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The map label for this gene is ilvE [H]

Identifier: 229917986

GI number: 229917986

Start: 2218823

End: 2219749

Strand: Direct

Name: ilvE [H]

Synonym: EAT1b_2265

Alternate gene names: 229917986

Gene position: 2218823-2219749 (Clockwise)

Preceding gene: 229917985

Following gene: 229917987

Centisome position: 73.96

GC content: 51.78

Gene sequence:

>927_bases
ATGGAACAGTATGGACAATGGATGTGGAAAGATGGGGCTTGGATTGAGCCGACTGACGCAAACACGAGTATCATGACACA
CGCGATTCACTACGGAAGCGGGTTCTTTGAAGGGATTCGCGCGTACCACACGGAGGAAGGTCCTGCCATCTTCCGGTTGC
GTGAACACTTGGAGCGACTCGTTCGCTCGTGTGCATACTACCACGTCACGCTACCGTATACGGTAGATGAACTCGAACAA
GCCACGATGGAATTGATTGAGCGAAACGGATTTGAAGCATGCTACATCCGTCCGTTCGTCTTCCTTGGAACACCATGGCA
AGCACTTATGCCGACTGCTGAGACGAAGGTACATGTTGCCATCTCGTGTTGGCCGCTCGGTGAATACTTCAACAAGACGG
TCGGGATTCGAGCGAAAGTGGCCTCGTATCGCCGCGTGTCTTCTACCATGATGCCAATGCAAGCGAAAGCGGCATCGAAC
TATATGAACTCACAGTTACTAAAAGGAGAGGCGCTCCGTGACGGATTCGACGAAGCGATTGCCCTTGATATGAACGGAAA
CGTCAGTGAGGCGAGTGTCGCGAACATCTTCCTCGTGAAAGGAAAAACGATTACGACACCGTCCCTTGATTGTTCTGTAC
TCGATGGCATCACACGCCAAACGGTGATGCAATTGGCTCGCGAGGCCGGCTATGAAATCGTCGAGCGCCATATCGGACGC
GACGAGCTCTATGTGGCCGATGAAATCTTCTTGACGGGAACGGCAGCCGAGGTGACGAGCGTCATCGAGGTCGACCATAT
CTCGATTGGTGGAGGTATCCAAACGGTTGCGACAGAAATGTTGACACGCTATCGTGAAGCCGTAACAGGACAACTTCCGG
AACATCGCGATTGGATCACATTTGTGAAATCAGCAGTAAAAGAGTAA

Upstream 100 bases:

>100_bases
GAAAAAACTTCTGAAAAAACGCAACTTGTTGATGACTTACGCTCTCTCTAACGTATAATAGACAGAAAATATAAAAAATG
AGGAATGGGAGAGTTGCATT

Downstream 100 bases:

>100_bases
AGGAGTTTTGCACGATGGGAGTCACAATTGACTTAAATGCGATTCGCGAAAACAAACGCCGGATTGAAGCGCTCGGGCGC
CCCGTGTTTGCGGTCGTGAA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT; Transaminase B [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEQYGQWMWKDGAWIEPTDANTSIMTHAIHYGSGFFEGIRAYHTEEGPAIFRLREHLERLVRSCAYYHVTLPYTVDELEQ
ATMELIERNGFEACYIRPFVFLGTPWQALMPTAETKVHVAISCWPLGEYFNKTVGIRAKVASYRRVSSTMMPMQAKAASN
YMNSQLLKGEALRDGFDEAIALDMNGNVSEASVANIFLVKGKTITTPSLDCSVLDGITRQTVMQLAREAGYEIVERHIGR
DELYVADEIFLTGTAAEVTSVIEVDHISIGGGIQTVATEMLTRYREAVTGQLPEHRDWITFVKSAVKE

Sequences:

>Translated_308_residues
MEQYGQWMWKDGAWIEPTDANTSIMTHAIHYGSGFFEGIRAYHTEEGPAIFRLREHLERLVRSCAYYHVTLPYTVDELEQ
ATMELIERNGFEACYIRPFVFLGTPWQALMPTAETKVHVAISCWPLGEYFNKTVGIRAKVASYRRVSSTMMPMQAKAASN
YMNSQLLKGEALRDGFDEAIALDMNGNVSEASVANIFLVKGKTITTPSLDCSVLDGITRQTVMQLAREAGYEIVERHIGR
DELYVADEIFLTGTAAEVTSVIEVDHISIGGGIQTVATEMLTRYREAVTGQLPEHRDWITFVKSAVKE
>Mature_308_residues
MEQYGQWMWKDGAWIEPTDANTSIMTHAIHYGSGFFEGIRAYHTEEGPAIFRLREHLERLVRSCAYYHVTLPYTVDELEQ
ATMELIERNGFEACYIRPFVFLGTPWQALMPTAETKVHVAISCWPLGEYFNKTVGIRAKVASYRRVSSTMMPMQAKAASN
YMNSQLLKGEALRDGFDEAIALDMNGNVSEASVANIFLVKGKTITTPSLDCSVLDGITRQTVMQLAREAGYEIVERHIGR
DELYVADEIFLTGTAAEVTSVIEVDHISIGGGIQTVATEMLTRYREAVTGQLPEHRDWITFVKSAVKE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010906, Length=311, Percent_Identity=24.7588424437299, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI38176287, Length=311, Percent_Identity=24.7588424437299, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI296010904, Length=311, Percent_Identity=24.7588424437299, Blast_Score=72, Evalue=5e-13,
Organism=Escherichia coli, GI48994963, Length=269, Percent_Identity=40.8921933085502, Blast_Score=224, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17565728, Length=286, Percent_Identity=28.6713286713287, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322002, Length=271, Percent_Identity=27.6752767527675, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34622; Mature: 34622

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00770 AA_TRANSFER_CLASS_4 ; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQYGQWMWKDGAWIEPTDANTSIMTHAIHYGSGFFEGIRAYHTEEGPAIFRLREHLERL
CCCHHCEEECCCCEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
VRSCAYYHVTLPYTVDELEQATMELIERNGFEACYIRPFVFLGTPWQALMPTAETKVHVA
HHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCEEEEE
ISCWPLGEYFNKTVGIRAKVASYRRVSSTMMPMQAKAASNYMNSQLLKGEALRDGFDEAI
EEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCEE
ALDMNGNVSEASVANIFLVKGKTITTPSLDCSVLDGITRQTVMQLAREAGYEIVERHIGR
EEECCCCCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DELYVADEIFLTGTAAEVTSVIEVDHISIGGGIQTVATEMLTRYREAVTGQLPEHRDWIT
CCEEEEEEEEEECCHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FVKSAVKE
HHHHHHCC
>Mature Secondary Structure
MEQYGQWMWKDGAWIEPTDANTSIMTHAIHYGSGFFEGIRAYHTEEGPAIFRLREHLERL
CCCHHCEEECCCCEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
VRSCAYYHVTLPYTVDELEQATMELIERNGFEACYIRPFVFLGTPWQALMPTAETKVHVA
HHHHHEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCHHHHCCCCCCEEEEE
ISCWPLGEYFNKTVGIRAKVASYRRVSSTMMPMQAKAASNYMNSQLLKGEALRDGFDEAI
EEEEECHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHCCCCCEE
ALDMNGNVSEASVANIFLVKGKTITTPSLDCSVLDGITRQTVMQLAREAGYEIVERHIGR
EEECCCCCCCCCEEEEEEEECCEECCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCC
DELYVADEIFLTGTAAEVTSVIEVDHISIGGGIQTVATEMLTRYREAVTGQLPEHRDWIT
CCEEEEEEEEEECCHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHHHH
FVKSAVKE
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]