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The map label for this gene is argH

Identifier: 229916100

GI number: 229916100

Start: 330771

End: 332159

Strand: Direct

Name: argH

Synonym: EAT1b_0369

Alternate gene names: 229916100

Gene position: 330771-332159 (Clockwise)

Preceding gene: 229916099

Following gene: 229916102

Centisome position: 11.03

GC content: 48.38

Gene sequence:

>1389_bases
ATGAGTAAATTATGGGGAGGCCGTTTCGAGAAGACGGCGAGCGATTGGGTCGATGCATTCGGTGCATCGATTGAATTTGA
CGCTCAACTCGTTCTTGAGGATTTAGAGGGTAGCATGGCTCACGTGACGATGTTAGGTGACCAGGGCATCCTCTTAAAGG
AAGAAGTCACTCAAATCCTTGATGGGTTACAACAACTTAAGCAAAAAGCACTCGCGGATGAATTAAAATTCGAGGTGTAT
CACGAAGATATTCATATGAACTTAGAAGCTCTGCTTCATGAAGCGATTGGACCGGTCGCAGGGAAAATGCATACGGCACG
TAGTCGAAACGATCAAGTCGCGACGGACATGCATCTTTACTTGAAAGAACGTGTGAAAGAAGTGATTCAATTGATTGAAT
CGCTTCAACGCGAACTGCATGCTCACGCTTCAGAAAACGTAGAGACGATCATGCCTGGCTATACGCACCTCCAGCGTGCA
CAACCGATTTCACTGGCCCATCATTTGCTCGCCTATTTCTGGATGCTGGAGCGGGACAAGGAGCGGTTTACGGACAGCTT
GAAACGGATTGATTGGTCCCCTCTTGGCGCAGGAGCCTTAGCAGGGACAACGTTTCCGATCGACCGTTCCCGTTCAGCTG
AATTACTTGGCTTCAATCGGGTTTACCCGAACAGCCTCGATGCCGTTAGCGACCGTGACTTCATTCTCGAGTTTCTTTCA
AATGCATCGATGCTCATGATGCACCTCTCCCGCTTCTGTGAAGAATTGATTCTGTGGGCAAGCGAAGAATATCGCTTCAT
CACGGTGTCCGATACGTTCTCTACCGGTTCGAGCATGATGCCACAAAAGAAAAACCCTGACATGGCCGAACTCGTCCGTG
GAAAGTCGGGTCGAGTGATTGGAAACTTGATGGGCTTGTTGACGCTCATGAAAGGACTTCCGCTCGCTTACAACAAAGAT
TTACAGGAAGATAAAGAAGGAATGTTTGACACGGTCAAGACCGTTCAAGGCTCACTCAGTATCATGGCGGGGATGATGAA
TGAATTGACGTTCCACCCGGACGTCATGAAAAAAGCGACCGAGCGTGATTTTTCTAATGCGACCGAACTCGCCGATTATT
TGACGGCAAAAGGGTTACCGTTCCGTGAGGCACACGAGGTGACTGGTAGACTCGTCAAATATTGCGTGGATGAGCGATTG
TATTTACGCGAGCTTCCAATAACGGTGTTCCAAACGTACTCTGAATTGATTCAAGAGGATGTGTATCATGCCTTGACCCC
TGAACAGGCAGTCGCACGTCGAAACAGTTACGGTGGCACTGGATTCGATGCCGTCCGTGAACAATTGAAAGAAGCCTCAG
TATGCATGAGCACGATGACCTATTCTTAA

Upstream 100 bases:

>100_bases
GATACGTTCGATCAGCAAGCAGCTGTCGGATTCATCAAGTTGTGGGGTTTGCCAACAAAAGTTCAATCAGAGGTGTTGAT
GGAAAAGGGAGCGTTCGTCA

Downstream 100 bases:

>100_bases
TCGTCAAGCACCCGTCACGTTACTCTGTGACGGGTCTTTTTATTTATCCTGTTCATTCAAACGTTCTACGAAAGAAGGAA
GTCCTTTCTCAAGTAGGAGA

Product: argininosuccinate lyase

Products: NA

Alternate protein names: ASAL; Arginosuccinase

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSKLWGGRFEKTASDWVDAFGASIEFDAQLVLEDLEGSMAHVTMLGDQGILLKEEVTQILDGLQQLKQKALADELKFEVY
HEDIHMNLEALLHEAIGPVAGKMHTARSRNDQVATDMHLYLKERVKEVIQLIESLQRELHAHASENVETIMPGYTHLQRA
QPISLAHHLLAYFWMLERDKERFTDSLKRIDWSPLGAGALAGTTFPIDRSRSAELLGFNRVYPNSLDAVSDRDFILEFLS
NASMLMMHLSRFCEELILWASEEYRFITVSDTFSTGSSMMPQKKNPDMAELVRGKSGRVIGNLMGLLTLMKGLPLAYNKD
LQEDKEGMFDTVKTVQGSLSIMAGMMNELTFHPDVMKKATERDFSNATELADYLTAKGLPFREAHEVTGRLVKYCVDERL
YLRELPITVFQTYSELIQEDVYHALTPEQAVARRNSYGGTGFDAVREQLKEASVCMSTMTYS

Sequences:

>Translated_462_residues
MSKLWGGRFEKTASDWVDAFGASIEFDAQLVLEDLEGSMAHVTMLGDQGILLKEEVTQILDGLQQLKQKALADELKFEVY
HEDIHMNLEALLHEAIGPVAGKMHTARSRNDQVATDMHLYLKERVKEVIQLIESLQRELHAHASENVETIMPGYTHLQRA
QPISLAHHLLAYFWMLERDKERFTDSLKRIDWSPLGAGALAGTTFPIDRSRSAELLGFNRVYPNSLDAVSDRDFILEFLS
NASMLMMHLSRFCEELILWASEEYRFITVSDTFSTGSSMMPQKKNPDMAELVRGKSGRVIGNLMGLLTLMKGLPLAYNKD
LQEDKEGMFDTVKTVQGSLSIMAGMMNELTFHPDVMKKATERDFSNATELADYLTAKGLPFREAHEVTGRLVKYCVDERL
YLRELPITVFQTYSELIQEDVYHALTPEQAVARRNSYGGTGFDAVREQLKEASVCMSTMTYS
>Mature_461_residues
SKLWGGRFEKTASDWVDAFGASIEFDAQLVLEDLEGSMAHVTMLGDQGILLKEEVTQILDGLQQLKQKALADELKFEVYH
EDIHMNLEALLHEAIGPVAGKMHTARSRNDQVATDMHLYLKERVKEVIQLIESLQRELHAHASENVETIMPGYTHLQRAQ
PISLAHHLLAYFWMLERDKERFTDSLKRIDWSPLGAGALAGTTFPIDRSRSAELLGFNRVYPNSLDAVSDRDFILEFLSN
ASMLMMHLSRFCEELILWASEEYRFITVSDTFSTGSSMMPQKKNPDMAELVRGKSGRVIGNLMGLLTLMKGLPLAYNKDL
QEDKEGMFDTVKTVQGSLSIMAGMMNELTFHPDVMKKATERDFSNATELADYLTAKGLPFREAHEVTGRLVKYCVDERLY
LRELPITVFQTYSELIQEDVYHALTPEQAVARRNSYGGTGFDAVREQLKEASVCMSTMTYS

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI31541964, Length=449, Percent_Identity=42.7616926503341, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI68303542, Length=449, Percent_Identity=42.7616926503341, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI68303549, Length=449, Percent_Identity=40.5345211581292, Blast_Score=325, Evalue=5e-89,
Organism=Homo sapiens, GI68303547, Length=449, Percent_Identity=39.8663697104677, Blast_Score=319, Evalue=4e-87,
Organism=Escherichia coli, GI1790398, Length=438, Percent_Identity=46.5753424657534, Blast_Score=394, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321806, Length=452, Percent_Identity=44.2477876106195, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI221473854, Length=453, Percent_Identity=36.8653421633554, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI78706858, Length=453, Percent_Identity=36.8653421633554, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ARLY_EXISA (C4L2P3)

Other databases:

- EMBL:   CP001615
- RefSeq:   YP_002884746.1
- ProteinModelPortal:   C4L2P3
- GeneID:   7868484
- GenomeReviews:   CP001615_GR
- KEGG:   eat:EAT1b_0369
- OMA:   MAEDLIF
- GO:   GO:0005737
- HAMAP:   MF_00006
- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- PANTHER:   PTHR11444:SF3
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00838

Pfam domain/function: PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.1

Molecular weight: Translated: 52314; Mature: 52182

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLWGGRFEKTASDWVDAFGASIEFDAQLVLEDLEGSMAHVTMLGDQGILLKEEVTQIL
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
DGLQQLKQKALADELKFEVYHEDIHMNLEALLHEAIGPVAGKMHTARSRNDQVATDMHLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKERVKEVIQLIESLQRELHAHASENVETIMPGYTHLQRAQPISLAHHLLAYFWMLERDK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERFTDSLKRIDWSPLGAGALAGTTFPIDRSRSAELLGFNRVYPNSLDAVSDRDFILEFLS
HHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHC
NASMLMMHLSRFCEELILWASEEYRFITVSDTFSTGSSMMPQKKNPDMAELVRGKSGRVI
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCHH
GNLMGLLTLMKGLPLAYNKDLQEDKEGMFDTVKTVQGSLSIMAGMMNELTFHPDVMKKAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ERDFSNATELADYLTAKGLPFREAHEVTGRLVKYCVDERLYLRELPITVFQTYSELIQED
HHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYHALTPEQAVARRNSYGGTGFDAVREQLKEASVCMSTMTYS
HHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKLWGGRFEKTASDWVDAFGASIEFDAQLVLEDLEGSMAHVTMLGDQGILLKEEVTQIL
CCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHH
DGLQQLKQKALADELKFEVYHEDIHMNLEALLHEAIGPVAGKMHTARSRNDQVATDMHLY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LKERVKEVIQLIESLQRELHAHASENVETIMPGYTHLQRAQPISLAHHLLAYFWMLERDK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
ERFTDSLKRIDWSPLGAGALAGTTFPIDRSRSAELLGFNRVYPNSLDAVSDRDFILEFLS
HHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCHHEECCCCCCCCCCCCCCHHHHHHHHC
NASMLMMHLSRFCEELILWASEEYRFITVSDTFSTGSSMMPQKKNPDMAELVRGKSGRVI
CHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCHH
GNLMGLLTLMKGLPLAYNKDLQEDKEGMFDTVKTVQGSLSIMAGMMNELTFHPDVMKKAT
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ERDFSNATELADYLTAKGLPFREAHEVTGRLVKYCVDERLYLRELPITVFQTYSELIQED
HHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VYHALTPEQAVARRNSYGGTGFDAVREQLKEASVCMSTMTYS
HHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA