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The map label for this gene is braB [H]

Identifier: 229916523

GI number: 229916523

Start: 747472

End: 748791

Strand: Reverse

Name: braB [H]

Synonym: EAT1b_0793

Alternate gene names: 229916523

Gene position: 748791-747472 (Counterclockwise)

Preceding gene: 229916524

Following gene: 229916522

Centisome position: 24.96

GC content: 52.05

Gene sequence:

>1320_bases
ATGAAAAAATCGACATTGTTCCCGTTAGCGTTTACGATTTTTGCTTTATTCTTTGGGGCCGGGAACTTGCTGTTCCCACC
AGAGCTCGGCGCCTTGGCGGGGGAAAACCTCGTACCGGCGATGATCGGGTTCGTCATTACGGGTGTCGGTTTACCGCTTC
TTGCTTTGTTCGCAGTCGCTCGGGTCGGCGGTGGGACAGACCAAATTGCTCGTTATATTCCAAAGCGTCTCGCACTCGTT
TTGACAGGATTGATGTATGTAGCCATCGGACCATTCTTCGGAATTCCGCGTACGGCTGCCGTCACGTATGAAATGGGACT
CGTCCCGTTTCTCGATGCGCCTTCTACTTTGACACTCGCGATGAGTTCGACCGTCTTTTTCGGGTTGACGTTCTTCTTGA
CGCTTCGTGCTCAAAAGTTGATTGAAGTCATCGGACGCATCATCACACCAATCCTCTTGTTCTTACTCGCAGCGATTGGA
ATCACAAACCTCATCTCGCCTCTCGGAGAGCCGGGAGCGCCAGCAGAAGGGTTTGAGACATGGGTGGGCGCACTCGGCCA
AGGTTTCCAACAAGGCTATTTGACAATGGATGTATTCGGGGCCCTCGTCTTTGGGGCAATCGTCCTCGTCACGTTGAAAG
CCAATGGATTGAGTGACCAAAAAGAAGCGTCTTCGCTCCTTCGCTCTGCAGGGATGCTTGCCGTTACCTGTTTGATGCTC
GTCTATATCTCGCTCGGTTCGATCGGTGCGAATATGACAGTCGCAGAAGGAACGACAAGTGGCGCAAGCCTTCTCCAAGC
GTTCGCAGGTACGTCTTACGGACAAGTCGGAATGCTCGCACTCGGGTTAGCGGTCTTCCTCGCTTGTTTGACGACAGCAG
TTGGACTCATCTCAGAGTTCTCACGCTTCATCAGCAACCAGTTTGATTCTCTCAAATATCATCATGTCGTCATCGCGACA
ACATTGTTTGGGTTCATGATCTCACTCGTCGGTTTGAGCTCGTTGATTCAAATCGCCGTGCCGCTTCTCACGCTCCTCTA
TCCGGTCATGATCGCACTCGTGCTCGTATCGGTGACGGAACGTCTTCAACGAAACCCTGGTGTGGCGATGAAAGCGACAA
TTTACACGGCGCTCGTGCTCTCGTTCTTTGAAACGTGGAATGGCATCCAGCCTTCTGCGGTCACTTCTTGGATCGGAAGC
TTACCAATGGCGGATATCGGACTTGCCTGGGCAGTTCCGACGATGATCGTCTATGCGATCACGCTTCTTCTTCCGAAGGG
AACGTCACAAGAATCACTCGCTCAATCACGCATCTCATAA

Upstream 100 bases:

>100_bases
GTGTGTATAATGAAATAGACCAAGCGATATATTGTCAGAAAATTTAGTTATTATTTTTTTGTTAAGTCGTTCGGAATATC
AAGTGTATGGAGGTAGTTGT

Downstream 100 bases:

>100_bases
GTCTCGCCCATCCTTTGGTCAAGGATGGGTTTTTTCTGTCTTATTGTTTGCCTGGATGTGAACTCAAACAGTAAAATGGA
AAGATGAAAAGGGAGTGACA

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-II [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MKKSTLFPLAFTIFALFFGAGNLLFPPELGALAGENLVPAMIGFVITGVGLPLLALFAVARVGGGTDQIARYIPKRLALV
LTGLMYVAIGPFFGIPRTAAVTYEMGLVPFLDAPSTLTLAMSSTVFFGLTFFLTLRAQKLIEVIGRIITPILLFLLAAIG
ITNLISPLGEPGAPAEGFETWVGALGQGFQQGYLTMDVFGALVFGAIVLVTLKANGLSDQKEASSLLRSAGMLAVTCLML
VYISLGSIGANMTVAEGTTSGASLLQAFAGTSYGQVGMLALGLAVFLACLTTAVGLISEFSRFISNQFDSLKYHHVVIAT
TLFGFMISLVGLSSLIQIAVPLLTLLYPVMIALVLVSVTERLQRNPGVAMKATIYTALVLSFFETWNGIQPSAVTSWIGS
LPMADIGLAWAVPTMIVYAITLLLPKGTSQESLAQSRIS

Sequences:

>Translated_439_residues
MKKSTLFPLAFTIFALFFGAGNLLFPPELGALAGENLVPAMIGFVITGVGLPLLALFAVARVGGGTDQIARYIPKRLALV
LTGLMYVAIGPFFGIPRTAAVTYEMGLVPFLDAPSTLTLAMSSTVFFGLTFFLTLRAQKLIEVIGRIITPILLFLLAAIG
ITNLISPLGEPGAPAEGFETWVGALGQGFQQGYLTMDVFGALVFGAIVLVTLKANGLSDQKEASSLLRSAGMLAVTCLML
VYISLGSIGANMTVAEGTTSGASLLQAFAGTSYGQVGMLALGLAVFLACLTTAVGLISEFSRFISNQFDSLKYHHVVIAT
TLFGFMISLVGLSSLIQIAVPLLTLLYPVMIALVLVSVTERLQRNPGVAMKATIYTALVLSFFETWNGIQPSAVTSWIGS
LPMADIGLAWAVPTMIVYAITLLLPKGTSQESLAQSRIS
>Mature_439_residues
MKKSTLFPLAFTIFALFFGAGNLLFPPELGALAGENLVPAMIGFVITGVGLPLLALFAVARVGGGTDQIARYIPKRLALV
LTGLMYVAIGPFFGIPRTAAVTYEMGLVPFLDAPSTLTLAMSSTVFFGLTFFLTLRAQKLIEVIGRIITPILLFLLAAIG
ITNLISPLGEPGAPAEGFETWVGALGQGFQQGYLTMDVFGALVFGAIVLVTLKANGLSDQKEASSLLRSAGMLAVTCLML
VYISLGSIGANMTVAEGTTSGASLLQAFAGTSYGQVGMLALGLAVFLACLTTAVGLISEFSRFISNQFDSLKYHHVVIAT
TLFGFMISLVGLSSLIQIAVPLLTLLYPVMIALVLVSVTERLQRNPGVAMKATIYTALVLSFFETWNGIQPSAVTSWIGS
LPMADIGLAWAVPTMIVYAITLLLPKGTSQESLAQSRIS

Specific function: Component of the LIV-II transport system for branched- chain amino acids. BraB is specific for isoleucine, leucine and valine. The LIV-II transport system is coupled to sodium and lithium ions [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=436, Percent_Identity=34.1743119266055, Blast_Score=252, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 46255; Mature: 46255

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure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HHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2111004; 10984043 [H]