| Definition | Rickettsia africae ESF-5 chromosome, complete genome. |
|---|---|
| Accession | NC_012633 |
| Length | 1,278,540 |
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The map label for this gene is virB4-2 [H]
Identifier: 229587146
GI number: 229587146
Start: 1127518
End: 1129950
Strand: Direct
Name: virB4-2 [H]
Synonym: RAF_ORF1109
Alternate gene names: 229587146
Gene position: 1127518-1129950 (Clockwise)
Preceding gene: 229587145
Following gene: 229587147
Centisome position: 88.19
GC content: 27.58
Gene sequence:
>2433_bases GTGTTTAGTGGTTTACGAAAATTTGATAAAGATCTATATAATTATAAGGCTCCTAATTTTATACCTATAGCATGTCATTA TAATGAAAATACTTTACTTACCAAAGACGGTAAATTACTACAAATTATTAAAATACACGGCATTACCTCAGAGAAAATAA GCGATAACATACAAAATTTACGTGAAATGGTTAGAGTTTCTATAACAAAGAATATAACCGATTATGATTTTGCTTTTTGG CTACATACAATACGAGAAAAACAAAACTTAGACGATTCAACTCCTTACAAAAAATTATTACCGGCAAATATTCATACCTT ATGGCAAAGAAAAAACCATTGGAATAATAAATTCGTTAATACTTTATATATATCTATAGTACATGATTCTACTAAAGTTA ACATCACAAATTTTAACTCTTTAATAAATTCTTTATCTCATAAATTAATTACTGATTTTGAAAATAACTACTTAGATTCA GCTTTTCAAAAATTAGACAATATCACTAATAATATTTTAAATGATTTAAACGAATTCAGTGCTGAAAAACTCGGTATAAT ATTTGAAAATGATAATGTTTTTTCTAATCCTTTATTTTTATATAATCGCATAGCTAACCTTAATAATAATGATTGTTTGA TACCTATAATAGATTTATCCGATGCAATAGGTAGAAGTATTTATACAATTAGCAGCGATAAAATGGTAGTTACGGATCAT GAGAAAAATAATAAATTCGTTTCTTTACTATCTATAAAAGAATATCATGAAACTCCTTCTAATACACTGGATAAATTTTT ACAGTTACCGATTGAATTAATAATAACTGAAATATTTTACTTTGTTAGCAAAAAACAAGTAATATCCAAATTGCATGGTC AAGATTATATTCTAAAAATTACTAACGACTCAACTTTACTTAATCACAAAGGGATTAATAAGCTTGATGCAGCTAATTTA GATTTTCAATTTTGTAATCAGCAAATTTCAATTGCCGTTATAGAAGAAGATGAAAATAAACTAGATGCAGCCGTAGCTAA AGCATCAACCGAACTTTTTAAACTTGGTATTATTCATGTAAAAGAAGATATTAATATTGAGCAAATATTTTGGTCGCAAT TACCTGCTAACTTTGCCTTTATACGTAGAATGTCGCCATTATCCGTAGAGCATATTGCTTCTCTTACCGCTCTTCATAAT ACTACACTCGGTAATCAATATAATCCTTGGGGTAAGGCTATAACTTTACTGCGAACCGAGAAAGGTACTCCATATTTCAT GAATTTTCATGATAAAACGAATAAAGGCAATACTTGCATCTTTGGTACGGGGAAAACCGGCAAAACCGTATTATTGAACT TTTTAATATCAGAAGCGACTAAATACGATCCAACAATAATTTATATCTCTAATAATAATGATTCTAAAATTTTTATCGAG GCAATAGAAGGCAAATGGTTAGAACCGGACAAACAAATTATCAATCCATTTTTAGTAGATGATACAGAAAAATCACAAGC ATTCATTTTAGAATTTTTAAAATTAATCAGTGGTCATTATATTTCACCTCTTAGCGAAATAGAGATATCCTTTCTAGAGA AATTAAAAAATAAAATTTTATCAATTGAAAAAGAAAAACGTATTTTTTCCGATATTTTAAAATTAGAAGATTTTAAGGAG GAAGGAGGAATACAAATACTTGATAAACTTAAAGTTTTTACCGAAGAACAATTATATTCCGGATTATTTGACGGATCGTC TCTTAACATTGAAGAAGGGAAAGTTATAGGGTTTAATTTATATAAACTTTCTGATGAGCCGTTTTCTAAACAATTTTACC CGACGGAGAGAAAATTTTTAGAACAATTTAATAATAATTTAAAGAAACACCAAAGTATTAGTGCAGCAGTAATTTATGCT TTTACTTATCATTTAAGTCTAGTTGGCACAAAACCTAAAATATTTGCTGCCGATAATTTTGATAAACTTTACAGACCTGA GATTTATTATGATAATATAAATTTAATCTATAATAATTTGTCTCAAAATAACGGTATTTTCGTCAGTAATTTCAATTTTA TTTATCTTAAGTCATATCCAAAATATACTATAAAACCTTGGCTTGATTTAATTAATACTAAAATTATTCTACCATCTGAC GTTAAAATAGAAGATTTAGATAAAATTTTAGGTTTAAGCAAACCGGAAATACGAAAATTATCACAGCTAATACTTTCTGC AAGAATGTTCTTAATTAGTAAGGATAATGAATCGATAGCTTCTGAATTAAGTATTGCCGCTTTAATAGGAGTTGTCCGTA TTTTATCAAGCAGACAGGAGGAAATGGATATTTATAAAAAAATACTTGAACAACACCAAGGTCCTCCAGATAATTGGGTT AATTACCTATATAATGAGTTAAATACGGACTAA
Upstream 100 bases:
>100_bases TATACTTAATACGTGAGCATCCAAGGACGTACAAAGACGACGTAGCCAATTTTTCAAGTTGAACGAGTATACAAAACGAT AATTTAAATTCAGGTTATTT
Downstream 100 bases:
>100_bases TATTTCACTCTGTCATCCCGTGATTTATTATGGGATCCAAAAAAAATTAAAAAGGATTGGATTCCGCTACAAGCTCGTGG GATGACAATAATTTATTATG
Product: VirB4
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 810; Mature: 810
Protein sequence:
>810_residues MFSGLRKFDKDLYNYKAPNFIPIACHYNENTLLTKDGKLLQIIKIHGITSEKISDNIQNLREMVRVSITKNITDYDFAFW LHTIREKQNLDDSTPYKKLLPANIHTLWQRKNHWNNKFVNTLYISIVHDSTKVNITNFNSLINSLSHKLITDFENNYLDS AFQKLDNITNNILNDLNEFSAEKLGIIFENDNVFSNPLFLYNRIANLNNNDCLIPIIDLSDAIGRSIYTISSDKMVVTDH EKNNKFVSLLSIKEYHETPSNTLDKFLQLPIELIITEIFYFVSKKQVISKLHGQDYILKITNDSTLLNHKGINKLDAANL DFQFCNQQISIAVIEEDENKLDAAVAKASTELFKLGIIHVKEDINIEQIFWSQLPANFAFIRRMSPLSVEHIASLTALHN TTLGNQYNPWGKAITLLRTEKGTPYFMNFHDKTNKGNTCIFGTGKTGKTVLLNFLISEATKYDPTIIYISNNNDSKIFIE AIEGKWLEPDKQIINPFLVDDTEKSQAFILEFLKLISGHYISPLSEIEISFLEKLKNKILSIEKEKRIFSDILKLEDFKE EGGIQILDKLKVFTEEQLYSGLFDGSSLNIEEGKVIGFNLYKLSDEPFSKQFYPTERKFLEQFNNNLKKHQSISAAVIYA FTYHLSLVGTKPKIFAADNFDKLYRPEIYYDNINLIYNNLSQNNGIFVSNFNFIYLKSYPKYTIKPWLDLINTKIILPSD VKIEDLDKILGLSKPEIRKLSQLILSARMFLISKDNESIASELSIAALIGVVRILSSRQEEMDIYKKILEQHQGPPDNWV NYLYNELNTD
Sequences:
>Translated_810_residues MFSGLRKFDKDLYNYKAPNFIPIACHYNENTLLTKDGKLLQIIKIHGITSEKISDNIQNLREMVRVSITKNITDYDFAFW LHTIREKQNLDDSTPYKKLLPANIHTLWQRKNHWNNKFVNTLYISIVHDSTKVNITNFNSLINSLSHKLITDFENNYLDS AFQKLDNITNNILNDLNEFSAEKLGIIFENDNVFSNPLFLYNRIANLNNNDCLIPIIDLSDAIGRSIYTISSDKMVVTDH EKNNKFVSLLSIKEYHETPSNTLDKFLQLPIELIITEIFYFVSKKQVISKLHGQDYILKITNDSTLLNHKGINKLDAANL DFQFCNQQISIAVIEEDENKLDAAVAKASTELFKLGIIHVKEDINIEQIFWSQLPANFAFIRRMSPLSVEHIASLTALHN TTLGNQYNPWGKAITLLRTEKGTPYFMNFHDKTNKGNTCIFGTGKTGKTVLLNFLISEATKYDPTIIYISNNNDSKIFIE AIEGKWLEPDKQIINPFLVDDTEKSQAFILEFLKLISGHYISPLSEIEISFLEKLKNKILSIEKEKRIFSDILKLEDFKE EGGIQILDKLKVFTEEQLYSGLFDGSSLNIEEGKVIGFNLYKLSDEPFSKQFYPTERKFLEQFNNNLKKHQSISAAVIYA FTYHLSLVGTKPKIFAADNFDKLYRPEIYYDNINLIYNNLSQNNGIFVSNFNFIYLKSYPKYTIKPWLDLINTKIILPSD VKIEDLDKILGLSKPEIRKLSQLILSARMFLISKDNESIASELSIAALIGVVRILSSRQEEMDIYKKILEQHQGPPDNWV NYLYNELNTD >Mature_810_residues MFSGLRKFDKDLYNYKAPNFIPIACHYNENTLLTKDGKLLQIIKIHGITSEKISDNIQNLREMVRVSITKNITDYDFAFW LHTIREKQNLDDSTPYKKLLPANIHTLWQRKNHWNNKFVNTLYISIVHDSTKVNITNFNSLINSLSHKLITDFENNYLDS AFQKLDNITNNILNDLNEFSAEKLGIIFENDNVFSNPLFLYNRIANLNNNDCLIPIIDLSDAIGRSIYTISSDKMVVTDH EKNNKFVSLLSIKEYHETPSNTLDKFLQLPIELIITEIFYFVSKKQVISKLHGQDYILKITNDSTLLNHKGINKLDAANL DFQFCNQQISIAVIEEDENKLDAAVAKASTELFKLGIIHVKEDINIEQIFWSQLPANFAFIRRMSPLSVEHIASLTALHN TTLGNQYNPWGKAITLLRTEKGTPYFMNFHDKTNKGNTCIFGTGKTGKTVLLNFLISEATKYDPTIIYISNNNDSKIFIE AIEGKWLEPDKQIINPFLVDDTEKSQAFILEFLKLISGHYISPLSEIEISFLEKLKNKILSIEKEKRIFSDILKLEDFKE EGGIQILDKLKVFTEEQLYSGLFDGSSLNIEEGKVIGFNLYKLSDEPFSKQFYPTERKFLEQFNNNLKKHQSISAAVIYA FTYHLSLVGTKPKIFAADNFDKLYRPEIYYDNINLIYNNLSQNNGIFVSNFNFIYLKSYPKYTIKPWLDLINTKIILPSD VKIEDLDKILGLSKPEIRKLSQLILSARMFLISKDNESIASELSIAALIGVVRILSSRQEEMDIYKKILEQHQGPPDNWV NYLYNELNTD
Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-
COG id: COG3451
COG function: function code U; Type IV secretory pathway, VirB4 components
Gene ontology:
Cell location: Cell inner membrane (Potential) [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: Belongs to the trbE/virB4 family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003593 - InterPro: IPR004346 - InterPro: IPR018145 [H]
Pfam domain/function: PF03135 CagE_TrbE_VirB [H]
EC number: NA
Molecular weight: Translated: 93463; Mature: 93463
Theoretical pI: Translated: 6.48; Mature: 6.48
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 0.9 %Met (Translated Protein) 1.4 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 0.9 %Met (Mature Protein) 1.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MFSGLRKFDKDLYNYKAPNFIPIACHYNENTLLTKDGKLLQIIKIHGITSEKISDNIQNL CCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCHHHHHHHHHHH REMVRVSITKNITDYDFAFWLHTIREKQNLDDSTPYKKLLPANIHTLWQRKNHWNNKFVN HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEE TLYISIVHDSTKVNITNFNSLINSLSHKLITDFENNYLDSAFQKLDNITNNILNDLNEFS EEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC AEKLGIIFENDNVFSNPLFLYNRIANLNNNDCLIPIIDLSDAIGRSIYTISSDKMVVTDH HHHEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHCCEEEEECCCEEEEEEC EKNNKFVSLLSIKEYHETPSNTLDKFLQLPIELIITEIFYFVSKKQVISKLHGQDYILKI CCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE TNDSTLLNHKGINKLDAANLDFQFCNQQISIAVIEEDENKLDAAVAKASTELFKLGIIHV CCCCEEECCCCCCCCCCCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEE KEDINIEQIFWSQLPANFAFIRRMSPLSVEHIASLTALHNTTLGNQYNPWGKAITLLRTE ECCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEC KGTPYFMNFHDKTNKGNTCIFGTGKTGKTVLLNFLISEATKYDPTIIYISNNNDSKIFIE CCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE AIEGKWLEPDKQIINPFLVDDTEKSQAFILEFLKLISGHYISPLSEIEISFLEKLKNKIL EECCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH SIEKEKRIFSDILKLEDFKEEGGIQILDKLKVFTEEQLYSGLFDGSSLNIEEGKVIGFNL CHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE YKLSDEPFSKQFYPTERKFLEQFNNNLKKHQSISAAVIYAFTYHLSLVGTKPKIFAADNF EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHEEEEEHEEEEECCCCEEEECCCC DKLYRPEIYYDNINLIYNNLSQNNGIFVSNFNFIYLKSYPKYTIKPWLDLINTKIILPSD HHHCCCEEEECCEEEEEECCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCEEECCCC VKIEDLDKILGLSKPEIRKLSQLILSARMFLISKDNESIASELSIAALIGVVRILSSRQE CCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCHH EMDIYKKILEQHQGPPDNWVNYLYNELNTD HHHHHHHHHHHCCCCCHHHHHHHHHHHCCC >Mature Secondary Structure MFSGLRKFDKDLYNYKAPNFIPIACHYNENTLLTKDGKLLQIIKIHGITSEKISDNIQNL CCCHHHHHHHHHHHCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCHHHHHHHHHHH REMVRVSITKNITDYDFAFWLHTIREKQNLDDSTPYKKLLPANIHTLWQRKNHWNNKFVN HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHCCCCCCEEE TLYISIVHDSTKVNITNFNSLINSLSHKLITDFENNYLDSAFQKLDNITNNILNDLNEFS EEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC AEKLGIIFENDNVFSNPLFLYNRIANLNNNDCLIPIIDLSDAIGRSIYTISSDKMVVTDH HHHEEEEEECCCCCCCCHHHHHHHCCCCCCCCEEEEECCCHHHCCEEEEECCCEEEEEEC EKNNKFVSLLSIKEYHETPSNTLDKFLQLPIELIITEIFYFVSKKQVISKLHGQDYILKI CCCCCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE TNDSTLLNHKGINKLDAANLDFQFCNQQISIAVIEEDENKLDAAVAKASTELFKLGIIHV CCCCEEECCCCCCCCCCCCCCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHEEEEEE KEDINIEQIFWSQLPANFAFIRRMSPLSVEHIASLTALHNTTLGNQYNPWGKAITLLRTE ECCCCHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHEEEEEEEC KGTPYFMNFHDKTNKGNTCIFGTGKTGKTVLLNFLISEATKYDPTIIYISNNNDSKIFIE CCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEE AIEGKWLEPDKQIINPFLVDDTEKSQAFILEFLKLISGHYISPLSEIEISFLEKLKNKIL EECCCCCCCCHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH SIEKEKRIFSDILKLEDFKEEGGIQILDKLKVFTEEQLYSGLFDGSSLNIEEGKVIGFNL CHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEE YKLSDEPFSKQFYPTERKFLEQFNNNLKKHQSISAAVIYAFTYHLSLVGTKPKIFAADNF EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHEEEEEHEEEEECCCCEEEECCCC DKLYRPEIYYDNINLIYNNLSQNNGIFVSNFNFIYLKSYPKYTIKPWLDLINTKIILPSD HHHCCCEEEECCEEEEEECCCCCCCEEEECCCEEEEECCCCCCHHHHHHHHCCEEECCCC VKIEDLDKILGLSKPEIRKLSQLILSARMFLISKDNESIASELSIAALIGVVRILSSRQE CCHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHCCHH EMDIYKKILEQHQGPPDNWVNYLYNELNTD HHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: 10749166; 10882236; 7494028 [H]