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The map label for this gene is artP [H]

Identifier: 229915827

GI number: 229915827

Start: 71590

End: 72357

Strand: Reverse

Name: artP [H]

Synonym: EAT1b_0094

Alternate gene names: 229915827

Gene position: 72357-71590 (Counterclockwise)

Preceding gene: 229915828

Following gene: 229915826

Centisome position: 2.41

GC content: 44.53

Gene sequence:

>768_bases
ATGAAAAAATGGTTATTAGCATTTACGCTCATGATGAGCGCAGTATTACTCGGTGCATGTAGTCAAGAAACAGAAGGTGA
AGCGACAGAGCAAAAAACGCTCGTCATGGGGACGAGCGCCGATTACTTCCCATATGAATTTGTCGATACGGCAAACGGGG
ATGCGATTGTCGGATTTGATATTGAAATCGCGGAGACGATTACAGAACGTCTCGGTTACGAATTGAAGATTGAAGATATG
GACTTCGGTAGCTTACTCGGAGCCTTGAACAGCGGGCGTGTCGACTTCGTCATGGCCGGGATGACACCAACAGAAGAGCG
TAAAGAGAATGCTGATTTCTCAGACATCTATCTATCCGCGACAAACTTGATCATGACTAAAGACGAGTCACTTCAGGCAA
TCGAAGATTTGTCTGGTAAGAAAATCGGTGTCCAAACGGCTTCGATTCAAGAAAATATCGCTAAAGAACAAGCACCTGAC
GCGGAACTCGTATCCTTGAACAAAATCCCTGAAATCGTTCAAGAGTTGAACACAGGTCGAATCGATGCAATGGTCATCGA
AGACACGGTCGCACAAAAGTATTTAGACCAAGATGACAGCTTCTATACATTCGCTTTGAAAGAAGATGGCGAAAAAGGAT
CAGCTGCTGCCTTCAAATTAGATGATGAGTTACGCGACCAGTTCAACGAAGAGCTGAACAAGATGATGGAGTCTGGCGAA
ATCGATGAATTGGTGAAAAAATGGTTCTCGATGGAGCCTACTGAATGA

Upstream 100 bases:

>100_bases
AGTGTTTCTTTTTTTAATAATTTTGTATATTTATGCTTTACAATTAATAATCATACATATACACTTAGTCTCATCTACAA
CAGGGAGGCATTATACACAT

Downstream 100 bases:

>100_bases
GACAAGTCGAGCTTCCGATTTATCCCGTCGTTTATCCGACGGGACATCGGTTAAACCAAAATGAAAGTCAGTTCCCGTTA
CCTGACCGCATCAAGAATCA

Product: extracellular solute-binding protein family 3

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKKWLLAFTLMMSAVLLGACSQETEGEATEQKTLVMGTSADYFPYEFVDTANGDAIVGFDIEIAETITERLGYELKIEDM
DFGSLLGALNSGRVDFVMAGMTPTEERKENADFSDIYLSATNLIMTKDESLQAIEDLSGKKIGVQTASIQENIAKEQAPD
AELVSLNKIPEIVQELNTGRIDAMVIEDTVAQKYLDQDDSFYTFALKEDGEKGSAAAFKLDDELRDQFNEELNKMMESGE
IDELVKKWFSMEPTE

Sequences:

>Translated_255_residues
MKKWLLAFTLMMSAVLLGACSQETEGEATEQKTLVMGTSADYFPYEFVDTANGDAIVGFDIEIAETITERLGYELKIEDM
DFGSLLGALNSGRVDFVMAGMTPTEERKENADFSDIYLSATNLIMTKDESLQAIEDLSGKKIGVQTASIQENIAKEQAPD
AELVSLNKIPEIVQELNTGRIDAMVIEDTVAQKYLDQDDSFYTFALKEDGEKGSAAAFKLDDELRDQFNEELNKMMESGE
IDELVKKWFSMEPTE
>Mature_255_residues
MKKWLLAFTLMMSAVLLGACSQETEGEATEQKTLVMGTSADYFPYEFVDTANGDAIVGFDIEIAETITERLGYELKIEDM
DFGSLLGALNSGRVDFVMAGMTPTEERKENADFSDIYLSATNLIMTKDESLQAIEDLSGKKIGVQTASIQENIAKEQAPD
AELVSLNKIPEIVQELNTGRIDAMVIEDTVAQKYLDQDDSFYTFALKEDGEKGSAAAFKLDDELRDQFNEELNKMMESGE
IDELVKKWFSMEPTE

Specific function: Part of a binding-protein-dependent transport system for arginine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=227, Percent_Identity=33.0396475770925, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=26.7489711934156, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788648, Length=233, Percent_Identity=27.0386266094421, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787085, Length=254, Percent_Identity=25.1968503937008, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1788228, Length=236, Percent_Identity=27.1186440677966, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788649, Length=261, Percent_Identity=27.2030651340996, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28390; Mature: 28390

Theoretical pI: Translated: 3.88; Mature: 3.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLAFTLMMSAVLLGACSQETEGEATEQKTLVMGTSADYFPYEFVDTANGDAIVGFD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEC
IEIAETITERLGYELKIEDMDFGSLLGALNSGRVDFVMAGMTPTEERKENADFSDIYLSA
HHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCHHHEEEEE
TNLIMTKDESLQAIEDLSGKKIGVQTASIQENIAKEQAPDAELVSLNKIPEIVQELNTGR
EEEEEECCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
IDAMVIEDTVAQKYLDQDDSFYTFALKEDGEKGSAAAFKLDDELRDQFNEELNKMMESGE
EEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
IDELVKKWFSMEPTE
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKWLLAFTLMMSAVLLGACSQETEGEATEQKTLVMGTSADYFPYEFVDTANGDAIVGFD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCEEEEEC
IEIAETITERLGYELKIEDMDFGSLLGALNSGRVDFVMAGMTPTEERKENADFSDIYLSA
HHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCEEEEEECCCCCHHHHCCCCHHHEEEEE
TNLIMTKDESLQAIEDLSGKKIGVQTASIQENIAKEQAPDAELVSLNKIPEIVQELNTGR
EEEEEECCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCC
IDAMVIEDTVAQKYLDQDDSFYTFALKEDGEKGSAAAFKLDDELRDQFNEELNKMMESGE
EEEEEHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCC
IDELVKKWFSMEPTE
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]