIDMIANQMSITPEREEKYAFSDPYAVSGAQVIVGSDNNDIQGIDDLDGKVVGSTQGSVYAQMAEEAGAEV
KFYKGANQVLQDLEVGRLDAALNDRLFILTELEKTGYDVKAVGDIFNTSEAGFMTRQDSDVLDKLNDALAEMKEDGTMKE
IGEKYFGEDISQ

Sequences:

>Translated_252_residues
MKKTLTSVLAIGASVGLLAACGNETDSGADEAVITVGTEATYPPFTYKEKGELMGYDIDVLNEAAERAGYKVEYEAMDFK
GLVPALDAERIDMIANQMSITPEREEKYAFSDPYAVSGAQVIVGSDNNDIQGIDDLDGKVVGSTQGSVYAQMAEEAGAEV
KFYKGANQVLQDLEVGRLDAALNDRLFILTELEKTGYDVKAVGDIFNTSEAGFMTRQDSDVLDKLNDALAEMKEDGTMKE
IGEKYFGEDISQ
>Mature_252_residues
MKKTLTSVLAIGASVGLLAACGNETDSGADEAVITVGTEATYPPFTYKEKGELMGYDIDVLNEAAERAGYKVEYEAMDFK
GLVPALDAERIDMIANQMSITPEREEKYAFSDPYAVSGAQVIVGSDNNDIQGIDDLDGKVVGSTQGSVYAQMAEEAGAEV
KFYKGANQVLQDLEVGRLDAALNDRLFILTELEKTGYDVKAVGDIFNTSEAGFMTRQDSDVLDKLNDALAEMKEDGTMKE
IGEKYFGEDISQ

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=265, Percent_Identity=34.7169811320755, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787031, Length=247, Percent_Identity=33.6032388663968, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1788649, Length=259, Percent_Identity=33.976833976834, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=32.9004329004329, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787085, Length=225, Percent_Identity=31.5555555555556, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787088, Length=256, Percent_Identity=30.859375, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27243; Mature: 27243

Theoretical pI: Translated: 3.92; Mature: 3.92

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTLTSVLAIGASVGLLAACGNETDSGADEAVITVGTEATYPPFTYKEKGELMGYDIDV
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
LNEAAERAGYKVEYEAMDFKGLVPALDAERIDMIANQMSITPEREEKYAFSDPYAVSGAQ
HHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCEECCCE
VIVGSDNNDIQGIDDLDGKVVGSTQGSVYAQMAEEAGAEVKFYKGANQVLQDLEVGRLDA
EEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
ALNDRLFILTELEKTGYDVKAVGDIFNTSEAGFMTRQDSDVLDKLNDALAEMKEDGTMKE
HCCCCEEEEEEHHHCCCCCEEHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHH
IGEKYFGEDISQ
HHHHHHCCCCCC
>Mature Secondary Structure
MKKTLTSVLAIGASVGLLAACGNETDSGADEAVITVGTEATYPPFTYKEKGELMGYDIDV
CCHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHH
LNEAAERAGYKVEYEAMDFKGLVPALDAERIDMIANQMSITPEREEKYAFSDPYAVSGAQ
HHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHCCCCCCCCCEECCCE
VIVGSDNNDIQGIDDLDGKVVGSTQGSVYAQMAEEAGAEVKFYKGANQVLQDLEVGRLDA
EEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHH
ALNDRLFILTELEKTGYDVKAVGDIFNTSEAGFMTRQDSDVLDKLNDALAEMKEDGTMKE
HCCCCEEEEEEHHHCCCCCEEHHHHHCCCCCCCEECCCHHHHHHHHHHHHHHHCCCCHHH
IGEKYFGEDISQ
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]