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The map label for this gene is rfbB [H]

Identifier: 229916911

GI number: 229916911

Start: 1154774

End: 1155826

Strand: Reverse

Name: rfbB [H]

Synonym: EAT1b_1184

Alternate gene names: 229916911

Gene position: 1155826-1154774 (Counterclockwise)

Preceding gene: 229916912

Following gene: 229916910

Centisome position: 38.53

GC content: 51.38

Gene sequence:

>1053_bases
ATGCATACGATCCTAGTGACTGGCGGAGCCGGGTTCATCGGTTCAAATTTCATAAAACACTTTCGGGACAAACATCCAAC
GTCACGCATCCTTAACGTCGACGCGTTGACCTATGCCGGCAATTTAGAGAACGTCCAAGCGTTCGAGCACGATCCGCTCT
ACGTGTTTCGGAAAGTAGACATCCGCGACCGTCCGGCGATTGATGAACTGTTCTCTCTCTTTGAGATTGACGAAGTCGTG
CATTTTGCGGCGGAATCGCATGTCGACCGAAGTATCCTTGAGCCGGAGTTGTTCCTCTCGACGAATATCCTGGGCACACA
AGTGTTGCTCGACGCGGCCATGCGGCATTGGAAACGGTATTCGGACGATAAATACAGTCGAGAATACCGTGATGACGTCT
GTTTCGTCCATGTCTCGACGGATGAGGTATACGGGTCGCTCGGGGAGACGGGGTTCTTTACAGAAAAGACACCACTCGCC
CCGAACAGTCCTTATTCCGCCTCGAAGGCCGGGTCTGATTTGCTCGTTCGTTCCTATCATGAGACGTACGGCTTACCGAT
TAAGATCACCCGTTGCAGCAACAATTACGGTCCGTATCAATTCCCCGAGAAACTGATTCCGCTCATGGTGCACCATTGTA
TGACCGATCAACTGCTCCCGGTGTATGGGGACGGAGGACATATCCGGGATTGGTTACATGTGCAGGACCACTGTGAAGCC
ATCAATCTCGTTCGACAAAAGGGGCAGGTCGGAGAAGTCTATAACATCGGGGGACATAACGAGAAGACGAACCTGGAGCT
CGTCCGAACGATTTTACGAGCGTTTGACTTACCTGATGACCGCATCCAATTCGTGACGGATCGCCCTGGCCATGACCGCC
GTTATGCCATTGACGCGAAGAAAATGCAAGACGAACTCGGGTGGGTGCCACGCTACAGTTTTGAACAAGGAATCGAAGAG
ACAATCGAATGGTACCGGTCACATCCGGAATGGGTCGAGCGCATCGTTTCGGGAGCGTATAAAACTTATTATCAAGACAT
CTATTCTTCCTAA

Upstream 100 bases:

>100_bases
TTCATCACGCGAGAGCAATTGGCCGACATCGGTCAGACGCTCAAGATGACCGATTACGGACAGTATTTGATATCGCTTCT
AGAGGAAGGAGAACAGACCA

Downstream 100 bases:

>100_bases
GTCTGAGTCGTTCTATCATCCCGAGAAAGGAGGGGATCGACATGGTGATGGTCAGCATCGACTGCATCACGTATAACCAC
GAACCGTACATCGCCAAAAC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MHTILVTGGAGFIGSNFIKHFRDKHPTSRILNVDALTYAGNLENVQAFEHDPLYVFRKVDIRDRPAIDELFSLFEIDEVV
HFAAESHVDRSILEPELFLSTNILGTQVLLDAAMRHWKRYSDDKYSREYRDDVCFVHVSTDEVYGSLGETGFFTEKTPLA
PNSPYSASKAGSDLLVRSYHETYGLPIKITRCSNNYGPYQFPEKLIPLMVHHCMTDQLLPVYGDGGHIRDWLHVQDHCEA
INLVRQKGQVGEVYNIGGHNEKTNLELVRTILRAFDLPDDRIQFVTDRPGHDRRYAIDAKKMQDELGWVPRYSFEQGIEE
TIEWYRSHPEWVERIVSGAYKTYYQDIYSS

Sequences:

>Translated_350_residues
MHTILVTGGAGFIGSNFIKHFRDKHPTSRILNVDALTYAGNLENVQAFEHDPLYVFRKVDIRDRPAIDELFSLFEIDEVV
HFAAESHVDRSILEPELFLSTNILGTQVLLDAAMRHWKRYSDDKYSREYRDDVCFVHVSTDEVYGSLGETGFFTEKTPLA
PNSPYSASKAGSDLLVRSYHETYGLPIKITRCSNNYGPYQFPEKLIPLMVHHCMTDQLLPVYGDGGHIRDWLHVQDHCEA
INLVRQKGQVGEVYNIGGHNEKTNLELVRTILRAFDLPDDRIQFVTDRPGHDRRYAIDAKKMQDELGWVPRYSFEQGIEE
TIEWYRSHPEWVERIVSGAYKTYYQDIYSS
>Mature_350_residues
MHTILVTGGAGFIGSNFIKHFRDKHPTSRILNVDALTYAGNLENVQAFEHDPLYVFRKVDIRDRPAIDELFSLFEIDEVV
HFAAESHVDRSILEPELFLSTNILGTQVLLDAAMRHWKRYSDDKYSREYRDDVCFVHVSTDEVYGSLGETGFFTEKTPLA
PNSPYSASKAGSDLLVRSYHETYGLPIKITRCSNNYGPYQFPEKLIPLMVHHCMTDQLLPVYGDGGHIRDWLHVQDHCEA
INLVRQKGQVGEVYNIGGHNEKTNLELVRTILRAFDLPDDRIQFVTDRPGHDRRYAIDAKKMQDELGWVPRYSFEQGIEE
TIEWYRSHPEWVERIVSGAYKTYYQDIYSS

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=37.6506024096386, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI42516563, Length=342, Percent_Identity=24.8538011695906, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI56237023, Length=350, Percent_Identity=24, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=350, Percent_Identity=24, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=350, Percent_Identity=24, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1788353, Length=361, Percent_Identity=50.415512465374, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=50.7082152974504, Blast_Score=349, Evalue=1e-97,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=24.8571428571429, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17568069, Length=331, Percent_Identity=35.9516616314199, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=330, Percent_Identity=34.2424242424242, Blast_Score=188, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI71982035, Length=365, Percent_Identity=24.6575342465753, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=334, Percent_Identity=22.7544910179641, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=191, Percent_Identity=30.3664921465969, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=191, Percent_Identity=30.3664921465969, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=191, Percent_Identity=30.3664921465969, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=191, Percent_Identity=30.3664921465969, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=186, Percent_Identity=30.6451612903226, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=192, Percent_Identity=29.1666666666667, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=362, Percent_Identity=27.0718232044199, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=26.9230769230769, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=23.7388724035608, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40441; Mature: 40441

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTILVTGGAGFIGSNFIKHFRDKHPTSRILNVDALTYAGNLENVQAFEHDPLYVFRKVD
CCEEEEECCCCHHHHHHHHHHHHCCCHHHEEECCCEEECCCCCCCHHCCCCCEEEEEEEC
IRDRPAIDELFSLFEIDEVVHFAAESHVDRSILEPELFLSTNILGTQVLLDAAMRHWKRY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHC
SDDKYSREYRDDVCFVHVSTDEVYGSLGETGFFTEKTPLAPNSPYSASKAGSDLLVRSYH
CCCHHHHHHCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ETYGLPIKITRCSNNYGPYQFPEKLIPLMVHHCMTDQLLPVYGDGGHIRDWLHVQDHCEA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHH
INLVRQKGQVGEVYNIGGHNEKTNLELVRTILRAFDLPDDRIQFVTDRPGHDRRYAIDAK
HHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECHH
KMQDELGWVPRYSFEQGIEETIEWYRSHPEWVERIVSGAYKTYYQDIYSS
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTILVTGGAGFIGSNFIKHFRDKHPTSRILNVDALTYAGNLENVQAFEHDPLYVFRKVD
CCEEEEECCCCHHHHHHHHHHHHCCCHHHEEECCCEEECCCCCCCHHCCCCCEEEEEEEC
IRDRPAIDELFSLFEIDEVVHFAAESHVDRSILEPELFLSTNILGTQVLLDAAMRHWKRY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHC
SDDKYSREYRDDVCFVHVSTDEVYGSLGETGFFTEKTPLAPNSPYSASKAGSDLLVRSYH
CCCHHHHHHCCCEEEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ETYGLPIKITRCSNNYGPYQFPEKLIPLMVHHCMTDQLLPVYGDGGHIRDWLHVQDHCEA
HHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHH
INLVRQKGQVGEVYNIGGHNEKTNLELVRTILRAFDLPDDRIQFVTDRPGHDRRYAIDAK
HHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECHH
KMQDELGWVPRYSFEQGIEETIEWYRSHPEWVERIVSGAYKTYYQDIYSS
HHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]