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The map label for this gene is argG [H]

Identifier: 229916099

GI number: 229916099

Start: 329567

End: 330778

Strand: Direct

Name: argG [H]

Synonym: EAT1b_0368

Alternate gene names: 229916099

Gene position: 329567-330778 (Clockwise)

Preceding gene: 229916098

Following gene: 229916100

Centisome position: 10.99

GC content: 48.43

Gene sequence:

>1212_bases
TTGAAACAAAAACTCGTATTGGCTTACTCAGGTGGTCTTGACACATCGGTTGCGATTAAATGGTTGGACGAACAAGGTTA
TGATGTGATTGCAGTCTGTTTGAACGTCGGGGAAGGGAAAGACTTAGAAAAGATTCAACAAAAAGCGATGAAAGTTGGAG
CGAAAAAATCAATCGTCATCGATGCGGTCGATGAGTTCGTCAACGAGTTCGCTCGCTATTCGATGCAGGCTCATACATTA
TATGAAGGCATATATCCACTCGTTTCGGCACTATCACGCCCCCTCATCTCGAAAAAATTGGTCGAGGTCGCACAGGCCGA
AGGCGCCGTCGCTGTGGCTCATGGATGTACCGGGAAAGGAAATGACCAAGTGCGTTTTGAAGTGTCCATTCATGCACTCG
ATCCTTCACTCGAAATCGTCGCACCGGTTCGGGAATGGAAATGGTCACGTGAGGAAGAAATCGCGTATGCGGCGAAACAT
CAAATTCCGATTCCAATCGTTCAAGAAGAGCCATTCTCCATCGATCAGAACATCTGGGGACGCGCCATCGAATGCGGCAT
CTTAGAAGATCCTTGGGCAGCACCTCCGGCAAGCGCATATGAGTTAACAGCGGCACTTGAAGACACACCGCATGAACCGA
CTTATCTTGAGATTGAATTCAAGAGCGGAGTGCCGGTTGCTATTGACGGGAAGAGCCAATCGTTCACCGATATCTTAAAA
GAGCTCAATATCGTCGCGGGTGCCCACGGCGTCGGGAAGATCGACCACATCGAGAACCGGGTCGTCGGTATCAAATCACG
CGAGGTGTATGAGGCTCCTGGTGCCATGACATTGATCACTGCACATAAAGCTCTTGAAGCCTTGACACTCGTTCGTGAAG
TGGCGCATTTCAAACCGATTGTCGAACAAAAATTGACGGAAACGATCTACAACGGACTTTGGTATTCACAGCTCACAAAA
GCACTTCTTGCGTTTATCGATGAGACTCAAGCGACCGTCACAGGAACGGTTCGCATGAAACTGTATAAAGGACAAGCCAT
CGTCGACGGACGGAAGTCGCCAATTTCTCTTTATGATGAGGAACTTGCGACCTATACGTCAGCCGATACGTTCGATCAGC
AAGCAGCTGTCGGATTCATCAAGTTGTGGGGTTTGCCAACAAAAGTTCAATCAGAGGTGTTGATGGAAAAGGGAGCGTTC
GTCAATGAGTAA

Upstream 100 bases:

>100_bases
CATTTTCAAAAAAGGGTTGCCACACGACATCTTATCCCTTACACTGAGTTGTATATTTATAAATAAATATTAATAATTAT
TCATAAGGGGTGTTCATATT

Downstream 100 bases:

>100_bases
ATTATGGGGAGGCCGTTTCGAGAAGACGGCGAGCGATTGGGTCGATGCATTCGGTGCATCGATTGAATTTGACGCTCAAC
TCGTTCTTGAGGATTTAGAG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKQKLVLAYSGGLDTSVAIKWLDEQGYDVIAVCLNVGEGKDLEKIQQKAMKVGAKKSIVIDAVDEFVNEFARYSMQAHTL
YEGIYPLVSALSRPLISKKLVEVAQAEGAVAVAHGCTGKGNDQVRFEVSIHALDPSLEIVAPVREWKWSREEEIAYAAKH
QIPIPIVQEEPFSIDQNIWGRAIECGILEDPWAAPPASAYELTAALEDTPHEPTYLEIEFKSGVPVAIDGKSQSFTDILK
ELNIVAGAHGVGKIDHIENRVVGIKSREVYEAPGAMTLITAHKALEALTLVREVAHFKPIVEQKLTETIYNGLWYSQLTK
ALLAFIDETQATVTGTVRMKLYKGQAIVDGRKSPISLYDEELATYTSADTFDQQAAVGFIKLWGLPTKVQSEVLMEKGAF
VNE

Sequences:

>Translated_403_residues
MKQKLVLAYSGGLDTSVAIKWLDEQGYDVIAVCLNVGEGKDLEKIQQKAMKVGAKKSIVIDAVDEFVNEFARYSMQAHTL
YEGIYPLVSALSRPLISKKLVEVAQAEGAVAVAHGCTGKGNDQVRFEVSIHALDPSLEIVAPVREWKWSREEEIAYAAKH
QIPIPIVQEEPFSIDQNIWGRAIECGILEDPWAAPPASAYELTAALEDTPHEPTYLEIEFKSGVPVAIDGKSQSFTDILK
ELNIVAGAHGVGKIDHIENRVVGIKSREVYEAPGAMTLITAHKALEALTLVREVAHFKPIVEQKLTETIYNGLWYSQLTK
ALLAFIDETQATVTGTVRMKLYKGQAIVDGRKSPISLYDEELATYTSADTFDQQAAVGFIKLWGLPTKVQSEVLMEKGAF
VNE
>Mature_403_residues
MKQKLVLAYSGGLDTSVAIKWLDEQGYDVIAVCLNVGEGKDLEKIQQKAMKVGAKKSIVIDAVDEFVNEFARYSMQAHTL
YEGIYPLVSALSRPLISKKLVEVAQAEGAVAVAHGCTGKGNDQVRFEVSIHALDPSLEIVAPVREWKWSREEEIAYAAKH
QIPIPIVQEEPFSIDQNIWGRAIECGILEDPWAAPPASAYELTAALEDTPHEPTYLEIEFKSGVPVAIDGKSQSFTDILK
ELNIVAGAHGVGKIDHIENRVVGIKSREVYEAPGAMTLITAHKALEALTLVREVAHFKPIVEQKLTETIYNGLWYSQLTK
ALLAFIDETQATVTGTVRMKLYKGQAIVDGRKSPISLYDEELATYTSADTFDQQAAVGFIKLWGLPTKVQSEVLMEKGAF
VNE

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=397, Percent_Identity=43.8287153652393, Blast_Score=307, Evalue=1e-83,
Organism=Homo sapiens, GI16950633, Length=397, Percent_Identity=43.8287153652393, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1789563, Length=370, Percent_Identity=29.1891891891892, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324514, Length=413, Percent_Identity=37.772397094431, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI21358151, Length=395, Percent_Identity=39.4936708860759, Blast_Score=273, Evalue=1e-73,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44369; Mature: 44369

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKLVLAYSGGLDTSVAIKWLDEQGYDVIAVCLNVGEGKDLEKIQQKAMKVGAKKSIVI
CCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEH
DAVDEFVNEFARYSMQAHTLYEGIYPLVSALSRPLISKKLVEVAQAEGAVAVAHGCTGKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NDQVRFEVSIHALDPSLEIVAPVREWKWSREEEIAYAAKHQIPIPIVQEEPFSIDQNIWG
CCEEEEEEEEEEECCCCEEEECHHHCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
RAIECGILEDPWAAPPASAYELTAALEDTPHEPTYLEIEFKSGVPVAIDGKSQSFTDILK
CEEECCCCCCCCCCCCCCHHEEEEHHCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHH
ELNIVAGAHGVGKIDHIENRVVGIKSREVYEAPGAMTLITAHKALEALTLVREVAHFKPI
HHHHHHCCCCCCCHHHHCCCEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
VEQKLTETIYNGLWYSQLTKALLAFIDETQATVTGTVRMKLYKGQAIVDGRKSPISLYDE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEECCCCCCCHHHHH
ELATYTSADTFDQQAAVGFIKLWGLPTKVQSEVLMEKGAFVNE
HHHHHCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKQKLVLAYSGGLDTSVAIKWLDEQGYDVIAVCLNVGEGKDLEKIQQKAMKVGAKKSIVI
CCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEH
DAVDEFVNEFARYSMQAHTLYEGIYPLVSALSRPLISKKLVEVAQAEGAVAVAHGCTGKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
NDQVRFEVSIHALDPSLEIVAPVREWKWSREEEIAYAAKHQIPIPIVQEEPFSIDQNIWG
CCEEEEEEEEEEECCCCEEEECHHHCCCCCHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
RAIECGILEDPWAAPPASAYELTAALEDTPHEPTYLEIEFKSGVPVAIDGKSQSFTDILK
CEEECCCCCCCCCCCCCCHHEEEEHHCCCCCCCCEEEEEECCCCCEEECCCCCHHHHHHH
ELNIVAGAHGVGKIDHIENRVVGIKSREVYEAPGAMTLITAHKALEALTLVREVAHFKPI
HHHHHHCCCCCCCHHHHCCCEEECCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
VEQKLTETIYNGLWYSQLTKALLAFIDETQATVTGTVRMKLYKGQAIVDGRKSPISLYDE
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCEEECCCCCCCHHHHH
ELATYTSADTFDQQAAVGFIKLWGLPTKVQSEVLMEKGAFVNE
HHHHHCCCCCCCHHHHHHHHEECCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA