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The map label for this gene is ywbA [H]

Identifier: 229916868

GI number: 229916868

Start: 1111582

End: 1112901

Strand: Direct

Name: ywbA [H]

Synonym: EAT1b_1141

Alternate gene names: 229916868

Gene position: 1111582-1112901 (Clockwise)

Preceding gene: 229916867

Following gene: 229916869

Centisome position: 37.05

GC content: 52.95

Gene sequence:

>1320_bases
ATGAACAAGTTTATTGAAATCGCGGGACGCATTGGGTCCCAAAGGCACTTAGTCGCCATCCGTGACGGATTCGTTTCCGT
CATGCCACTCATCATCGTCGGGTCGCTCGCGGTCCTCATCAACAACTTCCCAGCCATCAACATCGGTGGTCTCTCACTCG
ACTTCGTCGGATCGATGAACAGTATGTTCGGCGAAGGAAACTGGCAAGGGCTCGGCGGTAGCATTTGGAACGCGTCCTTC
GCGATACTCGGGCTCCTGATCGCCTTCTCCATCGGGTATAACTTGGCCCGTTCATACGGGATTGACGGGCTCGCGGCGGG
GATCTTGTCGCTCGCCTCGTATATCATGCTCGTACCGGTCACTGCTGACTGGGGTCTCAACTTCGCATGGCTCGGCGCAC
AAGGTCTCTTCGTCGCCATCATCGTATCGATTGTCGTTACAGAAATGTTCCGCTACCTCATCAATACGAAACTGACGATC
AAGATGCCAGAAGGTGTACCAGGTGGGGTCACTCGTTCATTCCAAGCGCTCATCCCATCGATGATCATCTTGACGGTCGT
CGCAGGATTCCAGCTCTTCATGACGCTCGTCGTCGAACTCAGTGTATTTGAAGTCATCTTTAAAGCGATCCAACAACCGC
TCCAAGGATTCGGCGACTCACTCCCGGCTGCCTTGCTCATCGCCTTCTTGAACCACATCCTTTGGTTCTTCGGGCTTCAC
GGGACGAACATCATCGGCTCGGTCATCGAACCGGTCTATCTCCCGCTCATCGAGCAAAACTTGGCTGCCTTCCAAGCTGG
AACGTCAGCGTTTGATGTACCATACACGGTCACAAAACCGTTCTTGGATGCGTTCGTCTTCATGGGTGGATCGGGTACGA
CGTTCGCGCTCTTACTCGCGATCTTCATCGTCGTCCGCAAACAACGGAATCACCCGTATCACCAAGTGGCAAAACTGTCG
ACACCAGCCAGTCTCTTTAACATCAACGAACCGGTCATCTTCGGTCTTCCAATTGTATTGAACCCGATCATGCTCGTACC
GTTCATCTTAGTGCCGGTCACGCTCACCATCGTCTCTTATGTCGCGCTCTCGACAGGCATGGTTCCGAAGACAGTCGCCA
TCGTCCCTTGGACAACGCCACCAATCATCAGTGGCTATTTGGTAACGGGCGGTAGCGTTCGCGGCGTCTTGCTCCAAATC
GTCAACTTGTCGATTGCGACAGCGATGTATATTCCATTCGTCATGGCAGGAGCGAAGGCGATGACAGCCGAAATCAATAA
AAATAAAGCTGCTACTGAGGAAGGAGAAAAGATTTCGTGA

Upstream 100 bases:

>100_bases
ACCCGCTCATTCAAGACGAGAAACGGATCGACCCGCTTCTTAAAGAGCTGTTCACAGCGCACGAGGCGTACCTACCCCAA
TTCAAGAAGGAGGTTTACTC

Downstream 100 bases:

>100_bases
AAACCGAAACCATCCAAGAACTCTCGTTTATGATTATCTTACATGCCGGAAATGCACGCTCATCTGCATTCGAGGCTATC
GTTGCCGCGAAACGCGGTGA

Product: PTS system, cellobiose-specific IIC subunit

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MNKFIEIAGRIGSQRHLVAIRDGFVSVMPLIIVGSLAVLINNFPAINIGGLSLDFVGSMNSMFGEGNWQGLGGSIWNASF
AILGLLIAFSIGYNLARSYGIDGLAAGILSLASYIMLVPVTADWGLNFAWLGAQGLFVAIIVSIVVTEMFRYLINTKLTI
KMPEGVPGGVTRSFQALIPSMIILTVVAGFQLFMTLVVELSVFEVIFKAIQQPLQGFGDSLPAALLIAFLNHILWFFGLH
GTNIIGSVIEPVYLPLIEQNLAAFQAGTSAFDVPYTVTKPFLDAFVFMGGSGTTFALLLAIFIVVRKQRNHPYHQVAKLS
TPASLFNINEPVIFGLPIVLNPIMLVPFILVPVTLTIVSYVALSTGMVPKTVAIVPWTTPPIISGYLVTGGSVRGVLLQI
VNLSIATAMYIPFVMAGAKAMTAEINKNKAATEEGEKIS

Sequences:

>Translated_439_residues
MNKFIEIAGRIGSQRHLVAIRDGFVSVMPLIIVGSLAVLINNFPAINIGGLSLDFVGSMNSMFGEGNWQGLGGSIWNASF
AILGLLIAFSIGYNLARSYGIDGLAAGILSLASYIMLVPVTADWGLNFAWLGAQGLFVAIIVSIVVTEMFRYLINTKLTI
KMPEGVPGGVTRSFQALIPSMIILTVVAGFQLFMTLVVELSVFEVIFKAIQQPLQGFGDSLPAALLIAFLNHILWFFGLH
GTNIIGSVIEPVYLPLIEQNLAAFQAGTSAFDVPYTVTKPFLDAFVFMGGSGTTFALLLAIFIVVRKQRNHPYHQVAKLS
TPASLFNINEPVIFGLPIVLNPIMLVPFILVPVTLTIVSYVALSTGMVPKTVAIVPWTTPPIISGYLVTGGSVRGVLLQI
VNLSIATAMYIPFVMAGAKAMTAEINKNKAATEEGEKIS
>Mature_439_residues
MNKFIEIAGRIGSQRHLVAIRDGFVSVMPLIIVGSLAVLINNFPAINIGGLSLDFVGSMNSMFGEGNWQGLGGSIWNASF
AILGLLIAFSIGYNLARSYGIDGLAAGILSLASYIMLVPVTADWGLNFAWLGAQGLFVAIIVSIVVTEMFRYLINTKLTI
KMPEGVPGGVTRSFQALIPSMIILTVVAGFQLFMTLVVELSVFEVIFKAIQQPLQGFGDSLPAALLIAFLNHILWFFGLH
GTNIIGSVIEPVYLPLIEQNLAAFQAGTSAFDVPYTVTKPFLDAFVFMGGSGTTFALLLAIFIVVRKQRNHPYHQVAKLS
TPASLFNINEPVIFGLPIVLNPIMLVPFILVPVTLTIVSYVALSTGMVPKTVAIVPWTTPPIISGYLVTGGSVRGVLLQI
VNLSIATAMYIPFVMAGAKAMTAEINKNKAATEEGEKIS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=441, Percent_Identity=36.734693877551, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 47056; Mature: 47056

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFIEIAGRIGSQRHLVAIRDGFVSVMPLIIVGSLAVLINNFPAINIGGLSLDFVGSMN
CCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEHHHCHH
SMFGEGNWQGLGGSIWNASFAILGLLIAFSIGYNLARSYGIDGLAAGILSLASYIMLVPV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEE
TADWGLNFAWLGAQGLFVAIIVSIVVTEMFRYLINTKLTIKMPEGVPGGVTRSFQALIPS
ECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
MIILTVVAGFQLFMTLVVELSVFEVIFKAIQQPLQGFGDSLPAALLIAFLNHILWFFGLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
GTNIIGSVIEPVYLPLIEQNLAAFQAGTSAFDVPYTVTKPFLDAFVFMGGSGTTFALLLA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
IFIVVRKQRNHPYHQVAKLSTPASLFNINEPVIFGLPIVLNPIMLVPFILVPVTLTIVSY
HHHHHHHHCCCCHHHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
VALSTGMVPKTVAIVPWTTPPIISGYLVTGGSVRGVLLQIVNLSIATAMYIPFVMAGAKA
HHHHCCCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MTAEINKNKAATEEGEKIS
HHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKFIEIAGRIGSQRHLVAIRDGFVSVMPLIIVGSLAVLINNFPAINIGGLSLDFVGSMN
CCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEHHHCHH
SMFGEGNWQGLGGSIWNASFAILGLLIAFSIGYNLARSYGIDGLAAGILSLASYIMLVPV
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEE
TADWGLNFAWLGAQGLFVAIIVSIVVTEMFRYLINTKLTIKMPEGVPGGVTRSFQALIPS
ECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHH
MIILTVVAGFQLFMTLVVELSVFEVIFKAIQQPLQGFGDSLPAALLIAFLNHILWFFGLH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
GTNIIGSVIEPVYLPLIEQNLAAFQAGTSAFDVPYTVTKPFLDAFVFMGGSGTTFALLLA
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
IFIVVRKQRNHPYHQVAKLSTPASLFNINEPVIFGLPIVLNPIMLVPFILVPVTLTIVSY
HHHHHHHHCCCCHHHHHHCCCCHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
VALSTGMVPKTVAIVPWTTPPIISGYLVTGGSVRGVLLQIVNLSIATAMYIPFVMAGAKA
HHHHCCCCCCEEEEECCCCCCHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHH
MTAEINKNKAATEEGEKIS
HHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]