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The map label for this gene is pyrG [H]

Identifier: 229917083

GI number: 229917083

Start: 1326625

End: 1328220

Strand: Reverse

Name: pyrG [H]

Synonym: EAT1b_1357

Alternate gene names: 229917083

Gene position: 1328220-1326625 (Counterclockwise)

Preceding gene: 229917084

Following gene: 229917082

Centisome position: 44.28

GC content: 49.56

Gene sequence:

>1596_bases
ATGACAAAGTACATTTTCGTAACAGGCGGGGTAGTATCTTCGCTCGGTAAAGGGATCACGGCAGCTTCGCTCGCGCGTCT
ACTCAAAAATCGAGGACTCAACGTCACGATTCAGAAGTTTGACCCATACATCAACATTGACCCAGGTACGATGAGCCCGT
ATCAACACGGAGAAGTATTCGTCACAGATGATGGCGCTGAAACTGACCTTGACCTCGGACACTATGAGCGCTTTATCGAC
ATCAACTTGTCGCAAAACGCGAACGTCACATCAGGTCGCATTTATTCGACTGTCTTGAAAAAAGAGCGTCGTGGAGACTA
TAACGGTGGAACGGTTCAGGTCATTCCACATATCACGAATGAGATCAAAGACCGTGTCTTCCGCGCAGGACGTGAGACGA
ACGCAGATGTCGTCATCACAGAGATTGGTGGAACAGTCGGAGATATCGAATCTCTTCCGTTCATCGAAGCCATTCGCCAA
GTAAAAAACGATATTGGTAAAGAGAACGTCATGTACGTTCACTGTACGCTCGTTCCTTACCTTCGTGCCGCAGGTGAGAT
GAAGACGAAACCGACACAACACAGCGTAAAAGAACTTCGCAGCTACGGGATTCAACCAGACATCATCGTTCTCCGTACGG
AGCACGATATCCCAGAAGACATGCGTGAGAAAATCGCCCTCTTCTGTGATACGCGCAAAGAAGCAGTCATCGAAGCGCAA
GACGCATCGACACTTTACGAAGTACCGCTCAACCTTCAAAAACAAGGGATGGACCAACTCGTCAACGACTACTTCGGTTT
CAACACACCAGCTGCCGACATGACGGCGTGGAAAGAACTCGTTCACACGGTCACACACCTTGAGAAGAAAGTGAAAATCG
CGCTCGTCGGGAAATATGTCGAGTTGCGTGATGCCTATATCTCAGTCGCAGAGGCGCTCAAGCATGCCGGTTATGCGTTC
AACGCGGACATCGACATCGACTGGATCAACGCAGAAGACGTGACAGCTGAAAACGTTTCGGACATCCTCGGTGATGCTGA
CGGAATCCTCGTCCCAGGTGGGTTCGGTGAGCGTGGAATTGAAGGGAAGATCGAAGCAACACGTTTTGCTCGTGAAAACA
ATGTCCCATTCTTCGGAATCTGTCTCGGGATGCAACTTGCGACGGTCGAGTTTGCCCGTAACGTCTTGAAGTTGGACGGC
GCCCATACGGCTGAAATCGATCCGTCGACACCGTATCCGATCATCGACCTTCTTCCAGAGCAAAAAGATATCGAAGACCT
TGGCGGCACACTTCGTCTTGGACTTTATCCATGTAAACTCGAAAAAGGGACGAAAGCGCATGCGGCATACGATCAAGAAC
TCGTCTACGAGCGTCATCGTCACCGTTATGAGTTCAACAACGAATACCGTGAAGCGTTTGAAGCAGAAGGCTTCAAGTTC
TCAGGTACGAGCCCAGACGGTCGTCTCGTAGAAATCATTGAGATTCCAGAACACAAATGGTTCGTGGCATCACAGTTCCA
CCCAGAATTGATTTCGCGTCCAGAACGTCCACAACGTCTATTCCATGACTTCATCCAAGCGTCACTTGGAGAATAA

Upstream 100 bases:

>100_bases
GTAACGATGCAGCAAAAGTGTCCTCCATTTTCGGGGGCACTTTTGCTTTTTTTGTTTTTTCGTTTCCCGATGGTTGGTAT
TCAAGAGAGGAGAAAGAAAC

Downstream 100 bases:

>100_bases
GTGATTGAAAGCCACGATCTGAAATCGATCGTGGCTTTTCTACGTCAAAAGCATGAGAGGGAAGGTGCCCTCGGCAGGAT
ACATCCCCTTCACTTTCTGG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 531; Mature: 530

Protein sequence:

>531_residues
MTKYIFVTGGVVSSLGKGITAASLARLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLSQNANVTSGRIYSTVLKKERRGDYNGGTVQVIPHITNEIKDRVFRAGRETNADVVITEIGGTVGDIESLPFIEAIRQ
VKNDIGKENVMYVHCTLVPYLRAAGEMKTKPTQHSVKELRSYGIQPDIIVLRTEHDIPEDMREKIALFCDTRKEAVIEAQ
DASTLYEVPLNLQKQGMDQLVNDYFGFNTPAADMTAWKELVHTVTHLEKKVKIALVGKYVELRDAYISVAEALKHAGYAF
NADIDIDWINAEDVTAENVSDILGDADGILVPGGFGERGIEGKIEATRFARENNVPFFGICLGMQLATVEFARNVLKLDG
AHTAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLEKGTKAHAAYDQELVYERHRHRYEFNNEYREAFEAEGFKF
SGTSPDGRLVEIIEIPEHKWFVASQFHPELISRPERPQRLFHDFIQASLGE

Sequences:

>Translated_531_residues
MTKYIFVTGGVVSSLGKGITAASLARLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFID
INLSQNANVTSGRIYSTVLKKERRGDYNGGTVQVIPHITNEIKDRVFRAGRETNADVVITEIGGTVGDIESLPFIEAIRQ
VKNDIGKENVMYVHCTLVPYLRAAGEMKTKPTQHSVKELRSYGIQPDIIVLRTEHDIPEDMREKIALFCDTRKEAVIEAQ
DASTLYEVPLNLQKQGMDQLVNDYFGFNTPAADMTAWKELVHTVTHLEKKVKIALVGKYVELRDAYISVAEALKHAGYAF
NADIDIDWINAEDVTAENVSDILGDADGILVPGGFGERGIEGKIEATRFARENNVPFFGICLGMQLATVEFARNVLKLDG
AHTAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLEKGTKAHAAYDQELVYERHRHRYEFNNEYREAFEAEGFKF
SGTSPDGRLVEIIEIPEHKWFVASQFHPELISRPERPQRLFHDFIQASLGE
>Mature_530_residues
TKYIFVTGGVVSSLGKGITAASLARLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDI
NLSQNANVTSGRIYSTVLKKERRGDYNGGTVQVIPHITNEIKDRVFRAGRETNADVVITEIGGTVGDIESLPFIEAIRQV
KNDIGKENVMYVHCTLVPYLRAAGEMKTKPTQHSVKELRSYGIQPDIIVLRTEHDIPEDMREKIALFCDTRKEAVIEAQD
ASTLYEVPLNLQKQGMDQLVNDYFGFNTPAADMTAWKELVHTVTHLEKKVKIALVGKYVELRDAYISVAEALKHAGYAFN
ADIDIDWINAEDVTAENVSDILGDADGILVPGGFGERGIEGKIEATRFARENNVPFFGICLGMQLATVEFARNVLKLDGA
HTAEIDPSTPYPIIDLLPEQKDIEDLGGTLRLGLYPCKLEKGTKAHAAYDQELVYERHRHRYEFNNEYREAFEAEGFKFS
GTSPDGRLVEIIEIPEHKWFVASQFHPELISRPERPQRLFHDFIQASLGE

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=555, Percent_Identity=45.5855855855856, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI28559085, Length=553, Percent_Identity=44.6654611211573, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559083, Length=553, Percent_Identity=44.6654611211573, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI221316689, Length=553, Percent_Identity=44.6654611211573, Blast_Score=473, Evalue=1e-133,
Organism=Escherichia coli, GI1789142, Length=537, Percent_Identity=54.5623836126629, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI25148299, Length=602, Percent_Identity=38.3720930232558, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6319432, Length=565, Percent_Identity=42.6548672566372, Blast_Score=458, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322563, Length=562, Percent_Identity=43.0604982206406, Blast_Score=455, Evalue=1e-129,
Organism=Drosophila melanogaster, GI24664469, Length=548, Percent_Identity=45.6204379562044, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI21357815, Length=493, Percent_Identity=43.4077079107505, Blast_Score=404, Evalue=1e-113,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 59535; Mature: 59404

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYIFVTGGVVSSLGKGITAASLARLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVF
CCEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLSQNANVTSGRIYSTVLKKERRGDYNGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHH
EIKDRVFRAGRETNADVVITEIGGTVGDIESLPFIEAIRQVKNDIGKENVMYVHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LRAAGEMKTKPTQHSVKELRSYGIQPDIIVLRTEHDIPEDMREKIALFCDTRKEAVIEAQ
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHEEEEC
DASTLYEVPLNLQKQGMDQLVNDYFGFNTPAADMTAWKELVHTVTHLEKKVKIALVGKYV
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEHHH
ELRDAYISVAEALKHAGYAFNADIDIDWINAEDVTAENVSDILGDADGILVPGGFGERGI
HHHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKIEATRFARENNVPFFGICLGMQLATVEFARNVLKLDGAHTAEIDPSTPYPIIDLLPE
CCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCC
QKDIEDLGGTLRLGLYPCKLEKGTKAHAAYDQELVYERHRHRYEFNNEYREAFEAEGFKF
CCCHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
SGTSPDGRLVEIIEIPEHKWFVASQFHPELISRPERPQRLFHDFIQASLGE
CCCCCCCCEEEEEECCCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKYIFVTGGVVSSLGKGITAASLARLLKNRGLNVTIQKFDPYINIDPGTMSPYQHGEVF
CEEEEEECHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCCCCCCCEEE
VTDDGAETDLDLGHYERFIDINLSQNANVTSGRIYSTVLKKERRGDYNGGTVQVIPHITN
EECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECHHHH
EIKDRVFRAGRETNADVVITEIGGTVGDIESLPFIEAIRQVKNDIGKENVMYVHCTLVPY
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHH
LRAAGEMKTKPTQHSVKELRSYGIQPDIIVLRTEHDIPEDMREKIALFCDTRKEAVIEAQ
HHHCCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCHHHEEEEC
DASTLYEVPLNLQKQGMDQLVNDYFGFNTPAADMTAWKELVHTVTHLEKKVKIALVGKYV
CCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEHHH
ELRDAYISVAEALKHAGYAFNADIDIDWINAEDVTAENVSDILGDADGILVPGGFGERGI
HHHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCCCCC
EGKIEATRFARENNVPFFGICLGMQLATVEFARNVLKLDGAHTAEIDPSTPYPIIDLLPE
CCCCHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCC
QKDIEDLGGTLRLGLYPCKLEKGTKAHAAYDQELVYERHRHRYEFNNEYREAFEAEGFKF
CCCHHHCCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
SGTSPDGRLVEIIEIPEHKWFVASQFHPELISRPERPQRLFHDFIQASLGE
CCCCCCCCEEEEEECCCCCEEEECCCCHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA