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The map label for this gene is grpE [H]

Identifier: 229586978

GI number: 229586978

Start: 924941

End: 925477

Strand: Direct

Name: grpE [H]

Synonym: RAF_ORF0878

Alternate gene names: 229586978

Gene position: 924941-925477 (Clockwise)

Preceding gene: 229586973

Following gene: 229586979

Centisome position: 72.34

GC content: 33.15

Gene sequence:

>537_bases
ATGATAGACGATAATATAGAAAATAATGAACAAACAATAAATGATATTGCTGAAGAGATAGTTGAAACAGCAAATCCGGA
GGTAACAGCGTTAAAAGCCGAGATTGAAGAGCTAAAAGATAAGCTAATAAGAACTACTGCTGAAATAGACAATACAAGAA
AGCGTTTAGAAAAAGCACGTGACGAAGCAAAAGATTATGCAATTGCTACATTTGCTAAAGAACTACTAAATGTTAGTGAT
AATCTTTCAAGAGCATTAGCACATAAACCGGCAAATTCTGATGTAGAAGTCACAAATATTATTGCAGGCGTTCAGATGAC
TAAAGACGAGCTAGACAAAGTCTTTCATAAACATCATATTGAAGAAATAAAGCCGGAAATAGGATCAATGTTCGACTATA
ATTTACATAATGCAATTGCGCAGATAGAACATCCTGATCATGCACCGAATAGTATTATTACTTTAATGCAATCAGGATAT
AAAATTAGAGATAGACTACTTCGTCCTGCTACTGTACAAGTAGTTAAAAAACCTTAA

Upstream 100 bases:

>100_bases
AACAACGCCAATTTTTCAAATTAAACGAGTATACCATTGAAAAAATTTTTATTATATCTATATATATTTCAAAAAAGAAC
ATAAATATAAAGTAAATAAT

Downstream 100 bases:

>100_bases
TACTCGATCAACTTCAAAAATTGGCGTCGTCGTTTTATAAGAACTTCAATTCTCACGTATTAAATATATGCTGTGATCTT
CGCCTTGTGAACTCCTAGCT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MIDDNIENNEQTINDIAEEIVETANPEVTALKAEIEELKDKLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDVEVTNIIAGVQMTKDELDKVFHKHHIEEIKPEIGSMFDYNLHNAIAQIEHPDHAPNSIITLMQSGY
KIRDRLLRPATVQVVKKP

Sequences:

>Translated_178_residues
MIDDNIENNEQTINDIAEEIVETANPEVTALKAEIEELKDKLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDVEVTNIIAGVQMTKDELDKVFHKHHIEEIKPEIGSMFDYNLHNAIAQIEHPDHAPNSIITLMQSGY
KIRDRLLRPATVQVVKKP
>Mature_178_residues
MIDDNIENNEQTINDIAEEIVETANPEVTALKAEIEELKDKLIRTTAEIDNTRKRLEKARDEAKDYAIATFAKELLNVSD
NLSRALAHKPANSDVEVTNIIAGVQMTKDELDKVFHKHHIEEIKPEIGSMFDYNLHNAIAQIEHPDHAPNSIITLMQSGY
KIRDRLLRPATVQVVKKP

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=179, Percent_Identity=31.8435754189944, Blast_Score=87, Evalue=8e-18,
Organism=Homo sapiens, GI40255109, Length=150, Percent_Identity=27.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1788967, Length=184, Percent_Identity=34.2391304347826, Blast_Score=83, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17552458, Length=153, Percent_Identity=37.2549019607843, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324806, Length=179, Percent_Identity=36.3128491620112, Blast_Score=106, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=35.8381502890173, Blast_Score=99, Evalue=2e-21,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 20172; Mature: 20172

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDDNIENNEQTINDIAEEIVETANPEVTALKAEIEELKDKLIRTTAEIDNTRKRLEKAR
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAKDYAIATFAKELLNVSDNLSRALAHKPANSDVEVTNIIAGVQMTKDELDKVFHKHHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEIKPEIGSMFDYNLHNAIAQIEHPDHAPNSIITLMQSGYKIRDRLLRPATVQVVKKP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MIDDNIENNEQTINDIAEEIVETANPEVTALKAEIEELKDKLIRTTAEIDNTRKRLEKAR
CCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DEAKDYAIATFAKELLNVSDNLSRALAHKPANSDVEVTNIIAGVQMTKDELDKVFHKHHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEIKPEIGSMFDYNLHNAIAQIEHPDHAPNSIITLMQSGYKIRDRLLRPATVQVVKKP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA