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The map label for this gene is capI [H]

Identifier: 229917103

GI number: 229917103

Start: 1347585

End: 1348613

Strand: Reverse

Name: capI [H]

Synonym: EAT1b_1377

Alternate gene names: 229917103

Gene position: 1348613-1347585 (Counterclockwise)

Preceding gene: 229917104

Following gene: 229917102

Centisome position: 44.96

GC content: 46.65

Gene sequence:

>1029_bases
ATGAAAAAAACAGTTCTCGTGACCGGAGTGGCCGGGTTCATTGGTTTTCATCTAGCTCGACGGTTGCTAAAAGAAGGACA
TCATGTCATCGGGATCGATGTGGTCAATGATTATTACGATCCGAGATTAAAAGAATCAAGACTTCATGTGTTGACGCATC
CTCGTTTCAAACTCTATCGTGATGCCCTCGAAAATAAGATGGCAATCACGCGTGTCTTCGAAAAAAACCAACCCCAAGTC
GTGGTCAATTTGGCGGCTCAGGCCGGTGTACGCTACAGTCTAGAAAATCCAGATGCTTATATTCAATCCAATATCGTTGG
TTTCTTGAACATCTTGGAGGCTTGTCGCTTCTATCCAGTAGAGCAATTGATTTATGCCTCATCTAGCTCGGTTTACGGAT
CAAATCAGGCGATGCCATTTTCCGAACAACACCCGGTCGATCATCCGCTATCCTTGTATGCGGCTTCGAAGAAGGCAAAT
GAGTTGATGGCTCATACGTACAGCCATTTGTTCGGTCTAAAAACGACGGGGTTGCGATTCTTTAGCGTCTATGGGCCATG
GGGGCGTCCCGATATGGCACTTTACAAATTTACGGAGGCGATTTTAAAAGGCGAGCCAATCGACGTCTACAACTACGGTG
AGATGGAACGAGATTTCACATACATCGACGACATCATCGAGTCGATTGTTCGACTTATGGATATCGCACCGATGGCAGAC
GAGGAGTTTGATTATGAACATCCTCTACCGGATCGGAGTGACGTTCCGTTTCGTGTCTATAACATTGGAAATCAGCAACC
GGTATCCTTGATGGATTTTATTCGGACCATCGAAGAGCGAGTCGGAAAGAAAGCCATCCTCAGCTTGTTACCACTCCAGG
CGGGTGATGTACCCGCGAGTTTTGCAGACTCCTCCTCTTTGTATGAATCGATTGACTTTCAACCACAGACGTCGATTCGC
GATGGCGTAAACGCGTTTATCGACTGGTACATCGCCTATCATCAAGTGTTGAAGGAGGGCATCGAGTAA

Upstream 100 bases:

>100_bases
ACAACGAACTGCCAGAAGAAGTGATGGAAAGTATTTTTGCGCCACAAATCGAACCGGTACGCATGCGAGTCAACGAGTCA
ACGCAAAGGAGGAAGTCGTT

Downstream 100 bases:

>100_bases
TGGGGATTTTACAGGATCGGATTTATGCACACTCACCGATTCGACTCCAAAATTGGCTGGTTTCCTTATATGGAATCAAG
TTATATCGGGAGCGATACGG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKKTVLVTGVAGFIGFHLARRLLKEGHHVIGIDVVNDYYDPRLKESRLHVLTHPRFKLYRDALENKMAITRVFEKNQPQV
VVNLAAQAGVRYSLENPDAYIQSNIVGFLNILEACRFYPVEQLIYASSSSVYGSNQAMPFSEQHPVDHPLSLYAASKKAN
ELMAHTYSHLFGLKTTGLRFFSVYGPWGRPDMALYKFTEAILKGEPIDVYNYGEMERDFTYIDDIIESIVRLMDIAPMAD
EEFDYEHPLPDRSDVPFRVYNIGNQQPVSLMDFIRTIEERVGKKAILSLLPLQAGDVPASFADSSSLYESIDFQPQTSIR
DGVNAFIDWYIAYHQVLKEGIE

Sequences:

>Translated_342_residues
MKKTVLVTGVAGFIGFHLARRLLKEGHHVIGIDVVNDYYDPRLKESRLHVLTHPRFKLYRDALENKMAITRVFEKNQPQV
VVNLAAQAGVRYSLENPDAYIQSNIVGFLNILEACRFYPVEQLIYASSSSVYGSNQAMPFSEQHPVDHPLSLYAASKKAN
ELMAHTYSHLFGLKTTGLRFFSVYGPWGRPDMALYKFTEAILKGEPIDVYNYGEMERDFTYIDDIIESIVRLMDIAPMAD
EEFDYEHPLPDRSDVPFRVYNIGNQQPVSLMDFIRTIEERVGKKAILSLLPLQAGDVPASFADSSSLYESIDFQPQTSIR
DGVNAFIDWYIAYHQVLKEGIE
>Mature_342_residues
MKKTVLVTGVAGFIGFHLARRLLKEGHHVIGIDVVNDYYDPRLKESRLHVLTHPRFKLYRDALENKMAITRVFEKNQPQV
VVNLAAQAGVRYSLENPDAYIQSNIVGFLNILEACRFYPVEQLIYASSSSVYGSNQAMPFSEQHPVDHPLSLYAASKKAN
ELMAHTYSHLFGLKTTGLRFFSVYGPWGRPDMALYKFTEAILKGEPIDVYNYGEMERDFTYIDDIIESIVRLMDIAPMAD
EEFDYEHPLPDRSDVPFRVYNIGNQQPVSLMDFIRTIEERVGKKAILSLLPLQAGDVPASFADSSSLYESIDFQPQTSIR
DGVNAFIDWYIAYHQVLKEGIE

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=26.8436578171091, Blast_Score=108, Evalue=5e-24,
Organism=Homo sapiens, GI56237023, Length=329, Percent_Identity=26.4437689969605, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI56118217, Length=329, Percent_Identity=26.4437689969605, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI189083684, Length=329, Percent_Identity=26.4437689969605, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI7657641, Length=338, Percent_Identity=23.6686390532544, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI48994969, Length=361, Percent_Identity=24.9307479224377, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=23.943661971831, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786974, Length=327, Percent_Identity=25.9938837920489, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788589, Length=255, Percent_Identity=30.1960784313725, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788366, Length=214, Percent_Identity=30.8411214953271, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=341, Percent_Identity=26.6862170087977, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=349, Percent_Identity=25.7879656160458, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=22.3495702005731, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=22.5071225071225, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=349, Percent_Identity=22.3495702005731, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI133901786, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=244, Percent_Identity=25.8196721311475, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=244, Percent_Identity=25.4098360655738, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=345, Percent_Identity=25.2173913043478, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=26.6272189349112, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=25.3687315634218, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24158427, Length=248, Percent_Identity=24.5967741935484, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38974; Mature: 38974

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTVLVTGVAGFIGFHLARRLLKEGHHVIGIDVVNDYYDPRLKESRLHVLTHPRFKLYR
CCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCHHHHHH
DALENKMAITRVFEKNQPQVVVNLAAQAGVRYSLENPDAYIQSNIVGFLNILEACRFYPV
HHHHHHHHHHHHHHCCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCH
EQLIYASSSSVYGSNQAMPFSEQHPVDHPLSLYAASKKANELMAHTYSHLFGLKTTGLRF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEE
FSVYGPWGRPDMALYKFTEAILKGEPIDVYNYGEMERDFTYIDDIIESIVRLMDIAPMAD
EEEECCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
EEFDYEHPLPDRSDVPFRVYNIGNQQPVSLMDFIRTIEERVGKKAILSLLPLQAGDVPAS
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FADSSSLYESIDFQPQTSIRDGVNAFIDWYIAYHQVLKEGIE
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKTVLVTGVAGFIGFHLARRLLKEGHHVIGIDVVNDYYDPRLKESRLHVLTHPRFKLYR
CCCEEEEEHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEECCHHHHHH
DALENKMAITRVFEKNQPQVVVNLAAQAGVRYSLENPDAYIQSNIVGFLNILEACRFYPV
HHHHHHHHHHHHHHCCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCH
EQLIYASSSSVYGSNQAMPFSEQHPVDHPLSLYAASKKANELMAHTYSHLFGLKTTGLRF
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCEEE
FSVYGPWGRPDMALYKFTEAILKGEPIDVYNYGEMERDFTYIDDIIESIVRLMDIAPMAD
EEEECCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
EEFDYEHPLPDRSDVPFRVYNIGNQQPVSLMDFIRTIEERVGKKAILSLLPLQAGDVPAS
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
FADSSSLYESIDFQPQTSIRDGVNAFIDWYIAYHQVLKEGIE
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]