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The map label for this gene is amyX [H]

Identifier: 229917760

GI number: 229917760

Start: 1991521

End: 1993518

Strand: Direct

Name: amyX [H]

Synonym: EAT1b_2038

Alternate gene names: 229917760

Gene position: 1991521-1993518 (Clockwise)

Preceding gene: 229917757

Following gene: 229917762

Centisome position: 66.39

GC content: 51.75

Gene sequence:

>1998_bases
ATGGAACGTACAATGGATCAACACACACATGTAGACTTAGATCACGTTGCGTATCACGGGAAAGACCTAGGGGTGACGTT
CAAAGGGGAGCAGATTCGACTACGAGTTTGGTCTCCGGTAGCGGAACAGATGTCGGTCAAGTTATATCGAACAGCCCGTG
CCCGGAAGTTTGATCGGCTCGAGCTCGAACCGGCTGAGAAGGGGACGTGGGTGATCGAACTCGATCGTGCCTTGTTTGAA
GGTCAATTTTATGTATTTGAAGCACTGATTGATGGGAAGAAAGTAGAGTCGCTCGATCCGTATGCGAAAGTCGTCGGAGT
GAACGGGAAACGAGGATGTCTGCTTGATCCGGCATCTTTGAACCCGGACCATTGGATCAAAGAACGTCCGCTATTCAATT
CTTCACAAGAGGCGGTCATCTATGAGGCGCACGTACGTGATTTGACGAGTCACCCGGAGAGTGGGGCGACGCACCGTGGG
AAGTTCTTAGGGATGACCGAGACGGGTACGACGACCACGAGCGGTTACCCGACCGGACTTGATTACATCACCTCGCTCGG
AATCACACATCTTCAATTAATGCCGTTCTTTGACTACGGCTCGGTGAATGAGTCACGTGAAGGGGAAGACAACTATAACT
GGGGCTACGATCCGGTTCATTACTTTGCGGTTGAAGGTTCTTACGCCTCGTCGGCAGATGATCCGGCGTCGCGAATTGTC
GAATTGAAGGCAATGATTCAAGCGCTCCACGATCGGGGCATCCGGGTCATCATGGACGTCGTCTTCAATCATACGTACGA
TGCGTTGACAACACCGCTTGGTCAATTCGTCCCGGATTATTACTATCGCTTAAATGAAGACGGGACACTCGCGGATGGTT
CAGCATGTGGGAATGACACGGCCTCTGAGCGTACGATGATGCGTAAGCTCATGGTCGAGTGTGTCACGTACTGGGCGAAA
GAATTCATGATTGACGGGTTCCGTTTTGATTTGATGGGACTCCATGACGTCAAAACGATGAACCAAATTCGTAAGGCGCT
CGATCGTGTCGACCCATCGATTCTCGTCATCGGGGAAGGCTGGGACTTGGATACGCCGCTCCCGGTTCGTAAAAAAGCGA
ATCAACATAATGCGCATAAGATGCCACGCATCGCCCAATTCAATGATGGGATTCGAGACGGTGTCCGCGGAGACGTCTTT
ATCGAAGACTTACCGGGCTGGGTGAGCGGCAACACGGACATGACAGCAGACGTGAAACGTGGGATTGCTGGTGGCATCAC
GAGCCAAAGTTTCGCTGATGAGCCGAACCAGGTCGTCAACTATGTGGAATGTCATGATAATTTGACGCTTTGGGACAAGT
TGTCCGTGACGAATCCGGAAGATGATGAGACGACACGTCGCCGTCGCCACCGTTTGGCGACATCGATTGTGCTCCTGAGT
CAAGGGATTCCATTCCTTCACAGTGGACAGGAGTTCTTCCGGACAAAAGACGGGGATGAGAACAGCTTCAATTCAGGCGA
AGTCGTCAACCGTGTGGACTGGAACCGGGCGGAGCAAGAAGCGCCAAGTGTGGAATATGTGAAAGGTCTCTTGCGTCTGC
GTAAGCAGTATCCGCTCTTCCGACTCGAAAACACGGAACAGATTCGCAAACACTTACGTTTCTTTGATGAGGCAGAAGGT
GTGATTGCATTTGAATTGAGCCGTCATGTGGAAGGATATGTAGAACGACATATCGTATACCACAACGCACTCGAGGAAGC
GGTTGAGGTCAAACTTCCGTCTGGCAAGTTTGAGGTGAACGTAGAGGATCATACCGTTAATTTGACGGCACCACGTCAAG
TCGAGGACCGTCATATGACGGTTGCCCCGCTCTCGACACTTGTCGTGACCGAGCGTCGCCCAGATTACAGCAAGTACGCT
GTGGCCGGTGGTGCCGCACTCGCCGTAATCGGTGGTCTCTGGTATGTATCGAAAAAGCGGAAACGCAAAGAGCAGTAA

Upstream 100 bases:

>100_bases
GACACGATATCGAAAGAATTGTCTTTTGCCATGTTTCATCCATCCTTTGTGTGGTTTTGTTATAATGATAGCAGATTTGA
AGCAGTGAAGGAGTGAAGAG

Downstream 100 bases:

>100_bases
CCAAAAAGGAGACGTTAATCCGTCTCCTTTGGTGTGTCTTCCTCTGATTGAGATGCCTCGAGTTGCATCATTTTTTGAAC
GAGGATGTGTTTTGGCATAT

Product: pullulanase, type I

Products: NA

Alternate protein names: Alpha-dextrin endo-1,6-alpha-glucosidase; Pullulan 6-glucanohydrolase [H]

Number of amino acids: Translated: 665; Mature: 665

Protein sequence:

>665_residues
MERTMDQHTHVDLDHVAYHGKDLGVTFKGEQIRLRVWSPVAEQMSVKLYRTARARKFDRLELEPAEKGTWVIELDRALFE
GQFYVFEALIDGKKVESLDPYAKVVGVNGKRGCLLDPASLNPDHWIKERPLFNSSQEAVIYEAHVRDLTSHPESGATHRG
KFLGMTETGTTTTSGYPTGLDYITSLGITHLQLMPFFDYGSVNESREGEDNYNWGYDPVHYFAVEGSYASSADDPASRIV
ELKAMIQALHDRGIRVIMDVVFNHTYDALTTPLGQFVPDYYYRLNEDGTLADGSACGNDTASERTMMRKLMVECVTYWAK
EFMIDGFRFDLMGLHDVKTMNQIRKALDRVDPSILVIGEGWDLDTPLPVRKKANQHNAHKMPRIAQFNDGIRDGVRGDVF
IEDLPGWVSGNTDMTADVKRGIAGGITSQSFADEPNQVVNYVECHDNLTLWDKLSVTNPEDDETTRRRRHRLATSIVLLS
QGIPFLHSGQEFFRTKDGDENSFNSGEVVNRVDWNRAEQEAPSVEYVKGLLRLRKQYPLFRLENTEQIRKHLRFFDEAEG
VIAFELSRHVEGYVERHIVYHNALEEAVEVKLPSGKFEVNVEDHTVNLTAPRQVEDRHMTVAPLSTLVVTERRPDYSKYA
VAGGAALAVIGGLWYVSKKRKRKEQ

Sequences:

>Translated_665_residues
MERTMDQHTHVDLDHVAYHGKDLGVTFKGEQIRLRVWSPVAEQMSVKLYRTARARKFDRLELEPAEKGTWVIELDRALFE
GQFYVFEALIDGKKVESLDPYAKVVGVNGKRGCLLDPASLNPDHWIKERPLFNSSQEAVIYEAHVRDLTSHPESGATHRG
KFLGMTETGTTTTSGYPTGLDYITSLGITHLQLMPFFDYGSVNESREGEDNYNWGYDPVHYFAVEGSYASSADDPASRIV
ELKAMIQALHDRGIRVIMDVVFNHTYDALTTPLGQFVPDYYYRLNEDGTLADGSACGNDTASERTMMRKLMVECVTYWAK
EFMIDGFRFDLMGLHDVKTMNQIRKALDRVDPSILVIGEGWDLDTPLPVRKKANQHNAHKMPRIAQFNDGIRDGVRGDVF
IEDLPGWVSGNTDMTADVKRGIAGGITSQSFADEPNQVVNYVECHDNLTLWDKLSVTNPEDDETTRRRRHRLATSIVLLS
QGIPFLHSGQEFFRTKDGDENSFNSGEVVNRVDWNRAEQEAPSVEYVKGLLRLRKQYPLFRLENTEQIRKHLRFFDEAEG
VIAFELSRHVEGYVERHIVYHNALEEAVEVKLPSGKFEVNVEDHTVNLTAPRQVEDRHMTVAPLSTLVVTERRPDYSKYA
VAGGAALAVIGGLWYVSKKRKRKEQ
>Mature_665_residues
MERTMDQHTHVDLDHVAYHGKDLGVTFKGEQIRLRVWSPVAEQMSVKLYRTARARKFDRLELEPAEKGTWVIELDRALFE
GQFYVFEALIDGKKVESLDPYAKVVGVNGKRGCLLDPASLNPDHWIKERPLFNSSQEAVIYEAHVRDLTSHPESGATHRG
KFLGMTETGTTTTSGYPTGLDYITSLGITHLQLMPFFDYGSVNESREGEDNYNWGYDPVHYFAVEGSYASSADDPASRIV
ELKAMIQALHDRGIRVIMDVVFNHTYDALTTPLGQFVPDYYYRLNEDGTLADGSACGNDTASERTMMRKLMVECVTYWAK
EFMIDGFRFDLMGLHDVKTMNQIRKALDRVDPSILVIGEGWDLDTPLPVRKKANQHNAHKMPRIAQFNDGIRDGVRGDVF
IEDLPGWVSGNTDMTADVKRGIAGGITSQSFADEPNQVVNYVECHDNLTLWDKLSVTNPEDDETTRRRRHRLATSIVLLS
QGIPFLHSGQEFFRTKDGDENSFNSGEVVNRVDWNRAEQEAPSVEYVKGLLRLRKQYPLFRLENTEQIRKHLRFFDEAEG
VIAFELSRHVEGYVERHIVYHNALEEAVEVKLPSGKFEVNVEDHTVNLTAPRQVEDRHMTVAPLSTLVVTERRPDYSKYA
VAGGAALAVIGGLWYVSKKRKRKEQ

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=537, Percent_Identity=27.7467411545624, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR011840 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.41 [H]

Molecular weight: Translated: 75555; Mature: 75555

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERTMDQHTHVDLDHVAYHGKDLGVTFKGEQIRLRVWSPVAEQMSVKLYRTARARKFDRL
CCCCCCCCCCCCHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCCCC
ELEPAEKGTWVIELDRALFEGQFYVFEALIDGKKVESLDPYAKVVGVNGKRGCLLDPASL
CCCCCCCCCEEEEECHHHHCCCCEEEEEHHCCCCCCCCCCCHHEEECCCCCCCEECCCCC
NPDHWIKERPLFNSSQEAVIYEAHVRDLTSHPESGATHRGKFLGMTETGTTTTSGYPTGL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
DYITSLGITHLQLMPFFDYGSVNESREGEDNYNWGYDPVHYFAVEGSYASSADDPASRIV
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
ELKAMIQALHDRGIRVIMDVVFNHTYDALTTPLGQFVPDYYYRLNEDGTLADGSACGNDT
HHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCHHHHCCHHHEEECCCCCCCCCCCCCCCC
ASERTMMRKLMVECVTYWAKEFMIDGFRFDLMGLHDVKTMNQIRKALDRVDPSILVIGEG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECC
WDLDTPLPVRKKANQHNAHKMPRIAQFNDGIRDGVRGDVFIEDLPGWVSGNTDMTADVKR
CCCCCCCCHHHCCCCCCHHCCCCHHHHCCCHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
GIAGGITSQSFADEPNQVVNYVECHDNLTLWDKLSVTNPEDDETTRRRRHRLATSIVLLS
CCCCCCCCCHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QGIPFLHSGQEFFRTKDGDENSFNSGEVVNRVDWNRAEQEAPSVEYVKGLLRLRKQYPLF
CCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHHHCCEE
RLENTEQIRKHLRFFDEAEGVIAFELSRHVEGYVERHIVYHNALEEAVEVKLPSGKFEVN
EECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEE
VEDHTVNLTAPRQVEDRHMTVAPLSTLVVTERRPDYSKYAVAGGAALAVIGGLWYVSKKR
EECCEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCHHEECCCHHHHHHHHHHHHHHHH
KRKEQ
HCCCC
>Mature Secondary Structure
MERTMDQHTHVDLDHVAYHGKDLGVTFKGEQIRLRVWSPVAEQMSVKLYRTARARKFDRL
CCCCCCCCCCCCHHHHHCCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHHCCCCCC
ELEPAEKGTWVIELDRALFEGQFYVFEALIDGKKVESLDPYAKVVGVNGKRGCLLDPASL
CCCCCCCCCEEEEECHHHHCCCCEEEEEHHCCCCCCCCCCCHHEEECCCCCCCEECCCCC
NPDHWIKERPLFNSSQEAVIYEAHVRDLTSHPESGATHRGKFLGMTETGTTTTSGYPTGL
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
DYITSLGITHLQLMPFFDYGSVNESREGEDNYNWGYDPVHYFAVEGSYASSADDPASRIV
HHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHH
ELKAMIQALHDRGIRVIMDVVFNHTYDALTTPLGQFVPDYYYRLNEDGTLADGSACGNDT
HHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCHHHHCCHHHEEECCCCCCCCCCCCCCCC
ASERTMMRKLMVECVTYWAKEFMIDGFRFDLMGLHDVKTMNQIRKALDRVDPSILVIGEG
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECC
WDLDTPLPVRKKANQHNAHKMPRIAQFNDGIRDGVRGDVFIEDLPGWVSGNTDMTADVKR
CCCCCCCCHHHCCCCCCHHCCCCHHHHCCCHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
GIAGGITSQSFADEPNQVVNYVECHDNLTLWDKLSVTNPEDDETTRRRRHRLATSIVLLS
CCCCCCCCCHHCCCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
QGIPFLHSGQEFFRTKDGDENSFNSGEVVNRVDWNRAEQEAPSVEYVKGLLRLRKQYPLF
CCCCCCCCCHHHHHCCCCCCCCCCCCCCEECCCCCHHHHCCCCHHHHHHHHHHHHHCCEE
RLENTEQIRKHLRFFDEAEGVIAFELSRHVEGYVERHIVYHNALEEAVEVKLPSGKFEVN
EECCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEE
VEDHTVNLTAPRQVEDRHMTVAPLSTLVVTERRPDYSKYAVAGGAALAVIGGLWYVSKKR
EECCEEEECCCCCCCCCCEEEECCEEEEEECCCCCCCHHEECCCHHHHHHHHHHHHHHHH
KRKEQ
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]