Click here to switch to the map view.

The map label for this gene is thiF [H]

Identifier: 229917642

GI number: 229917642

Start: 1878352

End: 1879362

Strand: Direct

Name: thiF [H]

Synonym: EAT1b_1918

Alternate gene names: 229917642

Gene position: 1878352-1879362 (Clockwise)

Preceding gene: 229917641

Following gene: 229917643

Centisome position: 62.61

GC content: 58.26

Gene sequence:

>1011_bases
ATGACACATGTTCCGGAACGTTACGCGCGGCAGGCCCGGTTCCACGGTATCGGTGGGGACGGGCAAGAACGTCTGATGGT
GAAACGAGTGCTCGTCGTCGGTCTCGGTGCGCTCGGAGCGGCCGTGAGCGATCAACTTGTTCGGGCCGGCGTCTCACTTA
TCCTCGTCGACCGCGACTATGTAGAGGTGACGAACTTACAACGACAGACGCTCTATACGGAACAGGACGCACGTGCCGAG
ACGCCGAAAGCGGTCGCGGCGCTCGAACGCCTGCGGACCATCAACTCAGACGTTGAGTTGGACGCGCAGATCGCCGACGT
ATCGTGGGATTGGCTTCGGCCTTTGCCGCCCGTTGATCTCGTCTTGGACGCGACCGATAACGTCGAGACACGTCTTCTAT
TGAACGATTTCGCACTCGAGCGCGGCATTCCGTTCTTGTTTGCCTCTTGTGTCGGGGCATACGGCATGAACTACACGGTG
ATGCCGGAAGAAGCGCCGTGTCTTCGTTGTCTCATGCGTCATTTGCCGTTAGCCGGACAGACGTGCGCGACAGCGGGGGT
GGTGGCTCCCATCGTGCAACTTGTGGCGAGCCGTCAAGCCGTCGAGGCGTTGAAATATTTGACGGGGCGTCATGAAAAGA
TGGAACGTCGACTGTTGATTGAAGACATGTGGGAGAATCAGTCACAGCGGCTCGATGTCGCACGGCTCGTCGACGTGTCT
TGCCCGTCTTGTCAGACCCATGACTACCCGAGTCTCGTTTCAAGTCGACAGCAGACAGCGCTCCTATGTGGTCGCGATGT
CGTCCAAGTCCGTCGCTCCATCGAAGGGCTTGACCCGATTGCGCATCAACTCGAGCGGGCGGACATCACCGTGAAGCAGA
CCCCGTTCACGTTGGAATGGCGATGGCGGAATCATCGGATGCTCTTGTTCAAAGACGGAAGGGTTCTCGTCCATGGTGTT
GACGATGCGCATGTCGCGGTCGCGATGGTCGATGAATGTCTTGGTACGTAA

Upstream 100 bases:

>100_bases
TGACCGTATACGATATGACGAAAGCGGTCGATAAAGGCATCGTCATCGGGCCGACATACTTGCTTGAGAAACAAGGCGGG
AAGTCAGGGGACTACGTCCG

Downstream 100 bases:

>100_bases
TTCATCGCATCATAGAGCTTGTTTTTCCCTAAAAAAATACAAAATGATACAACAATTTCTGCGACACGTCACCAACGGGA
AGTCGGAACGGGTTACTTCG

Product: UBA/THIF-type NAD/FAD binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTHVPERYARQARFHGIGGDGQERLMVKRVLVVGLGALGAAVSDQLVRAGVSLILVDRDYVEVTNLQRQTLYTEQDARAE
TPKAVAALERLRTINSDVELDAQIADVSWDWLRPLPPVDLVLDATDNVETRLLLNDFALERGIPFLFASCVGAYGMNYTV
MPEEAPCLRCLMRHLPLAGQTCATAGVVAPIVQLVASRQAVEALKYLTGRHEKMERRLLIEDMWENQSQRLDVARLVDVS
CPSCQTHDYPSLVSSRQQTALLCGRDVVQVRRSIEGLDPIAHQLERADITVKQTPFTLEWRWRNHRMLLFKDGRVLVHGV
DDAHVAVAMVDECLGT

Sequences:

>Translated_336_residues
MTHVPERYARQARFHGIGGDGQERLMVKRVLVVGLGALGAAVSDQLVRAGVSLILVDRDYVEVTNLQRQTLYTEQDARAE
TPKAVAALERLRTINSDVELDAQIADVSWDWLRPLPPVDLVLDATDNVETRLLLNDFALERGIPFLFASCVGAYGMNYTV
MPEEAPCLRCLMRHLPLAGQTCATAGVVAPIVQLVASRQAVEALKYLTGRHEKMERRLLIEDMWENQSQRLDVARLVDVS
CPSCQTHDYPSLVSSRQQTALLCGRDVVQVRRSIEGLDPIAHQLERADITVKQTPFTLEWRWRNHRMLLFKDGRVLVHGV
DDAHVAVAMVDECLGT
>Mature_335_residues
THVPERYARQARFHGIGGDGQERLMVKRVLVVGLGALGAAVSDQLVRAGVSLILVDRDYVEVTNLQRQTLYTEQDARAET
PKAVAALERLRTINSDVELDAQIADVSWDWLRPLPPVDLVLDATDNVETRLLLNDFALERGIPFLFASCVGAYGMNYTVM
PEEAPCLRCLMRHLPLAGQTCATAGVVAPIVQLVASRQAVEALKYLTGRHEKMERRLLIEDMWENQSQRLDVARLVDVSC
PSCQTHDYPSLVSSRQQTALLCGRDVVQVRRSIEGLDPIAHQLERADITVKQTPFTLEWRWRNHRMLLFKDGRVLVHGVD
DAHVAVAMVDECLGT

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=223, Percent_Identity=36.7713004484305, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787048, Length=243, Percent_Identity=36.2139917695473, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI87082356, Length=242, Percent_Identity=36.7768595041322, Blast_Score=109, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17540406, Length=265, Percent_Identity=32.0754716981132, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321903, Length=281, Percent_Identity=29.8932384341637, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=24.7058823529412, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582879, Length=264, Percent_Identity=30.6818181818182, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 37597; Mature: 37466

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVPERYARQARFHGIGGDGQERLMVKRVLVVGLGALGAAVSDQLVRAGVSLILVDRDY
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCH
VEVTNLQRQTLYTEQDARAETPKAVAALERLRTINSDVELDAQIADVSWDWLRPLPPVDL
HHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEECEEEECCCHHHHCCCCCCEE
VLDATDNVETRLLLNDFALERGIPFLFASCVGAYGMNYTVMPEEAPCLRCLMRHLPLAGQ
EEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCC
TCATAGVVAPIVQLVASRQAVEALKYLTGRHEKMERRLLIEDMWENQSQRLDVARLVDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCHHHHHCCC
CPSCQTHDYPSLVSSRQQTALLCGRDVVQVRRSIEGLDPIAHQLERADITVKQTPFTLEW
CCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCEEEEE
RWRNHRMLLFKDGRVLVHGVDDAHVAVAMVDECLGT
EECCCEEEEEECCEEEEEECCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
THVPERYARQARFHGIGGDGQERLMVKRVLVVGLGALGAAVSDQLVRAGVSLILVDRDY
CCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCH
VEVTNLQRQTLYTEQDARAETPKAVAALERLRTINSDVELDAQIADVSWDWLRPLPPVDL
HHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEECEEEECCCHHHHCCCCCCEE
VLDATDNVETRLLLNDFALERGIPFLFASCVGAYGMNYTVMPEEAPCLRCLMRHLPLAGQ
EEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCC
TCATAGVVAPIVQLVASRQAVEALKYLTGRHEKMERRLLIEDMWENQSQRLDVARLVDVS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHCCHHHHHCCC
CPSCQTHDYPSLVSSRQQTALLCGRDVVQVRRSIEGLDPIAHQLERADITVKQTPFTLEW
CCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEEECCCEEEEE
RWRNHRMLLFKDGRVLVHGVDDAHVAVAMVDECLGT
EECCCEEEEEECCEEEEEECCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]