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The map label for this gene is blt [H]

Identifier: 229916522

GI number: 229916522

Start: 746186

End: 747367

Strand: Reverse

Name: blt [H]

Synonym: EAT1b_0792

Alternate gene names: 229916522

Gene position: 747367-746186 (Counterclockwise)

Preceding gene: 229916523

Following gene: 229916521

Centisome position: 24.91

GC content: 49.15

Gene sequence:

>1182_bases
TTGACGACAAAACAAAAAAATCAATTGGCGATTCTCATGTTGAACATGTTTATCGCCGTCGCCGGGTTTGGGATCATCAT
CCCGATTATTCCGGATTACTTAAAGATGATTGGCGAGGGAGGGACAGCCGCCGGACTCATGATTTCCGTATTTGCGGGTG
CCCAACTCGTCATGTCACCGATTGCCGGGAAATGGGCGGACGTCTACGGCCGCCGACTCATGATCATTCTCGGTCTAATC
GGATTCACCATCTCGATGGGAGTCTTCTATCTATCAGATAATTTAACGATTCTTCTCGTCTCTCGTGCCATCGGGGGTGT
TGGTGCAGCGCTTTTGATTCCTGCCATCTTCGCTTATGTGGCAGATATCACGACACTCGAGCAACGAGCTCGAGGAAACA
GTTATATTTCCGCGGCAATGTCGCTTGGAATTGTGGTCGGACCAGGGATTGGGGGCTTTTTTGCTGAATTTGGCTTGAAG
ACACCGTTGCTCGTTTCAGCGATTGTATCGTTTATCGCTGTCATTTCGTCTGTACTCCTTCTTAAGGAGCCGGACCGTGC
TCCGTACGTGGCACCAAAAATAAAAGAATCTTTACCGACACAGCTGGTCATGTCGACGCGAAAACCATACTTCTTCGTAT
TGGTCATCAACCTCGTGATGGCGTTTGGTCTGATGGCGTATGAATCGGTACTCGGACTCTATGTCAGCGAGATGTTCAGT
GCGACACCACAAGAAATTGCCATCATGGTAACCGGAACAGGGATTGTAAGTGTCATCGTTCAATTGTTTGCGGTGGACCG
TCTCGTCGAATGGTTGGGTGAGACCAAGGTCGTGCTCGCTTTCATCGCACTCGCCTCATTTGGCTTCTTGTTATCTTTGT
TCGCGGGCACGTATATGCTCTTCTTCGCAGTGACACTTGTGATTTTCTTATCGACATCCATTTTACGACCGGTGTTGAAC
ACACTCGTGTCGAAACTGGCTGGCAATGAGCAAGGTCTCGCCATGGGGATGAACAACGCCTATATGAGTATCGGGAACAT
TTTAGGACCAACATTGGCGGGAATCGCCTTTGATATCGATATGCGCTATCCGTTCATCATGGGACTCGTCCTGTTGCTCG
TCACATACATGGGCACATGGAACTGGTCGAAGCGGAAAGCGGCCGCCATTTCAGAATCATAA

Upstream 100 bases:

>100_bases
CGCCCATCCTTTGGTCAAGGATGGGTTTTTTCTGTCTTATTGTTTGCCTGGATGTGAACTCAAACAGTAAAATGGAAAGA
TGAAAAGGGAGTGACATGCG

Downstream 100 bases:

>100_bases
AGGAGGACGCTATGATCTACGTAGGTGTGACCGGATGGGGAGACCACGATTTGCTGTATACGACCCCAGAAGAACGAAAA
CATAAATTGTCAACATATGC

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 2 [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTTKQKNQLAILMLNMFIAVAGFGIIIPIIPDYLKMIGEGGTAAGLMISVFAGAQLVMSPIAGKWADVYGRRLMIILGLI
GFTISMGVFYLSDNLTILLVSRAIGGVGAALLIPAIFAYVADITTLEQRARGNSYISAAMSLGIVVGPGIGGFFAEFGLK
TPLLVSAIVSFIAVISSVLLLKEPDRAPYVAPKIKESLPTQLVMSTRKPYFFVLVINLVMAFGLMAYESVLGLYVSEMFS
ATPQEIAIMVTGTGIVSVIVQLFAVDRLVEWLGETKVVLAFIALASFGFLLSLFAGTYMLFFAVTLVIFLSTSILRPVLN
TLVSKLAGNEQGLAMGMNNAYMSIGNILGPTLAGIAFDIDMRYPFIMGLVLLLVTYMGTWNWSKRKAAAISES

Sequences:

>Translated_393_residues
MTTKQKNQLAILMLNMFIAVAGFGIIIPIIPDYLKMIGEGGTAAGLMISVFAGAQLVMSPIAGKWADVYGRRLMIILGLI
GFTISMGVFYLSDNLTILLVSRAIGGVGAALLIPAIFAYVADITTLEQRARGNSYISAAMSLGIVVGPGIGGFFAEFGLK
TPLLVSAIVSFIAVISSVLLLKEPDRAPYVAPKIKESLPTQLVMSTRKPYFFVLVINLVMAFGLMAYESVLGLYVSEMFS
ATPQEIAIMVTGTGIVSVIVQLFAVDRLVEWLGETKVVLAFIALASFGFLLSLFAGTYMLFFAVTLVIFLSTSILRPVLN
TLVSKLAGNEQGLAMGMNNAYMSIGNILGPTLAGIAFDIDMRYPFIMGLVLLLVTYMGTWNWSKRKAAAISES
>Mature_392_residues
TTKQKNQLAILMLNMFIAVAGFGIIIPIIPDYLKMIGEGGTAAGLMISVFAGAQLVMSPIAGKWADVYGRRLMIILGLIG
FTISMGVFYLSDNLTILLVSRAIGGVGAALLIPAIFAYVADITTLEQRARGNSYISAAMSLGIVVGPGIGGFFAEFGLKT
PLLVSAIVSFIAVISSVLLLKEPDRAPYVAPKIKESLPTQLVMSTRKPYFFVLVINLVMAFGLMAYESVLGLYVSEMFSA
TPQEIAIMVTGTGIVSVIVQLFAVDRLVEWLGETKVVLAFIALASFGFLLSLFAGTYMLFFAVTLVIFLSTSILRPVLNT
LVSKLAGNEQGLAMGMNNAYMSIGNILGPTLAGIAFDIDMRYPFIMGLVLLLVTYMGTWNWSKRKAAAISES

Specific function: Energy-dependent efflux pump responsible for decreased drug accumulation in multi-drug-resistant cells. Probably uses a transmembrane proton gradient as the energy source. Causes the efflux of a variety of toxic substances, including such structurally div

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI217416366, Length=410, Percent_Identity=21.4634146341463, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787291, Length=396, Percent_Identity=22.7272727272727, Blast_Score=63, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17738085, Length=359, Percent_Identity=27.0194986072423, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137160, Length=388, Percent_Identity=25.2577319587629, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42143; Mature: 42012

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKQKNQLAILMLNMFIAVAGFGIIIPIIPDYLKMIGEGGTAAGLMISVFAGAQLVMSP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAGKWADVYGRRLMIILGLIGFTISMGVFYLSDNLTILLVSRAIGGVGAALLIPAIFAYV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEHHHHHCCCHHHHHHHHHHHHH
ADITTLEQRARGNSYISAAMSLGIVVGPGIGGFFAEFGLKTPLLVSAIVSFIAVISSVLL
HHHHHHHHHHCCCHHHHHHHHHCEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LKEPDRAPYVAPKIKESLPTQLVMSTRKPYFFVLVINLVMAFGLMAYESVLGLYVSEMFS
HCCCCCCCCCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQEIAIMVTGTGIVSVIVQLFAVDRLVEWLGETKVVLAFIALASFGFLLSLFAGTYML
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAVTLVIFLSTSILRPVLNTLVSKLAGNEQGLAMGMNNAYMSIGNILGPTLAGIAFDID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHEEEEC
MRYPFIMGLVLLLVTYMGTWNWSKRKAAAISES
CCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC
>Mature Secondary Structure 
TTKQKNQLAILMLNMFIAVAGFGIIIPIIPDYLKMIGEGGTAAGLMISVFAGAQLVMSP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
IAGKWADVYGRRLMIILGLIGFTISMGVFYLSDNLTILLVSRAIGGVGAALLIPAIFAYV
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEHHHHHCCCHHHHHHHHHHHHH
ADITTLEQRARGNSYISAAMSLGIVVGPGIGGFFAEFGLKTPLLVSAIVSFIAVISSVLL
HHHHHHHHHHCCCHHHHHHHHHCEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
LKEPDRAPYVAPKIKESLPTQLVMSTRKPYFFVLVINLVMAFGLMAYESVLGLYVSEMFS
HCCCCCCCCCCHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ATPQEIAIMVTGTGIVSVIVQLFAVDRLVEWLGETKVVLAFIALASFGFLLSLFAGTYML
CCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FFAVTLVIFLSTSILRPVLNTLVSKLAGNEQGLAMGMNNAYMSIGNILGPTLAGIAFDID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHEEEEC
MRYPFIMGLVLLLVTYMGTWNWSKRKAAAISES
CCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7608059; 8969508; 9384377 [H]