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The map label for this gene is punA [H]

Identifier: 229916181

GI number: 229916181

Start: 411876

End: 412685

Strand: Reverse

Name: punA [H]

Synonym: EAT1b_0450

Alternate gene names: 229916181

Gene position: 412685-411876 (Counterclockwise)

Preceding gene: 229916182

Following gene: 229916180

Centisome position: 13.76

GC content: 51.85

Gene sequence:

>810_bases
ATGGTAAATTGGAACGAAACAAAAGCGTTTTTGCAGGAGCGTATGGGCAGTACACCAGAAATCGGACTCATTCTCGGGTC
AGGACTCGGAGTCCTCGCGGACGAGATTGAGAACCCGGTCGCCATTCCATATGAGGAGATCCCGCACTTCCCGGTATCGA
CAGTTGAAGGACATGCGGGGCAACTCGTGTTCGGTGAGCTCGAAGGGAAACGGGTCGTCGCCATGCAAGGACGGTTCCAC
TTCTATGAAGGATACTCGATGGAACAAGTGACGTTCCCTGTACGTGTCCTCAAGCTGATTGGTGTCGAGACTCTCATCGT
CACGAACGCGGCAGGCGCATGTAACGAGTCATTCAACCCAGGCGACTTGATGATCATCACAGATCATATCAACTTCTTCG
GTACGAGCCCGCTCATCGGGAAGAACGCGAGTGAATTCGGAACACGTTTCCCTGACATGTCAGAAGCGTACGATAAAGAG
CTCGTGAAATTGACAGAACGTGTCGCGTCAGACCTTGGACTTTCTGTACAAAAAGGGGTTTACTTCGGAAACACAGGTCC
TACGTACGAAACACCGGCTGAAGTCAAAATGGCCCGTCTTCTCGGGGGAGACGTTGTCGGGATGTCGACTGTTCCAGAAG
TCATCGTCGCGCGTCATTCGGATATGCGCGTCCTCGGGATTTCGTGTGTCTCGAACATGGCAGCAGGCATCTTAGATCAA
CCACTCAACCATGAAGAAGTCATCGAAACGACAGAGCGCGTTCGTCAAGACTTCCTCTCTCTCGTACGTGGCACGATTAA
AAGCATGTAA

Upstream 100 bases:

>100_bases
ACATTCGCGGACATCGGGGCGACGGTCGCTGACAACTTTGACGTCGCGGCACCATCACACGGAAAGAGTTTCTTAAACGA
AATTTGAGGAGGCAATATTC

Downstream 100 bases:

>100_bases
GTAACTTAATTTTAGGAGGACGACAGTTATGCGTATGGTCGATTTGATTTTAAAGAAACGTAACGGTGGAGAATTGAACG
AAGAGGAGATTCGCTTCGTT

Product: purine nucleoside phosphorylase I, inosine and guanosine-specific

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MVNWNETKAFLQERMGSTPEIGLILGSGLGVLADEIENPVAIPYEEIPHFPVSTVEGHAGQLVFGELEGKRVVAMQGRFH
FYEGYSMEQVTFPVRVLKLIGVETLIVTNAAGACNESFNPGDLMIITDHINFFGTSPLIGKNASEFGTRFPDMSEAYDKE
LVKLTERVASDLGLSVQKGVYFGNTGPTYETPAEVKMARLLGGDVVGMSTVPEVIVARHSDMRVLGISCVSNMAAGILDQ
PLNHEEVIETTERVRQDFLSLVRGTIKSM

Sequences:

>Translated_269_residues
MVNWNETKAFLQERMGSTPEIGLILGSGLGVLADEIENPVAIPYEEIPHFPVSTVEGHAGQLVFGELEGKRVVAMQGRFH
FYEGYSMEQVTFPVRVLKLIGVETLIVTNAAGACNESFNPGDLMIITDHINFFGTSPLIGKNASEFGTRFPDMSEAYDKE
LVKLTERVASDLGLSVQKGVYFGNTGPTYETPAEVKMARLLGGDVVGMSTVPEVIVARHSDMRVLGISCVSNMAAGILDQ
PLNHEEVIETTERVRQDFLSLVRGTIKSM
>Mature_269_residues
MVNWNETKAFLQERMGSTPEIGLILGSGLGVLADEIENPVAIPYEEIPHFPVSTVEGHAGQLVFGELEGKRVVAMQGRFH
FYEGYSMEQVTFPVRVLKLIGVETLIVTNAAGACNESFNPGDLMIITDHINFFGTSPLIGKNASEFGTRFPDMSEAYDKE
LVKLTERVASDLGLSVQKGVYFGNTGPTYETPAEVKMARLLGGDVVGMSTVPEVIVARHSDMRVLGISCVSNMAAGILDQ
PLNHEEVIETTERVRQDFLSLVRGTIKSM

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=276, Percent_Identity=46.3768115942029, Blast_Score=238, Evalue=3e-63,
Organism=Escherichia coli, GI1788746, Length=252, Percent_Identity=47.6190476190476, Blast_Score=252, Evalue=2e-68,
Organism=Caenorhabditis elegans, GI17541190, Length=254, Percent_Identity=41.7322834645669, Blast_Score=203, Evalue=7e-53,
Organism=Saccharomyces cerevisiae, GI6323238, Length=267, Percent_Identity=45.6928838951311, Blast_Score=203, Evalue=3e-53,
Organism=Drosophila melanogaster, GI45552887, Length=273, Percent_Identity=47.985347985348, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45552885, Length=273, Percent_Identity=47.985347985348, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24656093, Length=273, Percent_Identity=47.985347985348, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24656090, Length=273, Percent_Identity=47.985347985348, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24762376, Length=244, Percent_Identity=35.655737704918, Blast_Score=147, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29369; Mature: 29369

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNWNETKAFLQERMGSTPEIGLILGSGLGVLADEIENPVAIPYEEIPHFPVSTVEGHAG
CCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCC
QLVFGELEGKRVVAMQGRFHFYEGYSMEQVTFPVRVLKLIGVETLIVTNAAGACNESFNP
CEEEEECCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCC
GDLMIITDHINFFGTSPLIGKNASEFGTRFPDMSEAYDKELVKLTERVASDLGLSVQKGV
CCEEEEEECCCHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCE
YFGNTGPTYETPAEVKMARLLGGDVVGMSTVPEVIVARHSDMRVLGISCVSNMAAGILDQ
EECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
PLNHEEVIETTERVRQDFLSLVRGTIKSM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVNWNETKAFLQERMGSTPEIGLILGSGLGVLADEIENPVAIPYEEIPHFPVSTVEGHAG
CCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCC
QLVFGELEGKRVVAMQGRFHFYEGYSMEQVTFPVRVLKLIGVETLIVTNAAGACNESFNP
CEEEEECCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCHHHEEEECCCCCCCCCCCC
GDLMIITDHINFFGTSPLIGKNASEFGTRFPDMSEAYDKELVKLTERVASDLGLSVQKGV
CCEEEEEECCCHHCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCE
YFGNTGPTYETPAEVKMARLLGGDVVGMSTVPEVIVARHSDMRVLGISCVSNMAAGILDQ
EECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCC
PLNHEEVIETTERVRQDFLSLVRGTIKSM
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9058965 [H]