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The map label for this gene is ogt [H]

Identifier: 229917513

GI number: 229917513

Start: 1755853

End: 1756335

Strand: Direct

Name: ogt [H]

Synonym: EAT1b_1788

Alternate gene names: 229917513

Gene position: 1755853-1756335 (Clockwise)

Preceding gene: 229917512

Following gene: 229917514

Centisome position: 58.53

GC content: 51.97

Gene sequence:

>483_bases
ATGGGAACGTATAAATTAGACGTGGAGACACCGATTGGCGTCCTAGAAATCATCGGGACGGAGCAGGTCGTTCAATCGAT
CTTATTCGTCGAACGGGAAAGCAAACGAAGTATGGAGCAAGATGTACCAAATGTATTGCGACAGTGCCGGGATGAACTAA
TCGCCTACTTTCGTGGCGAGCGTATGACGTTCACGTTCCCGACTGAGCAAATCGGGACAACGTTTCAGCATGACGTGTGG
GATGCGTTACAAACCATCCCGTATGCGGAGACGGCTTCTTACCGCGAACTTGCCGAACGCGTGAAGCGACCTACGGCTGT
TCGCGCAGTCGGAGCAACGAACGGACGCAACAAACTGAGCATCGTCGTGCCTTGTCATCGCGTCATCGGATCGACTGGGA
AATTGACGGGCTATGCCGGTGGACTTTGGCGAAAAGAATGGCTCTTGCAACATGAAAAACAAGTCGCACATCAACCCCAG
TAA

Upstream 100 bases:

>100_bases
GGGACAGCGAAGAAACCAACGATTGAAGAAGTGAAGCGCTACGGTGAATCGTGGCAAGGTTTTGAAGGATACGCGACATT
CTATCTGTGGAGGTCATTAT

Downstream 100 bases:

>100_bases
AAAAACATTTCCCCCTCCAAATCTGCTACACTAGAAGAGGTCCTATCGTGACGTCGGAATCGACGAAGTTATAGGATGAT
TCCCGTCACTTATGGAGAAA

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MGTYKLDVETPIGVLEIIGTEQVVQSILFVERESKRSMEQDVPNVLRQCRDELIAYFRGERMTFTFPTEQIGTTFQHDVW
DALQTIPYAETASYRELAERVKRPTAVRAVGATNGRNKLSIVVPCHRVIGSTGKLTGYAGGLWRKEWLLQHEKQVAHQPQ

Sequences:

>Translated_160_residues
MGTYKLDVETPIGVLEIIGTEQVVQSILFVERESKRSMEQDVPNVLRQCRDELIAYFRGERMTFTFPTEQIGTTFQHDVW
DALQTIPYAETASYRELAERVKRPTAVRAVGATNGRNKLSIVVPCHRVIGSTGKLTGYAGGLWRKEWLLQHEKQVAHQPQ
>Mature_159_residues
GTYKLDVETPIGVLEIIGTEQVVQSILFVERESKRSMEQDVPNVLRQCRDELIAYFRGERMTFTFPTEQIGTTFQHDVWD
ALQTIPYAETASYRELAERVKRPTAVRAVGATNGRNKLSIVVPCHRVIGSTGKLTGYAGGLWRKEWLLQHEKQVAHQPQ

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=168, Percent_Identity=32.7380952380952, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=44.0993788819876, Blast_Score=120, Evalue=4e-29,
Organism=Escherichia coli, GI1788542, Length=103, Percent_Identity=45.6310679611651, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=51.25, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=51.25, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=84, Percent_Identity=44.047619047619, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137554, Length=145, Percent_Identity=35.1724137931034, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18216; Mature: 18085

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTYKLDVETPIGVLEIIGTEQVVQSILFVERESKRSMEQDVPNVLRQCRDELIAYFRGE
CCCEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RMTFTFPTEQIGTTFQHDVWDALQTIPYAETASYRELAERVKRPTAVRAVGATNGRNKLS
EEEEECCHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCEEE
IVVPCHRVIGSTGKLTGYAGGLWRKEWLLQHEKQVAHQPQ
EEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GTYKLDVETPIGVLEIIGTEQVVQSILFVERESKRSMEQDVPNVLRQCRDELIAYFRGE
CCEEEECCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RMTFTFPTEQIGTTFQHDVWDALQTIPYAETASYRELAERVKRPTAVRAVGATNGRNKLS
EEEEECCHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHEEEECCCCCCCEEE
IVVPCHRVIGSTGKLTGYAGGLWRKEWLLQHEKQVAHQPQ
EEEEHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA