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The map label for this gene is nrdF [H]

Identifier: 229916488

GI number: 229916488

Start: 711710

End: 712750

Strand: Direct

Name: nrdF [H]

Synonym: EAT1b_0758

Alternate gene names: 229916488

Gene position: 711710-712750 (Clockwise)

Preceding gene: 229916487

Following gene: 229916491

Centisome position: 23.72

GC content: 46.3

Gene sequence:

>1041_bases
ATGTCGATTCAGAAAATCAAATTATTGTCGCCCAAACATCCTAACCGGGCAACCGCCATCATTGGCGGGGAAACGAGTTC
GATCGTCAATTGGAACGATATCGCGTATCCTCAGTTCTATACCATTTACAAACAGTTGTTATCGAACTTTTGGATTCCTG
ATGAGATCTCCATGTCTAAGGATATGCAACAATGGCCGCAATTGACTGAACGTGAACAGGATGCATTCAAACGGATCATC
GGACTGCTCTCGATTCTCGACTCGGTCCAGACACGATACATCCTCGAGTCTTCCATGTTCTCATCTGACTCATCTGTCCA
TGCCGTTCTCGCGGTCATCGCACAACAAGAGGTCGTGCACAATCAATCATACAGCTATGTCCTATCAAGTCTCGTGCCGC
TCGCTGAACAAAATCGAATCTTCAACATCGCTAAAGACGATGAGATGGTCATGAAGCGCAATCAATTCATCTTGGACTTA
TATGAAGCGTTCCGCGAGACTCCGAACGCCGAAAACTTTGCGAAATCGCTCGTCGCATCTGTCATTTTAGAAGGAATCAA
CTTCTATTCAGGCTTCGCCTACTTCTATAACCTTGCCCGTCACCAAAAGATGGTGGGGACATCCACGATGATCAGTTATA
TCCAGCGCGACGAAATGCAGCATTCTTACTTCATCAGCCAGCTCTTACGTGCCGTATTGACAGAAAATCCTAAGATTGAT
GAAAACGGACAGTTCAGTCAGTTCGTATACGACACGATGGCGGAAGCGGTCGATCTCGAGATCGAGTGGTGTGAATATGT
ACTACGCGACCTTGACGGACTCGACGTCAGTGAAATGCGAGACTACGTCAAGTACTTGGCGAACAAACGCCTCCGCGTCA
TCGGTCTGAATGACTTGTATGAAGGATTTGACGAAGATGTCATGCCTTGGATCCGTGCATACTCAGATGACTCGCTCAAT
GCGACAAAATCTGATTTCTTCGAACAAAAGTCACGTTCTTATGCGAAAGTGACAGACGCGAACGGATTTGATGATCTATA
A

Upstream 100 bases:

>100_bases
GAACTGCTTAAAATCGAGCAGTCCCCACGTAACCAACCGTTCAACGTCCAACATTGGGCAGAGCGGATGGCTCACCATGT
AGAAGAAGGAGTTATGCATC

Downstream 100 bases:

>100_bases
AAAAACTCCCCGATGCTCTCAAAGCATCGGGGAGTTTGCTCTTTATTTATTTGCCTTTAAGTATTCCACGATTCCTTCCG
CGACACCTTCGGCCATTTTT

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSIQKIKLLSPKHPNRATAIIGGETSSIVNWNDIAYPQFYTIYKQLLSNFWIPDEISMSKDMQQWPQLTEREQDAFKRII
GLLSILDSVQTRYILESSMFSSDSSVHAVLAVIAQQEVVHNQSYSYVLSSLVPLAEQNRIFNIAKDDEMVMKRNQFILDL
YEAFRETPNAENFAKSLVASVILEGINFYSGFAYFYNLARHQKMVGTSTMISYIQRDEMQHSYFISQLLRAVLTENPKID
ENGQFSQFVYDTMAEAVDLEIEWCEYVLRDLDGLDVSEMRDYVKYLANKRLRVIGLNDLYEGFDEDVMPWIRAYSDDSLN
ATKSDFFEQKSRSYAKVTDANGFDDL

Sequences:

>Translated_346_residues
MSIQKIKLLSPKHPNRATAIIGGETSSIVNWNDIAYPQFYTIYKQLLSNFWIPDEISMSKDMQQWPQLTEREQDAFKRII
GLLSILDSVQTRYILESSMFSSDSSVHAVLAVIAQQEVVHNQSYSYVLSSLVPLAEQNRIFNIAKDDEMVMKRNQFILDL
YEAFRETPNAENFAKSLVASVILEGINFYSGFAYFYNLARHQKMVGTSTMISYIQRDEMQHSYFISQLLRAVLTENPKID
ENGQFSQFVYDTMAEAVDLEIEWCEYVLRDLDGLDVSEMRDYVKYLANKRLRVIGLNDLYEGFDEDVMPWIRAYSDDSLN
ATKSDFFEQKSRSYAKVTDANGFDDL
>Mature_345_residues
SIQKIKLLSPKHPNRATAIIGGETSSIVNWNDIAYPQFYTIYKQLLSNFWIPDEISMSKDMQQWPQLTEREQDAFKRIIG
LLSILDSVQTRYILESSMFSSDSSVHAVLAVIAQQEVVHNQSYSYVLSSLVPLAEQNRIFNIAKDDEMVMKRNQFILDLY
EAFRETPNAENFAKSLVASVILEGINFYSGFAYFYNLARHQKMVGTSTMISYIQRDEMQHSYFISQLLRAVLTENPKIDE
NGQFSQFVYDTMAEAVDLEIEWCEYVLRDLDGLDVSEMRDYVKYLANKRLRVIGLNDLYEGFDEDVMPWIRAYSDDSLNA
TKSDFFEQKSRSYAKVTDANGFDDL

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI289177074, Length=308, Percent_Identity=23.0519480519481, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI42544136, Length=308, Percent_Identity=23.0519480519481, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4557845, Length=343, Percent_Identity=21.865889212828, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI260064013, Length=343, Percent_Identity=21.865889212828, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789031, Length=311, Percent_Identity=28.2958199356913, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1788567, Length=322, Percent_Identity=25.4658385093168, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17551968, Length=311, Percent_Identity=24.7588424437299, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6322434, Length=314, Percent_Identity=23.8853503184713, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18079279, Length=309, Percent_Identity=22.9773462783171, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 40045; Mature: 39913

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQKIKLLSPKHPNRATAIIGGETSSIVNWNDIAYPQFYTIYKQLLSNFWIPDEISMSK
CCCCCEECCCCCCCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
DMQQWPQLTEREQDAFKRIIGLLSILDSVQTRYILESSMFSSDSSVHAVLAVIAQQEVVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NQSYSYVLSSLVPLAEQNRIFNIAKDDEMVMKRNQFILDLYEAFRETPNAENFAKSLVAS
CCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VILEGINFYSGFAYFYNLARHQKMVGTSTMISYIQRDEMQHSYFISQLLRAVLTENPKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ENGQFSQFVYDTMAEAVDLEIEWCEYVLRDLDGLDVSEMRDYVKYLANKRLRVIGLNDLY
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECHHHH
EGFDEDVMPWIRAYSDDSLNATKSDFFEQKSRSYAKVTDANGFDDL
HCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEECCCCCCCCC
>Mature Secondary Structure 
SIQKIKLLSPKHPNRATAIIGGETSSIVNWNDIAYPQFYTIYKQLLSNFWIPDEISMSK
CCCCEECCCCCCCCCCEEEECCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
DMQQWPQLTEREQDAFKRIIGLLSILDSVQTRYILESSMFSSDSSVHAVLAVIAQQEVVH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
NQSYSYVLSSLVPLAEQNRIFNIAKDDEMVMKRNQFILDLYEAFRETPNAENFAKSLVAS
CCHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
VILEGINFYSGFAYFYNLARHQKMVGTSTMISYIQRDEMQHSYFISQLLRAVLTENPKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
ENGQFSQFVYDTMAEAVDLEIEWCEYVLRDLDGLDVSEMRDYVKYLANKRLRVIGLNDLY
CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCEEEEECHHHH
EGFDEDVMPWIRAYSDDSLNATKSDFFEQKSRSYAKVTDANGFDDL
HCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]