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The map label for this gene is lacF [H]

Identifier: 229917903

GI number: 229917903

Start: 2118408

End: 2119307

Strand: Direct

Name: lacF [H]

Synonym: EAT1b_2181

Alternate gene names: 229917903

Gene position: 2118408-2119307 (Clockwise)

Preceding gene: 229917902

Following gene: 229917904

Centisome position: 70.62

GC content: 47.67

Gene sequence:

>900_bases
GTGAAAAAACTAGATCGTTACGGCTATCTGTTCATCGCCCCGTTCTGGGTCGTCTTCTTGACATTCAGCATCTATCCGGT
CCTCTTAACGCTTTATTATAGCTTCACGAACTATACGGGGAGCCCGGGTGAAGAGGTCGTCGGGTTTGCGAACTACACAC
GGTTGTTGACGGATACGTACTTTATCGAGGCGTTTTTCAACACCATCAAAATTTGGGGAATTAACTTCGTCTTGCAGATT
GGATTGGCGCTTCTCTTGGCACTCATCTTCTCTGATCTTCGTTTGAAGTTAAAAGGACTCTCGTTCTTCCGCTCGTTCTT
CTATCTTCCAAACTTGATCACAATCAGCTCGGTCGCGCTCTTGTTCGGTATTTTGCTCGACTGGCAGCACGGTTCACTCA
ACATGGTGTTGATGAAAATCGGCTTGATTTCGGATCCAATCAACTGGTTGGCAGAACCGGTGACGGCACAGTTATCCGTT
TCACTCATTTTGACATGGATGTGGTTCGGTCACTCCTTCATCATCGTCATGGCGGGTGTATCAGGAATCTCGACAGATTA
TTTCGAAGCCGCTCACATCGACGGTGCAAACCGTTGGCAGATGTTTACGAAAGTGACATTGCCGCTCTTAAAACCAATTC
TTCTCTACATCATGATCACATCACTCATCGGTGGACTTCAATTGTTCGACCTTCCGATGTTGATCACCGATGGAATCGGT
TCACCAGACGGTTCATTGAACACGATGGTGCTTTACTTGTACAACCAAGCCTTCAAGTACAACAACTATGGCTATGCGTC
AGCGGTCGCTTACGGCCTATTCTTGATCACGCTGATCTTCTCGGCGGTCGTCTTCAAGGGTATGTTCCGTAAAGAGCGCG
CGACACAGAAGGGGGCGTAA

Upstream 100 bases:

>100_bases
AAACAAGCGAAGAACGCTTATCCAGACATCGTTGTAGACTGACGATGACCAACACGAGGGGGGAGCTCCCCCCTCGGTTC
TTATGGAAGGAGAAATACAC

Downstream 100 bases:

>100_bases
GCAATGGAACAAAATGCGGAATCACGGCGAATGCTTCCAGATAGCGAAGCGCAGCCGAAACGGAAAGAGGAGCGTCCGCT
TAAAGTGGCGCCGCCTTCTA

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKKLDRYGYLFIAPFWVVFLTFSIYPVLLTLYYSFTNYTGSPGEEVVGFANYTRLLTDTYFIEAFFNTIKIWGINFVLQI
GLALLLALIFSDLRLKLKGLSFFRSFFYLPNLITISSVALLFGILLDWQHGSLNMVLMKIGLISDPINWLAEPVTAQLSV
SLILTWMWFGHSFIIVMAGVSGISTDYFEAAHIDGANRWQMFTKVTLPLLKPILLYIMITSLIGGLQLFDLPMLITDGIG
SPDGSLNTMVLYLYNQAFKYNNYGYASAVAYGLFLITLIFSAVVFKGMFRKERATQKGA

Sequences:

>Translated_299_residues
MKKLDRYGYLFIAPFWVVFLTFSIYPVLLTLYYSFTNYTGSPGEEVVGFANYTRLLTDTYFIEAFFNTIKIWGINFVLQI
GLALLLALIFSDLRLKLKGLSFFRSFFYLPNLITISSVALLFGILLDWQHGSLNMVLMKIGLISDPINWLAEPVTAQLSV
SLILTWMWFGHSFIIVMAGVSGISTDYFEAAHIDGANRWQMFTKVTLPLLKPILLYIMITSLIGGLQLFDLPMLITDGIG
SPDGSLNTMVLYLYNQAFKYNNYGYASAVAYGLFLITLIFSAVVFKGMFRKERATQKGA
>Mature_299_residues
MKKLDRYGYLFIAPFWVVFLTFSIYPVLLTLYYSFTNYTGSPGEEVVGFANYTRLLTDTYFIEAFFNTIKIWGINFVLQI
GLALLLALIFSDLRLKLKGLSFFRSFFYLPNLITISSVALLFGILLDWQHGSLNMVLMKIGLISDPINWLAEPVTAQLSV
SLILTWMWFGHSFIIVMAGVSGISTDYFEAAHIDGANRWQMFTKVTLPLLKPILLYIMITSLIGGLQLFDLPMLITDGIG
SPDGSLNTMVLYLYNQAFKYNNYGYASAVAYGLFLITLIFSAVVFKGMFRKERATQKGA

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790465, Length=266, Percent_Identity=29.6992481203008, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787570, Length=284, Percent_Identity=26.056338028169, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1789861, Length=292, Percent_Identity=25, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33825; Mature: 33825

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLDRYGYLFIAPFWVVFLTFSIYPVLLTLYYSFTNYTGSPGEEVVGFANYTRLLTDTY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FIEAFFNTIKIWGINFVLQIGLALLLALIFSDLRLKLKGLSFFRSFFYLPNLITISSVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LFGILLDWQHGSLNMVLMKIGLISDPINWLAEPVTAQLSVSLILTWMWFGHSFIIVMAGV
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECC
SGISTDYFEAAHIDGANRWQMFTKVTLPLLKPILLYIMITSLIGGLQLFDLPMLITDGIG
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
SPDGSLNTMVLYLYNQAFKYNNYGYASAVAYGLFLITLIFSAVVFKGMFRKERATQKGA
CCCCCHHHEEHEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLDRYGYLFIAPFWVVFLTFSIYPVLLTLYYSFTNYTGSPGEEVVGFANYTRLLTDTY
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
FIEAFFNTIKIWGINFVLQIGLALLLALIFSDLRLKLKGLSFFRSFFYLPNLITISSVAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LFGILLDWQHGSLNMVLMKIGLISDPINWLAEPVTAQLSVSLILTWMWFGHSFIIVMAGV
HHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEECC
SGISTDYFEAAHIDGANRWQMFTKVTLPLLKPILLYIMITSLIGGLQLFDLPMLITDGIG
CCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
SPDGSLNTMVLYLYNQAFKYNNYGYASAVAYGLFLITLIFSAVVFKGMFRKERATQKGA
CCCCCHHHEEHEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]