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The map label for this gene is tenA [H]

Identifier: 229917255

GI number: 229917255

Start: 1496347

End: 1497021

Strand: Reverse

Name: tenA [H]

Synonym: EAT1b_1530

Alternate gene names: 229917255

Gene position: 1497021-1496347 (Counterclockwise)

Preceding gene: 229917257

Following gene: 229917254

Centisome position: 49.9

GC content: 47.85

Gene sequence:

>675_bases
ATGAAATTTACAGATCACTTACGTCAAGAAGCGGATGGATTGTTTGAGGCAAGCTTTCATCACCCATTTGTTCGTTCACT
CGGAGAAGGGACGCTCGATGAGAAGAAGTTCAGACATTACGTCATGCAAGATTCGTATTACTTGAATCAATTCTCAAAAG
TGCAGGCCAAAGGGGCAACCCTCGCTCCGACGATGGAACTATCGAGTCGCTTTTTGACGCATGCCTTACACACGATTGAG
GCGGAACTCTCCCTTCATCGAGAGTTCTTTACGATGCTCAACGTGACGGATGTCGAATTGGCGACATTTGAGCCAGCACC
GACCGCTTATGCGTATGCGCTCCATATGCAACAGGCAGGACCGACACTCGGTGACGTATTGGCGTCAATCCTTCCGTGTT
ATTGGGTATATTACGAAATCGGTGAACGCTTAAAAGATAAACGACCCGACCATCCGATTTATTCAGCTTGGATTGCGACA
TACGGTTCGGAGTGGTTCCGTGAGCTGGTGGAAGAACAGATTGATCGGTTGGATGAATTGGCTGGACAAGCGACAGAAGA
AGAACGGCTTCGTTACACAAAACTCTTCTTGAAGAGTTGCTATTACGAAGTCGCTTTTTGGGAAATGGCGTGGACGCTTG
AGTCGTGGACATTGTCGCAGGAGGTCAACCAATGA

Upstream 100 bases:

>100_bases
CGAGACGACTCATATGGCTTCACCTATATATGACTGTTCGAGACGCCGCTTCCGTTATGGGAGGCGGCGTCTTTTTATTA
GTTTATAAGGGGGAGTTAGT

Downstream 100 bases:

>100_bases
GACATGAGATTTGGAACGAGATTCGACAGCAGGCACCGTTGATTCACAACATCACGAACACGGTTGTGGCGAATTTTTCA
GCGAATGGACTGCTCGCAAT

Product: transcriptional activator, TenA family

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MKFTDHLRQEADGLFEASFHHPFVRSLGEGTLDEKKFRHYVMQDSYYLNQFSKVQAKGATLAPTMELSSRFLTHALHTIE
AELSLHREFFTMLNVTDVELATFEPAPTAYAYALHMQQAGPTLGDVLASILPCYWVYYEIGERLKDKRPDHPIYSAWIAT
YGSEWFRELVEEQIDRLDELAGQATEEERLRYTKLFLKSCYYEVAFWEMAWTLESWTLSQEVNQ

Sequences:

>Translated_224_residues
MKFTDHLRQEADGLFEASFHHPFVRSLGEGTLDEKKFRHYVMQDSYYLNQFSKVQAKGATLAPTMELSSRFLTHALHTIE
AELSLHREFFTMLNVTDVELATFEPAPTAYAYALHMQQAGPTLGDVLASILPCYWVYYEIGERLKDKRPDHPIYSAWIAT
YGSEWFRELVEEQIDRLDELAGQATEEERLRYTKLFLKSCYYEVAFWEMAWTLESWTLSQEVNQ
>Mature_224_residues
MKFTDHLRQEADGLFEASFHHPFVRSLGEGTLDEKKFRHYVMQDSYYLNQFSKVQAKGATLAPTMELSSRFLTHALHTIE
AELSLHREFFTMLNVTDVELATFEPAPTAYAYALHMQQAGPTLGDVLASILPCYWVYYEIGERLKDKRPDHPIYSAWIAT
YGSEWFRELVEEQIDRLDELAGQATEEERLRYTKLFLKSCYYEVAFWEMAWTLESWTLSQEVNQ

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=202, Percent_Identity=25.7425742574257, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325378, Length=206, Percent_Identity=25.7281553398058, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26217; Mature: 26217

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTDHLRQEADGLFEASFHHPFVRSLGEGTLDEKKFRHYVMQDSYYLNQFSKVQAKGAT
CCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC
LAPTMELSSRFLTHALHTIEAELSLHREFFTMLNVTDVELATFEPAPTAYAYALHMQQAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHC
PTLGDVLASILPCYWVYYEIGERLKDKRPDHPIYSAWIATYGSEWFRELVEEQIDRLDEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGQATEEERLRYTKLFLKSCYYEVAFWEMAWTLESWTLSQEVNQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFTDHLRQEADGLFEASFHHPFVRSLGEGTLDEKKFRHYVMQDSYYLNQFSKVQAKGAT
CCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCC
LAPTMELSSRFLTHALHTIEAELSLHREFFTMLNVTDVELATFEPAPTAYAYALHMQQAG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHC
PTLGDVLASILPCYWVYYEIGERLKDKRPDHPIYSAWIATYGSEWFRELVEEQIDRLDEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGQATEEERLRYTKLFLKSCYYEVAFWEMAWTLESWTLSQEVNQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]