Click here to switch to the map view.

The map label for this gene is tilS [H]

Identifier: 229917403

GI number: 229917403

Start: 1626019

End: 1627245

Strand: Reverse

Name: tilS [H]

Synonym: EAT1b_1678

Alternate gene names: 229917403

Gene position: 1627245-1626019 (Counterclockwise)

Preceding gene: 229917404

Following gene: 229917402

Centisome position: 54.24

GC content: 49.23

Gene sequence:

>1227_bases
ATGAAAAAGGTACTCGTCGCTGTGTCCGGTGGAGTCGATTCCATCGTCTTATTGGACCGTCTCGTCAAGCAAGGATGGGA
TGTGGGGGTAGCGCACGTCCATCATGGCTTACGAGAAGCATCAGATGAAGAGTACACGTTCGTCGCGCACGTAGCGGCAT
CCTATCAAGTCGCGTTTCATGGGACGCGTCTCCATTTTCAAGAAGGAGCGTCACAGGCTCATTACCGTAAGGCCCGTTAT
CACTTTTTTGAAGACGTCATGAATGAACATGGATATGATACACTGGCCACGGCTCATCATGCGGATGATGTATTGGAGAC
GGTGCTCATTCAACTTCACCGAAACGTGATCGAAGTGAAAGGAATTCCCGAGAGAAGAGTGTTCGGGCGTGGGACGTTGA
TCCGTCCTTTATTAAAAGAAACAAAGGCTCAACTCATCGAGTACGCTAGGAAGAACGAACTTGCCTGGAGGGAAGATGCC
ACCAATCAGGAAGTGACCTATTTACGGAACCGGATCCGTCATGAAATCATTCCGAGGCTGAAACAAGTGTGGCCAACAGT
CGTTGACGATGTATACGATACGGCGAAACAGGTATACGCTGAATGGGACAGGCGTTACCAGTCATGTGAGCAATGGATTG
AAGCAAACGTAGAGCTTGGAATGAAAGCGTTTCATGTGAAACTTGATGAATTCAATCGCTTATCCTCGCTCGAACGGTAT
ACGGTCGCTCGCTTGCTATCCCGTCGCTATGGGGTAGAGGTCAATGAGGCGCTTGAGAAGCTAAGGACGAGTCAAAAGGA
TACCGGTACATACCACCTGTCGGGTGAGTGGTTCATGATGAAGCAGAATAGGGTACTTAATCTTGTACAACGTTCAGCAG
AAGCGTCACTTCATCCCATTCAGGTCGAGTCGTTGCCGGCTGTCGTTCAATTTGGAAAGCGCACGGTTTCGCTGTCCATC
GAAGAAGGGCGAGAAGGTATTCCACTAAATGAAATCACTTGCCCGTTGACGATTCGTTCGCTCGCTCCGGGGGACCGGAT
GAAGTTGAACGTGGGGACAAAAAAAGTGTCCCGCATCCTAATCGATGAAAAGGTTGAACGAACCATTCGACCGACTCTCC
CGTTGCTTGAGGACGCCTCAGGGCGAATTCTCGCGATTGTCGGGGTAAGAGTTTCAGATTTTCTAGACAAGTCGGATGCG
ATTTGTCTACGATTAATGGTAAAATGA

Upstream 100 bases:

>100_bases
CGGTAACACGGGTTCGAATCCCGTACGGGTCACTTAACCTAAGCGTAAAGAAGCCATCTCAATCGAGATGGCTTCTTTTG
TTATATCAAGGAGGGAATTC

Downstream 100 bases:

>100_bases
TGATTGTGTCGAAACAGAAAGAGGAGGATTTCAAGATGTCATTAATGAACGATATGGAGAAAATTTTAATCTCACGTGAA
GAGATTCAAGAAAAGGTGAA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKKVLVAVSGGVDSIVLLDRLVKQGWDVGVAHVHHGLREASDEEYTFVAHVAASYQVAFHGTRLHFQEGASQAHYRKARY
HFFEDVMNEHGYDTLATAHHADDVLETVLIQLHRNVIEVKGIPERRVFGRGTLIRPLLKETKAQLIEYARKNELAWREDA
TNQEVTYLRNRIRHEIIPRLKQVWPTVVDDVYDTAKQVYAEWDRRYQSCEQWIEANVELGMKAFHVKLDEFNRLSSLERY
TVARLLSRRYGVEVNEALEKLRTSQKDTGTYHLSGEWFMMKQNRVLNLVQRSAEASLHPIQVESLPAVVQFGKRTVSLSI
EEGREGIPLNEITCPLTIRSLAPGDRMKLNVGTKKVSRILIDEKVERTIRPTLPLLEDASGRILAIVGVRVSDFLDKSDA
ICLRLMVK

Sequences:

>Translated_408_residues
MKKVLVAVSGGVDSIVLLDRLVKQGWDVGVAHVHHGLREASDEEYTFVAHVAASYQVAFHGTRLHFQEGASQAHYRKARY
HFFEDVMNEHGYDTLATAHHADDVLETVLIQLHRNVIEVKGIPERRVFGRGTLIRPLLKETKAQLIEYARKNELAWREDA
TNQEVTYLRNRIRHEIIPRLKQVWPTVVDDVYDTAKQVYAEWDRRYQSCEQWIEANVELGMKAFHVKLDEFNRLSSLERY
TVARLLSRRYGVEVNEALEKLRTSQKDTGTYHLSGEWFMMKQNRVLNLVQRSAEASLHPIQVESLPAVVQFGKRTVSLSI
EEGREGIPLNEITCPLTIRSLAPGDRMKLNVGTKKVSRILIDEKVERTIRPTLPLLEDASGRILAIVGVRVSDFLDKSDA
ICLRLMVK
>Mature_408_residues
MKKVLVAVSGGVDSIVLLDRLVKQGWDVGVAHVHHGLREASDEEYTFVAHVAASYQVAFHGTRLHFQEGASQAHYRKARY
HFFEDVMNEHGYDTLATAHHADDVLETVLIQLHRNVIEVKGIPERRVFGRGTLIRPLLKETKAQLIEYARKNELAWREDA
TNQEVTYLRNRIRHEIIPRLKQVWPTVVDDVYDTAKQVYAEWDRRYQSCEQWIEANVELGMKAFHVKLDEFNRLSSLERY
TVARLLSRRYGVEVNEALEKLRTSQKDTGTYHLSGEWFMMKQNRVLNLVQRSAEASLHPIQVESLPAVVQFGKRTVSLSI
EEGREGIPLNEITCPLTIRSLAPGDRMKLNVGTKKVSRILIDEKVERTIRPTLPLLEDASGRILAIVGVRVSDFLDKSDA
ICLRLMVK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=205, Percent_Identity=37.5609756097561, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 46780; Mature: 46780

Theoretical pI: Translated: 8.71; Mature: 8.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVLVAVSGGVDSIVLLDRLVKQGWDVGVAHVHHGLREASDEEYTFVAHVAASYQVAFH
CCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHEEEEEE
GTRLHFQEGASQAHYRKARYHFFEDVMNEHGYDTLATAHHADDVLETVLIQLHRNVIEVK
CCEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
GIPERRVFGRGTLIRPLLKETKAQLIEYARKNELAWREDATNQEVTYLRNRIRHEIIPRL
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KQVWPTVVDDVYDTAKQVYAEWDRRYQSCEQWIEANVELGMKAFHVKLDEFNRLSSLERY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHEEEEHHHHHHHHHHHHH
TVARLLSRRYGVEVNEALEKLRTSQKDTGTYHLSGEWFMMKQNRVLNLVQRSAEASLHPI
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHCCCCCE
QVESLPAVVQFGKRTVSLSIEEGREGIPLNEITCPLTIRSLAPGDRMKLNVGTKKVSRIL
EHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHH
IDEKVERTIRPTLPLLEDASGRILAIVGVRVSDFLDKSDAICLRLMVK
HHHHHHHHHCCCCCHHCCCCCCEEEEECCHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure
MKKVLVAVSGGVDSIVLLDRLVKQGWDVGVAHVHHGLREASDEEYTFVAHVAASYQVAFH
CCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEHHHHEEEEEE
GTRLHFQEGASQAHYRKARYHFFEDVMNEHGYDTLATAHHADDVLETVLIQLHRNVIEVK
CCEEEHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEC
GIPERRVFGRGTLIRPLLKETKAQLIEYARKNELAWREDATNQEVTYLRNRIRHEIIPRL
CCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KQVWPTVVDDVYDTAKQVYAEWDRRYQSCEQWIEANVELGMKAFHVKLDEFNRLSSLERY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHEEEEHHHHHHHHHHHHH
TVARLLSRRYGVEVNEALEKLRTSQKDTGTYHLSGEWFMMKQNRVLNLVQRSAEASLHPI
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHCCCCCE
QVESLPAVVQFGKRTVSLSIEEGREGIPLNEITCPLTIRSLAPGDRMKLNVGTKKVSRIL
EHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCCCEEEECCHHHHHHHH
IDEKVERTIRPTLPLLEDASGRILAIVGVRVSDFLDKSDAICLRLMVK
HHHHHHHHHCCCCCHHCCCCCCEEEEECCHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA