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The map label for this gene is proP7 [H]

Identifier: 229587256

GI number: 229587256

Start: 1272546

End: 1273820

Strand: Direct

Name: proP7 [H]

Synonym: RAF_ORF1253

Alternate gene names: 229587256

Gene position: 1272546-1273820 (Clockwise)

Preceding gene: 229587248

Following gene: 229587257

Centisome position: 99.53

GC content: 35.92

Gene sequence:

>1275_bases
ATGAATAATAGGTCTAAGTTCATTTTTTTATCTGCTATTTCAGGTAATGTACTCGAATATTATGACTTTACGGTATATTC
AGTTTTTTCGCTGATTATTGGACAAGTTTTTTTTCCAAGCGAATCAGAATTCATTAGAATTCTTCTAAGCCTTGGAGTAT
TTGCGGTTGGCTTTCTGACAAGACCGATAGGAGGCATATTATTCGGTTATATCGGTGATAGATACGGTAGACGTATCGCT
TTAATAATCTCGATGCTAGGTATGACTATTCCGACCTTTATTATGGGTCTCATACCCTCATATGCAAGTATTGGGATTTA
TGCCCCTATAACTCTAGTTATAATGCGGCTTATTCAAGGTTTATGTATTAGCGGTGAAGGAACGGGAGCGGCTATTTTTA
TCCTTGAACATCGTCAAAATTTAAGACCGGGTTTTACGGCAGGTTTAGTACATGGTTCAAATATCGCCGGTACCTTAATT
GCAACATTTATCGGTATTATAATAGAACGTTATTTCTCTCATATAGATTTTGCTTGGCGTTTTGCTTTTCTGCTTGGCGG
GTTTATGGGTCTTGCAGGATTTTACTTGCGATTGCGCGTATCGGAAACACCGATTTTTAAAATGCTTGAGAAAAAGAAAC
AAGTTCTTAAAGCACCTTTTTCCAATGTAATTAGAACTGCTTGGAGATCGATATTTTTAACTATGTGTATAGGTGCTATC
GCTAGCAGCGTTATGTATTTAGTAAAAACATATATAAATGTTTTCTATTATAATGTAATGCATCTTAGTAATACTATTGC
TTTATCATATTTAGCGTATAGTTCGTTTATTGCGATGATAGCGATGCCGCTTGCCGGCGGTACTGCCGATATTATCGGAA
AATTTAAAATGGCAATACTTGTTGGTGTTGCTATCTTGATATTGATTTTACCGACCATGTTACTTATGTCGACAGAAGAG
ATGTGGCAACAAATTATAGCTCTTACAATGCTTGGTATGCTCGCAGGAAGCATAGCAGGTACAGCTTATATACTCGTTAT
ATCCTTGTTTACGGCAGAACAAAGATTTACCGGTGTTGCTTTCAGCTACAATTTTGCAATAGCGATATTCGGCGGAACTT
CACCTATTATTTCTCGTTGGCTTGTAGAGCACACAGGCTTATTTTATGCTCCGGCTTTTTATATTATGATCATTGCTACC
GTATTTTTAGTGATTATGTATATGATGAGGAAAGTAATTAAATCGTTGCTTAATAATTATGAACATAGAAAATAA

Upstream 100 bases:

>100_bases
AAGAATTAAAATGCCGACAATTAAATATTATAAATTACACTTTATAAATTATGTAACTTGTCTATAATAACTAATTAGTA
ATTAAGTTATTGAAAGCTCG

Downstream 100 bases:

>100_bases
AGAAATAACAAGTAGTTGGTTTACTAATTTACGTGATTTATTGTGCAAAGAATTTGAAAAAATCGAAGAAGAATATGCAC
AAACCAAAGGTTTAAAACCG

Product: Proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MNNRSKFIFLSAISGNVLEYYDFTVYSVFSLIIGQVFFPSESEFIRILLSLGVFAVGFLTRPIGGILFGYIGDRYGRRIA
LIISMLGMTIPTFIMGLIPSYASIGIYAPITLVIMRLIQGLCISGEGTGAAIFILEHRQNLRPGFTAGLVHGSNIAGTLI
ATFIGIIIERYFSHIDFAWRFAFLLGGFMGLAGFYLRLRVSETPIFKMLEKKKQVLKAPFSNVIRTAWRSIFLTMCIGAI
ASSVMYLVKTYINVFYYNVMHLSNTIALSYLAYSSFIAMIAMPLAGGTADIIGKFKMAILVGVAILILILPTMLLMSTEE
MWQQIIALTMLGMLAGSIAGTAYILVISLFTAEQRFTGVAFSYNFAIAIFGGTSPIISRWLVEHTGLFYAPAFYIMIIAT
VFLVIMYMMRKVIKSLLNNYEHRK

Sequences:

>Translated_424_residues
MNNRSKFIFLSAISGNVLEYYDFTVYSVFSLIIGQVFFPSESEFIRILLSLGVFAVGFLTRPIGGILFGYIGDRYGRRIA
LIISMLGMTIPTFIMGLIPSYASIGIYAPITLVIMRLIQGLCISGEGTGAAIFILEHRQNLRPGFTAGLVHGSNIAGTLI
ATFIGIIIERYFSHIDFAWRFAFLLGGFMGLAGFYLRLRVSETPIFKMLEKKKQVLKAPFSNVIRTAWRSIFLTMCIGAI
ASSVMYLVKTYINVFYYNVMHLSNTIALSYLAYSSFIAMIAMPLAGGTADIIGKFKMAILVGVAILILILPTMLLMSTEE
MWQQIIALTMLGMLAGSIAGTAYILVISLFTAEQRFTGVAFSYNFAIAIFGGTSPIISRWLVEHTGLFYAPAFYIMIIAT
VFLVIMYMMRKVIKSLLNNYEHRK
>Mature_424_residues
MNNRSKFIFLSAISGNVLEYYDFTVYSVFSLIIGQVFFPSESEFIRILLSLGVFAVGFLTRPIGGILFGYIGDRYGRRIA
LIISMLGMTIPTFIMGLIPSYASIGIYAPITLVIMRLIQGLCISGEGTGAAIFILEHRQNLRPGFTAGLVHGSNIAGTLI
ATFIGIIIERYFSHIDFAWRFAFLLGGFMGLAGFYLRLRVSETPIFKMLEKKKQVLKAPFSNVIRTAWRSIFLTMCIGAI
ASSVMYLVKTYINVFYYNVMHLSNTIALSYLAYSSFIAMIAMPLAGGTADIIGKFKMAILVGVAILILILPTMLLMSTEE
MWQQIIALTMLGMLAGSIAGTAYILVISLFTAEQRFTGVAFSYNFAIAIFGGTSPIISRWLVEHTGLFYAPAFYIMIIAT
VFLVIMYMMRKVIKSLLNNYEHRK

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=31.6784869976359, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1788942, Length=421, Percent_Identity=30.166270783848, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=29.3233082706767, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788292, Length=408, Percent_Identity=25.9803921568627, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI87082231, Length=437, Percent_Identity=23.1121281464531, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI87082404, Length=340, Percent_Identity=24.4117647058824, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47169; Mature: 47169

Theoretical pI: Translated: 10.01; Mature: 10.01

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNRSKFIFLSAISGNVLEYYDFTVYSVFSLIIGQVFFPSESEFIRILLSLGVFAVGFLT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RPIGGILFGYIGDRYGRRIALIISMLGMTIPTFIMGLIPSYASIGIYAPITLVIMRLIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LCISGEGTGAAIFILEHRQNLRPGFTAGLVHGSNIAGTLIATFIGIIIERYFSHIDFAWR
CEECCCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFLLGGFMGLAGFYLRLRVSETPIFKMLEKKKQVLKAPFSNVIRTAWRSIFLTMCIGAI
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
ASSVMYLVKTYINVFYYNVMHLSNTIALSYLAYSSFIAMIAMPLAGGTADIIGKFKMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGVAILILILPTMLLMSTEEMWQQIIALTMLGMLAGSIAGTAYILVISLFTAEQRFTGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSYNFAIAIFGGTSPIISRWLVEHTGLFYAPAFYIMIIATVFLVIMYMMRKVIKSLLNNY
EEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EHRK
CCCC
>Mature Secondary Structure
MNNRSKFIFLSAISGNVLEYYDFTVYSVFSLIIGQVFFPSESEFIRILLSLGVFAVGFLT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
RPIGGILFGYIGDRYGRRIALIISMLGMTIPTFIMGLIPSYASIGIYAPITLVIMRLIQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LCISGEGTGAAIFILEHRQNLRPGFTAGLVHGSNIAGTLIATFIGIIIERYFSHIDFAWR
CEECCCCCCEEEEEEECCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FAFLLGGFMGLAGFYLRLRVSETPIFKMLEKKKQVLKAPFSNVIRTAWRSIFLTMCIGAI
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
ASSVMYLVKTYINVFYYNVMHLSNTIALSYLAYSSFIAMIAMPLAGGTADIIGKFKMAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VGVAILILILPTMLLMSTEEMWQQIIALTMLGMLAGSIAGTAYILVISLFTAEQRFTGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
FSYNFAIAIFGGTSPIISRWLVEHTGLFYAPAFYIMIIATVFLVIMYMMRKVIKSLLNNY
EEEEEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EHRK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]