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The map label for this gene is pckA [H]

Identifier: 229916473

GI number: 229916473

Start: 696855

End: 698426

Strand: Direct

Name: pckA [H]

Synonym: EAT1b_0743

Alternate gene names: 229916473

Gene position: 696855-698426 (Clockwise)

Preceding gene: 229916471

Following gene: 229916486

Centisome position: 23.23

GC content: 50.38

Gene sequence:

>1572_bases
ATGGCGACAACGCTCCTGAAAATCGAAGAATTAATTAATGGTGCCGACATGTTACGGAACTTGACGGTTCCAGAGCTGGT
GGAAGAAGCCATTCAAAATGGTGAAGGTGTACTGACAAAAGACGGGGCATTATCTGTCAGCACCGGTGCTTTTACTGGAC
GGTCACCGAAAGACAAATTCGTCGTACAAGATGAAGTCAGCAACAACCTCGTCGATTGGGGTAAAGTGAACCAACCGATC
GCAGAAGAGAAGTTTTCAGCACTTCTTGACAAAGTACTCGACCATTTAGAAAAGAAGGATAAGCTGTATTATCTAGAAGC
ATCCGCTGGTGCAGATGAACACTATACGTTGCCGGTCCGTGCCATTACGGAGTATGCGTGGCACAGTCTGTTCGCCAAAC
AATTGTTCTTACGCGAATGGCCGACGAAAGGTGCGTTCGACCCGTTCACGGTCATTTATGCACCGAGCTATAAAGCAGAC
CCTGCGGTCGATGGTACGAACTCTGAGACGTTCATTCTCGTCTCATTCGCCAAGCGCACGATTCTGATCGGGGGAACAGA
ATACGGTGGCGAAATTAAAAAATCGATCTTCTCCGTCATGAACTTCTTGCTACCTGAGCAGAACGTGTTATCGATGCACT
GTTCGGCAAACGTCAACGATGCGAACGAAACGGCACTCTTCTTCGGATTGTCGGGGACTGGGAAGACAACGCTCTCGGCT
GACCCTGAGCGTCACTTGATTGGAGACGACGAACACGGATGGTCACCGGACGGGGTCTTCAACATCGAAGGCGGTTGTTA
CGCGAAGACGATCAACCTGTCTCGTGAGAACGAACCTCAAATCTATGATGCGATTCGCTTCGGTTCATTGATTGAAAACG
TCGTGTTGAATGAAGATCGTACACCAGACTATAAAAATATTTCCTTGACGGAAAACACACGTGCGGCATATCCAATCGAG
TTCATCGACAATGCGATGCTCCCATCACGCGCCGGTCATCCGAATACGATCGTCTTCTTGACGGCAGATGCCTATGGCGT
CTTGCCTCCGATCAGCAAACTGACGAAAGAACAGGCGATGTACCACTTCTTGTCGGGTTATACGTCTAAGCTTGCTGGTA
CGGAGCGCGGTGTTACAGAACCACAGGCGACCTTCTCGACTTGCTTCGGTTCACCGTTCCTTCCGCTCGCTCCAGAGCGT
TACGCGGATATGCTCGGTAAATTGATTGAGGAACACGACGTGGATGTCTACCTCGTCAACACAGGTTGGACTGGCGGTGC
ATACGGTGAAGGTTCACGTATGAAACTCGCGTACACGCGTGCGATGGTCAACGCCGCAGTCAACGGTCAACTTCGTGACG
TCGAGACGATGAAACATGACATTTTTAACGTCGAGATTCCAGTAGCCATCGAAGGCGTACCGAGTGACGTGCTCAATCCA
CGTGACACGTGGAACGACCCAGCTCGCTACGACGTCGAGGCGAAAGCACTCGCAATGAAGTTTAAAGAGAACTTCACACG
GTTCACGAAAGCGAGTGAATCGGTCATCGCAGCCGGTCCACTGGTCATCTAA

Upstream 100 bases:

>100_bases
AGTGTGTCACATATAATTAAAAATGTCATACTGATTAATCGTAATCGCGGAAGTATGCGGATTTCATCCACATCTATTTA
GGGGAAAGGTGGGATTCAGG

Downstream 100 bases:

>100_bases
TACGAAAGCACCGACTCATCGAGTCGGTGCTTTCGCTTGTTGTAGCATCCATTCCATCGCTTGTTCGGTCGTCTCCCATT
TATCCCAAAACGGGAGTTGG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MATTLLKIEELINGADMLRNLTVPELVEEAIQNGEGVLTKDGALSVSTGAFTGRSPKDKFVVQDEVSNNLVDWGKVNQPI
AEEKFSALLDKVLDHLEKKDKLYYLEASAGADEHYTLPVRAITEYAWHSLFAKQLFLREWPTKGAFDPFTVIYAPSYKAD
PAVDGTNSETFILVSFAKRTILIGGTEYGGEIKKSIFSVMNFLLPEQNVLSMHCSANVNDANETALFFGLSGTGKTTLSA
DPERHLIGDDEHGWSPDGVFNIEGGCYAKTINLSRENEPQIYDAIRFGSLIENVVLNEDRTPDYKNISLTENTRAAYPIE
FIDNAMLPSRAGHPNTIVFLTADAYGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPQATFSTCFGSPFLPLAPER
YADMLGKLIEEHDVDVYLVNTGWTGGAYGEGSRMKLAYTRAMVNAAVNGQLRDVETMKHDIFNVEIPVAIEGVPSDVLNP
RDTWNDPARYDVEAKALAMKFKENFTRFTKASESVIAAGPLVI

Sequences:

>Translated_523_residues
MATTLLKIEELINGADMLRNLTVPELVEEAIQNGEGVLTKDGALSVSTGAFTGRSPKDKFVVQDEVSNNLVDWGKVNQPI
AEEKFSALLDKVLDHLEKKDKLYYLEASAGADEHYTLPVRAITEYAWHSLFAKQLFLREWPTKGAFDPFTVIYAPSYKAD
PAVDGTNSETFILVSFAKRTILIGGTEYGGEIKKSIFSVMNFLLPEQNVLSMHCSANVNDANETALFFGLSGTGKTTLSA
DPERHLIGDDEHGWSPDGVFNIEGGCYAKTINLSRENEPQIYDAIRFGSLIENVVLNEDRTPDYKNISLTENTRAAYPIE
FIDNAMLPSRAGHPNTIVFLTADAYGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPQATFSTCFGSPFLPLAPER
YADMLGKLIEEHDVDVYLVNTGWTGGAYGEGSRMKLAYTRAMVNAAVNGQLRDVETMKHDIFNVEIPVAIEGVPSDVLNP
RDTWNDPARYDVEAKALAMKFKENFTRFTKASESVIAAGPLVI
>Mature_522_residues
ATTLLKIEELINGADMLRNLTVPELVEEAIQNGEGVLTKDGALSVSTGAFTGRSPKDKFVVQDEVSNNLVDWGKVNQPIA
EEKFSALLDKVLDHLEKKDKLYYLEASAGADEHYTLPVRAITEYAWHSLFAKQLFLREWPTKGAFDPFTVIYAPSYKADP
AVDGTNSETFILVSFAKRTILIGGTEYGGEIKKSIFSVMNFLLPEQNVLSMHCSANVNDANETALFFGLSGTGKTTLSAD
PERHLIGDDEHGWSPDGVFNIEGGCYAKTINLSRENEPQIYDAIRFGSLIENVVLNEDRTPDYKNISLTENTRAAYPIEF
IDNAMLPSRAGHPNTIVFLTADAYGVLPPISKLTKEQAMYHFLSGYTSKLAGTERGVTEPQATFSTCFGSPFLPLAPERY
ADMLGKLIEEHDVDVYLVNTGWTGGAYGEGSRMKLAYTRAMVNAAVNGQLRDVETMKHDIFNVEIPVAIEGVPSDVLNPR
DTWNDPARYDVEAKALAMKFKENFTRFTKASESVIAAGPLVI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=500, Percent_Identity=48.8, Blast_Score=473, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6322950, Length=514, Percent_Identity=47.4708171206226, Blast_Score=467, Evalue=1e-132,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 57658; Mature: 57526

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTLLKIEELINGADMLRNLTVPELVEEAIQNGEGVLTKDGALSVSTGAFTGRSPKDKF
CCCHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCE
VVQDEVSNNLVDWGKVNQPIAEEKFSALLDKVLDHLEKKDKLYYLEASAGADEHYTLPVR
EEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHH
AITEYAWHSLFAKQLFLREWPTKGAFDPFTVIYAPSYKADPAVDGTNSETFILVSFAKRT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEE
ILIGGTEYGGEIKKSIFSVMNFLLPEQNVLSMHCSANVNDANETALFFGLSGTGKTTLSA
EEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEECC
DPERHLIGDDEHGWSPDGVFNIEGGCYAKTINLSRENEPQIYDAIRFGSLIENVVLNEDR
CCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TPDYKNISLTENTRAAYPIEFIDNAMLPSRAGHPNTIVFLTADAYGVLPPISKLTKEQAM
CCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
YHFLSGYTSKLAGTERGVTEPQATFSTCFGSPFLPLAPERYADMLGKLIEEHDVDVYLVN
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
TGWTGGAYGEGSRMKLAYTRAMVNAAVNGQLRDVETMKHDIFNVEIPVAIEGVPSDVLNP
CCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEEEECCCHHHCCC
RDTWNDPARYDVEAKALAMKFKENFTRFTKASESVIAAGPLVI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
>Mature Secondary Structure 
ATTLLKIEELINGADMLRNLTVPELVEEAIQNGEGVLTKDGALSVSTGAFTGRSPKDKF
CCHHHHHHHHHCHHHHHHCCCHHHHHHHHHHCCCCEEEECCCEEEECCCCCCCCCCCCE
VVQDEVSNNLVDWGKVNQPIAEEKFSALLDKVLDHLEKKDKLYYLEASAGADEHYTLPVR
EEEEHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCEEEHHH
AITEYAWHSLFAKQLFLREWPTKGAFDPFTVIYAPSYKADPAVDGTNSETFILVSFAKRT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCEE
ILIGGTEYGGEIKKSIFSVMNFLLPEQNVLSMHCSANVNDANETALFFGLSGTGKTTLSA
EEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEECCCCCCEECC
DPERHLIGDDEHGWSPDGVFNIEGGCYAKTINLSRENEPQIYDAIRFGSLIENVVLNEDR
CCCCCCCCCCCCCCCCCCEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCC
TPDYKNISLTENTRAAYPIEFIDNAMLPSRAGHPNTIVFLTADAYGVLPPISKLTKEQAM
CCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHH
YHFLSGYTSKLAGTERGVTEPQATFSTCFGSPFLPLAPERYADMLGKLIEEHDVDVYLVN
HHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
TGWTGGAYGEGSRMKLAYTRAMVNAAVNGQLRDVETMKHDIFNVEIPVAIEGVPSDVLNP
CCCCCCCCCCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHEEEEEEEEEECCCHHHCCC
RDTWNDPARYDVEAKALAMKFKENFTRFTKASESVIAAGPLVI
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]