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The map label for this gene is eno [H]

Identifier: 229916588

GI number: 229916588

Start: 812989

End: 814281

Strand: Reverse

Name: eno [H]

Synonym: EAT1b_0860

Alternate gene names: 229916588

Gene position: 814281-812989 (Counterclockwise)

Preceding gene: 229916589

Following gene: 229916587

Centisome position: 27.14

GC content: 48.18

Gene sequence:

>1293_bases
ATGTCAATGATTACAGAAATTTACGCACGCGAGATTTTAGATTCACGCGGTAACCCATCAGTAGAAGTAGAAGTCTTCAC
AGAAGACGGCGGTTTCGGCCGTGCCCTCGTCCCATCAGGCGCATCAACAGGTGAGCACGAAGCAGTTGAGCTTCGCGATG
GCGACAAGTCACGTTACCTCGGTAAAGGTGTCTTGAAAGCAGTTGCGAACGTGAACGACACGATTGCACCAGAACTCATC
GGCTACGATGTATTCGACCAAAACGCAATCGATGCGAAAATGATCGAACTCGACGGTACGCCAAACAAAGCAAAACTTGG
TGCAAACGCAATCCTCGGTGTATCGATGGCAGCAGCTCACGCAGCAGCAGATGAGCTTGGTCTTCCACTTTACACTTACC
TCGGTGGATTCAACGCGAAGACACTTCCAACACCAATGATGAACATCATCAACGGTGGTTCGCACGCGGACAACAACGTG
GACTTCCAAGAGTTCATGATCATGCCTGTTGGCGCGCCTACATTCCGTGAAGCGCTCCGTATGGGTGCTGAAGTATTCCA
CGCCCTCAAATCAGTTCTTTCTGGTATGGGTCTTAACACAGCTGTCGGTGACGAAGGTGGTTTCGCACCAAACTTGAAGT
CTAACGAAGAAGCGATCACAGTTATCCTTGAAGCGATCGAAAAAGCTGGTTACAAACCAGGTGAAGACGTCTACCTCGCA
ATGGACGTTGCATCTTCTGAGTTCTACGACAAATCAACTGGTAAATACGAACTCGCTGGCGAAGGCAAGTCACTCACAAC
TGCTGAGCTTGTAGACTTCTACGCTGAGCTCGTTGACAAGTACCCAATCCTCTCAATCGAAGATGGTTGTGACGAAAACG
ACTGGGATGGCCACAAGCTTCTCACAGACAAAATCGGACACAAAGTTCAACTCGTTGGGGATGACCTCTTCGTAACGAAC
ACATCTAAACTTGCTGAAGGTATCGAAAAAGGAATCGCAAACTCGATCCTCATCAAAGTTAACCAAATCGGTACGCTCAC
AGAAACATTCGACGCGATCGAAATGGCTAAAAAAGCTGGTTACACAGCAGTCGTCTCACACCGTTCTGGTGAAACAGAAG
ACGCGACAATCGCAGACATCGCTGTTGCGACAAACGCTGGACAAATCAAAACTGGTTCACTTTCACGTACAGACCGTATC
GCGAAATACAACCAACTTCTCCGCATCGAAGACATGCTCGGCGATGTAGCGAAATACGACGGTATCAAGTCGTTCTACAA
CCTCAAAAAATAA

Upstream 100 bases:

>100_bases
TCTTCACCTTCTCGGTGGAGAGCAACCAGCTGAAATGACTGGTTCGTCAATCGTTACAAAATAATACAACTATTTTTCAA
ACGAAAAGGAGCGATTTTTC

Downstream 100 bases:

>100_bases
TTGATTGTTTCAGGCTCGCCAATCGGCGGGCCTGTTTTTTTGTGTGTGGAATATCCGTTTAATCAACGGTGGAAAAGCTG
ATTGGTCGTCTATCCGTCTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MSMITEIYAREILDSRGNPSVEVEVFTEDGGFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVANVNDTIAPELI
GYDVFDQNAIDAKMIELDGTPNKAKLGANAILGVSMAAAHAAADELGLPLYTYLGGFNAKTLPTPMMNIINGGSHADNNV
DFQEFMIMPVGAPTFREALRMGAEVFHALKSVLSGMGLNTAVGDEGGFAPNLKSNEEAITVILEAIEKAGYKPGEDVYLA
MDVASSEFYDKSTGKYELAGEGKSLTTAELVDFYAELVDKYPILSIEDGCDENDWDGHKLLTDKIGHKVQLVGDDLFVTN
TSKLAEGIEKGIANSILIKVNQIGTLTETFDAIEMAKKAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSLSRTDRI
AKYNQLLRIEDMLGDVAKYDGIKSFYNLKK

Sequences:

>Translated_430_residues
MSMITEIYAREILDSRGNPSVEVEVFTEDGGFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVANVNDTIAPELI
GYDVFDQNAIDAKMIELDGTPNKAKLGANAILGVSMAAAHAAADELGLPLYTYLGGFNAKTLPTPMMNIINGGSHADNNV
DFQEFMIMPVGAPTFREALRMGAEVFHALKSVLSGMGLNTAVGDEGGFAPNLKSNEEAITVILEAIEKAGYKPGEDVYLA
MDVASSEFYDKSTGKYELAGEGKSLTTAELVDFYAELVDKYPILSIEDGCDENDWDGHKLLTDKIGHKVQLVGDDLFVTN
TSKLAEGIEKGIANSILIKVNQIGTLTETFDAIEMAKKAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSLSRTDRI
AKYNQLLRIEDMLGDVAKYDGIKSFYNLKK
>Mature_429_residues
SMITEIYAREILDSRGNPSVEVEVFTEDGGFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVLKAVANVNDTIAPELIG
YDVFDQNAIDAKMIELDGTPNKAKLGANAILGVSMAAAHAAADELGLPLYTYLGGFNAKTLPTPMMNIINGGSHADNNVD
FQEFMIMPVGAPTFREALRMGAEVFHALKSVLSGMGLNTAVGDEGGFAPNLKSNEEAITVILEAIEKAGYKPGEDVYLAM
DVASSEFYDKSTGKYELAGEGKSLTTAELVDFYAELVDKYPILSIEDGCDENDWDGHKLLTDKIGHKVQLVGDDLFVTNT
SKLAEGIEKGIANSILIKVNQIGTLTETFDAIEMAKKAGYTAVVSHRSGETEDATIADIAVATNAGQIKTGSLSRTDRIA
KYNQLLRIEDMLGDVAKYDGIKSFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=53.2110091743119, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=438, Percent_Identity=52.2831050228311, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=438, Percent_Identity=52.2831050228311, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.8348623853211, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=436, Percent_Identity=47.2477064220183, Blast_Score=363, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=339, Percent_Identity=25.3687315634218, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI169201757, Length=339, Percent_Identity=25.3687315634218, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI239744207, Length=339, Percent_Identity=25.3687315634218, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=66.1214953271028, Blast_Score=540, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.3642691415313, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.3642691415313, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=133, Percent_Identity=66.1654135338346, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=51.9630484988453, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.4226327944573, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.4226327944573, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.4226327944573, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=51.5011547344111, Blast_Score=376, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=435, Percent_Identity=53.3333333333333, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=435, Percent_Identity=53.3333333333333, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=435, Percent_Identity=53.3333333333333, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=435, Percent_Identity=53.3333333333333, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=435, Percent_Identity=53.3333333333333, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=435, Percent_Identity=53.3333333333333, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46103; Mature: 45971

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMITEIYAREILDSRGNPSVEVEVFTEDGGFGRALVPSGASTGEHEAVELRDGDKSRYL
CCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVANVNDTIAPELIGYDVFDQNAIDAKMIELDGTPNKAKLGANAILGVSMAAAH
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAADELGLPLYTYLGGFNAKTLPTPMMNIINGGSHADNNVDFQEFMIMPVGAPTFREALR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKSVLSGMGLNTAVGDEGGFAPNLKSNEEAITVILEAIEKAGYKPGEDVYLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVASSEFYDKSTGKYELAGEGKSLTTAELVDFYAELVDKYPILSIEDGCDENDWDGHKL
EEECCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCH
LTDKIGHKVQLVGDDLFVTNTSKLAEGIEKGIANSILIKVNQIGTLTETFDAIEMAKKAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDATIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDMLGDVAKYD
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIKSFYNLKK
CHHHHHCCCC
>Mature Secondary Structure 
SMITEIYAREILDSRGNPSVEVEVFTEDGGFGRALVPSGASTGEHEAVELRDGDKSRYL
CHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVANVNDTIAPELIGYDVFDQNAIDAKMIELDGTPNKAKLGANAILGVSMAAAH
HHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCEEEEEECCCCCHHHCCCCHHHHHHHHHHH
AAADELGLPLYTYLGGFNAKTLPTPMMNIINGGSHADNNVDFQEFMIMPVGAPTFREALR
HHHHHCCCCHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHH
MGAEVFHALKSVLSGMGLNTAVGDEGGFAPNLKSNEEAITVILEAIEKAGYKPGEDVYLA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVASSEFYDKSTGKYELAGEGKSLTTAELVDFYAELVDKYPILSIEDGCDENDWDGHKL
EEECCCHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCH
LTDKIGHKVQLVGDDLFVTNTSKLAEGIEKGIANSILIKVNQIGTLTETFDAIEMAKKAG
HHHHCCCEEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
YTAVVSHRSGETEDATIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDMLGDVAKYD
CEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GIKSFYNLKK
CHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA