Click here to switch to the map view.

The map label for this gene is hmp [H]

Identifier: 229917142

GI number: 229917142

Start: 1386291

End: 1387391

Strand: Reverse

Name: hmp [H]

Synonym: EAT1b_1416

Alternate gene names: 229917142

Gene position: 1387391-1386291 (Counterclockwise)

Preceding gene: 229917144

Following gene: 229917136

Centisome position: 46.25

GC content: 49.77

Gene sequence:

>1101_bases
ATGTTAGATCAACAAACGATTGAGATTGTAAAGGCGACGGCACCAGTTTTGAAAGAAAATGGTGTGACGATCACGAAGCA
TTTTTATCAACGAATGTTTGAACAAAATCCAGAAGTTCGTCACTTCTTCAACCATACGAACCAAAAGCGAGGACGTCAGC
CGGAAGCACTCGCGAACGCGGTCTATGCGGCTGCGCTTCATATCGACCGACTCGAAGCGATTCTTCCTGCCATCCAACCA
GCACTTCATAAACATAAAAGTTTGAACATCCGCCCGGAGCATTATCCGATCGTTGGTGAGAACTTGCTTGCGGCGATTCA
AGACGTACTCGGTGAAGCTGCAACACCTGACATCATCGATGCATGGGCAAAAGCTTACGGTGTGATTGCCGATGTCTTCA
TCTCACTCGAGAAAAAAATGTATGATGACGCGCCATGGGTCGGATTCAAACCGTTCGTCATCGAAGAAATCATAGTAGAT
ACACCTGAAGTGAAACGATTCCGTCTCGTCGCGAAAGATGGAGTTATTGGCACAGCCATCCCAGGACAATATGTCTCGGT
TCAAGCGCGCATTGCAGGAGAGGACATCCTACATCACCGTCAATATAGTGTCATCGAGACGACAGAAGACGGCTACTGGA
TTGCGCCGAAAGCGGAGGGACTCGTCTCAAACTGGCTTCATGAGCAGACGGTCGGAACAGAAGTCCCGATGAGCGCACCA
GCAGGAGAATTCATCCTTGAAACGTCTGAACGTCCGCTCACACTCATCGCGGGAGGAATCGGGATTACGCCCCTCTTCAA
CATGGCGAAGACGGCACTCGAACAAGGACGTCCTGTCACGTTATTGCACGCGGTTCGGAGTATGGACCTTCGTCCACTCG
GAGACGAACTCGATGAACTCGTGAAGGAAGGGCTTCATCTGCTCACACATGCAGATGACGTATCGGGTTGCATGAATAAG
TCACACCTTGAGGAACTTGATGTGGAAGGGCATGATGTTTATACGTGCGGACCGAATGCGATGATGGAAACGGTCGTTCG
CGCTATTCCACAGGCACGATATGAATTTTTCGGGCCGTCTGGAGTTCTTGCGACTAACTAA

Upstream 100 bases:

>100_bases
CAGAAACGTGAAATCTACTTTAAACAAGTATTTAATATATTGCTTTAAAGTGACTTGACTCACATAATGAACGTGTACAA
AACAAAAGGGGGAAATTCAC

Downstream 100 bases:

>100_bases
AAAAGACGACTGGAAAAAGTCGTCTTGATGCACAATATAGGTTATTCCGTGAGTTTACTTGAATGTATGACTTGTTTGTT
GGCATCCAATGTTTCAAGTG

Product: oxidoreductase FAD/NAD(P)-binding domain protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MLDQQTIEIVKATAPVLKENGVTITKHFYQRMFEQNPEVRHFFNHTNQKRGRQPEALANAVYAAALHIDRLEAILPAIQP
ALHKHKSLNIRPEHYPIVGENLLAAIQDVLGEAATPDIIDAWAKAYGVIADVFISLEKKMYDDAPWVGFKPFVIEEIIVD
TPEVKRFRLVAKDGVIGTAIPGQYVSVQARIAGEDILHHRQYSVIETTEDGYWIAPKAEGLVSNWLHEQTVGTEVPMSAP
AGEFILETSERPLTLIAGGIGITPLFNMAKTALEQGRPVTLLHAVRSMDLRPLGDELDELVKEGLHLLTHADDVSGCMNK
SHLEELDVEGHDVYTCGPNAMMETVVRAIPQARYEFFGPSGVLATN

Sequences:

>Translated_366_residues
MLDQQTIEIVKATAPVLKENGVTITKHFYQRMFEQNPEVRHFFNHTNQKRGRQPEALANAVYAAALHIDRLEAILPAIQP
ALHKHKSLNIRPEHYPIVGENLLAAIQDVLGEAATPDIIDAWAKAYGVIADVFISLEKKMYDDAPWVGFKPFVIEEIIVD
TPEVKRFRLVAKDGVIGTAIPGQYVSVQARIAGEDILHHRQYSVIETTEDGYWIAPKAEGLVSNWLHEQTVGTEVPMSAP
AGEFILETSERPLTLIAGGIGITPLFNMAKTALEQGRPVTLLHAVRSMDLRPLGDELDELVKEGLHLLTHADDVSGCMNK
SHLEELDVEGHDVYTCGPNAMMETVVRAIPQARYEFFGPSGVLATN
>Mature_366_residues
MLDQQTIEIVKATAPVLKENGVTITKHFYQRMFEQNPEVRHFFNHTNQKRGRQPEALANAVYAAALHIDRLEAILPAIQP
ALHKHKSLNIRPEHYPIVGENLLAAIQDVLGEAATPDIIDAWAKAYGVIADVFISLEKKMYDDAPWVGFKPFVIEEIIVD
TPEVKRFRLVAKDGVIGTAIPGQYVSVQARIAGEDILHHRQYSVIETTEDGYWIAPKAEGLVSNWLHEQTVGTEVPMSAP
AGEFILETSERPLTLIAGGIGITPLFNMAKTALEQGRPVTLLHAVRSMDLRPLGDELDELVKEGLHLLTHADDVSGCMNK
SHLEELDVEGHDVYTCGPNAMMETVVRAIPQARYEFFGPSGVLATN

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1017

COG function: function code C; Hemoglobin-like flavoprotein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=401, Percent_Identity=37.6558603491272, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788104, Length=194, Percent_Identity=34.020618556701, Blast_Score=62, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=397, Percent_Identity=37.7833753148615, Blast_Score=231, Evalue=2e-61,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR001834
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 40545; Mature: 40545

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDQQTIEIVKATAPVLKENGVTITKHFYQRMFEQNPEVRHFFNHTNQKRGRQPEALANA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCHHHHHHH
VYAAALHIDRLEAILPAIQPALHKHKSLNIRPEHYPIVGENLLAAIQDVLGEAATPDIID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
AWAKAYGVIADVFISLEKKMYDDAPWVGFKPFVIEEIIVDTPEVKRFRLVAKDGVIGTAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHEEEEECCCEEECCC
PGQYVSVQARIAGEDILHHRQYSVIETTEDGYWIAPKAEGLVSNWLHEQTVGTEVPMSAP
CCCEEEEEEEECHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCC
AGEFILETSERPLTLIAGGIGITPLFNMAKTALEQGRPVTLLHAVRSMDLRPLGDELDEL
CCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
VKEGLHLLTHADDVSGCMNKSHLEELDVEGHDVYTCGPNAMMETVVRAIPQARYEFFGPS
HHHHHHHHHCCHHHHHHHCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC
GVLATN
CCCCCC
>Mature Secondary Structure
MLDQQTIEIVKATAPVLKENGVTITKHFYQRMFEQNPEVRHFFNHTNQKRGRQPEALANA
CCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHCCHHHCCCCHHHHHHH
VYAAALHIDRLEAILPAIQPALHKHKSLNIRPEHYPIVGENLLAAIQDVLGEAATPDIID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHH
AWAKAYGVIADVFISLEKKMYDDAPWVGFKPFVIEEIIVDTPEVKRFRLVAKDGVIGTAI
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHEEEEECCCEEECCC
PGQYVSVQARIAGEDILHHRQYSVIETTEDGYWIAPKAEGLVSNWLHEQTVGTEVPMSAP
CCCEEEEEEEECHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCC
AGEFILETSERPLTLIAGGIGITPLFNMAKTALEQGRPVTLLHAVRSMDLRPLGDELDEL
CCCCEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHH
VKEGLHLLTHADDVSGCMNKSHLEELDVEGHDVYTCGPNAMMETVVRAIPQARYEFFGPS
HHHHHHHHHCCHHHHHHHCHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCC
GVLATN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10484179; 11058132 [H]