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The map label for this gene is nudF [H]

Identifier: 229916188

GI number: 229916188

Start: 418219

End: 418749

Strand: Reverse

Name: nudF [H]

Synonym: EAT1b_0457

Alternate gene names: 229916188

Gene position: 418749-418219 (Counterclockwise)

Preceding gene: 229916190

Following gene: 229916187

Centisome position: 13.96

GC content: 46.33

Gene sequence:

>531_bases
ATGGAAGAAAAAACGATTGAGCGGGAAGTCATTTATCAAGGTAAAATCTTCGATGTGGAAAAGCATGTCGTTTCCCTTCC
GAACGGAAACACGTCCGTACGTGAACTCGTCTATCATAACGGAGCCGTCGCGGTACTCGTCATCGATGAATCGGACAACA
TCGTCATGGTGGAGCAGTACCGAAAAGCCTTTGAATCGATGTCGCTTGAGATCCCTGCCGGTAAGTTGGAAAAAGGGGAA
GAGCCGTTGGCGAGTGCTGAGCGCGAACTCGAAGAAGAGACAGGTTATACGGCGGAATCGCTTGAAAAGATTTTTTCTTT
CTACGGCGCACCTGGGTTCTGCAGTGAGCGGGTCGATGTATATGTCGCAAAAGGGTTATCTGCCGGTGAGATGAATTTAG
ACGAAGATGAATTTTTAAATGTGAAACGATACTCATTTGATGAAGCGGTCGATTTACTGGACCGAGGCATCATTACCGAC
GCCAAGACAATTATGGCAATCCAATGGTGGCAATTGTCTAGATTGAAATAA

Upstream 100 bases:

>100_bases
TCATCGTTCCGAGTCCGAGTTCACTCATTTGGATTCCTCCTTGTTCGAAATGTAGGTTTGACTCATAATCAGAGTATATC
GAAAAGTGAGGAGTAATCAT

Downstream 100 bases:

>100_bases
TTCTAATCTAGGTACTTATATGATAAACTTACATAGTAATGATTTTAAATTGAAAATTGAGTAAAACTTCGGTATGCTTT
CAGTAGTGAACTATGTAAAG

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MEEKTIEREVIYQGKIFDVEKHVVSLPNGNTSVRELVYHNGAVAVLVIDESDNIVMVEQYRKAFESMSLEIPAGKLEKGE
EPLASAERELEEETGYTAESLEKIFSFYGAPGFCSERVDVYVAKGLSAGEMNLDEDEFLNVKRYSFDEAVDLLDRGIITD
AKTIMAIQWWQLSRLK

Sequences:

>Translated_176_residues
MEEKTIEREVIYQGKIFDVEKHVVSLPNGNTSVRELVYHNGAVAVLVIDESDNIVMVEQYRKAFESMSLEIPAGKLEKGE
EPLASAERELEEETGYTAESLEKIFSFYGAPGFCSERVDVYVAKGLSAGEMNLDEDEFLNVKRYSFDEAVDLLDRGIITD
AKTIMAIQWWQLSRLK
>Mature_176_residues
MEEKTIEREVIYQGKIFDVEKHVVSLPNGNTSVRELVYHNGAVAVLVIDESDNIVMVEQYRKAFESMSLEIPAGKLEKGE
EPLASAERELEEETGYTAESLEKIFSFYGAPGFCSERVDVYVAKGLSAGEMNLDEDEFLNVKRYSFDEAVDLLDRGIITD
AKTIMAIQWWQLSRLK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=184, Percent_Identity=33.1521739130435, Blast_Score=72, Evalue=1e-14,
Organism=Escherichia coli, GI1789800, Length=160, Percent_Identity=26.875, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788810, Length=144, Percent_Identity=29.1666666666667, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 19952; Mature: 19952

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKTIEREVIYQGKIFDVEKHVVSLPNGNTSVRELVYHNGAVAVLVIDESDNIVMVEQY
CCCCHHHHHHEECCCEEEEHHHEEECCCCCHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
RKAFESMSLEIPAGKLEKGEEPLASAERELEEETGYTAESLEKIFSFYGAPGFCSERVDV
HHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEE
YVAKGLSAGEMNLDEDEFLNVKRYSFDEAVDLLDRGIITDAKTIMAIQWWQLSRLK
EEECCCCCCCCCCCHHHHCCCHHCCHHHHHHHHHCCCCCCCHHHEEEEHHHHCCCC
>Mature Secondary Structure
MEEKTIEREVIYQGKIFDVEKHVVSLPNGNTSVRELVYHNGAVAVLVIDESDNIVMVEQY
CCCCHHHHHHEECCCEEEEHHHEEECCCCCHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
RKAFESMSLEIPAGKLEKGEEPLASAERELEEETGYTAESLEKIFSFYGAPGFCSERVDV
HHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEE
YVAKGLSAGEMNLDEDEFLNVKRYSFDEAVDLLDRGIITDAKTIMAIQWWQLSRLK
EEECCCCCCCCCCCHHHHCCCHHCCHHHHHHHHHCCCCCCCHHHEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]