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The map label for this gene is glyS [H]

Identifier: 229916324

GI number: 229916324

Start: 544165

End: 546219

Strand: Reverse

Name: glyS [H]

Synonym: EAT1b_0593

Alternate gene names: 229916324

Gene position: 546219-544165 (Counterclockwise)

Preceding gene: 229916325

Following gene: 229916323

Centisome position: 18.21

GC content: 48.52

Gene sequence:

>2055_bases
ATGCGTGACCTTCTGTTAGAAATTGGATTAGAGGAACTGCCGGCTCAATACGTCTTGCGTTCAGAGAAGCAGTTGGCGGA
ACGTGTTGACGCGTTCTTAAAAGAGGCGCGTGTTGATTTTGGCTCAATCGAAGTGTACTCGACACCACGTCGTTTGGCTG
TCATCGTTCGGGATGTGGCTGAAACGCAACAAGATTTGACAGAAACCCTTCGTGGACCTGCCAAAAAGATTGCACAAGAT
GCGGAAGGTAACTGGTCGAAAGCGGCACAAGGTTTTGCGCGTGGCCGCGGATTGACAGTGGAAGATCTCTATTTCGCAGA
AGAAAAAGGAGTCGAGTACGTCTTTGCAGAGCGTCATGAAAAAGGAAAAGAAACAGTTTCGTTATTGAACGGTCTTGAAG
ATGTGATCCGTAGCTTGACGTTCCCGAAAAACATGAAATGGGGAACGAGCAGCCTACGGTATATGCGCCCGATTCGTTGG
TTGATCGCCCTCTTCGGTCAAGACATCATCGACTTCACAATCGAAGGCGTGAACACGAATAACGTGACGCGTGGGCATCG
TTTCCTCAGTAAAGGGGACGTGGTCATCTCATCACCTGCCGAATATGTCCAGACATTAGAGTCTGCTTTTGTTATGGCGA
ACTACGAGATGCGTAAAGCTGAAATCGAACGTCAAATTCAAGAGCTTGCCACGAAACAAGGATGGACGATCCCACTTGAT
GCCGACTTGCTCGAAGAAGTGACAAACTTAGTCGAATGGCCGACCGCTCTCTTCGGGGAATTTGATGAAGCGTACCTCGA
CTTGCCTGAGGAAGTATTGATCACAACGATGAAAGAGCATCAACGTTATTTCCCGGTCTATCTCGACGGAACGTTGAAAA
ATTATTTTGTGACGGTACGAAACGGGAACGCCGAGCACCTTGAAAATGTAGCGCGCGGTAACGAGAAAGTTATTCGGGCC
CGTCTTGCGGATGCAGTGTTCTTCTATGAAGAAGACAAAAAGACGAAAATCGATGATCAACTCGGACGTCTCGACCGCAT
CGTCTTCCATGAAAAATTGGGAACGACTGGTGCGAAAGCACGTCGCGTGACGAAAATCGCCAAAGAACTTGCGGCGTTGT
TTGAGGCAGACCTTGAAAAAGTAAGCCGTGCCGGTGCCATTCATAAATTTGACCTCGTGAGCCAGATGGTATACGAGTTC
ACAGAACTTCAAGGGATCATGGGTGAGAAGTATGCACTCCAACAAGGCGAAGACAAGGAAGTGGCAGCGGCGATCCGTGA
GCATTACATGCCACGCTTTGCTGGGGACGCTTCTCCAGAGACGGCAACAGGTGCCGTTCTTGCCCTTGCCGATAAATTAG
ACTCGATTGCAGCATTCTTTGGTATCGGGATGATTCCGAGCGGTTCACAAGACCCGTTCGCGCTTCGTCGCCAGGCCCAA
GGTGTCGTTCAAATTCTTGGAGATTGGAAGATTGACCTCTCAATCGACACGTTGCTTTCAAAAGTTGTCGAATTGCAACT
GGAAGCAGGACTCTATGAAGCCGATAAAGAACAAGTTAAGGCACAACTAGATGATTTCTTCAAACTGCGCTTGAAATATC
GCCTGCAAGAACAGCAGGTTCGTTATGATGTGATTGACGCCGTATTGACGACCTCATTGACGGTTGTGCGACTCGATGCC
CGCGCAGACGCGATTCAAGAATTTGTATCAACGGATGCGAAGGCATTGATTGAGCAATTGTCGCGTGTCGTGAACATCTC
GAAAAAAGGTGAAGCGGGAGACGTCGATCCTTCATTGTTTGAGAACGATCATGAGATTGCGTTACATGATGCGATTGAAC
AGACCGCTCCTGCAACAGCCGATGCTGTCGAGATGGGAGATTACAAGAAAGCGCTCGACCTTCTACAACAACTCGAATCA
CCGATTATGATGTATTTCGAACATACAATGGTTATGAGTGATGACGAGGCGGTCCGCCAAAACCGTCTCCATGAAATGAA
CCGACTTGCAACGACGATTAAGCGTGTCGCAGATTTCAACGCGCTCGTACTCTAA

Upstream 100 bases:

>100_bases
CGTGTCCGGAACATGTCCCGTGCGTGTGCCGCTAAATTTATTGAAGAACGGGAACGCCTTGGATTCCCACTTTTGAAACA
AGAGAAAGCAGGTGACTTGA

Downstream 100 bases:

>100_bases
ACAAACTAGAGGGGGTTGGCTATGCAACTGAATGAACGCCAACAAAAAATCGTCGAGATCGTGAAGGAGAACGGGCCGAT
CACAGGGGAACAAATCGCAC

Product: glycyl-tRNA synthetase, subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 684; Mature: 684

Protein sequence:

>684_residues
MRDLLLEIGLEELPAQYVLRSEKQLAERVDAFLKEARVDFGSIEVYSTPRRLAVIVRDVAETQQDLTETLRGPAKKIAQD
AEGNWSKAAQGFARGRGLTVEDLYFAEEKGVEYVFAERHEKGKETVSLLNGLEDVIRSLTFPKNMKWGTSSLRYMRPIRW
LIALFGQDIIDFTIEGVNTNNVTRGHRFLSKGDVVISSPAEYVQTLESAFVMANYEMRKAEIERQIQELATKQGWTIPLD
ADLLEEVTNLVEWPTALFGEFDEAYLDLPEEVLITTMKEHQRYFPVYLDGTLKNYFVTVRNGNAEHLENVARGNEKVIRA
RLADAVFFYEEDKKTKIDDQLGRLDRIVFHEKLGTTGAKARRVTKIAKELAALFEADLEKVSRAGAIHKFDLVSQMVYEF
TELQGIMGEKYALQQGEDKEVAAAIREHYMPRFAGDASPETATGAVLALADKLDSIAAFFGIGMIPSGSQDPFALRRQAQ
GVVQILGDWKIDLSIDTLLSKVVELQLEAGLYEADKEQVKAQLDDFFKLRLKYRLQEQQVRYDVIDAVLTTSLTVVRLDA
RADAIQEFVSTDAKALIEQLSRVVNISKKGEAGDVDPSLFENDHEIALHDAIEQTAPATADAVEMGDYKKALDLLQQLES
PIMMYFEHTMVMSDDEAVRQNRLHEMNRLATTIKRVADFNALVL

Sequences:

>Translated_684_residues
MRDLLLEIGLEELPAQYVLRSEKQLAERVDAFLKEARVDFGSIEVYSTPRRLAVIVRDVAETQQDLTETLRGPAKKIAQD
AEGNWSKAAQGFARGRGLTVEDLYFAEEKGVEYVFAERHEKGKETVSLLNGLEDVIRSLTFPKNMKWGTSSLRYMRPIRW
LIALFGQDIIDFTIEGVNTNNVTRGHRFLSKGDVVISSPAEYVQTLESAFVMANYEMRKAEIERQIQELATKQGWTIPLD
ADLLEEVTNLVEWPTALFGEFDEAYLDLPEEVLITTMKEHQRYFPVYLDGTLKNYFVTVRNGNAEHLENVARGNEKVIRA
RLADAVFFYEEDKKTKIDDQLGRLDRIVFHEKLGTTGAKARRVTKIAKELAALFEADLEKVSRAGAIHKFDLVSQMVYEF
TELQGIMGEKYALQQGEDKEVAAAIREHYMPRFAGDASPETATGAVLALADKLDSIAAFFGIGMIPSGSQDPFALRRQAQ
GVVQILGDWKIDLSIDTLLSKVVELQLEAGLYEADKEQVKAQLDDFFKLRLKYRLQEQQVRYDVIDAVLTTSLTVVRLDA
RADAIQEFVSTDAKALIEQLSRVVNISKKGEAGDVDPSLFENDHEIALHDAIEQTAPATADAVEMGDYKKALDLLQQLES
PIMMYFEHTMVMSDDEAVRQNRLHEMNRLATTIKRVADFNALVL
>Mature_684_residues
MRDLLLEIGLEELPAQYVLRSEKQLAERVDAFLKEARVDFGSIEVYSTPRRLAVIVRDVAETQQDLTETLRGPAKKIAQD
AEGNWSKAAQGFARGRGLTVEDLYFAEEKGVEYVFAERHEKGKETVSLLNGLEDVIRSLTFPKNMKWGTSSLRYMRPIRW
LIALFGQDIIDFTIEGVNTNNVTRGHRFLSKGDVVISSPAEYVQTLESAFVMANYEMRKAEIERQIQELATKQGWTIPLD
ADLLEEVTNLVEWPTALFGEFDEAYLDLPEEVLITTMKEHQRYFPVYLDGTLKNYFVTVRNGNAEHLENVARGNEKVIRA
RLADAVFFYEEDKKTKIDDQLGRLDRIVFHEKLGTTGAKARRVTKIAKELAALFEADLEKVSRAGAIHKFDLVSQMVYEF
TELQGIMGEKYALQQGEDKEVAAAIREHYMPRFAGDASPETATGAVLALADKLDSIAAFFGIGMIPSGSQDPFALRRQAQ
GVVQILGDWKIDLSIDTLLSKVVELQLEAGLYEADKEQVKAQLDDFFKLRLKYRLQEQQVRYDVIDAVLTTSLTVVRLDA
RADAIQEFVSTDAKALIEQLSRVVNISKKGEAGDVDPSLFENDHEIALHDAIEQTAPATADAVEMGDYKKALDLLQQLES
PIMMYFEHTMVMSDDEAVRQNRLHEMNRLATTIKRVADFNALVL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=38.2608695652174, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 77239; Mature: 77239

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLLLEIGLEELPAQYVLRSEKQLAERVDAFLKEARVDFGSIEVYSTPRRLAVIVRDVA
CCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
ETQQDLTETLRGPAKKIAQDAEGNWSKAAQGFARGRGLTVEDLYFAEEKGVEYVFAERHE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHH
KGKETVSLLNGLEDVIRSLTFPKNMKWGTSSLRYMRPIRWLIALFGQDIIDFTIEGVNTN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCCC
NVTRGHRFLSKGDVVISSPAEYVQTLESAFVMANYEMRKAEIERQIQELATKQGWTIPLD
CCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
ADLLEEVTNLVEWPTALFGEFDEAYLDLPEEVLITTMKEHQRYFPVYLDGTLKNYFVTVR
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCEEEEEEEE
NGNAEHLENVARGNEKVIRARLADAVFFYEEDKKTKIDDQLGRLDRIVFHEKLGTTGAKA
CCCHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
RRVTKIAKELAALFEADLEKVSRAGAIHKFDLVSQMVYEFTELQGIMGEKYALQQGEDKE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHH
VAAAIREHYMPRFAGDASPETATGAVLALADKLDSIAAFFGIGMIPSGSQDPFALRRQAQ
HHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GVVQILGDWKIDLSIDTLLSKVVELQLEAGLYEADKEQVKAQLDDFFKLRLKYRLQEQQV
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYDVIDAVLTTSLTVVRLDARADAIQEFVSTDAKALIEQLSRVVNISKKGEAGDVDPSLF
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ENDHEIALHDAIEQTAPATADAVEMGDYKKALDLLQQLESPIMMYFEHTMVMSDDEAVRQ
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHH
NRLHEMNRLATTIKRVADFNALVL
HHHHHHHHHHHHHHHHHHCHHHCC
>Mature Secondary Structure
MRDLLLEIGLEELPAQYVLRSEKQLAERVDAFLKEARVDFGSIEVYSTPRRLAVIVRDVA
CCHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHH
ETQQDLTETLRGPAKKIAQDAEGNWSKAAQGFARGRGLTVEDLYFAEEKGVEYVFAERHE
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHH
KGKETVSLLNGLEDVIRSLTFPKNMKWGTSSLRYMRPIRWLIALFGQDIIDFTIEGVNTN
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHEEECCCCC
NVTRGHRFLSKGDVVISSPAEYVQTLESAFVMANYEMRKAEIERQIQELATKQGWTIPLD
CCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCC
ADLLEEVTNLVEWPTALFGEFDEAYLDLPEEVLITTMKEHQRYFPVYLDGTLKNYFVTVR
HHHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCEEEEEEEE
NGNAEHLENVARGNEKVIRARLADAVFFYEEDKKTKIDDQLGRLDRIVFHEKLGTTGAKA
CCCHHHHHHHHCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
RRVTKIAKELAALFEADLEKVSRAGAIHKFDLVSQMVYEFTELQGIMGEKYALQQGEDKE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCHH
VAAAIREHYMPRFAGDASPETATGAVLALADKLDSIAAFFGIGMIPSGSQDPFALRRQAQ
HHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
GVVQILGDWKIDLSIDTLLSKVVELQLEAGLYEADKEQVKAQLDDFFKLRLKYRLQEQQV
HHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RYDVIDAVLTTSLTVVRLDARADAIQEFVSTDAKALIEQLSRVVNISKKGEAGDVDPSLF
HHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHH
ENDHEIALHDAIEQTAPATADAVEMGDYKKALDLLQQLESPIMMYFEHTMVMSDDEAVRQ
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHH
NRLHEMNRLATTIKRVADFNALVL
HHHHHHHHHHHHHHHHHHCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]