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The map label for this gene is glnA [H]

Identifier: 229916032

GI number: 229916032

Start: 269025

End: 270320

Strand: Reverse

Name: glnA [H]

Synonym: EAT1b_0300

Alternate gene names: 229916032

Gene position: 270320-269025 (Counterclockwise)

Preceding gene: 229916034

Following gene: 229916028

Centisome position: 9.01

GC content: 47.84

Gene sequence:

>1296_bases
ATGGCAAATCTATTCGGAACAGACAAGGATGACGTAAAAAAGAAGCAACAAGATATTGAGAAAACGGTACAAGAGAAAAA
CATCACCCAAATCCATCTATATTTCTCAGATGTTCTCGGTGATTTAAAGCTCTTGACGCTTTCGGCAAAACTGTTGCCAG
AAGTCTTTGAAGGAAAAACGATGTTTGACGGTTCTTCGATCAACGGGTTCTCTTCCATCAAAAACTCCGACTTATTCTTG
AGACCGGATTTAGATCGTCCACGGTATGAGCAAGACGGCGAGGAAAATATTTTGGCATTCTTCTGTGATGTATGTACTCC
GGAGGGAGAGCGATATGAATACGACCCACGTAATGTATTGAAGGATGCACTCGAACGTGCAGCACGTGATGGCTACTCGG
TATTCGTCGGGGCAGAGCCAGAATTCTTCATCTTTGACGAGAACGGGGAGTTTTATGACGAGGCCGGATATTTCTCGACA
CGTTCAGAGGATAAAGGATATGAAGTGCGACAGCACATCGCGAATCGTCTCAGCGGTGTCGGGTTCAACATTGAGGCAGT
GCACCATGAGGTCGCCCCTGCGCAACATGAGATCGGCATCAAGTATGATGACGCACTCCATACAGCGGATAACATTCAGT
TCTTCAAAGAGATTGTCAAGATTGCGGCAAAAGAGAAAGGGGCATCCGTCTCATTCTTACCGAAACCGTTGCGTGATGTG
AACGGTTCTGGCATGCACTTGAACATCTCTGTCTGGAAGAATGATGAGTCAGGAAATCCGACGACCAACGCGTTCGACGA
AGTCGATTCGGATTACGGATTGTCGAAGACAGCACGTCATTTCATCGAGGGAATCTTGAAGCATGCCCGTGCCACGGCAA
TCTTCACGAATCCGCTACCTGAATCATACGAGCGTCTCGTTCCTGGATTCGAGGCACCCGTCAACATTTGCTGGAGCCCG
TCGAACCGTAGTTCGATGATTCGAATCCCGGCTTCTCGTGGGAGTGCGACCCGTGTCGAGGTTCGGAACCCTGACCCGAG
TGCGAACCCATATTTGGCACTTGCTGCGCTCATCTATGCCGGACTTGAAGGAATCGAACAACAACTCGTCCCGTCTTCAC
CGAATGAGGATGACTTGTTCGAGTATTCAGCGAAGCAAATCGAGAGCCAAGGAATCGCATCACTGCCTGCGACATTCCAT
GAATCGATCCAATCACTGAAACAAGACAACTTCCATGACTACTTTAACCACAATATGATCGATCGCTACTTGTCACTCAT
TCAAGTGCGAAAATAA

Upstream 100 bases:

>100_bases
AGAAAAAATCGAACTCGGTTATGAACTATAGAGAAGCGTACGAACGGTGCGTAACGACAGCTTGCCTGTCAAATCCATTG
AAGTGAGGTTTTAACGAATC

Downstream 100 bases:

>100_bases
AAATGACGCCAGCCGATTCAATCGGCTGGCGTTTTTGATGATTAATGGATTCCACGCATGTACACTTTGATTTTTGGTGC
GAGTGCGAACAGAATCAAAC

Product: glutamine synthetase catalytic region

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MANLFGTDKDDVKKKQQDIEKTVQEKNITQIHLYFSDVLGDLKLLTLSAKLLPEVFEGKTMFDGSSINGFSSIKNSDLFL
RPDLDRPRYEQDGEENILAFFCDVCTPEGERYEYDPRNVLKDALERAARDGYSVFVGAEPEFFIFDENGEFYDEAGYFST
RSEDKGYEVRQHIANRLSGVGFNIEAVHHEVAPAQHEIGIKYDDALHTADNIQFFKEIVKIAAKEKGASVSFLPKPLRDV
NGSGMHLNISVWKNDESGNPTTNAFDEVDSDYGLSKTARHFIEGILKHARATAIFTNPLPESYERLVPGFEAPVNICWSP
SNRSSMIRIPASRGSATRVEVRNPDPSANPYLALAALIYAGLEGIEQQLVPSSPNEDDLFEYSAKQIESQGIASLPATFH
ESIQSLKQDNFHDYFNHNMIDRYLSLIQVRK

Sequences:

>Translated_431_residues
MANLFGTDKDDVKKKQQDIEKTVQEKNITQIHLYFSDVLGDLKLLTLSAKLLPEVFEGKTMFDGSSINGFSSIKNSDLFL
RPDLDRPRYEQDGEENILAFFCDVCTPEGERYEYDPRNVLKDALERAARDGYSVFVGAEPEFFIFDENGEFYDEAGYFST
RSEDKGYEVRQHIANRLSGVGFNIEAVHHEVAPAQHEIGIKYDDALHTADNIQFFKEIVKIAAKEKGASVSFLPKPLRDV
NGSGMHLNISVWKNDESGNPTTNAFDEVDSDYGLSKTARHFIEGILKHARATAIFTNPLPESYERLVPGFEAPVNICWSP
SNRSSMIRIPASRGSATRVEVRNPDPSANPYLALAALIYAGLEGIEQQLVPSSPNEDDLFEYSAKQIESQGIASLPATFH
ESIQSLKQDNFHDYFNHNMIDRYLSLIQVRK
>Mature_430_residues
ANLFGTDKDDVKKKQQDIEKTVQEKNITQIHLYFSDVLGDLKLLTLSAKLLPEVFEGKTMFDGSSINGFSSIKNSDLFLR
PDLDRPRYEQDGEENILAFFCDVCTPEGERYEYDPRNVLKDALERAARDGYSVFVGAEPEFFIFDENGEFYDEAGYFSTR
SEDKGYEVRQHIANRLSGVGFNIEAVHHEVAPAQHEIGIKYDDALHTADNIQFFKEIVKIAAKEKGASVSFLPKPLRDVN
GSGMHLNISVWKNDESGNPTTNAFDEVDSDYGLSKTARHFIEGILKHARATAIFTNPLPESYERLVPGFEAPVNICWSPS
NRSSMIRIPASRGSATRVEVRNPDPSANPYLALAALIYAGLEGIEQQLVPSSPNEDDLFEYSAKQIESQGIASLPATFHE
SIQSLKQDNFHDYFNHNMIDRYLSLIQVRK

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=343, Percent_Identity=25.0728862973761, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI1790301, Length=446, Percent_Identity=33.6322869955157, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87081870, Length=281, Percent_Identity=26.3345195729537, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 48526; Mature: 48394

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLFGTDKDDVKKKQQDIEKTVQEKNITQIHLYFSDVLGDLKLLTLSAKLLPEVFEGKT
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCE
MFDGSSINGFSSIKNSDLFLRPDLDRPRYEQDGEENILAFFCDVCTPEGERYEYDPRNVL
EECCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHEEHHCCCCCCCEECCHHHHH
KDALERAARDGYSVFVGAEPEFFIFDENGEFYDEAGYFSTRSEDKGYEVRQHIANRLSGV
HHHHHHHHCCCCEEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
GFNIEAVHHEVAPAQHEIGIKYDDALHTADNIQFFKEIVKIAAKEKGASVSFLPKPLRDV
CCCHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
NGSGMHLNISVWKNDESGNPTTNAFDEVDSDYGLSKTARHFIEGILKHARATAIFTNPLP
CCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCC
ESYERLVPGFEAPVNICWSPSNRSSMIRIPASRGSATRVEVRNPDPSANPYLALAALIYA
HHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHH
GLEGIEQQLVPSSPNEDDLFEYSAKQIESQGIASLPATFHESIQSLKQDNFHDYFNHNMI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCHHHHCHHHH
DRYLSLIQVRK
HHHHHHHHCCC
>Mature Secondary Structure 
ANLFGTDKDDVKKKQQDIEKTVQEKNITQIHLYFSDVLGDLKLLTLSAKLLPEVFEGKT
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHCCCE
MFDGSSINGFSSIKNSDLFLRPDLDRPRYEQDGEENILAFFCDVCTPEGERYEYDPRNVL
EECCCCCCCHHHCCCCCEEEECCCCCCCCCCCCCCCHHHHEEHHCCCCCCCEECCHHHHH
KDALERAARDGYSVFVGAEPEFFIFDENGEFYDEAGYFSTRSEDKGYEVRQHIANRLSGV
HHHHHHHHCCCCEEEECCCCCEEEECCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHCC
GFNIEAVHHEVAPAQHEIGIKYDDALHTADNIQFFKEIVKIAAKEKGASVSFLPKPLRDV
CCCHHHHHHHHCCCHHHCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHCC
NGSGMHLNISVWKNDESGNPTTNAFDEVDSDYGLSKTARHFIEGILKHARATAIFTNPLP
CCCCEEEEEEEEECCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCCC
ESYERLVPGFEAPVNICWSPSNRSSMIRIPASRGSATRVEVRNPDPSANPYLALAALIYA
HHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHH
GLEGIEQQLVPSSPNEDDLFEYSAKQIESQGIASLPATFHESIQSLKQDNFHDYFNHNMI
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHCCCHHHHCHHHH
DRYLSLIQVRK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2906311; 2565853; 9384377; 11719184 [H]