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The map label for this gene is rsmE [H]

Identifier: 229595716

GI number: 229595716

Start: 2628991

End: 2629743

Strand: Reverse

Name: rsmE [H]

Synonym: AZC_2304

Alternate gene names: 229595716

Gene position: 2629743-2628991 (Counterclockwise)

Preceding gene: 158423929

Following gene: 158423927

Centisome position: 48.97

GC content: 70.25

Gene sequence:

>753_bases
ATGCCCGATCATGATTTCCGCGCCCAGCGCCTGTTCGTTCGCGAGGATCTTGCTGCGGACACCACCTTCTCGCTGCCGTC
GGAGCAGGCCCATTACGTCGCGAACGTGATTCGCCTGCCCGTGCGCGGCGGCCTGCTGGTCTTCAACGGGCGGGACGGGG
AGTGGCGGGCGACCCGCATCGAGGGCGGCAAGAAAGAGGTACGCCTGTCCATCGGCGGCCAGACCCGACCCCAGCCGGCT
GCCGCCGGCCTCACCTACGCCTTCGCGCCGCTGAAGCACGCCCGCCTCGATTACATGGTCCAGAAGGCCGTGGAGATGGG
CGCCGGCGTGCTGGCACCAGTGCTGACCCAGCACACGCAGATTCCCCGGGTGAACGGCGAGCGCATGGAGGCGAACGCCA
TCGAGGCGGCCGAACAATGCGGCATCCTCACCATCCCGTCGGTGCGCGCCCCGCAGGGGCTTTCCGCCTTTCTCGGCGCC
CGGCCGGCCGACGACATGCTCGTGTTCTGCGACGAGGCCGCGCCCGTCGCCGACCCGCTCGTGGCGCTGCGGCCTGCGGC
GGGCGGGCCCATCACCGTCCTGATCGGTCCGGAAGGCGGCTTCTCCGCGGACGAGCGGACGCTTCTGCATCGGCAGGAGC
GTGTGATCGTCATCGCACTCGGGCCGCGAATCCTGCGCGCCGACACGGCAGGGGTCGCAGCACTCGCGCTCGTTCAGGCG
GTGTGCGGGGACTGGAGACCCACTTCCGGTTGA

Upstream 100 bases:

>100_bases
CTTGCGCGTCTCGACCCGCCGTGGTGAAGGGCGGGACAGATGGCGCGTACCGCGCCGCGCGATCGCTTTGGGCCGTCCTG
CCACGGCCGGGGAGAGGCCG

Downstream 100 bases:

>100_bases
AGTCGAAAAAGTTCCCGCTCACCGTGTGGAGAGCTGGAAATATTTTCGATCCCACGGCAACGCCAGCGTTCACATTGTGT
TTTGAGGCAGAAGATCAGCG

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MPDHDFRAQRLFVREDLAADTTFSLPSEQAHYVANVIRLPVRGGLLVFNGRDGEWRATRIEGGKKEVRLSIGGQTRPQPA
AAGLTYAFAPLKHARLDYMVQKAVEMGAGVLAPVLTQHTQIPRVNGERMEANAIEAAEQCGILTIPSVRAPQGLSAFLGA
RPADDMLVFCDEAAPVADPLVALRPAAGGPITVLIGPEGGFSADERTLLHRQERVIVIALGPRILRADTAGVAALALVQA
VCGDWRPTSG

Sequences:

>Translated_250_residues
MPDHDFRAQRLFVREDLAADTTFSLPSEQAHYVANVIRLPVRGGLLVFNGRDGEWRATRIEGGKKEVRLSIGGQTRPQPA
AAGLTYAFAPLKHARLDYMVQKAVEMGAGVLAPVLTQHTQIPRVNGERMEANAIEAAEQCGILTIPSVRAPQGLSAFLGA
RPADDMLVFCDEAAPVADPLVALRPAAGGPITVLIGPEGGFSADERTLLHRQERVIVIALGPRILRADTAGVAALALVQA
VCGDWRPTSG
>Mature_249_residues
PDHDFRAQRLFVREDLAADTTFSLPSEQAHYVANVIRLPVRGGLLVFNGRDGEWRATRIEGGKKEVRLSIGGQTRPQPAA
AGLTYAFAPLKHARLDYMVQKAVEMGAGVLAPVLTQHTQIPRVNGERMEANAIEAAEQCGILTIPSVRAPQGLSAFLGAR
PADDMLVFCDEAAPVADPLVALRPAAGGPITVLIGPEGGFSADERTLLHRQERVIVIALGPRILRADTAGVAALALVQAV
CGDWRPTSG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=247, Percent_Identity=25.9109311740891, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26666; Mature: 26535

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDHDFRAQRLFVREDLAADTTFSLPSEQAHYVANVIRLPVRGGLLVFNGRDGEWRATRI
CCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEE
EGGKKEVRLSIGGQTRPQPAAAGLTYAFAPLKHARLDYMVQKAVEMGAGVLAPVLTQHTQ
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
IPRVNGERMEANAIEAAEQCGILTIPSVRAPQGLSAFLGARPADDMLVFCDEAAPVADPL
CCCCCCCEECHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCC
VALRPAAGGPITVLIGPEGGFSADERTLLHRQERVIVIALGPRILRADTAGVAALALVQA
EEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCEEEECCCCHHHHHHHHHH
VCGDWRPTSG
HHCCCCCCCC
>Mature Secondary Structure 
PDHDFRAQRLFVREDLAADTTFSLPSEQAHYVANVIRLPVRGGLLVFNGRDGEWRATRI
CCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEE
EGGKKEVRLSIGGQTRPQPAAAGLTYAFAPLKHARLDYMVQKAVEMGAGVLAPVLTQHTQ
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
IPRVNGERMEANAIEAAEQCGILTIPSVRAPQGLSAFLGARPADDMLVFCDEAAPVADPL
CCCCCCCEECHHHHHHHHHCCEEEECCCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCC
VALRPAAGGPITVLIGPEGGFSADERTLLHRQERVIVIALGPRILRADTAGVAALALVQA
EEEECCCCCCEEEEECCCCCCCCHHHHHHCCCCCEEEEEECCEEEECCCCHHHHHHHHHH
VCGDWRPTSG
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]