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The map label for this gene is clpB [H]

Identifier: 229917967

GI number: 229917967

Start: 2199280

End: 2201871

Strand: Direct

Name: clpB [H]

Synonym: EAT1b_2246

Alternate gene names: 229917967

Gene position: 2199280-2201871 (Clockwise)

Preceding gene: 229917966

Following gene: 229917971

Centisome position: 73.31

GC content: 52.55

Gene sequence:

>2592_bases
ATGGACTTTAATCGTTTTACAGATAAAGCACATGAATGCATTGTCAGTTCACAGGCGTTAGCGAAACAATATCATCATAG
TGAAATCAATGAGTGGCACGTCTTGGCGTCCATGATTTCGCAGACGGACGGGATCGCGCCGTTGCTGTTTCAAAAAATGG
AAATCAACGTGTCTGAGATTGAAGGCGAAGTCGCGCTTTCTTTAAGAAATGCACCAAAACTTCAAAATCCGACGACACCG
ATGATTTCGGCTTCTTTGCTGAACGTTCTAACGACGGCGGAGCGGGAAGCGACAGGGATGAACGATGAATACGTATCGGT
CGAACACATTTTACTCGGCATCTTGTTGAACAGTAGTCAAGCACAGGCGATGCTCGAGCGAAAAGGGATCACAGAAGACC
GTCTCCGTGAGGCGCTCGTCGCCGTACGAGGCAATCGTCGGATTACTTCGAAGTCGCCTGAAGCGACCTATGACGTCTTG
ACGAAATACGGACGAGACCTCATTCAAGAAGTGAAGACTGGGAAAATCGATCCTGTCATCGGACGTGACAGTGAGATTCG
TCGTGTCATCCGGATTTTGAGTCGGAAAACGAAAAACAACCCTGTCCTCATCGGGGAACCGGGTGTCGGGAAGACGGCGA
TTGTCGAAGGGCTCGCCCAGCGTATCGTTCGTGGAGATGTGCCGGAAAGTTTGAAAGACAAAACCATCTTCAGTCTCGAT
ATGAGTTCCCTCGTTGCCGGTGCGAAATATCGTGGGGAGTTCGAGGAACGTCTACAGGCCGTCTTGTCCGAAGTGAAAGA
AGCGGAAGGCACCATCATCCTCTTCATCGATGAACTGCACACGATTGTCGGGGCAGGGAAGTCGGAGGGGTCGATGGACG
CCGGGAACATGTTGAAACCGATGCTCGCCCGAGGCGAACTGCATTGTATCGGTGCGACGACACTTGACGAGTATCGTCAA
TATATCGAGAAGGACCCGGCCCTCGAGCGGCGTTTCCAACAAGTACTTGTCGCTGAACCGGATGTCGAGGATACGGTCTC
GATCTTGCGCGGTTTAAAAGAACGCTTCGAGATTCACCACGGGGTACGCATCCATGACAACGCGCTCGTCGCAGCGGCTG
TATTATCCAATCGCTATATCACCGACCGTTTCATGCCGGACAAGGCAATCGATCTCGTCGACGAGTCGTGTGCAATGATT
CGGACGGAGATGGAGTCGATGCCGGCTGAGCTCGATGAATTGGTTCGTCGGGTCATGCAGCTTGAAATCGAAGAGGCGGC
GCTTAAGAAAGAGAGCGATGCCGCTTCCATGAAGCGACTTGAGGCGCTTCAACAGGAATTGGCATCACTTCGTGAACAGT
CTGACTCGTTACGTCTCCGCTGGGAGAATGAGAAGGAGTCGACGCACCGCGTACAACAAATCCGCGGAGACCTCGAGAAA
GTACGCCTCGAGTTACAAGAGGCAGAGAGTCGTTACGATTTGAATCGGGCCTCGGAACTGAAATACGGACGAATCCCAGA
GCTTGAGCGAGAACTCGAAGAAGCGGAACAGTTGGCGAGTTCGGTGACACGTGAACTCGTGCGTGAAGAGGTGACGGAAG
AAGAAATCGCCGAGGTCGTCTCGAAATGGACGGGCATCCCAGTGACGAAACTGACAGAAGGAGAGCGGGATAAGCTCCTC
AATCTAGAAACGGTCTTGCATAAACGCGTGTTCGGACAAGATGATGCGATTCGTCTCGTCAGCGATGCGGTCATTCGGGC
GCGGGCTGGCATCAAAGATCCGAATCGTCCGATCGGCTCATTCCTCTTCCTCGGACCGACGGGGGTCGGGAAGACAGAAC
TCGGGAAGGCGCTTGCGGAAGCGATGTTTGACTCGGAAGATCATATCGTTCGACTCGATATGAGTGAATATATGGAGAAA
CATGCCGTATCACGTCTCGTTGGTGCCCCTCCAGGCTATATCGGATATGAGGAAGGTGGCCAGTTGACGGAAGCGGTTCG
CCGTAACCCGTACTCGGTCCTCTTACTCGATGAGGTCGAGAAAGCGCATCCGGACGTCTTCAACATCTTGTTGCAACTGC
TCGATGATGGTCGATTGACCGATTCGCACGGGCGCATCGTCGATTTTAAAAATACAATCGTCATCATGACGTCGAACATC
GGGGCGGACATTTTGCTCGAGGCAGCGAGAGATGGTGTGATTGATGAAGATGAAGAGCGTCAAGTGCTCGACAAGCTCCG
TCGTCATTTCCGTCCGGAATTCTTGAACCGGGTCGATGAAACGATTCTGTTCCATCCACTCACGAAAGATCAAATCGGTC
AAATCGTCGAGAAAGCGGTCGGACGGATGAGCGAACGGCTCACGACACGCTCGATTCATATCGAGATCACCGACGCAGCG
AAAACGTTCGTTGCCGATGAAGCGTATGAACCTCAATTCGGTGCGCGACCGATCAACCGTTACGTACAACGGACGATTGA
GACGAAACTTGCCCGTAGCATCCTCGCTGGAGATGTCGAGGATGGGCAAACGGTCATCATCGATGTCGAGAACGGGGCGT
TGACGTTACGCCCGTCGACAGTCAATGCATAA

Upstream 100 bases:

>100_bases
TCGGACTTATTCGTCCAACTCGAGGTCGACCTGCCTCTTGATGAGACGTACCGTTCTGTTTATGAGCAAATCGAACGTTA
ATGGATAAGGAGGAATCGTG

Downstream 100 bases:

>100_bases
AGAAAGAGGAATCCAAAATCGGATTCCTCTTTTATGTATGCCGATGTTTTCTTCTCAAAATCAAATAACTGATGCCTACA
AAAATGAGATAGAGTACCGT

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MDFNRFTDKAHECIVSSQALAKQYHHSEINEWHVLASMISQTDGIAPLLFQKMEINVSEIEGEVALSLRNAPKLQNPTTP
MISASLLNVLTTAEREATGMNDEYVSVEHILLGILLNSSQAQAMLERKGITEDRLREALVAVRGNRRITSKSPEATYDVL
TKYGRDLIQEVKTGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPESLKDKTIFSLD
MSSLVAGAKYRGEFEERLQAVLSEVKEAEGTIILFIDELHTIVGAGKSEGSMDAGNMLKPMLARGELHCIGATTLDEYRQ
YIEKDPALERRFQQVLVAEPDVEDTVSILRGLKERFEIHHGVRIHDNALVAAAVLSNRYITDRFMPDKAIDLVDESCAMI
RTEMESMPAELDELVRRVMQLEIEEAALKKESDAASMKRLEALQQELASLREQSDSLRLRWENEKESTHRVQQIRGDLEK
VRLELQEAESRYDLNRASELKYGRIPELERELEEAEQLASSVTRELVREEVTEEEIAEVVSKWTGIPVTKLTEGERDKLL
NLETVLHKRVFGQDDAIRLVSDAVIRARAGIKDPNRPIGSFLFLGPTGVGKTELGKALAEAMFDSEDHIVRLDMSEYMEK
HAVSRLVGAPPGYIGYEEGGQLTEAVRRNPYSVLLLDEVEKAHPDVFNILLQLLDDGRLTDSHGRIVDFKNTIVIMTSNI
GADILLEAARDGVIDEDEERQVLDKLRRHFRPEFLNRVDETILFHPLTKDQIGQIVEKAVGRMSERLTTRSIHIEITDAA
KTFVADEAYEPQFGARPINRYVQRTIETKLARSILAGDVEDGQTVIIDVENGALTLRPSTVNA

Sequences:

>Translated_863_residues
MDFNRFTDKAHECIVSSQALAKQYHHSEINEWHVLASMISQTDGIAPLLFQKMEINVSEIEGEVALSLRNAPKLQNPTTP
MISASLLNVLTTAEREATGMNDEYVSVEHILLGILLNSSQAQAMLERKGITEDRLREALVAVRGNRRITSKSPEATYDVL
TKYGRDLIQEVKTGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPESLKDKTIFSLD
MSSLVAGAKYRGEFEERLQAVLSEVKEAEGTIILFIDELHTIVGAGKSEGSMDAGNMLKPMLARGELHCIGATTLDEYRQ
YIEKDPALERRFQQVLVAEPDVEDTVSILRGLKERFEIHHGVRIHDNALVAAAVLSNRYITDRFMPDKAIDLVDESCAMI
RTEMESMPAELDELVRRVMQLEIEEAALKKESDAASMKRLEALQQELASLREQSDSLRLRWENEKESTHRVQQIRGDLEK
VRLELQEAESRYDLNRASELKYGRIPELERELEEAEQLASSVTRELVREEVTEEEIAEVVSKWTGIPVTKLTEGERDKLL
NLETVLHKRVFGQDDAIRLVSDAVIRARAGIKDPNRPIGSFLFLGPTGVGKTELGKALAEAMFDSEDHIVRLDMSEYMEK
HAVSRLVGAPPGYIGYEEGGQLTEAVRRNPYSVLLLDEVEKAHPDVFNILLQLLDDGRLTDSHGRIVDFKNTIVIMTSNI
GADILLEAARDGVIDEDEERQVLDKLRRHFRPEFLNRVDETILFHPLTKDQIGQIVEKAVGRMSERLTTRSIHIEITDAA
KTFVADEAYEPQFGARPINRYVQRTIETKLARSILAGDVEDGQTVIIDVENGALTLRPSTVNA
>Mature_863_residues
MDFNRFTDKAHECIVSSQALAKQYHHSEINEWHVLASMISQTDGIAPLLFQKMEINVSEIEGEVALSLRNAPKLQNPTTP
MISASLLNVLTTAEREATGMNDEYVSVEHILLGILLNSSQAQAMLERKGITEDRLREALVAVRGNRRITSKSPEATYDVL
TKYGRDLIQEVKTGKIDPVIGRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPESLKDKTIFSLD
MSSLVAGAKYRGEFEERLQAVLSEVKEAEGTIILFIDELHTIVGAGKSEGSMDAGNMLKPMLARGELHCIGATTLDEYRQ
YIEKDPALERRFQQVLVAEPDVEDTVSILRGLKERFEIHHGVRIHDNALVAAAVLSNRYITDRFMPDKAIDLVDESCAMI
RTEMESMPAELDELVRRVMQLEIEEAALKKESDAASMKRLEALQQELASLREQSDSLRLRWENEKESTHRVQQIRGDLEK
VRLELQEAESRYDLNRASELKYGRIPELERELEEAEQLASSVTRELVREEVTEEEIAEVVSKWTGIPVTKLTEGERDKLL
NLETVLHKRVFGQDDAIRLVSDAVIRARAGIKDPNRPIGSFLFLGPTGVGKTELGKALAEAMFDSEDHIVRLDMSEYMEK
HAVSRLVGAPPGYIGYEEGGQLTEAVRRNPYSVLLLDEVEKAHPDVFNILLQLLDDGRLTDSHGRIVDFKNTIVIMTSNI
GADILLEAARDGVIDEDEERQVLDKLRRHFRPEFLNRVDETILFHPLTKDQIGQIVEKAVGRMSERLTTRSIHIEITDAA
KTFVADEAYEPQFGARPINRYVQRTIETKLARSILAGDVEDGQTVIIDVENGALTLRPSTVNA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=318, Percent_Identity=37.7358490566038, Blast_Score=207, Evalue=4e-53,
Organism=Escherichia coli, GI1788943, Length=851, Percent_Identity=53.3490011750881, Blast_Score=912, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=332, Percent_Identity=43.3734939759036, Blast_Score=285, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=703, Percent_Identity=52.9160739687055, Blast_Score=769, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=867, Percent_Identity=42.4452133794694, Blast_Score=678, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 96948; Mature: 96948

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNRFTDKAHECIVSSQALAKQYHHSEINEWHVLASMISQTDGIAPLLFQKMEINVSEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
EGEVALSLRNAPKLQNPTTPMISASLLNVLTTAEREATGMNDEYVSVEHILLGILLNSSQ
CCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
AQAMLERKGITEDRLREALVAVRGNRRITSKSPEATYDVLTKYGRDLIQEVKTGKIDPVI
HHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPESLKDKTIFSLD
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEC
MSSLVAGAKYRGEFEERLQAVLSEVKEAEGTIILFIDELHTIVGAGKSEGSMDAGNMLKP
HHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHH
MLARGELHCIGATTLDEYRQYIEKDPALERRFQQVLVAEPDVEDTVSILRGLKERFEIHH
HHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GVRIHDNALVAAAVLSNRYITDRFMPDKAIDLVDESCAMIRTEMESMPAELDELVRRVMQ
CEEEECCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LEIEEAALKKESDAASMKRLEALQQELASLREQSDSLRLRWENEKESTHRVQQIRGDLEK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
VRLELQEAESRYDLNRASELKYGRIPELERELEEAEQLASSVTRELVREEVTEEEIAEVV
HHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKWTGIPVTKLTEGERDKLLNLETVLHKRVFGQDDAIRLVSDAVIRARAGIKDPNRPIGS
HHHCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELGKALAEAMFDSEDHIVRLDMSEYMEKHAVSRLVGAPPGYIGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
QLTEAVRRNPYSVLLLDEVEKAHPDVFNILLQLLDDGRLTDSHGRIVDFKNTIVIMTSNI
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCC
GADILLEAARDGVIDEDEERQVLDKLRRHFRPEFLNRVDETILFHPLTKDQIGQIVEKAV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHH
GRMSERLTTRSIHIEITDAAKTFVADEAYEPQFGARPINRYVQRTIETKLARSILAGDVE
HHHHHHHHHEEEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DGQTVIIDVENGALTLRPSTVNA
CCCEEEEEECCCEEEECCCCCCC
>Mature Secondary Structure
MDFNRFTDKAHECIVSSQALAKQYHHSEINEWHVLASMISQTDGIAPLLFQKMEINVSEI
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
EGEVALSLRNAPKLQNPTTPMISASLLNVLTTAEREATGMNDEYVSVEHILLGILLNSSQ
CCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHH
AQAMLERKGITEDRLREALVAVRGNRRITSKSPEATYDVLTKYGRDLIQEVKTGKIDPVI
HHHHHHHCCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
GRDSEIRRVIRILSRKTKNNPVLIGEPGVGKTAIVEGLAQRIVRGDVPESLKDKTIFSLD
CCCHHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCEEEEEC
MSSLVAGAKYRGEFEERLQAVLSEVKEAEGTIILFIDELHTIVGAGKSEGSMDAGNMLKP
HHHHHHCHHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCHHHHHH
MLARGELHCIGATTLDEYRQYIEKDPALERRFQQVLVAEPDVEDTVSILRGLKERFEIHH
HHHCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHC
GVRIHDNALVAAAVLSNRYITDRFMPDKAIDLVDESCAMIRTEMESMPAELDELVRRVMQ
CEEEECCHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LEIEEAALKKESDAASMKRLEALQQELASLREQSDSLRLRWENEKESTHRVQQIRGDLEK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHH
VRLELQEAESRYDLNRASELKYGRIPELERELEEAEQLASSVTRELVREEVTEEEIAEVV
HHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SKWTGIPVTKLTEGERDKLLNLETVLHKRVFGQDDAIRLVSDAVIRARAGIKDPNRPIGS
HHHCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELGKALAEAMFDSEDHIVRLDMSEYMEKHAVSRLVGAPPGYIGYEEGG
EEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
QLTEAVRRNPYSVLLLDEVEKAHPDVFNILLQLLDDGRLTDSHGRIVDFKNTIVIMTSNI
HHHHHHHCCCCEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEECCC
GADILLEAARDGVIDEDEERQVLDKLRRHFRPEFLNRVDETILFHPLTKDQIGQIVEKAV
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHEECCCCHHHHHHHHHHHH
GRMSERLTTRSIHIEITDAAKTFVADEAYEPQFGARPINRYVQRTIETKLARSILAGDVE
HHHHHHHHHEEEEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DGQTVIIDVENGALTLRPSTVNA
CCCEEEEEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]