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The map label for this gene is nagB

Identifier: 229917976

GI number: 229917976

Start: 2209415

End: 2210179

Strand: Direct

Name: nagB

Synonym: EAT1b_2255

Alternate gene names: 229917976

Gene position: 2209415-2210179 (Clockwise)

Preceding gene: 229917975

Following gene: 229917977

Centisome position: 73.65

GC content: 51.9

Gene sequence:

>765_bases
ATGAATGTACTAGTCGCAAAGACGGCGAACGACGCGGAACGCATCGCCTATACGCTAATCAAAGAACGGATTTCAGGGAA
ACATGACTTTGTACTCGGTCTCGCAACCGGTAGCACACCGGTCGGGATGTATCAGATGTTTAAACAGGACGCGCTCGATT
GTAGCCATGTGACGAGCGTCAATTTAGATGAATATATCGGTCTGTCACCAGAGCATCCGCAAAGCTACAACCGTTTTATG
AAAGATCGTTTGTTTGACGAGGTACCGTTTAAACAAAGCCATCTTCCTCAAGGTGATGCGCCAGATCCTCAGGCGGAGGC
GGCTCGTTACGAAGACCTCGTTCGGAAGCTTGGTGTCGACTTACAACTTCTTGGTATCGGGGAAAATGGTCACATCGCAT
TTAATGAACCGGGTACAGCGCTTGATGCGAAGACACACGTGACCGAACTCACCGAATCAACACGAGAGGCAAACCGTCGC
TTTTTTGACCGATTGGAAGACGTCCCGACGCATGCAATCACGATGGGTCTCGATACAATCATGAACGCACGTGAAATCGT
CTTAGTCGCGACAGGGGAGCGGAAAGCTGAAGCGGTACAGCACATGATTGAAAGCGTACCGACCGTCGACTGGCCGGCCA
CAATCCTACAGGCCCATCCAGGTGTGACGGTCGTGCTCGACGGGGCGGCTGCATCGCGCTGTGCGGAATCCTTACAAGCT
CGTGGGCAAGAAGCGGCAACACGATTTTTCACCATTCGGGATTAA

Upstream 100 bases:

>100_bases
AATCGGGGTTTGATGCAGACTTTGTCGTTTGGGATGACACGACACATGTTCAGATGACTTTCGTCAAAGGTCGAATCGTA
TATAAACGGGAGGAACAAAG

Downstream 100 bases:

>100_bases
CGAACCTGCGGAGTCATCTCCGCGGGTTTTTCTGTGTTCAACTGTGGGAAAATGTAAAGAAACGAATTTGTCATCAAAAC
GTTTTCGACAAATTTACCGC

Product: glucosamine-6-phosphate deaminase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNVLVAKTANDAERIAYTLIKERISGKHDFVLGLATGSTPVGMYQMFKQDALDCSHVTSVNLDEYIGLSPEHPQSYNRFM
KDRLFDEVPFKQSHLPQGDAPDPQAEAARYEDLVRKLGVDLQLLGIGENGHIAFNEPGTALDAKTHVTELTESTREANRR
FFDRLEDVPTHAITMGLDTIMNAREIVLVATGERKAEAVQHMIESVPTVDWPATILQAHPGVTVVLDGAAASRCAESLQA
RGQEAATRFFTIRD

Sequences:

>Translated_254_residues
MNVLVAKTANDAERIAYTLIKERISGKHDFVLGLATGSTPVGMYQMFKQDALDCSHVTSVNLDEYIGLSPEHPQSYNRFM
KDRLFDEVPFKQSHLPQGDAPDPQAEAARYEDLVRKLGVDLQLLGIGENGHIAFNEPGTALDAKTHVTELTESTREANRR
FFDRLEDVPTHAITMGLDTIMNAREIVLVATGERKAEAVQHMIESVPTVDWPATILQAHPGVTVVLDGAAASRCAESLQA
RGQEAATRFFTIRD
>Mature_254_residues
MNVLVAKTANDAERIAYTLIKERISGKHDFVLGLATGSTPVGMYQMFKQDALDCSHVTSVNLDEYIGLSPEHPQSYNRFM
KDRLFDEVPFKQSHLPQGDAPDPQAEAARYEDLVRKLGVDLQLLGIGENGHIAFNEPGTALDAKTHVTELTESTREANRR
FFDRLEDVPTHAITMGLDTIMNAREIVLVATGERKAEAVQHMIESVPTVDWPATILQAHPGVTVVLDGAAASRCAESLQA
RGQEAATRFFTIRD

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=240, Percent_Identity=37.9166666666667, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI19923881, Length=224, Percent_Identity=37.9464285714286, Blast_Score=153, Evalue=1e-37,
Organism=Escherichia coli, GI1786893, Length=206, Percent_Identity=41.2621359223301, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1789530, Length=225, Percent_Identity=30.2222222222222, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI48994958, Length=256, Percent_Identity=25, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17554876, Length=229, Percent_Identity=37.117903930131, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24581960, Length=240, Percent_Identity=37.5, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI19920764, Length=240, Percent_Identity=37.5, Blast_Score=165, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_EXISA (C4L2C5)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002886622.1
- ProteinModelPortal:   C4L2C5
- GeneID:   7870842
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_2255
- OMA:   HLSITMG
- ProtClustDB:   PRK00443
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 28029; Mature: 28029

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 63-63 ACT_SITE 129-129 ACT_SITE 131-131 ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVAKTANDAERIAYTLIKERISGKHDFVLGLATGSTPVGMYQMFKQDALDCSHVTSV
CCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCC
NLDEYIGLSPEHPQSYNRFMKDRLFDEVPFKQSHLPQGDAPDPQAEAARYEDLVRKLGVD
CHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
LQLLGIGENGHIAFNEPGTALDAKTHVTELTESTREANRRFFDRLEDVPTHAITMGLDTI
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MNAREIVLVATGERKAEAVQHMIESVPTVDWPATILQAHPGVTVVLDGAAASRCAESLQA
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHH
RGQEAATRFFTIRD
CCHHHHHHEEEECC
>Mature Secondary Structure
MNVLVAKTANDAERIAYTLIKERISGKHDFVLGLATGSTPVGMYQMFKQDALDCSHVTSV
CCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHCCC
NLDEYIGLSPEHPQSYNRFMKDRLFDEVPFKQSHLPQGDAPDPQAEAARYEDLVRKLGVD
CHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCC
LQLLGIGENGHIAFNEPGTALDAKTHVTELTESTREANRRFFDRLEDVPTHAITMGLDTI
EEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
MNAREIVLVATGERKAEAVQHMIESVPTVDWPATILQAHPGVTVVLDGAAASRCAESLQA
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHH
RGQEAATRFFTIRD
CCHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA