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The map label for this gene is purR [H]

Identifier: 229916997

GI number: 229916997

Start: 1246416

End: 1247435

Strand: Direct

Name: purR [H]

Synonym: EAT1b_1271

Alternate gene names: 229916997

Gene position: 1246416-1247435 (Clockwise)

Preceding gene: 229916991

Following gene: 229916999

Centisome position: 41.55

GC content: 43.24

Gene sequence:

>1020_bases
ATGATGGCCACATTAAAAGACGTTTCTAAAGCATCCGGTTTTTCCATCACAACGGTATCTCGAGCCTTAAACGGCTACGA
TGATGTAAACAAAGAGACACGGGACAAGATTTTAAATGTCGCTAAAGAACTTGGTTATAGCCCGAACATTTTGGCACGCA
GTTTGGTCAAAAAGCAATCCAAGACAATCGGCTTTCTCGTCACTGATTTAAAACGCGAGAGTATTAAAGATAACTTTATG
TTCGAGACTTTATGCGGTGTATCAGATGAATTGTCTGACCTTGATTACGAGTTCGTTCTTTTATCGACTACGACCTCAAA
GCAAAAAAATAAAACGTACAGTCAAATGTGCGCAGAACGTCAACTAGACGGGGTTGTTATTCAAGGATTAAAACGAGACG
ACCCCTACTTACTCGAGGCAATCGAAAGCACAGTACCTTGCGTACTGGTCGACATTCCTGTCGAAGGGATCAATACAGGT
TATGTGACCTCAAATCAGCTCGAGAGCGCTAAACAAGCCGTTCGTTATCTCATCCGACTCGGACACCGACACATTGCATT
TATGAACGGCGCACCACACGCCTATGTTTCGACTGTACGATATGATGCCTATCGACAAGTGCTACACGATCACGAAATCG
ACTTCCAAGAAGAATATGTGTTAAACGGAGATTTTGAAGAAATCGCCTCAAAAGATGCAGCACTTCCTTTTTTGTTGAAT
CACCCTGAAGTGACAGCGTTTTTCTGTGCATCCGATGTCATGGCACTTGGTGTCCTCCAGGCAACGAGAGAACTCGGCTT
AAACGTTCCAGAAGATCTATCAATCATCGGATTTGACAATATCTTATTGTCTCAGTACGTATCCCCACCGTTAACCACTG
TTGGACAACAACCTTATGAAATGGGGCGAAAAGCCGCGTCGATGATTGTCAATATTGTAGAAGGTCAAGATGTGCCTCAC
GAAGCTTTCGTCAATAATCAGTTAATCCTGCGAGAATCAGTCACAAAAAATCGGCGATGA

Upstream 100 bases:

>100_bases
TTCAATTCAAGGTTTACAAGATATTCATATACAGACTAAACTAGATGTACCTATAAATTTTACTTGATTTACTCACAACG
TTTTCATAAAGGAGTATTGT

Downstream 100 bases:

>100_bases
TTAGTCATCGCCGATTTTTTGTATCGTCATAAATGCAAAGATACCGACAGTCGCCCAATATAAAACAGAGAGCATAGGCA
CATTGATTGGCAATAACTCA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MMATLKDVSKASGFSITTVSRALNGYDDVNKETRDKILNVAKELGYSPNILARSLVKKQSKTIGFLVTDLKRESIKDNFM
FETLCGVSDELSDLDYEFVLLSTTTSKQKNKTYSQMCAERQLDGVVIQGLKRDDPYLLEAIESTVPCVLVDIPVEGINTG
YVTSNQLESAKQAVRYLIRLGHRHIAFMNGAPHAYVSTVRYDAYRQVLHDHEIDFQEEYVLNGDFEEIASKDAALPFLLN
HPEVTAFFCASDVMALGVLQATRELGLNVPEDLSIIGFDNILLSQYVSPPLTTVGQQPYEMGRKAASMIVNIVEGQDVPH
EAFVNNQLILRESVTKNRR

Sequences:

>Translated_339_residues
MMATLKDVSKASGFSITTVSRALNGYDDVNKETRDKILNVAKELGYSPNILARSLVKKQSKTIGFLVTDLKRESIKDNFM
FETLCGVSDELSDLDYEFVLLSTTTSKQKNKTYSQMCAERQLDGVVIQGLKRDDPYLLEAIESTVPCVLVDIPVEGINTG
YVTSNQLESAKQAVRYLIRLGHRHIAFMNGAPHAYVSTVRYDAYRQVLHDHEIDFQEEYVLNGDFEEIASKDAALPFLLN
HPEVTAFFCASDVMALGVLQATRELGLNVPEDLSIIGFDNILLSQYVSPPLTTVGQQPYEMGRKAASMIVNIVEGQDVPH
EAFVNNQLILRESVTKNRR
>Mature_339_residues
MMATLKDVSKASGFSITTVSRALNGYDDVNKETRDKILNVAKELGYSPNILARSLVKKQSKTIGFLVTDLKRESIKDNFM
FETLCGVSDELSDLDYEFVLLSTTTSKQKNKTYSQMCAERQLDGVVIQGLKRDDPYLLEAIESTVPCVLVDIPVEGINTG
YVTSNQLESAKQAVRYLIRLGHRHIAFMNGAPHAYVSTVRYDAYRQVLHDHEIDFQEEYVLNGDFEEIASKDAALPFLLN
HPEVTAFFCASDVMALGVLQATRELGLNVPEDLSIIGFDNILLSQYVSPPLTTVGQQPYEMGRKAASMIVNIVEGQDVPH
EAFVNNQLILRESVTKNRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=35.0299401197605, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1787948, Length=338, Percent_Identity=33.1360946745562, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1790194, Length=314, Percent_Identity=32.484076433121, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789068, Length=346, Percent_Identity=27.1676300578035, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1788474, Length=339, Percent_Identity=25.9587020648968, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787580, Length=333, Percent_Identity=22.5225225225225, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=26.8707482993197, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=26.1261261261261, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1790689, Length=326, Percent_Identity=26.0736196319018, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1789456, Length=341, Percent_Identity=26.6862170087977, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=322, Percent_Identity=26.3975155279503, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790715, Length=325, Percent_Identity=24, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787906, Length=337, Percent_Identity=23.7388724035608, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786268, Length=316, Percent_Identity=21.8354430379747, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37881; Mature: 37881

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMATLKDVSKASGFSITTVSRALNGYDDVNKETRDKILNVAKELGYSPNILARSLVKKQS
CCCCHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KTIGFLVTDLKRESIKDNFMFETLCGVSDELSDLDYEFVLLSTTTSKQKNKTYSQMCAER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHH
QLDGVVIQGLKRDDPYLLEAIESTVPCVLVDIPVEGINTGYVTSNQLESAKQAVRYLIRL
CCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
GHRHIAFMNGAPHAYVSTVRYDAYRQVLHDHEIDFQEEYVLNGDFEEIASKDAALPFLLN
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCCCEEEC
HPEVTAFFCASDVMALGVLQATRELGLNVPEDLSIIGFDNILLSQYVSPPLTTVGQQPYE
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCHHHCCCCHHH
MGRKAASMIVNIVEGQDVPHEAFVNNQLILRESVTKNRR
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEHHHHCCCC
>Mature Secondary Structure
MMATLKDVSKASGFSITTVSRALNGYDDVNKETRDKILNVAKELGYSPNILARSLVKKQS
CCCCHHHHHHCCCCCHHHHHHHHCCHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
KTIGFLVTDLKRESIKDNFMFETLCGVSDELSDLDYEFVLLSTTTSKQKNKTYSQMCAER
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHH
QLDGVVIQGLKRDDPYLLEAIESTVPCVLVDIPVEGINTGYVTSNQLESAKQAVRYLIRL
CCCCHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
GHRHIAFMNGAPHAYVSTVRYDAYRQVLHDHEIDFQEEYVLNGDFEEIASKDAALPFLLN
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCCCEEEC
HPEVTAFFCASDVMALGVLQATRELGLNVPEDLSIIGFDNILLSQYVSPPLTTVGQQPYE
CCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCHHHCCCCHHH
MGRKAASMIVNIVEGQDVPHEAFVNNQLILRESVTKNRR
HHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA