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The map label for this gene is map [H]

Identifier: 229587188

GI number: 229587188

Start: 1195502

End: 1196281

Strand: Direct

Name: map [H]

Synonym: RAF_ORF1169

Alternate gene names: 229587188

Gene position: 1195502-1196281 (Clockwise)

Preceding gene: 229587181

Following gene: 229587189

Centisome position: 93.51

GC content: 37.44

Gene sequence:

>780_bases
ATGACCATAAAAATCCACACGGGAAAAGATTTCATAAAGATGCGGGCAGCAGGAAAATTAGCTGCAGAGACGCTCGATTT
TATTACTGATCACGTAAAACCGAATGTCACTACTAATAGTTTAAATGGCCTTTGTCATAATTTTATTACTTCTCATAATG
CTGTTCCTGCACCGTTAAATTACAAAGGCTTTCCGAAATCGATTTGTACTTCTATAAATCATGTGGTTTGTCACGGCATT
CCAAACGATAAACCGTTAAAAAACGGTGATATAGTAAATATTGATGTTACGGTAATTTTAGACGGTTGGTACGGTGATAC
TAGCCGTATGTATTATGTCGGTGACGTAGCAATTAAGCCAAAGCGTCTTATTCAAGTAACTTATGATGCAATGATGAAAG
GGATTGAAGTAGTTAGACCCGGTGCTAAACTCGGTGATATCGGACATGCTATTCAAAGCTATGCCGAGAAACATAATTAT
TCTGTGGTTAGAGATTATACGGGTCACGGTATCGGAAGAGTTTTTCATGACGAACCATCAATATTAAATTACGGTCGCAG
CGGCACGGGTCTAACGTTAGAAGAAGGGATGTTTTTCACCGTTGAACCTATGATAAATGCCGGTAATTATGACACTATAT
TAAGTAAACTAGATGGATGGACAGTTACTACACGTGATAAATCATTATCGGCTCAATTTGAGCATACTATAGGCGTGACT
AAAGACGGTTTTGAAATATTTACGTTATCACCTAAAAAACTTGATTATCCTCCATACTAA

Upstream 100 bases:

>100_bases
TAGCAGCACTTCTCACAATAATAGGATAAGTTATTTACATTAAAAAAGCCATTTTGTATTATGAAAATATATTTTTTTGA
GGGAAAGGTAACCAAAATTC

Downstream 100 bases:

>100_bases
ACTGTAGGGTAAAACTTGATTTTGTTGTATGGCTATCTATGTCATTCCCGCGGGACATTTTTGCGTGGATCAAAAAACGC
ACTCAGTGTCATCCCGTGGC

Product: methionine aminopeptidase

Products: NA

Alternate protein names: MAP; Peptidase M [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTIKIHTGKDFIKMRAAGKLAAETLDFITDHVKPNVTTNSLNGLCHNFITSHNAVPAPLNYKGFPKSICTSINHVVCHGI
PNDKPLKNGDIVNIDVTVILDGWYGDTSRMYYVGDVAIKPKRLIQVTYDAMMKGIEVVRPGAKLGDIGHAIQSYAEKHNY
SVVRDYTGHGIGRVFHDEPSILNYGRSGTGLTLEEGMFFTVEPMINAGNYDTILSKLDGWTVTTRDKSLSAQFEHTIGVT
KDGFEIFTLSPKKLDYPPY

Sequences:

>Translated_259_residues
MTIKIHTGKDFIKMRAAGKLAAETLDFITDHVKPNVTTNSLNGLCHNFITSHNAVPAPLNYKGFPKSICTSINHVVCHGI
PNDKPLKNGDIVNIDVTVILDGWYGDTSRMYYVGDVAIKPKRLIQVTYDAMMKGIEVVRPGAKLGDIGHAIQSYAEKHNY
SVVRDYTGHGIGRVFHDEPSILNYGRSGTGLTLEEGMFFTVEPMINAGNYDTILSKLDGWTVTTRDKSLSAQFEHTIGVT
KDGFEIFTLSPKKLDYPPY
>Mature_258_residues
TIKIHTGKDFIKMRAAGKLAAETLDFITDHVKPNVTTNSLNGLCHNFITSHNAVPAPLNYKGFPKSICTSINHVVCHGIP
NDKPLKNGDIVNIDVTVILDGWYGDTSRMYYVGDVAIKPKRLIQVTYDAMMKGIEVVRPGAKLGDIGHAIQSYAEKHNYS
VVRDYTGHGIGRVFHDEPSILNYGRSGTGLTLEEGMFFTVEPMINAGNYDTILSKLDGWTVTTRDKSLSAQFEHTIGVTK
DGFEIFTLSPKKLDYPPY

Specific function: Removes the amino-terminal methionine from nascent proteins [H]

COG id: COG0024

COG function: function code J; Methionine aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24A family [H]

Homologues:

Organism=Homo sapiens, GI164420681, Length=253, Percent_Identity=42.2924901185771, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI40385867, Length=252, Percent_Identity=40.0793650793651, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1786364, Length=251, Percent_Identity=53.7848605577689, Blast_Score=270, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI71996291, Length=247, Percent_Identity=41.2955465587045, Blast_Score=204, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6323273, Length=252, Percent_Identity=41.2698412698413, Blast_Score=211, Evalue=8e-56,
Organism=Drosophila melanogaster, GI21355531, Length=246, Percent_Identity=45.5284552845528, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI24583427, Length=235, Percent_Identity=41.7021276595745, Blast_Score=197, Evalue=7e-51,

Paralogues:

None

Copy number: 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR002467 [H]

Pfam domain/function: PF00557 Peptidase_M24 [H]

EC number: =3.4.11.18 [H]

Molecular weight: Translated: 28666; Mature: 28535

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS00680 MAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKIHTGKDFIKMRAAGKLAAETLDFITDHVKPNVTTNSLNGLCHNFITSHNAVPAPLN
CEEEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKGFPKSICTSINHVVCHGIPNDKPLKNGDIVNIDVTVILDGWYGDTSRMYYVGDVAIKP
CCCCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECC
KRLIQVTYDAMMKGIEVVRPGAKLGDIGHAIQSYAEKHNYSVVRDYTGHGIGRVFHDEPS
HHHEEEEHHHHHCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCC
ILNYGRSGTGLTLEEGMFFTVEPMINAGNYDTILSKLDGWTVTTRDKSLSAQFEHTIGVT
HHHCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHCCCCC
KDGFEIFTLSPKKLDYPPY
CCCEEEEEECCCCCCCCCC
>Mature Secondary Structure 
TIKIHTGKDFIKMRAAGKLAAETLDFITDHVKPNVTTNSLNGLCHNFITSHNAVPAPLN
EEEEECCCHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
YKGFPKSICTSINHVVCHGIPNDKPLKNGDIVNIDVTVILDGWYGDTSRMYYVGDVAIKP
CCCCCHHHHHCCCEEEEECCCCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECC
KRLIQVTYDAMMKGIEVVRPGAKLGDIGHAIQSYAEKHNYSVVRDYTGHGIGRVFHDEPS
HHHEEEEHHHHHCCHHEECCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEECCCCC
ILNYGRSGTGLTLEEGMFFTVEPMINAGNYDTILSKLDGWTVTTRDKSLSAQFEHTIGVT
HHHCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCEEEECCCCCCCCHHHCCCCC
KDGFEIFTLSPKKLDYPPY
CCCEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]