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The map label for this gene is phoR [H]

Identifier: 229916990

GI number: 229916990

Start: 1239328

End: 1240473

Strand: Direct

Name: phoR [H]

Synonym: EAT1b_1264

Alternate gene names: 229916990

Gene position: 1239328-1240473 (Clockwise)

Preceding gene: 229916989

Following gene: 229916991

Centisome position: 41.31

GC content: 48.08

Gene sequence:

>1146_bases
ATGCGCCGATTCCATAGGATTAGGCAGAAACGCTCCGCTCGTCAAAAACAGCAACGCCACACGTTCTCCATCGCTTCCAT
CGTGGAAGAAGTACAGGATGCGATGTGTTATATCGATTTCTCAAGATGTCGTCTGTATTCGAATGATTCTCTTCGGCAAT
GGTACCCTTCTATGACAACACAATCTTTTGACACATTGGAAACATTGACTCGTTATTTATATACCACGTTGGAACAGCAT
GGGGCGGATTCCGCACAAGTCATGCACCTCATCCAGGAATTGCTCGAATGTGCGACCGAAACGGATCCACGAATGACTTC
ACGTCGTCTGCGTTCTAATGACAAGACGTTACATTTGATTTTCAACTTCACGCGGACACCACGTGGCGTTTTCATCATGT
GGCGCGACCAAACGGAAGCCGTCCGCTTTGAAGAACGTAAAGACAGTTTCCTTGCCGAACTGTCTCATGATTTCCGCACG
CCATTGACTGCCATCCATGGCTATACGGAGTTACTCATGACACGCCATCAAGGTTCGGAAAAGTCGTATCAGATGCTAAC
ACAAGTCAAACAAGAATCGGAACGGCTGGAGCGGCTCGTCGATAATTTCCTCGATTATCAAAAAGAGCGCTACACGGCTG
ACCAACTGACCGTGACGACGTTCGCACTCCGTCCTCTTTTAGAGGATGTCATGTCGAGCTATCAGACGACCTCCCCGCTT
CATACAGTGACATTTGAAACGAATGATGTCTCGATTCAAGCAGATGAAAAAAAGTTACGGCAATTGCTCCATAACCTCAT
TGGTAACGCCATCAAGTACTCTCCTGACGGCGGTACGGTCCATACGAGAGTATTTGAATGTGCTGGCACAATCATCTTAT
CTGTATCCGATACCGGCATCGGGATTCCGGAAGAAGCACTTCCATACGTATTTGATGAATATTACCGTGTCGAATCCGCT
GCCCACCAATCGATTAAAGGAACCGGTCTCGGTCTACGAATCTGTAAACGAATCGCCGAAGCCCACGGTTGGGGAATTCA
TGCGGACTCGGTATACGGAGAAGGCACCACCTTTACAATCGAGCTTCATACCAGACAGAGCTGTCCATTGCTGCCATCCC
CTAAACATTCGGTTTCGTTCGACTGA

Upstream 100 bases:

>100_bases
ATACGTTTCTGCTAGGACTATTGCCTGTATAGGGATTCCTTCTCGAATGTCGTATACTTATTGTGAATGCATGTACAAAT
GTTCGCGAGGAGGTAGGTCC

Downstream 100 bases:

>100_bases
TTTGATCGGTCGAACGTTTTTGTCTTACCTCAATTTGAGAATGAAGTGTCTACCTTTTGGGAATAAAGAAATGACGAAGA
GAGGAGGAAATGAAGATGAA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MRRFHRIRQKRSARQKQQRHTFSIASIVEEVQDAMCYIDFSRCRLYSNDSLRQWYPSMTTQSFDTLETLTRYLYTTLEQH
GADSAQVMHLIQELLECATETDPRMTSRRLRSNDKTLHLIFNFTRTPRGVFIMWRDQTEAVRFEERKDSFLAELSHDFRT
PLTAIHGYTELLMTRHQGSEKSYQMLTQVKQESERLERLVDNFLDYQKERYTADQLTVTTFALRPLLEDVMSSYQTTSPL
HTVTFETNDVSIQADEKKLRQLLHNLIGNAIKYSPDGGTVHTRVFECAGTIILSVSDTGIGIPEEALPYVFDEYYRVESA
AHQSIKGTGLGLRICKRIAEAHGWGIHADSVYGEGTTFTIELHTRQSCPLLPSPKHSVSFD

Sequences:

>Translated_381_residues
MRRFHRIRQKRSARQKQQRHTFSIASIVEEVQDAMCYIDFSRCRLYSNDSLRQWYPSMTTQSFDTLETLTRYLYTTLEQH
GADSAQVMHLIQELLECATETDPRMTSRRLRSNDKTLHLIFNFTRTPRGVFIMWRDQTEAVRFEERKDSFLAELSHDFRT
PLTAIHGYTELLMTRHQGSEKSYQMLTQVKQESERLERLVDNFLDYQKERYTADQLTVTTFALRPLLEDVMSSYQTTSPL
HTVTFETNDVSIQADEKKLRQLLHNLIGNAIKYSPDGGTVHTRVFECAGTIILSVSDTGIGIPEEALPYVFDEYYRVESA
AHQSIKGTGLGLRICKRIAEAHGWGIHADSVYGEGTTFTIELHTRQSCPLLPSPKHSVSFD
>Mature_381_residues
MRRFHRIRQKRSARQKQQRHTFSIASIVEEVQDAMCYIDFSRCRLYSNDSLRQWYPSMTTQSFDTLETLTRYLYTTLEQH
GADSAQVMHLIQELLECATETDPRMTSRRLRSNDKTLHLIFNFTRTPRGVFIMWRDQTEAVRFEERKDSFLAELSHDFRT
PLTAIHGYTELLMTRHQGSEKSYQMLTQVKQESERLERLVDNFLDYQKERYTADQLTVTTFALRPLLEDVMSSYQTTSPL
HTVTFETNDVSIQADEKKLRQLLHNLIGNAIKYSPDGGTVHTRVFECAGTIILSVSDTGIGIPEEALPYVFDEYYRVESA
AHQSIKGTGLGLRICKRIAEAHGWGIHADSVYGEGTTFTIELHTRQSCPLLPSPKHSVSFD

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=266, Percent_Identity=28.5714285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=29.3333333333333, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI48994928, Length=250, Percent_Identity=29.6, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=213, Percent_Identity=30.9859154929577, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081816, Length=245, Percent_Identity=29.3877551020408, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788393, Length=222, Percent_Identity=28.8288288288288, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789149, Length=230, Percent_Identity=30, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788549, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1788713, Length=269, Percent_Identity=24.907063197026, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786912, Length=240, Percent_Identity=29.1666666666667, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082128, Length=244, Percent_Identity=24.5901639344262, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786783, Length=222, Percent_Identity=27.9279279279279, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788279, Length=318, Percent_Identity=25.4716981132075, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790346, Length=213, Percent_Identity=27.6995305164319, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790551, Length=201, Percent_Identity=28.3582089552239, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790861, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789403, Length=214, Percent_Identity=29.9065420560748, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787894, Length=228, Percent_Identity=25.4385964912281, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1789808, Length=193, Percent_Identity=24.8704663212435, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44080; Mature: 44080

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFHRIRQKRSARQKQQRHTFSIASIVEEVQDAMCYIDFSRCRLYSNDSLRQWYPSMTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHEECCCCCHHHHCCCCCC
QSFDTLETLTRYLYTTLEQHGADSAQVMHLIQELLECATETDPRMTSRRLRSNDKTLHLI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
FNFTRTPRGVFIMWRDQTEAVRFEERKDSFLAELSHDFRTPLTAIHGYTELLMTRHQGSE
EEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
KSYQMLTQVKQESERLERLVDNFLDYQKERYTADQLTVTTFALRPLLEDVMSSYQTTSPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HTVTFETNDVSIQADEKKLRQLLHNLIGNAIKYSPDGGTVHTRVFECAGTIILSVSDTGI
EEEEEECCCEEEECHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHHHHCEEEEEECCCCC
GIPEEALPYVFDEYYRVESAAHQSIKGTGLGLRICKRIAEAHGWGIHADSVYGEGTTFTI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
ELHTRQSCPLLPSPKHSVSFD
EEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRRFHRIRQKRSARQKQQRHTFSIASIVEEVQDAMCYIDFSRCRLYSNDSLRQWYPSMTT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHEECCCCCHHHHCCCCCC
QSFDTLETLTRYLYTTLEQHGADSAQVMHLIQELLECATETDPRMTSRRLRSNDKTLHLI
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEE
FNFTRTPRGVFIMWRDQTEAVRFEERKDSFLAELSHDFRTPLTAIHGYTELLMTRHQGSE
EEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCH
KSYQMLTQVKQESERLERLVDNFLDYQKERYTADQLTVTTFALRPLLEDVMSSYQTTSPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
HTVTFETNDVSIQADEKKLRQLLHNLIGNAIKYSPDGGTVHTRVFECAGTIILSVSDTGI
EEEEEECCCEEEECHHHHHHHHHHHHHCCCEEECCCCCEEHHHHHHHHCEEEEEECCCCC
GIPEEALPYVFDEYYRVESAAHQSIKGTGLGLRICKRIAEAHGWGIHADSVYGEGTTFTI
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
ELHTRQSCPLLPSPKHSVSFD
EEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]