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The map label for this gene is gltT [H]

Identifier: 229917115

GI number: 229917115

Start: 1360050

End: 1361327

Strand: Reverse

Name: gltT [H]

Synonym: EAT1b_1389

Alternate gene names: 229917115

Gene position: 1361327-1360050 (Counterclockwise)

Preceding gene: 229917116

Following gene: 229917114

Centisome position: 45.38

GC content: 49.69

Gene sequence:

>1278_bases
ATGAGCGAAACGAAAAAAATCTTAATCGGTCTCGCGCTAGGTGTTGTTGTTGGTCTCGGATTGGCGGGCTTGCCGGATAA
TGTTTATGAGATTGTGAATCCGTATATTTTATCTCCGGTCGGAACGATTTTTTTGAATTTAATTAAAATGTTAGTCGTAC
CGATTGTCTTCTTCTCAATTATTTTAGGCGTCATGGGTCTCGGGGACCCGAAAGAACTAGGTCGAGTCGGGTCGAAGTCG
ATTCTGTTCTTCTTAGCGACGACAGCGCTCGCAATCATGTTAGCGATGGGTGTTGCATCCGTCTTACAGCCAGGGGAGCG
TGGGGACTTCCAAACGACAGGTCTTGAATTTGAATCGACAGCGACCGAGGAATCGTCTAATATCGTACAGACATTCGTCA
ATATGATTCCAACGAATCCGATTACGGCGCTCGCTGAAGGAAACATGCTTCAAATCATCGTCTTTGCGGCATTGATTGGG
TTTGCGCTGATTGCACTCGGGGACAAGGCGAAGACGTGGCGTCAATTCGTCAAACAAGGCGATCGCATCATGATGTACTT
GATTCATCTTGTGATGAAGCTCGCTCCATACGGCGCATTCGCATTGATTGCTTCGGCAATCGGAGGCATCGGTCTCGACG
CGGTTGTCGCTATGGCATGGTATATGATTGCCGTTGTCTTGACGTTATTCTTACATGCAACGATTGTCTACGGGGCAGCG
GTTTACTTCATCGGGAAGATGAGCCCAGTGTTCTTCTTCAAGAACTTCTATGAGGCGATGACGTTCGCGTTCTCGACCTC
TTCGTCAAACGCGACGCTTCCAGTATCGATGGAAGTCGCACAGAAAAAACTCGGTGTGCCGCGTTCGATTTCGTCGTTCG
TACAGCCACTCGGAGCAACGGTCAACATGGACGGAACGGCAATCATGCAAGGGGTTGCGACGATTTTCATCGCGCAAGTG
TTTGCAGCTGATCTAACGATGACTGAGTTGTTGACAGTCGTCATCACGGCGGTCCTCGCTTCGATCGGTACAGCGGGTGT
ACCGGGAGTCGGACTTGTCATGCTTGCGCTCGTCCTTCAGTCAGTCAACTTACCGGTAGAGGGAATCGCCCTCATTATCG
GGGTCGACCGTATTCTCGATATGCTTCACACATCTGTCAACATCACGGGGGATGCGGCATGTGCGGTTGTCGTTTCGAAA
TTGGAAGAAAAACATCTACCACCAGTCGACGAAGACTCGTGGGATGAAGCAGGTGCGACTTCTTCATCATCGCTATGA

Upstream 100 bases:

>100_bases
CAAGAGATTTTGTAACTGAAGAGATATTCAGTTGTAATTGTATGAAAATTCAGTATTATGATATACTGACTACATTGTGA
AAAAAAGAAAGGGTGATACG

Downstream 100 bases:

>100_bases
TATAGTGAAGGACCATGACGACATGTCATGGTCCTTATTTTGTGAAGAGAAAGAGTGATGATTATGGAATGGGAACTGCT
CCCGCTCCCTTTATTTTTGA

Product: sodium:dicarboxylate symporter

Products: L-aspartate [Cytoplasm]; Proton [Cytoplasm]; L-glutamate [Cytoplasm] [C]

Alternate protein names: Glutamate-aspartate carrier protein [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MSETKKILIGLALGVVVGLGLAGLPDNVYEIVNPYILSPVGTIFLNLIKMLVVPIVFFSIILGVMGLGDPKELGRVGSKS
ILFFLATTALAIMLAMGVASVLQPGERGDFQTTGLEFESTATEESSNIVQTFVNMIPTNPITALAEGNMLQIIVFAALIG
FALIALGDKAKTWRQFVKQGDRIMMYLIHLVMKLAPYGAFALIASAIGGIGLDAVVAMAWYMIAVVLTLFLHATIVYGAA
VYFIGKMSPVFFFKNFYEAMTFAFSTSSSNATLPVSMEVAQKKLGVPRSISSFVQPLGATVNMDGTAIMQGVATIFIAQV
FAADLTMTELLTVVITAVLASIGTAGVPGVGLVMLALVLQSVNLPVEGIALIIGVDRILDMLHTSVNITGDAACAVVVSK
LEEKHLPPVDEDSWDEAGATSSSSL

Sequences:

>Translated_425_residues
MSETKKILIGLALGVVVGLGLAGLPDNVYEIVNPYILSPVGTIFLNLIKMLVVPIVFFSIILGVMGLGDPKELGRVGSKS
ILFFLATTALAIMLAMGVASVLQPGERGDFQTTGLEFESTATEESSNIVQTFVNMIPTNPITALAEGNMLQIIVFAALIG
FALIALGDKAKTWRQFVKQGDRIMMYLIHLVMKLAPYGAFALIASAIGGIGLDAVVAMAWYMIAVVLTLFLHATIVYGAA
VYFIGKMSPVFFFKNFYEAMTFAFSTSSSNATLPVSMEVAQKKLGVPRSISSFVQPLGATVNMDGTAIMQGVATIFIAQV
FAADLTMTELLTVVITAVLASIGTAGVPGVGLVMLALVLQSVNLPVEGIALIIGVDRILDMLHTSVNITGDAACAVVVSK
LEEKHLPPVDEDSWDEAGATSSSSL
>Mature_424_residues
SETKKILIGLALGVVVGLGLAGLPDNVYEIVNPYILSPVGTIFLNLIKMLVVPIVFFSIILGVMGLGDPKELGRVGSKSI
LFFLATTALAIMLAMGVASVLQPGERGDFQTTGLEFESTATEESSNIVQTFVNMIPTNPITALAEGNMLQIIVFAALIGF
ALIALGDKAKTWRQFVKQGDRIMMYLIHLVMKLAPYGAFALIASAIGGIGLDAVVAMAWYMIAVVLTLFLHATIVYGAAV
YFIGKMSPVFFFKNFYEAMTFAFSTSSSNATLPVSMEVAQKKLGVPRSISSFVQPLGATVNMDGTAIMQGVATIFIAQVF
AADLTMTELLTVVITAVLASIGTAGVPGVGLVMLALVLQSVNLPVEGIALIIGVDRILDMLHTSVNITGDAACAVVVSKL
EEKHLPPVDEDSWDEAGATSSSSL

Specific function: This carrier protein is part of the Na(+)-dependent, binding-protein-independent glutamate-aspartate transport system [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI40254478, Length=456, Percent_Identity=31.359649122807, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI169790839, Length=426, Percent_Identity=29.81220657277, Blast_Score=185, Evalue=8e-47,
Organism=Homo sapiens, GI4827012, Length=281, Percent_Identity=38.4341637010676, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI194239697, Length=429, Percent_Identity=29.6037296037296, Blast_Score=178, Evalue=1e-44,
Organism=Homo sapiens, GI21314632, Length=403, Percent_Identity=30.5210918114144, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5032093, Length=373, Percent_Identity=33.7801608579088, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI66773030, Length=454, Percent_Identity=29.295154185022, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI223468566, Length=277, Percent_Identity=35.7400722021661, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI223468564, Length=248, Percent_Identity=36.2903225806452, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI262359914, Length=349, Percent_Identity=25.7879656160458, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI301601644, Length=181, Percent_Identity=39.2265193370166, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1790514, Length=411, Percent_Identity=37.4695863746959, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1789947, Length=397, Percent_Identity=31.4861460957179, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1788024, Length=431, Percent_Identity=29.2343387470998, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789473, Length=379, Percent_Identity=26.3852242744063, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=32.7510917030568, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=32.7510917030568, Blast_Score=221, Evalue=7e-58,
Organism=Caenorhabditis elegans, GI193206505, Length=440, Percent_Identity=34.3181818181818, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI17537407, Length=441, Percent_Identity=34.0136054421769, Blast_Score=201, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI71996953, Length=457, Percent_Identity=31.7286652078775, Blast_Score=194, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI193206654, Length=455, Percent_Identity=31.6483516483516, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17541374, Length=413, Percent_Identity=30.9927360774818, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24583025, Length=416, Percent_Identity=30.2884615384615, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17137668, Length=416, Percent_Identity=30.2884615384615, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24583023, Length=416, Percent_Identity=30.2884615384615, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI281360483, Length=474, Percent_Identity=27.6371308016878, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17137666, Length=474, Percent_Identity=27.6371308016878, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281360481, Length=464, Percent_Identity=27.801724137931, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 44864; Mature: 44733

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCC
>Mature Secondary Structure 
SETKKILIGLALGVVVGLGLAGLPDNVYEIVNPYILSPVGTIFLNLIKMLVVPIVFFSI
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGVMGLGDPKELGRVGSKSILFFLATTALAIMLAMGVASVLQPGERGDFQTTGLEFEST
HHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
ATEESSNIVQTFVNMIPTNPITALAEGNMLQIIVFAALIGFALIALGDKAKTWRQFVKQG
CCHHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
DRIMMYLIHLVMKLAPYGAFALIASAIGGIGLDAVVAMAWYMIAVVLTLFLHATIVYGAA
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYFIGKMSPVFFFKNFYEAMTFAFSTSSSNATLPVSMEVAQKKLGVPRSISSFVQPLGAT
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHCCC
VNMDGTAIMQGVATIFIAQVFAADLTMTELLTVVITAVLASIGTAGVPGVGLVMLALVLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
SVNLPVEGIALIIGVDRILDMLHTSVNITGDAACAVVVSKLEEKHLPPVDEDSWDEAGAT
HCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SSSSL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-aspartate [Periplasm]; Proton [Periplasm]; L-glutamate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-aspartate [Periplasm] = Proton [Cytoplasm] + L-aspartate [Cytoplasm] Proton [Periplasm] + L-glutamate [Periplasm] = Proton [Cytoplasm] + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]