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The map label for this gene is gyrA [H]

Identifier: 229917450

GI number: 229917450

Start: 1691744

End: 1694311

Strand: Reverse

Name: gyrA [H]

Synonym: EAT1b_1725

Alternate gene names: 229917450

Gene position: 1694311-1691744 (Counterclockwise)

Preceding gene: 229917451

Following gene: 229917449

Centisome position: 56.48

GC content: 49.69

Gene sequence:

>2568_bases
ATGGCAGAACAAGAAAATATCAAGGATATAAATATTAGCCAGGAGATGCGAACGTCGTTCATGGACTACGCGATGAGCGT
CATCGTATCACGTGCCCTTCCGGACGTGCGAGATGGATTGAAGCCGGTTCACCGTCGTATTTTATATGCGATGAACGAAC
TAGGTATTCGCGCGGACAAGCCACATAAGAAGTCGGCGCGTGTCGTCGGGGACGTCATCGGTAAGTACCACCCACACGGT
GACTCAGCCGTTTATGAGACGATGGTACGCATGGCTCAGGACTTCAGCTATCGCTACGAATTAGTCGACGGTCACGGGAA
CTTCGGTTCAGTCGATGGCGACTCGGCCGCTGCGATGCGTTATACCGAGGCACGTATGTCTAAAATTGCGATGGAACTCG
TACGAGACATCGGAAAAAACACGATTGATTATCAACCGAACTTCGATGGTGAAGAAAAAGAACCGGTCGTACTTCCGGCA
CGTTTCCCGAACTTCCTTGTGAACGGGACGAGCGGAATTGCCGTTGGGATGGCGACGAACGTTCCGCCTCATAACTTGAA
CGAGGTCATCGATGGTGTACTGGCGCTTTCTCACAACCCGGACATCACGATTGCAGAATTGATGCAACATATCCCGGGTC
CTGATTTCCCGACTGCGGGAGAGATTTTGGGACGTAGTGGGATTCGTCGTGCATATGAAACTGGACGCGGATCGATTACG
ATGCGTGCGAAAGCTGAAATCGAGGTCTTGAAAAATGGCCGTGAACGGATCCTCGTGCACGAGATTCCTTACCAGGTCAA
CAAAGCGCGTCTCGTTGAGAAGATTGCCGATTTGGTTCGCGACAAGAAAATCGAGGGGATCACGGACTTACGCGATGAAT
CAGACCGTAATGGGATGCGCATCGTGATTGAAGTTCGCCGCGACGCGAATGCGAGTGTCATCTTGAACAACTTGTACAAG
CAAACACCGATGCAAACGACGTTCGGCGTCAACATGCTCGCGCTTGTAGGCGGTCGTCCAAAAACGTTGAACATCAAACA
AGCAATCTATTACTACATCGAACACCAAAAAGAGGTCGTGCGTCGTCGGACGCAATTCGATTTAGACAAAGCGGAAGCAC
GTGCCCACATTTTAGAAGGTCTTCGTGTTGCCTTGGATCACTTGGATGAAGTCATCGCCATCATCCGTAGCACGCGTACA
GGTGACGAGGCTCGTGAAAAGTTGATTGCTCGCTTTGGACTTTCGACTGAACAGACGCAAGCCATCTTAGATATGCGTCT
CCAACGCTTGACTGGTTTGGAACGCGAGAAGATTGAAGGCGAATATCGTGAAGTGAAAGCGTTAATCGAAGAACTTCGTG
CGATTCTCGGAGACGAAGAATTGCTCCTCGATATCATTCGTAACGAGTTAATCGAAATCAAAGAGCGATATGGTGATGCT
CGTCGTACGGTGATTCGTACGGATATCGTTGAACTCGAGGACGAAGATTTGATTCCAGTTCGCGACATGATGATTACGCT
CACGAGCGAAGGATATATCAAGTCGATCCCATCGGATTCATATCGGACACAGCGCCGTGGTGGACGTGGGAAACAAGGTA
TGGGAACGAATGATGAAGACTTTGTGCTTCGTCTGCTCTCCGCGTCGACACACGACACCATCCTGTTCTTCACCAACTTC
GGACGCGTCTTCCGTCTCAAAGGATACGAAATTCCTGAGATGAGCCGTACCGCGAAAGGGATGCCAATCATCAACTTGCT
TCAAATCGACAAAGGTGAAAAAGTCGAAACGATGATTCCGGTCGATTTCAATAGTTACTTGGCTAACCAAACGGTAGCGG
AAGAGACACAGGATGAATCGGAAGAAGTGGCAGACGAGCAGTTGTCTCTCGTCTTCATCACGAAAGAAGGATTGATTAAG
CGTTCGCCACTTTCGGCATACGCCCGAATCAACAAAAACGGTCTACGTGCCATCAGCTTGAATGAGACAGACGAGCTCGT
CACGGTTCGCTTAGCGAAGAGCGACGATGAAATGTTGATCGTTACACGCCAAGGGATGTCGATTCGCTTCCCTCTCGAAG
GAGAAGTTCGTTCGATGGGACGCACGGCACGAGGCGTGCGCGCGATTCGATTGAAGAAGGAAGACGATTTGGTCGTCTCG
ATGGAAATCGTTCGCACCACGCAAGACGTCTTGATTATCACTGAAAAAGGATACGGAAAACGTACGGCCATGGAGCAGTT
CCGCACACAAGGTCGTGGTGGTTCCGGTATCATCGGAATTAAGACAGATCGTGGAACAGTCGTCGGGATGCGTGTCGTCG
ATGAAGACGATGACATCATGCTGATGACCGAACTCGGTGTTGCGATTCGAATCGATTCACAAACGATCTCTCAAATGGGT
CGAAGTACTCGCGGTGTGAAAGTGATGAACATCGAAGAGGGCGATCGTTTGGCGACCATTGCAAAGTTGAAAAAAGATGA
GTTAGAAGAGGAATCAGCAGTCGATTTGGAGAATGGGTTTGAGGATGAAGCACCTGAAACCGACGAATCGTCTGATGTGA
ATGAGTAA

Upstream 100 bases:

>100_bases
CTCATGGGTGACAACGTCGAACCACGCCGTGACTTTATTCAATCTCACGCCCATTACGTGAAGAACCTCGATATTTAATA
AGAAGGGTAGGCAATAACGA

Downstream 100 bases:

>100_bases
GACGAGAGGTCGAACGGTGTTTCCGTTCGACCTTTTCATATATTAGGAGGAATTCGCGTGCGTTTGAACTTATCTACTGT
ACATGTTGGGATGACGCTAT

Product: DNA gyrase, A subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 855; Mature: 854

Protein sequence:

>855_residues
MAEQENIKDINISQEMRTSFMDYAMSVIVSRALPDVRDGLKPVHRRILYAMNELGIRADKPHKKSARVVGDVIGKYHPHG
DSAVYETMVRMAQDFSYRYELVDGHGNFGSVDGDSAAAMRYTEARMSKIAMELVRDIGKNTIDYQPNFDGEEKEPVVLPA
RFPNFLVNGTSGIAVGMATNVPPHNLNEVIDGVLALSHNPDITIAELMQHIPGPDFPTAGEILGRSGIRRAYETGRGSIT
MRAKAEIEVLKNGRERILVHEIPYQVNKARLVEKIADLVRDKKIEGITDLRDESDRNGMRIVIEVRRDANASVILNNLYK
QTPMQTTFGVNMLALVGGRPKTLNIKQAIYYYIEHQKEVVRRRTQFDLDKAEARAHILEGLRVALDHLDEVIAIIRSTRT
GDEAREKLIARFGLSTEQTQAILDMRLQRLTGLEREKIEGEYREVKALIEELRAILGDEELLLDIIRNELIEIKERYGDA
RRTVIRTDIVELEDEDLIPVRDMMITLTSEGYIKSIPSDSYRTQRRGGRGKQGMGTNDEDFVLRLLSASTHDTILFFTNF
GRVFRLKGYEIPEMSRTAKGMPIINLLQIDKGEKVETMIPVDFNSYLANQTVAEETQDESEEVADEQLSLVFITKEGLIK
RSPLSAYARINKNGLRAISLNETDELVTVRLAKSDDEMLIVTRQGMSIRFPLEGEVRSMGRTARGVRAIRLKKEDDLVVS
MEIVRTTQDVLIITEKGYGKRTAMEQFRTQGRGGSGIIGIKTDRGTVVGMRVVDEDDDIMLMTELGVAIRIDSQTISQMG
RSTRGVKVMNIEEGDRLATIAKLKKDELEEESAVDLENGFEDEAPETDESSDVNE

Sequences:

>Translated_855_residues
MAEQENIKDINISQEMRTSFMDYAMSVIVSRALPDVRDGLKPVHRRILYAMNELGIRADKPHKKSARVVGDVIGKYHPHG
DSAVYETMVRMAQDFSYRYELVDGHGNFGSVDGDSAAAMRYTEARMSKIAMELVRDIGKNTIDYQPNFDGEEKEPVVLPA
RFPNFLVNGTSGIAVGMATNVPPHNLNEVIDGVLALSHNPDITIAELMQHIPGPDFPTAGEILGRSGIRRAYETGRGSIT
MRAKAEIEVLKNGRERILVHEIPYQVNKARLVEKIADLVRDKKIEGITDLRDESDRNGMRIVIEVRRDANASVILNNLYK
QTPMQTTFGVNMLALVGGRPKTLNIKQAIYYYIEHQKEVVRRRTQFDLDKAEARAHILEGLRVALDHLDEVIAIIRSTRT
GDEAREKLIARFGLSTEQTQAILDMRLQRLTGLEREKIEGEYREVKALIEELRAILGDEELLLDIIRNELIEIKERYGDA
RRTVIRTDIVELEDEDLIPVRDMMITLTSEGYIKSIPSDSYRTQRRGGRGKQGMGTNDEDFVLRLLSASTHDTILFFTNF
GRVFRLKGYEIPEMSRTAKGMPIINLLQIDKGEKVETMIPVDFNSYLANQTVAEETQDESEEVADEQLSLVFITKEGLIK
RSPLSAYARINKNGLRAISLNETDELVTVRLAKSDDEMLIVTRQGMSIRFPLEGEVRSMGRTARGVRAIRLKKEDDLVVS
MEIVRTTQDVLIITEKGYGKRTAMEQFRTQGRGGSGIIGIKTDRGTVVGMRVVDEDDDIMLMTELGVAIRIDSQTISQMG
RSTRGVKVMNIEEGDRLATIAKLKKDELEEESAVDLENGFEDEAPETDESSDVNE
>Mature_854_residues
AEQENIKDINISQEMRTSFMDYAMSVIVSRALPDVRDGLKPVHRRILYAMNELGIRADKPHKKSARVVGDVIGKYHPHGD
SAVYETMVRMAQDFSYRYELVDGHGNFGSVDGDSAAAMRYTEARMSKIAMELVRDIGKNTIDYQPNFDGEEKEPVVLPAR
FPNFLVNGTSGIAVGMATNVPPHNLNEVIDGVLALSHNPDITIAELMQHIPGPDFPTAGEILGRSGIRRAYETGRGSITM
RAKAEIEVLKNGRERILVHEIPYQVNKARLVEKIADLVRDKKIEGITDLRDESDRNGMRIVIEVRRDANASVILNNLYKQ
TPMQTTFGVNMLALVGGRPKTLNIKQAIYYYIEHQKEVVRRRTQFDLDKAEARAHILEGLRVALDHLDEVIAIIRSTRTG
DEAREKLIARFGLSTEQTQAILDMRLQRLTGLEREKIEGEYREVKALIEELRAILGDEELLLDIIRNELIEIKERYGDAR
RTVIRTDIVELEDEDLIPVRDMMITLTSEGYIKSIPSDSYRTQRRGGRGKQGMGTNDEDFVLRLLSASTHDTILFFTNFG
RVFRLKGYEIPEMSRTAKGMPIINLLQIDKGEKVETMIPVDFNSYLANQTVAEETQDESEEVADEQLSLVFITKEGLIKR
SPLSAYARINKNGLRAISLNETDELVTVRLAKSDDEMLIVTRQGMSIRFPLEGEVRSMGRTARGVRAIRLKKEDDLVVSM
EIVRTTQDVLIITEKGYGKRTAMEQFRTQGRGGSGIIGIKTDRGTVVGMRVVDEDDDIMLMTELGVAIRIDSQTISQMGR
STRGVKVMNIEEGDRLATIAKLKKDELEEESAVDLENGFEDEAPETDESSDVNE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=320, Percent_Identity=25.3125, Blast_Score=82, Evalue=3e-15,
Organism=Homo sapiens, GI19913406, Length=321, Percent_Identity=24.2990654205607, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788562, Length=883, Percent_Identity=49.4903737259343, Blast_Score=811, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=684, Percent_Identity=38.3040935672515, Blast_Score=444, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=192, Percent_Identity=31.25, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI212645657, Length=202, Percent_Identity=28.7128712871287, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI212645845, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=204, Percent_Identity=27.4509803921569, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136538, Length=243, Percent_Identity=27.5720164609054, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR010235
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV; PF08780 NTase_sub_bind [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 96337; Mature: 96205

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEQENIKDINISQEMRTSFMDYAMSVIVSRALPDVRDGLKPVHRRILYAMNELGIRADK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
PHKKSARVVGDVIGKYHPHGDSAVYETMVRMAQDFSYRYELVDGHGNFGSVDGDSAAAMR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
YTEARMSKIAMELVRDIGKNTIDYQPNFDGEEKEPVVLPARFPNFLVNGTSGIAVGMATN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECC
VPPHNLNEVIDGVLALSHNPDITIAELMQHIPGPDFPTAGEILGRSGIRRAYETGRGSIT
CCCCCHHHHHHHHHEECCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
MRAKAEIEVLKNGRERILVHEIPYQVNKARLVEKIADLVRDKKIEGITDLRDESDRNGMR
EEEHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCE
IVIEVRRDANASVILNNLYKQTPMQTTFGVNMLALVGGRPKTLNIKQAIYYYIEHQKEVV
EEEEEECCCCHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
RRRTQFDLDKAEARAHILEGLRVALDHLDEVIAIIRSTRTGDEAREKLIARFGLSTEQTQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
AILDMRLQRLTGLEREKIEGEYREVKALIEELRAILGDEELLLDIIRNELIEIKERYGDA
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHH
RRTVIRTDIVELEDEDLIPVRDMMITLTSEGYIKSIPSDSYRTQRRGGRGKQGMGTNDED
HHHHHHHHHEECCCCCCCCHHHEEEEEECCCCEECCCCCHHHHHHCCCCCCCCCCCCCHH
FVLRLLSASTHDTILFFTNFGRVFRLKGYEIPEMSRTAKGMPIINLLQIDKGEKVETMIP
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCHHHHCCCCCEEEEEECCCCCCEEEEEC
VDFNSYLANQTVAEETQDESEEVADEQLSLVFITKEGLIKRSPLSAYARINKNGLRAISL
CCHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEC
NETDELVTVRLAKSDDEMLIVTRQGMSIRFPLEGEVRSMGRTARGVRAIRLKKEDDLVVS
CCCCCEEEEEEECCCCCEEEEEECCCEEEECCCHHHHHHCHHHCCEEEEEEECCCCEEEE
MEIVRTTQDVLIITEKGYGKRTAMEQFRTQGRGGSGIIGIKTDRGTVVGMRVVDEDDDIM
EHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCEE
LMTELGVAIRIDSQTISQMGRSTRGVKVMNIEEGDRLATIAKLKKDELEEESAVDLENGF
EEEECCEEEEECHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCCHHCCC
EDEAPETDESSDVNE
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
AEQENIKDINISQEMRTSFMDYAMSVIVSRALPDVRDGLKPVHRRILYAMNELGIRADK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
PHKKSARVVGDVIGKYHPHGDSAVYETMVRMAQDFSYRYELVDGHGNFGSVDGDSAAAMR
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHH
YTEARMSKIAMELVRDIGKNTIDYQPNFDGEEKEPVVLPARFPNFLVNGTSGIAVGMATN
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCEEEECCCCEEEEEECC
VPPHNLNEVIDGVLALSHNPDITIAELMQHIPGPDFPTAGEILGRSGIRRAYETGRGSIT
CCCCCHHHHHHHHHEECCCCCEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
MRAKAEIEVLKNGRERILVHEIPYQVNKARLVEKIADLVRDKKIEGITDLRDESDRNGMR
EEEHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCCCCE
IVIEVRRDANASVILNNLYKQTPMQTTFGVNMLALVGGRPKTLNIKQAIYYYIEHQKEVV
EEEEEECCCCHHHHHHHHHHHCCCCHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHH
RRRTQFDLDKAEARAHILEGLRVALDHLDEVIAIIRSTRTGDEAREKLIARFGLSTEQTQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHH
AILDMRLQRLTGLEREKIEGEYREVKALIEELRAILGDEELLLDIIRNELIEIKERYGDA
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCHH
RRTVIRTDIVELEDEDLIPVRDMMITLTSEGYIKSIPSDSYRTQRRGGRGKQGMGTNDED
HHHHHHHHHEECCCCCCCCHHHEEEEEECCCCEECCCCCHHHHHHCCCCCCCCCCCCCHH
FVLRLLSASTHDTILFFTNFGRVFRLKGYEIPEMSRTAKGMPIINLLQIDKGEKVETMIP
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCHHHHCCCCCEEEEEECCCCCCEEEEEC
VDFNSYLANQTVAEETQDESEEVADEQLSLVFITKEGLIKRSPLSAYARINKNGLRAISL
CCHHHHHHCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHCCCCCCEEEEC
NETDELVTVRLAKSDDEMLIVTRQGMSIRFPLEGEVRSMGRTARGVRAIRLKKEDDLVVS
CCCCCEEEEEEECCCCCEEEEEECCCEEEECCCHHHHHHCHHHCCEEEEEEECCCCEEEE
MEIVRTTQDVLIITEKGYGKRTAMEQFRTQGRGGSGIIGIKTDRGTVVGMRVVDEDDDIM
EHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCCCEE
LMTELGVAIRIDSQTISQMGRSTRGVKVMNIEEGDRLATIAKLKKDELEEESAVDLENGF
EEEECCEEEEECHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCHHHCCCHHCCC
EDEAPETDESSDVNE
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10427704; 11058132 [H]