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The map label for this gene is yfkM [H]

Identifier: 229917719

GI number: 229917719

Start: 1951333

End: 1951845

Strand: Direct

Name: yfkM [H]

Synonym: EAT1b_1996

Alternate gene names: 229917719

Gene position: 1951333-1951845 (Clockwise)

Preceding gene: 229917718

Following gene: 229917720

Centisome position: 65.05

GC content: 50.1

Gene sequence:

>513_bases
ATGGCAAAAGTAGCAACGCTCATTACGGATATGTTTGAAGATGTGGAATTCACGGGTCCCCGTGACGCATTGGTGGATGC
GGGACATGATGTCGTGACGATCGAGAAGAAAGCGGGTAATACGGTAGAAGGGAAACAAGGGGAGGCGAAAGTGAACATCG
ACCAATCGATTGACGATGTATCGCCAGAAGATTTTGATGCCCTTCTCTTGCCGGGCGGATTCTCACCTGACTTGTTGCGT
GAGGACGAGCGTTTCGTCTCGTTCGCCAAAGCCTTCATGGATGCGAAAAAGCCTGTCTTTGCCATCTGTCACGGACCACA
GCTGTTGATTACGGCGAAGACGTTAGAAGGTCGTGACGCGACCGGCTACAAATCGATTAAAGTCGACATGGAGTATGCCG
GAGCGAAATATGCGGACAAAGAAGTTGTCGTCTGCCAAAACCAGCTCGTGACGAGCCGTCAGCCGGATGATATTCCAGCA
TTCAATCGCGAGATGCTCAACTTATTGAAATAA

Upstream 100 bases:

>100_bases
GTCTCGAAGTTCCGTTGAACGCCACTTGAGATGTTTAACTGATTCATGCCAGTGGAACAGTCCTTATGTGCATAAAACAA
ACAGAGGGAGTGTTTAGAAC

Downstream 100 bases:

>100_bases
CACGTCGACCACGAGATTCGTCTCGTGGTTTTTTTGTGTTTTCGGGAATAGACAAGTAAGGAGGAGATTTAAATGAAAAA
ACGAGCACGTTCACCGAGTT

Product: intracellular protease, PfpI family

Products: NA

Alternate protein names: GSP18 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MAKVATLITDMFEDVEFTGPRDALVDAGHDVVTIEKKAGNTVEGKQGEAKVNIDQSIDDVSPEDFDALLLPGGFSPDLLR
EDERFVSFAKAFMDAKKPVFAICHGPQLLITAKTLEGRDATGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRQPDDIPA
FNREMLNLLK

Sequences:

>Translated_170_residues
MAKVATLITDMFEDVEFTGPRDALVDAGHDVVTIEKKAGNTVEGKQGEAKVNIDQSIDDVSPEDFDALLLPGGFSPDLLR
EDERFVSFAKAFMDAKKPVFAICHGPQLLITAKTLEGRDATGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRQPDDIPA
FNREMLNLLK
>Mature_169_residues
AKVATLITDMFEDVEFTGPRDALVDAGHDVVTIEKKAGNTVEGKQGEAKVNIDQSIDDVSPEDFDALLLPGGFSPDLLRE
DERFVSFAKAFMDAKKPVFAICHGPQLLITAKTLEGRDATGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRQPDDIPAF
NREMLNLLK

Specific function: Functions in the protection against aldehyde-stress, possibly by degrading damaged proteins [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=167, Percent_Identity=63.4730538922156, Blast_Score=221, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 18682; Mature: 18551

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVATLITDMFEDVEFTGPRDALVDAGHDVVTIEKKAGNTVEGKQGEAKVNIDQSIDDV
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCC
SPEDFDALLLPGGFSPDLLREDERFVSFAKAFMDAKKPVFAICHGPQLLITAKTLEGRDA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCC
TGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRQPDDIPAFNREMLNLLK
CCCEEEEEEEECCCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKVATLITDMFEDVEFTGPRDALVDAGHDVVTIEKKAGNTVEGKQGEAKVNIDQSIDDV
CHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCCCC
SPEDFDALLLPGGFSPDLLREDERFVSFAKAFMDAKKPVFAICHGPQLLITAKTLEGRDA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEECCCCCC
TGYKSIKVDMEYAGAKYADKEVVVCQNQLVTSRQPDDIPAFNREMLNLLK
CCCEEEEEEEECCCCCCCCCEEEEECCHHHCCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377; 9298659 [H]