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The map label for this gene is mcrA [C]

Identifier: 229917846

GI number: 229917846

Start: 2073393

End: 2074433

Strand: Direct

Name: mcrA [C]

Synonym: EAT1b_2124

Alternate gene names: 229917846

Gene position: 2073393-2074433 (Clockwise)

Preceding gene: 229917844

Following gene: 229917847

Centisome position: 69.12

GC content: 37.18

Gene sequence:

>1041_bases
ATGGCAACACCGATTACAATTTTTAAAAATAATCAGAGACTAAGGGAAGAGTCGAATATTCAATTAGCAGCAACTTTCTT
CTCAGACCTTTTAAATTTAAATAAGTCGAAATGCTTTAACGCGATTGAGAGAGGCTATACATATAACATTCCATTTTTTG
ACCAAGAGGATGAGTATCGATTTATTGCTCCAAAGGATATTGCATTAAAGAGAAGGATTGAATTAGAAGAGAGAGACCAT
CATAATGCCCGTCGGATTTGGCTCATCCCAGCAAATCCGAATGAATATGACTTAGAGAGTGCTTTTGGTCGATATGATTT
TTTACATTGGAGACGGTCCTTCAACTATGAAAATGGGGACATCCTATTTATCTATCTGTCTGGCTATATTCAGAAAGTAC
GTTATAAAGTTGAAGTAATTGAAGGGGTTGTGCGCTCTTCAGATGTGAATTACGATCTTACATTTCGGAAAAGTGAAGAT
CAATTTGAGGATTCTAAAGAATTGGACTGGTCTCGCATTCGTTTGATAGACGAAGTAGACACTGACCTATTGTCATTAGA
ATATTTGCGAGAATATGGGTTGAAAGGAAATATTCAAGGGTCGATGAAGTTGATGGGAGAATTGAAAGACTACATCACTT
CGTTTTTCGACAAGGATTTAACAGCTGAGTATTATGCAGACGAAGTAGCAGAGTCATTAGAAGAAGGAAAACGAAAAGTC
GTTACAGTTAATACGTATGAACGTAATCCGCTCGCACGTAAAAGATGCATGGAGTACTACGGGGTGAGCTGTCAGGTTTG
CCAAATCAACTTTGAAAAAATATATGGTGAAGTCGGTAGAGATTTTATTCACGTCCATCACATTAAACCACTTCATGAGA
TTGGACAGAATTATGTGGTGGATCCAATAACTGACTTAAGACCGGTTTGTCCAAATTGCCATGCGATGCTACATCGAAAA
GAAGGTGGCGAGTACTTAACGATTGAACAGTTGAAAAATAGAATAAAATCTAACCCTGGAGGAAATCATGATTATTTATA
A

Upstream 100 bases:

>100_bases
GCAAAGACGAAAAATCAAGCAGTGGCCTCGTGTAGTATAAAGTTACGACGAAACTATATTAATACATGATTTAATTTCTA
ACAGAGAAGGAGAGTTTGAA

Downstream 100 bases:

>100_bases
CGAACCCAAGTACATGTTTGTAGAGCACATCTTAGAAAAAGAAATTAGTTATATCCTTAGTAAAAACATCGAAGAAAAAA
TGAATCGGCGCGTTAGTGCT

Product: HNH endonuclease

Products: NA

Alternate protein names: Restriction Endonuclease-Like Protein; 5-Methylcytosine-Specific Restriction A; Endonuclease; HNH Endonuclease Family Protein; Restriction Endonuclease; Hnh Endonuclease; Endonuclease-Like Protein

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MATPITIFKNNQRLREESNIQLAATFFSDLLNLNKSKCFNAIERGYTYNIPFFDQEDEYRFIAPKDIALKRRIELEERDH
HNARRIWLIPANPNEYDLESAFGRYDFLHWRRSFNYENGDILFIYLSGYIQKVRYKVEVIEGVVRSSDVNYDLTFRKSED
QFEDSKELDWSRIRLIDEVDTDLLSLEYLREYGLKGNIQGSMKLMGELKDYITSFFDKDLTAEYYADEVAESLEEGKRKV
VTVNTYERNPLARKRCMEYYGVSCQVCQINFEKIYGEVGRDFIHVHHIKPLHEIGQNYVVDPITDLRPVCPNCHAMLHRK
EGGEYLTIEQLKNRIKSNPGGNHDYL

Sequences:

>Translated_346_residues
MATPITIFKNNQRLREESNIQLAATFFSDLLNLNKSKCFNAIERGYTYNIPFFDQEDEYRFIAPKDIALKRRIELEERDH
HNARRIWLIPANPNEYDLESAFGRYDFLHWRRSFNYENGDILFIYLSGYIQKVRYKVEVIEGVVRSSDVNYDLTFRKSED
QFEDSKELDWSRIRLIDEVDTDLLSLEYLREYGLKGNIQGSMKLMGELKDYITSFFDKDLTAEYYADEVAESLEEGKRKV
VTVNTYERNPLARKRCMEYYGVSCQVCQINFEKIYGEVGRDFIHVHHIKPLHEIGQNYVVDPITDLRPVCPNCHAMLHRK
EGGEYLTIEQLKNRIKSNPGGNHDYL
>Mature_345_residues
ATPITIFKNNQRLREESNIQLAATFFSDLLNLNKSKCFNAIERGYTYNIPFFDQEDEYRFIAPKDIALKRRIELEERDHH
NARRIWLIPANPNEYDLESAFGRYDFLHWRRSFNYENGDILFIYLSGYIQKVRYKVEVIEGVVRSSDVNYDLTFRKSEDQ
FEDSKELDWSRIRLIDEVDTDLLSLEYLREYGLKGNIQGSMKLMGELKDYITSFFDKDLTAEYYADEVAESLEEGKRKVV
TVNTYERNPLARKRCMEYYGVSCQVCQINFEKIYGEVGRDFIHVHHIKPLHEIGQNYVVDPITDLRPVCPNCHAMLHRKE
GGEYLTIEQLKNRIKSNPGGNHDYL

Specific function: Restriction Of 5-Methyl And 5-Hydroxymethylcytosines At The Specific DNA Sequence C(Me)Cgg. [C]

COG id: COG3183

COG function: function code V; Predicted restriction endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.21.- [C]

Molecular weight: Translated: 40901; Mature: 40769

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPITIFKNNQRLREESNIQLAATFFSDLLNLNKSKCFNAIERGYTYNIPFFDQEDEYR
CCCCEEEEECCHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCEE
FIAPKDIALKRRIELEERDHHNARRIWLIPANPNEYDLESAFGRYDFLHWRRSFNYENGD
EECCCCHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHCHHHHHHHHHHCCCCCCC
ILFIYLSGYIQKVRYKVEVIEGVVRSSDVNYDLTFRKSEDQFEDSKELDWSRIRLIDEVD
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHC
TDLLSLEYLREYGLKGNIQGSMKLMGELKDYITSFFDKDLTAEYYADEVAESLEEGKRKV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEE
VTVNTYERNPLARKRCMEYYGVSCQVCQINFEKIYGEVGRDFIHVHHIKPLHEIGQNYVV
EEEECCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHCHHEEEEECCCHHHHCCCCCCC
DPITDLRPVCPNCHAMLHRKEGGEYLTIEQLKNRIKSNPGGNHDYL
CCHHHCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ATPITIFKNNQRLREESNIQLAATFFSDLLNLNKSKCFNAIERGYTYNIPFFDQEDEYR
CCCEEEEECCHHHHHCCCCEEHHHHHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCEE
FIAPKDIALKRRIELEERDHHNARRIWLIPANPNEYDLESAFGRYDFLHWRRSFNYENGD
EECCCCHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCHHHHHCHHHHHHHHHHCCCCCCC
ILFIYLSGYIQKVRYKVEVIEGVVRSSDVNYDLTFRKSEDQFEDSKELDWSRIRLIDEVD
EEEEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHCCCCCCCCHHHHHHHHHHC
TDLLSLEYLREYGLKGNIQGSMKLMGELKDYITSFFDKDLTAEYYADEVAESLEEGKRKV
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEE
VTVNTYERNPLARKRCMEYYGVSCQVCQINFEKIYGEVGRDFIHVHHIKPLHEIGQNYVV
EEEECCCCCHHHHHHHHHHHCCEEEEEEEEHHHHHHHHCHHEEEEECCCHHHHCCCCCCC
DPITDLRPVCPNCHAMLHRKEGGEYLTIEQLKNRIKSNPGGNHDYL
CCHHHCCCCCCCHHHHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA