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The map label for this gene is yqhL [H]

Identifier: 229916264

GI number: 229916264

Start: 485250

End: 485630

Strand: Direct

Name: yqhL [H]

Synonym: EAT1b_0533

Alternate gene names: 229916264

Gene position: 485250-485630 (Clockwise)

Preceding gene: 229916261

Following gene: 229916277

Centisome position: 16.18

GC content: 46.46

Gene sequence:

>381_bases
ATGGGACTCGAAACAATCCTCGTCATCCTCCTATGGGTTGCCTTGATCGCCTACATCGTATGGCGTTTCATGCCAGCTCG
CGGGTTGAAAAAAATGAAACAAGAAGAATTCCGCGCCTCACTCCGTAAAGGTCAGTTGATTGACGTGCGTGAACCGAACG
AATACAAAGGTGGACATATCGTTGGTGCTCGGAATATCCCGGTCGGCCAAATGAAACTACGCATGAAAGAACTGCGCAAA
GATCAGCCGATTTTGATTTACTGCCAAGGTAGTTCACGTTCAAACCAGGCCGCTAAACTCCTGATGAAGAACGGATACAA
CAACATCTACATGCTCGAAGGCGGTTTCAAGAACTGGAAGGGTAAGATTAAAAAAGCATAA

Upstream 100 bases:

>100_bases
GAAAGGGAAAGCCCCGATTGATTGAGTCAATCATTGTCTCATGACCTTAAATTCATTATAATGAGATATGGTAACTTTGA
AAGAGAAGGGAAGAACATCT

Downstream 100 bases:

>100_bases
AAAAACGGGCGGGTCGATTCAATCATCGACTCGCCCATTTTTATTTTACAGATGACGCCTCACGACGCTCATATTTTAAG
ACCGGTTTACGGGCAGCGAG

Product: Rhodanese domain protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MGLETILVILLWVALIAYIVWRFMPARGLKKMKQEEFRASLRKGQLIDVREPNEYKGGHIVGARNIPVGQMKLRMKELRK
DQPILIYCQGSSRSNQAAKLLMKNGYNNIYMLEGGFKNWKGKIKKA

Sequences:

>Translated_126_residues
MGLETILVILLWVALIAYIVWRFMPARGLKKMKQEEFRASLRKGQLIDVREPNEYKGGHIVGARNIPVGQMKLRMKELRK
DQPILIYCQGSSRSNQAAKLLMKNGYNNIYMLEGGFKNWKGKIKKA
>Mature_125_residues
GLETILVILLWVALIAYIVWRFMPARGLKKMKQEEFRASLRKGQLIDVREPNEYKGGHIVGARNIPVGQMKLRMKELRKD
QPILIYCQGSSRSNQAAKLLMKNGYNNIYMLEGGFKNWKGKIKKA

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 14538; Mature: 14406

Theoretical pI: Translated: 10.91; Mature: 10.91

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLETILVILLWVALIAYIVWRFMPARGLKKMKQEEFRASLRKGQLIDVREPNEYKGGHI
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEE
VGARNIPVGQMKLRMKELRKDQPILIYCQGSSRSNQAAKLLMKNGYNNIYMLEGGFKNWK
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCC
GKIKKA
CCCCCC
>Mature Secondary Structure 
GLETILVILLWVALIAYIVWRFMPARGLKKMKQEEFRASLRKGQLIDVREPNEYKGGHI
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCEE
VGARNIPVGQMKLRMKELRKDQPILIYCQGSSRSNQAAKLLMKNGYNNIYMLEGGFKNWK
EECCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCC
GKIKKA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]