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The map label for this gene is ygiC [H]

Identifier: 229917139

GI number: 229917139

Start: 1383983

End: 1385212

Strand: Direct

Name: ygiC [H]

Synonym: EAT1b_1413

Alternate gene names: 229917139

Gene position: 1383983-1385212 (Clockwise)

Preceding gene: 229917138

Following gene: 229917140

Centisome position: 46.13

GC content: 49.35

Gene sequence:

>1230_bases
ATGACGCGTGACGAACTTTACAGTCAAATCCCTTATTTTTGGCCCGATTTAAACGATGAAGAATATGCCTTATATGATTG
TTTTGAGATGACCGCCTCGCAGGTTGCCTCTATCCGAGAGGCAACCGAGGCGGTTGACTCGATTTTTCGGAAGACGAATC
AACTATTACGCACCCTACCTGACGAAACGCTCCGACTGCTCGGTTACCCTGAGGCGAGTCTGCCGTTCTTACGGGAGAAG
ACGATACCGCAGGAGACGATCATCGGTCGTTACGACTGGATCATGAAAGATGATCAACTCAAGTTGATGGAGTTCAATGC
AGATACGCCGACCTTCATTAAGGAGCTATTTGATGTGAACGGCGTGGTCGCTTCCGAGTTCGGTCTACTCGACCCGAACA
GGGAAGCCACCCCCCAACTGAAAATTGAGCTGCGGAAAGCGATCGTCGCCGCGTGGAATCGACTCAAACGAGAAGGCACA
CCGAAAATCGTTTTTACGGCACATGACGATAACATCGAAGACAAGTGGACGGCACGATTTTTACAAGAAACGCTCGACGT
ACCGTCTGAATTCGTACCGTTGCACACTTTACTCGTCGACGAAGACGGCGTCTACACACCATCGGGTGAGCGAGTTGACA
TCTTATATCGCCAAACATATCCGATTGAGCACCTTGTGGACGACCGGGCTCCAGACGGAGACTTGATTGGCATTCGGTTA
CTTGAACTCGATCAACAAAAATACGTGTCACTGCTCAATCCCCTCTCTGCGTTTCTCATGCAATCGAAAGGCGTACAGGC
GCTGATTTGGGAATTGTATGAGACGGAGACACTATTCGATCAAGCCGAGCGCGACATCATCGGAACATATTTTTTACCGA
CTTACTTAGAAGACACACCGTTTTTCGGTGAATCGGACTATGTCAAAAAGCCGGCATTCGGACGTGAAGGGGACTCCGTC
ATCTTGTATACGAAAGATGGCAAACCGTTTCATCGCGAAACATTACAAACGTATGCGGAAGAAACGGCCGTGTACCAACA
GTTCATCGAACTCCCGACACGGATGACGACAACCGTCAAAGGTCGTATGGAGACACATTACATGATCGGATGTTTTTGCT
TGAACGGACACCCATCCGCACTCGGCGTCCGCGCCGGCAGCATGATCACGAACAACCAATCTTATTACTTAGCCGTTGGC
ACACCTATACAAAAGGAGACGAACTCATGA

Upstream 100 bases:

>100_bases
ACAAAGCCAACGGTGGTGCGACCGGTGCCGTATCGAACGGCTCAAATTCAGGATCGTCAAAATCTGGACTCGGCAGTGGC
TCTAAAGGTGGATTCGGCGG

Downstream 100 bases:

>100_bases
CATTCATCACACTCGATTCAATCTTAAGCTTCCTGGCCCACCTCGGGACAGGATTCGGTCTCATTCTCGCGGGACTCATC
ATCTTCGCACTAACGACCAA

Product: glutathionylspermidine synthase

Products: N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine; ADP; phosphate

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTRDELYSQIPYFWPDLNDEEYALYDCFEMTASQVASIREATEAVDSIFRKTNQLLRTLPDETLRLLGYPEASLPFLREK
TIPQETIIGRYDWIMKDDQLKLMEFNADTPTFIKELFDVNGVVASEFGLLDPNREATPQLKIELRKAIVAAWNRLKREGT
PKIVFTAHDDNIEDKWTARFLQETLDVPSEFVPLHTLLVDEDGVYTPSGERVDILYRQTYPIEHLVDDRAPDGDLIGIRL
LELDQQKYVSLLNPLSAFLMQSKGVQALIWELYETETLFDQAERDIIGTYFLPTYLEDTPFFGESDYVKKPAFGREGDSV
ILYTKDGKPFHRETLQTYAEETAVYQQFIELPTRMTTTVKGRMETHYMIGCFCLNGHPSALGVRAGSMITNNQSYYLAVG
TPIQKETNS

Sequences:

>Translated_409_residues
MTRDELYSQIPYFWPDLNDEEYALYDCFEMTASQVASIREATEAVDSIFRKTNQLLRTLPDETLRLLGYPEASLPFLREK
TIPQETIIGRYDWIMKDDQLKLMEFNADTPTFIKELFDVNGVVASEFGLLDPNREATPQLKIELRKAIVAAWNRLKREGT
PKIVFTAHDDNIEDKWTARFLQETLDVPSEFVPLHTLLVDEDGVYTPSGERVDILYRQTYPIEHLVDDRAPDGDLIGIRL
LELDQQKYVSLLNPLSAFLMQSKGVQALIWELYETETLFDQAERDIIGTYFLPTYLEDTPFFGESDYVKKPAFGREGDSV
ILYTKDGKPFHRETLQTYAEETAVYQQFIELPTRMTTTVKGRMETHYMIGCFCLNGHPSALGVRAGSMITNNQSYYLAVG
TPIQKETNS
>Mature_408_residues
TRDELYSQIPYFWPDLNDEEYALYDCFEMTASQVASIREATEAVDSIFRKTNQLLRTLPDETLRLLGYPEASLPFLREKT
IPQETIIGRYDWIMKDDQLKLMEFNADTPTFIKELFDVNGVVASEFGLLDPNREATPQLKIELRKAIVAAWNRLKREGTP
KIVFTAHDDNIEDKWTARFLQETLDVPSEFVPLHTLLVDEDGVYTPSGERVDILYRQTYPIEHLVDDRAPDGDLIGIRLL
ELDQQKYVSLLNPLSAFLMQSKGVQALIWELYETETLFDQAERDIIGTYFLPTYLEDTPFFGESDYVKKPAFGREGDSVI
LYTKDGKPFHRETLQTYAEETAVYQQFIELPTRMTTTVKGRMETHYMIGCFCLNGHPSALGVRAGSMITNNQSYYLAVGT
PIQKETNS

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli yjfC [H]

Homologues:

Organism=Escherichia coli, GI1789416, Length=377, Percent_Identity=22.5464190981432, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: 6.3.1.8

Molecular weight: Translated: 47036; Mature: 46905

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDELYSQIPYFWPDLNDEEYALYDCFEMTASQVASIREATEAVDSIFRKTNQLLRTLP
CCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DETLRLLGYPEASLPFLREKTIPQETIIGRYDWIMKDDQLKLMEFNADTPTFIKELFDVN
HHHHHHHCCCCCCCCHHHHCCCCHHHHCCCHHEEEECCCEEEEEECCCCHHHHHHHHHCC
GVVASEFGLLDPNREATPQLKIELRKAIVAAWNRLKREGTPKIVFTAHDDNIEDKWTARF
CEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
LQETLDVPSEFVPLHTLLVDEDGVYTPSGERVDILYRQTYPIEHLVDDRAPDGDLIGIRL
HHHHHCCCHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCEEEEEE
LELDQQKYVSLLNPLSAFLMQSKGVQALIWELYETETLFDQAERDIIGTYFLPTYLEDTP
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCC
FFGESDYVKKPAFGREGDSVILYTKDGKPFHRETLQTYAEETAVYQQFIELPTRMTTTVK
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEE
GRMETHYMIGCFCLNGHPSALGVRAGSMITNNQSYYLAVGTPIQKETNS
CCCCEEEEEEEEEECCCCCEEEEECCCEEECCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TRDELYSQIPYFWPDLNDEEYALYDCFEMTASQVASIREATEAVDSIFRKTNQLLRTLP
CHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DETLRLLGYPEASLPFLREKTIPQETIIGRYDWIMKDDQLKLMEFNADTPTFIKELFDVN
HHHHHHHCCCCCCCCHHHHCCCCHHHHCCCHHEEEECCCEEEEEECCCCHHHHHHHHHCC
GVVASEFGLLDPNREATPQLKIELRKAIVAAWNRLKREGTPKIVFTAHDDNIEDKWTARF
CEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHH
LQETLDVPSEFVPLHTLLVDEDGVYTPSGERVDILYRQTYPIEHLVDDRAPDGDLIGIRL
HHHHHCCCHHHCCEEEEEECCCCCCCCCCCEEEEEEECCCCHHHHHCCCCCCCCEEEEEE
LELDQQKYVSLLNPLSAFLMQSKGVQALIWELYETETLFDQAERDIIGTYFLPTYLEDTP
EECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHCCCCC
FFGESDYVKKPAFGREGDSVILYTKDGKPFHRETLQTYAEETAVYQQFIELPTRMTTTVK
CCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEE
GRMETHYMIGCFCLNGHPSALGVRAGSMITNNQSYYLAVGTPIQKETNS
CCCCEEEEEEEEEECCCCCEEEEECCCEEECCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gamma-L-glutamyl-L-cysteinyl-glycine; spermidine; ATP

Specific reaction: gamma-L-glutamyl-L-cysteinyl-glycine + spermidine + ATP = N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]