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The map label for this gene is hetM [H]

Identifier: 229916033

GI number: 229916033

Start: 270510

End: 271547

Strand: Direct

Name: hetM [H]

Synonym: EAT1b_0301

Alternate gene names: 229916033

Gene position: 270510-271547 (Clockwise)

Preceding gene: 229916031

Following gene: 229916036

Centisome position: 9.02

GC content: 48.55

Gene sequence:

>1038_bases
TTGTATATTTTTATGACTGGTGGAACAGGATTTTTAGGAGGGCGACTCGCCAAAGAATTGATTCAGCGGGGGCATCGCGT
CTGCATGTTGGCTCGAACACCGGAGAAGGTACAGACACAATTCACCGCAGAAGAACAGAAGTCGATTGATGTGTTACCAG
GCGATTTGACGTCACACGCACTCGGTGCGAATGAAGCGTTCATCGAGACACATAGAGGGAAGATTGATTTATTCCTACAC
ATGGCAGCACTCGTCAAATTCGATGAAGAATTGCGCGATGAATTGTTCGAGACCAATTTGACCGGGACGAAAGAGGCCCT
TCGCTTGACCAAGCTGATTGACTGCCGTCGCTTTTTCCACGTGAGTACGGCCTATACACTCGGCGCTAAGGAAGTTGGCG
GGGAAACACTGTACGACGTGTCGCAATCTTTTTTGAATCCGTATGAAGAAAGCAAGGCACACGCCGAGCAGGCGGTCTGG
GAGTCACGGGATGAGCTTGATGTGTCAATCTTCCGACCGGCCATCATCGTCGGAGACTCGAAAACAGGAGAGGCTGACTC
AAAATTTACGATGTACGGATATATGCGTGCTCTCGAACTGTTCAAACGGAAATTGGAACGACGTGGGCATGACTTCGAGT
CGATTCGATTGATTGGCTCAGCGACAGGTACATCAAACCTTGTCCCTGTCGATTATGTCGCGGATGTACTCCTCGCTGCC
ATCGAGCATGCGAAGGCAGGTACAATCTATAACGTGACAAACGACGCACCGCCAAAAAATGAAACGATGCTCGACTATAT
GAAAGAGTCGTTAGCATTCCCGCATTTAACAATCACGGAGACACCGACATCGACACTCAGCTCAATCGAACAGTCGTTCA
ACGATATGGTGAAAGTGTTCAATCCGTATTTGAACCGGGATATCACGTTCCGTGACGACAACACGAAGCAATTGCTACAG
GATGCTGGATTCGCTCCGCTCCATCTCGATCAAAAATCGTTACGTCGCATCGTGAATGCCTATTACAAAGCAAAATAA

Upstream 100 bases:

>100_bases
CGATTTTTTCTAAACATGGAATTTTACGAGAGGCAAAGTGGCTACATGCGACAAGAGTTGATATGATGGGTAAAGAATGT
CACAGAGAGGGAGAACGCGA

Downstream 100 bases:

>100_bases
AAAATGGACCGATTTTTCCGGTCCATTTTTTATTTATCACAAACTAACACGATTTTACCATCTTTGATTTCACGTTCGAG
TGAATCCACCATCTCTTTTG

Product: Male sterility domain protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MYIFMTGGTGFLGGRLAKELIQRGHRVCMLARTPEKVQTQFTAEEQKSIDVLPGDLTSHALGANEAFIETHRGKIDLFLH
MAALVKFDEELRDELFETNLTGTKEALRLTKLIDCRRFFHVSTAYTLGAKEVGGETLYDVSQSFLNPYEESKAHAEQAVW
ESRDELDVSIFRPAIIVGDSKTGEADSKFTMYGYMRALELFKRKLERRGHDFESIRLIGSATGTSNLVPVDYVADVLLAA
IEHAKAGTIYNVTNDAPPKNETMLDYMKESLAFPHLTITETPTSTLSSIEQSFNDMVKVFNPYLNRDITFRDDNTKQLLQ
DAGFAPLHLDQKSLRRIVNAYYKAK

Sequences:

>Translated_345_residues
MYIFMTGGTGFLGGRLAKELIQRGHRVCMLARTPEKVQTQFTAEEQKSIDVLPGDLTSHALGANEAFIETHRGKIDLFLH
MAALVKFDEELRDELFETNLTGTKEALRLTKLIDCRRFFHVSTAYTLGAKEVGGETLYDVSQSFLNPYEESKAHAEQAVW
ESRDELDVSIFRPAIIVGDSKTGEADSKFTMYGYMRALELFKRKLERRGHDFESIRLIGSATGTSNLVPVDYVADVLLAA
IEHAKAGTIYNVTNDAPPKNETMLDYMKESLAFPHLTITETPTSTLSSIEQSFNDMVKVFNPYLNRDITFRDDNTKQLLQ
DAGFAPLHLDQKSLRRIVNAYYKAK
>Mature_345_residues
MYIFMTGGTGFLGGRLAKELIQRGHRVCMLARTPEKVQTQFTAEEQKSIDVLPGDLTSHALGANEAFIETHRGKIDLFLH
MAALVKFDEELRDELFETNLTGTKEALRLTKLIDCRRFFHVSTAYTLGAKEVGGETLYDVSQSFLNPYEESKAHAEQAVW
ESRDELDVSIFRPAIIVGDSKTGEADSKFTMYGYMRALELFKRKLERRGHDFESIRLIGSATGTSNLVPVDYVADVLLAA
IEHAKAGTIYNVTNDAPPKNETMLDYMKESLAFPHLTITETPTSTLSSIEQSFNDMVKVFNPYLNRDITFRDDNTKQLLQ
DAGFAPLHLDQKSLRRIVNAYYKAK

Specific function: Galactose metabolism; third step. [C]

COG id: COG3320

COG function: function code Q; Putative dehydrogenase domain of multifunctional non-ribosomal peptide synthetases and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572029, Length=351, Percent_Identity=24.7863247863248, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009081
- InterPro:   IPR013120
- InterPro:   IPR016040
- InterPro:   IPR006163
- InterPro:   IPR010080 [H]

Pfam domain/function: PF07993 NAD_binding_4; PF00550 PP-binding [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 38910; Mature: 38910

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIFMTGGTGFLGGRLAKELIQRGHRVCMLARTPEKVQTQFTAEEQKSIDVLPGDLTSHA
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LGANEAFIETHRGKIDLFLHMAALVKFDEELRDELFETNLTGTKEALRLTKLIDCRRFFH
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VSTAYTLGAKEVGGETLYDVSQSFLNPYEESKAHAEQAVWESRDELDVSIFRPAIIVGDS
HHHHHCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCC
KTGEADSKFTMYGYMRALELFKRKLERRGHDFESIRLIGSATGTSNLVPVDYVADVLLAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHH
IEHAKAGTIYNVTNDAPPKNETMLDYMKESLAFPHLTITETPTSTLSSIEQSFNDMVKVF
HHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
NPYLNRDITFRDDNTKQLLQDAGFAPLHLDQKSLRRIVNAYYKAK
HHHHCCCEEECCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYIFMTGGTGFLGGRLAKELIQRGHRVCMLARTPEKVQTQFTAEEQKSIDVLPGDLTSHA
CEEEEECCCCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHH
LGANEAFIETHRGKIDLFLHMAALVKFDEELRDELFETNLTGTKEALRLTKLIDCRRFFH
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
VSTAYTLGAKEVGGETLYDVSQSFLNPYEESKAHAEQAVWESRDELDVSIFRPAIIVGDS
HHHHHCCCHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEECCC
KTGEADSKFTMYGYMRALELFKRKLERRGHDFESIRLIGSATGTSNLVPVDYVADVLLAA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCHHHHHHHHHHH
IEHAKAGTIYNVTNDAPPKNETMLDYMKESLAFPHLTITETPTSTLSSIEQSFNDMVKVF
HHHHCCCCEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHH
NPYLNRDITFRDDNTKQLLQDAGFAPLHLDQKSLRRIVNAYYKAK
HHHHCCCEEECCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]