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The map label for this gene is yhcK [H]

Identifier: 229916953

GI number: 229916953

Start: 1204732

End: 1205808

Strand: Reverse

Name: yhcK [H]

Synonym: EAT1b_1227

Alternate gene names: 229916953

Gene position: 1205808-1204732 (Counterclockwise)

Preceding gene: 229916954

Following gene: 229916944

Centisome position: 40.2

GC content: 39.28

Gene sequence:

>1077_bases
ATGTTCATTAGTCTAATCGCCAATTCTTGTATTTCTTTTACCGGGATCTATTTCATTTCTAAAATTGTAGGTTATCATAC
GACACACATATATATAAAAAATGCATTGCTTGGTATTTTATCAGGACTGCTAGGTATTTTTCTGATCACGCAAAGCATTT
TAGTCGAAGATGTAGTAAAGATTGATTTTCGCTTTCTCATCCTTGTTTTACTTGGATTTTATCGATTAATTTCTCCATTG
TTCATCACAGCCACAATCATCTCAGTCTATCGCTTTAGCTATGGTGTGGACTTTCACTCCATTGTGGCATGTATCGCCAT
TTTTATGATTGTTGTCGCAATCATCCTCACATACAAAATCTCCGCATTGGAAAAACAACTTTTTAAATTCGGGATTACAC
TGAATCTTTTGGCATGTCTGATTGCGGGTGTTGCAATCTACACGAGTGCAGGTTTGACTGAATACGCTGCATACGTTGCG
TTATTGGTCACACTTATTAGTTTTCCTATTGGCATACTCATTACCATCATGAATATTGACCTCTATTTAATCAACCATCG
AGTGAAAGAGTATAAAATTTCAGCTGAAACGGACCATTTGACGGGGCTTGAGAATCGTAGAGCATGGGAGATGCAAATGA
AGAAGATCAAAGAACGTTACGACACTTGTAACGTATTGATTTTAGATATCGATCATTTCAAACAGATCAACGATCGATAT
GGTCACGCAAACGGGGATGAGATTTTGAAGCAGTTCTCAGAACTCTTGCATCGTGAGACGAGGGATCATGACATTAAAGC
ACGAATCGGTGGAGAAGAGTTCGGCATTTTGATTCCGATGCTTTCGCAAAGCGAGGTGATTGGAGTGGCGGAACGGATTC
GAAGTAGTATTTCAAAACATGCGTTCAAATTGTTAGATCAACAAGTCATCCATATATCGGTCTCGATTGGCATCGCGAAT
GGAAAGAGCGAACAGCTTCATGAAATGGTTCATCTGGCAGATGACTGTTTATATCAAGCAAAGAATAGCGGTAGAAATCG
GACCCACATCCATGGAGGTGTGGAGTATGTCCAATGA

Upstream 100 bases:

>100_bases
TTAAAATAAATAATAAAAACCTTAAAATGTTAGTAAATGATGCTAATTGGATATAAGAAGAGAATAGTACTGATATGACA
GATACCGAGGGGATCAACGT

Downstream 100 bases:

>100_bases
AAAAATCACGAAAAAGCGCATCGATCACTTTTGATCAGATGCGCTTTTTCGGTAGGTTCAACAGGACGGGGCAATCAGTC
CATATGAGGTTCCCATACAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MFISLIANSCISFTGIYFISKIVGYHTTHIYIKNALLGILSGLLGIFLITQSILVEDVVKIDFRFLILVLLGFYRLISPL
FITATIISVYRFSYGVDFHSIVACIAIFMIVVAIILTYKISALEKQLFKFGITLNLLACLIAGVAIYTSAGLTEYAAYVA
LLVTLISFPIGILITIMNIDLYLINHRVKEYKISAETDHLTGLENRRAWEMQMKKIKERYDTCNVLILDIDHFKQINDRY
GHANGDEILKQFSELLHRETRDHDIKARIGGEEFGILIPMLSQSEVIGVAERIRSSISKHAFKLLDQQVIHISVSIGIAN
GKSEQLHEMVHLADDCLYQAKNSGRNRTHIHGGVEYVQ

Sequences:

>Translated_358_residues
MFISLIANSCISFTGIYFISKIVGYHTTHIYIKNALLGILSGLLGIFLITQSILVEDVVKIDFRFLILVLLGFYRLISPL
FITATIISVYRFSYGVDFHSIVACIAIFMIVVAIILTYKISALEKQLFKFGITLNLLACLIAGVAIYTSAGLTEYAAYVA
LLVTLISFPIGILITIMNIDLYLINHRVKEYKISAETDHLTGLENRRAWEMQMKKIKERYDTCNVLILDIDHFKQINDRY
GHANGDEILKQFSELLHRETRDHDIKARIGGEEFGILIPMLSQSEVIGVAERIRSSISKHAFKLLDQQVIHISVSIGIAN
GKSEQLHEMVHLADDCLYQAKNSGRNRTHIHGGVEYVQ
>Mature_358_residues
MFISLIANSCISFTGIYFISKIVGYHTTHIYIKNALLGILSGLLGIFLITQSILVEDVVKIDFRFLILVLLGFYRLISPL
FITATIISVYRFSYGVDFHSIVACIAIFMIVVAIILTYKISALEKQLFKFGITLNLLACLIAGVAIYTSAGLTEYAAYVA
LLVTLISFPIGILITIMNIDLYLINHRVKEYKISAETDHLTGLENRRAWEMQMKKIKERYDTCNVLILDIDHFKQINDRY
GHANGDEILKQFSELLHRETRDHDIKARIGGEEFGILIPMLSQSEVIGVAERIRSSISKHAFKLLDQQVIHISVSIGIAN
GKSEQLHEMVHLADDCLYQAKNSGRNRTHIHGGVEYVQ

Specific function: Unknown

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=187, Percent_Identity=31.0160427807487, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786584, Length=330, Percent_Identity=25.1515151515152, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1787262, Length=169, Percent_Identity=31.3609467455621, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693134, Length=159, Percent_Identity=38.3647798742138, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=38.8888888888889, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87082007, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788381, Length=209, Percent_Identity=29.6650717703349, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788085, Length=181, Percent_Identity=29.2817679558011, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI1787816, Length=168, Percent_Identity=32.1428571428571, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787541, Length=165, Percent_Identity=33.9393939393939, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788956, Length=159, Percent_Identity=34.5911949685535, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081977, Length=151, Percent_Identity=31.7880794701987, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787056, Length=156, Percent_Identity=29.4871794871795, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR011620 [H]

Pfam domain/function: PF07694 5TM-5TMR_LYT; PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 40413; Mature: 40413

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFISLIANSCISFTGIYFISKIVGYHTTHIYIKNALLGILSGLLGIFLITQSILVEDVVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDFRFLILVLLGFYRLISPLFITATIISVYRFSYGVDFHSIVACIAIFMIVVAIILTYKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SALEKQLFKFGITLNLLACLIAGVAIYTSAGLTEYAAYVALLVTLISFPIGILITIMNID
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLINHRVKEYKISAETDHLTGLENRRAWEMQMKKIKERYDTCNVLILDIDHFKQINDRY
HHHHHHHHHHHEECCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHC
GHANGDEILKQFSELLHRETRDHDIKARIGGEEFGILIPMLSQSEVIGVAERIRSSISKH
CCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
AFKLLDQQVIHISVSIGIANGKSEQLHEMVHLADDCLYQAKNSGRNRTHIHGGVEYVQ
HHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC
>Mature Secondary Structure
MFISLIANSCISFTGIYFISKIVGYHTTHIYIKNALLGILSGLLGIFLITQSILVEDVVK
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDFRFLILVLLGFYRLISPLFITATIISVYRFSYGVDFHSIVACIAIFMIVVAIILTYKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
SALEKQLFKFGITLNLLACLIAGVAIYTSAGLTEYAAYVALLVTLISFPIGILITIMNID
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYLINHRVKEYKISAETDHLTGLENRRAWEMQMKKIKERYDTCNVLILDIDHFKQINDRY
HHHHHHHHHHHEECCCCCHHCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHC
GHANGDEILKQFSELLHRETRDHDIKARIGGEEFGILIPMLSQSEVIGVAERIRSSISKH
CCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHH
AFKLLDQQVIHISVSIGIANGKSEQLHEMVHLADDCLYQAKNSGRNRTHIHGGVEYVQ
HHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]