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The map label for this gene is hemF

Identifier: 229587257

GI number: 229587257

Start: 1273804

End: 1274643

Strand: Direct

Name: hemF

Synonym: RAF_ORF1254

Alternate gene names: 229587257

Gene position: 1273804-1274643 (Clockwise)

Preceding gene: 229587256

Following gene: NA

Centisome position: 99.63

GC content: 36.79

Gene sequence:

>840_bases
ATGAACATAGAAAATAAAGAAATAACAAGTAGTTGGTTTACTAATTTACGTGATTTATTGTGCAAAGAATTTGAAAAAAT
CGAAGAAGAATATGCACAAACCAAAGGTTTAAAACCGGCTAAGTTTGTACGCTCAAGTTGGCAACGTAACGGCGGCGGTG
GCGGTGTCATGTCTCTCATGAAAGGAGCAGTATTTGAAAAAGTTGGGGTTAATATATCTACCGTATTTGGTAAAATTTCT
TCCGAATTCCGCAACGAAATTCCAGGAGCAGAGCTAGACGGGAAATTCTTTGCAACAGGCATTTCATTAGTCGCACATCT
TAAATCGCCGCTAATTCCCGCTATGCATTTTAATACTCGTTATATAGAAACTTCTAAAAGTTGGTTTGGCGGCGGTGGCG
ATTTAACACCTTTTTATCCGGAAAAAAACGAAACTGTAAAGTTTCATGCAGCTTTTAAAGAAGCATGCGATAAGTATGAT
TCTAGCTATTATCCTAAATTCAAAAAGCAATGTGATGAATATTTTTATCTAAAGCATAGAAAAGAGCCGAGAGGAGTAGG
CGGAATATTCTATGATTACTTAAATAACGGTAATTTTGAACAGGATTTTGCCTTTACACAGGACGTAGGTAAGGCTTTGT
TGTCGGTATATCCTGAAATCGTTAGAAGTAAGTTATTTTTACCTTGGACAGCTGAGCAGAAAGAATACCAGCTTATAAGG
CGAGGTAGATATGTAGAATTTAATTTGCTATATGACCGCGGTACTAAATTCGGCTTAATGACCGATGGAAATGTCGAAGC
AATATTAATGTCGTTGCCACCTGAAGTAAAGTTTAATTGA

Upstream 100 bases:

>100_bases
TTATTTTATGCTCCGGCTTTTTATATTATGATCATTGCTACCGTATTTTTAGTGATTATGTATATGATGAGGAAAGTAAT
TAAATCGTTGCTTAATAATT

Downstream 100 bases:

>100_bases
TTTTTTTAAAATCATAACCGGAATGGCAAAATGCTATTTCGGTGTGTTGAAATTTCTGTCAAAAGTGATTAGTTAATATA
GCAAATTCATTCTCTTTTGT

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNIENKEITSSWFTNLRDLLCKEFEKIEEEYAQTKGLKPAKFVRSSWQRNGGGGGVMSLMKGAVFEKVGVNISTVFGKIS
SEFRNEIPGAELDGKFFATGISLVAHLKSPLIPAMHFNTRYIETSKSWFGGGGDLTPFYPEKNETVKFHAAFKEACDKYD
SSYYPKFKKQCDEYFYLKHRKEPRGVGGIFYDYLNNGNFEQDFAFTQDVGKALLSVYPEIVRSKLFLPWTAEQKEYQLIR
RGRYVEFNLLYDRGTKFGLMTDGNVEAILMSLPPEVKFN

Sequences:

>Translated_279_residues
MNIENKEITSSWFTNLRDLLCKEFEKIEEEYAQTKGLKPAKFVRSSWQRNGGGGGVMSLMKGAVFEKVGVNISTVFGKIS
SEFRNEIPGAELDGKFFATGISLVAHLKSPLIPAMHFNTRYIETSKSWFGGGGDLTPFYPEKNETVKFHAAFKEACDKYD
SSYYPKFKKQCDEYFYLKHRKEPRGVGGIFYDYLNNGNFEQDFAFTQDVGKALLSVYPEIVRSKLFLPWTAEQKEYQLIR
RGRYVEFNLLYDRGTKFGLMTDGNVEAILMSLPPEVKFN
>Mature_279_residues
MNIENKEITSSWFTNLRDLLCKEFEKIEEEYAQTKGLKPAKFVRSSWQRNGGGGGVMSLMKGAVFEKVGVNISTVFGKIS
SEFRNEIPGAELDGKFFATGISLVAHLKSPLIPAMHFNTRYIETSKSWFGGGGDLTPFYPEKNETVKFHAAFKEACDKYD
SSYYPKFKKQCDEYFYLKHRKEPRGVGGIFYDYLNNGNFEQDFAFTQDVGKALLSVYPEIVRSKLFLPWTAEQKEYQLIR
RGRYVEFNLLYDRGTKFGLMTDGNVEAILMSLPPEVKFN

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=275, Percent_Identity=45.0909090909091, Blast_Score=224, Evalue=6e-59,
Organism=Escherichia coli, GI1788777, Length=275, Percent_Identity=42.5454545454545, Blast_Score=225, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6320249, Length=269, Percent_Identity=45.724907063197, Blast_Score=218, Evalue=8e-58,
Organism=Drosophila melanogaster, GI17864388, Length=254, Percent_Identity=46.0629921259843, Blast_Score=225, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_RICAE (C3PM25)

Other databases:

- EMBL:   CP001612
- RefSeq:   YP_002845758.1
- GeneID:   7815844
- GenomeReviews:   CP001612_GR
- KEGG:   raf:RAF_ORF1254
- OMA:   HMNTRFV
- ProtClustDB:   PRK05330
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 31973; Mature: 31973

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 116-116 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIENKEITSSWFTNLRDLLCKEFEKIEEEYAQTKGLKPAKFVRSSWQRNGGGGGVMSLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
KGAVFEKVGVNISTVFGKISSEFRNEIPGAELDGKFFATGISLVAHLKSPLIPAMHFNTR
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCE
YIETSKSWFGGGGDLTPFYPEKNETVKFHAAFKEACDKYDSSYYPKFKKQCDEYFYLKHR
EEECCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
KEPRGVGGIFYDYLNNGNFEQDFAFTQDVGKALLSVYPEIVRSKLFLPWTAEQKEYQLIR
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RGRYVEFNLLYDRGTKFGLMTDGNVEAILMSLPPEVKFN
CCCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MNIENKEITSSWFTNLRDLLCKEFEKIEEEYAQTKGLKPAKFVRSSWQRNGGGGGVMSLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHH
KGAVFEKVGVNISTVFGKISSEFRNEIPGAELDGKFFATGISLVAHLKSPLIPAMHFNTR
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCE
YIETSKSWFGGGGDLTPFYPEKNETVKFHAAFKEACDKYDSSYYPKFKKQCDEYFYLKHR
EEECCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
KEPRGVGGIFYDYLNNGNFEQDFAFTQDVGKALLSVYPEIVRSKLFLPWTAEQKEYQLIR
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
RGRYVEFNLLYDRGTKFGLMTDGNVEAILMSLPPEVKFN
CCCEEEEEEEEECCCEEEEEECCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA